Initiation of hnRNPA1 Low-Complexity Domain Condensation Monitored by Dynamic Light Scattering
Abstract
:1. Introduction
2. Results
2.1. hnRNPA1LCD Monomers, Nano-Condensates, and Micro-Condensates Detected via DLS
2.2. Electrostatics Modulate Nano-Condensate Formation
2.3. 1,6-Hexanediol Completely Solubilizes Micro-Condensates, but Not Nano-Condensates
3. Discussion
4. Materials and Methods
4.1. hnRNPA1LCD Expression and Purification
4.2. Dynamic Light Scattering
4.3. Microscopy Imaging
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Tsoi, P.S.; Ferreon, J.C.; Ferreon, A.C.M. Initiation of hnRNPA1 Low-Complexity Domain Condensation Monitored by Dynamic Light Scattering. Int. J. Mol. Sci. 2024, 25, 6825. https://doi.org/10.3390/ijms25136825
Tsoi PS, Ferreon JC, Ferreon ACM. Initiation of hnRNPA1 Low-Complexity Domain Condensation Monitored by Dynamic Light Scattering. International Journal of Molecular Sciences. 2024; 25(13):6825. https://doi.org/10.3390/ijms25136825
Chicago/Turabian StyleTsoi, Phoebe S., Josephine C. Ferreon, and Allan Chris M. Ferreon. 2024. "Initiation of hnRNPA1 Low-Complexity Domain Condensation Monitored by Dynamic Light Scattering" International Journal of Molecular Sciences 25, no. 13: 6825. https://doi.org/10.3390/ijms25136825