Beni-Suef University Journal of Basic and Applied Sciences, 2017
Abstract Follow-up surveys of the blood parasites of the livestock and their possible vectors are... more Abstract Follow-up surveys of the blood parasites of the livestock and their possible vectors are recommended to assess their distribution and infection rates in different areas in Egypt and to plan control measures against their vectors. By this study, the authors have completed a comprehensive survey of the extent of Trypanosoma evansi infection in North-West Egypt from November 2014 to October 2015, using blood film examination and RoTat1.2 PCR-based assay. The present study was carried out in the frame of PROCAMED project, supported by the European Union (ENPI-Joint operational Programme of the Mediterranean Basin-IEVP-CT). Historical data of our previous and the present survey have shown that trypanosomiasis in this particular area is constant and not subject to change over time. In addition, the present study has clarified that T. evansi is endemic in this area due to the interaction of a wide range of factors and features of the host which determine patterns of trypanosomiasis, and its distribution coincides with the distribution of vectors.
2 Abstract: The present study was conducted to evaluate the use of polymerase chain reaction (PCR... more 2 Abstract: The present study was conducted to evaluate the use of polymerase chain reaction (PCR) using (Rode Trypanozoon antigen type) RoTat 1.2 primers for the genomic DNA of -eight Egyptian strains of T. evansi from camels, goats, sheep, cattle and donkeys from different areas of Egypt. In addition, six previously identified isolates of T. evansi, from Africa, Asia and South-America, isolated from different hosts. Besides, one T. equiperdum , one T.b.brucei and two T. brucei subspp strains from Africa were used as reference strains. The present results support previous suggestions that PCR amplification of the RoTat 1.2 (Variable surface glycoprotein) VSG gene is a specific marker for reference T. evansi strains, but it failed to differentiate some Egyptian strains that have been previously identified as T. evansi . However, it identified natural populations of T. evansi in Egypt with degree of heterogeneity, describing for the first time that those natural populations revealed ...
In Egypt, two local strains of dromedaries' camels (Sudanese and Maghrabi) are different from... more In Egypt, two local strains of dromedaries' camels (Sudanese and Maghrabi) are different from each other in some morphological, physiological properties and their origin. Trypanosoma evansi was well studied in imported and local Sudanese camels. No published studies exist on trypanosomosis in Maghrabi camels originating from North Africa, despite serious epidemics which have occurred in recent years. For the first time, an epidemiological survey was carried out from May 2012 to July 2013 in Northern-West Coast, Egypt to assess prevalence of T. evansi infection in Maghrabi dromedary camels. Some mainly parasite-related epidemiological factors that may play a role in the distribution and impact of trypanosomiasis were determined with special attention to the specific epidemiological situation found in the study area. Blood film examination (B. film) and polymerase chain reaction (PCR) using two specific primers (RoTat 1.2 and TBR1.2) based assays optimized for the detection of T. ...
Neither physiological nor pathological changes following treatments explained why trypanosomes in... more Neither physiological nor pathological changes following treatments explained why trypanosomes in the same group of experimentally treated animals correlated in virulence. Also, they behaved like each other but not similar to other groups despite the same T. evansi injected strain. The current study aims to discuss whether molecular changes might occur to Trypanosoma evansi isolates followed treatments are responsible for that difference or not. Ten preserved isolates from T. evansi after previous treatments besides the original strain of T. evansi that injected before treatments were used in the present study. These isolates were intraperitoneally inoculated in 11 groups of male Wister Albino rats with equal doses. Parasitological findings and the molecular changes accompanied were discussed along with the experiment based on PCR-TR3/TR4 specific-primers. The study also achieved alignments, gene sequence and phylogenetic analysis for submitted and reference strains belong to T. eva...
Beni-Suef University Journal of Basic and Applied Sciences, 2017
Abstract Follow-up surveys of the blood parasites of the livestock and their possible vectors are... more Abstract Follow-up surveys of the blood parasites of the livestock and their possible vectors are recommended to assess their distribution and infection rates in different areas in Egypt and to plan control measures against their vectors. By this study, the authors have completed a comprehensive survey of the extent of Trypanosoma evansi infection in North-West Egypt from November 2014 to October 2015, using blood film examination and RoTat1.2 PCR-based assay. The present study was carried out in the frame of PROCAMED project, supported by the European Union (ENPI-Joint operational Programme of the Mediterranean Basin-IEVP-CT). Historical data of our previous and the present survey have shown that trypanosomiasis in this particular area is constant and not subject to change over time. In addition, the present study has clarified that T. evansi is endemic in this area due to the interaction of a wide range of factors and features of the host which determine patterns of trypanosomiasis, and its distribution coincides with the distribution of vectors.
2 Abstract: The present study was conducted to evaluate the use of polymerase chain reaction (PCR... more 2 Abstract: The present study was conducted to evaluate the use of polymerase chain reaction (PCR) using (Rode Trypanozoon antigen type) RoTat 1.2 primers for the genomic DNA of -eight Egyptian strains of T. evansi from camels, goats, sheep, cattle and donkeys from different areas of Egypt. In addition, six previously identified isolates of T. evansi, from Africa, Asia and South-America, isolated from different hosts. Besides, one T. equiperdum , one T.b.brucei and two T. brucei subspp strains from Africa were used as reference strains. The present results support previous suggestions that PCR amplification of the RoTat 1.2 (Variable surface glycoprotein) VSG gene is a specific marker for reference T. evansi strains, but it failed to differentiate some Egyptian strains that have been previously identified as T. evansi . However, it identified natural populations of T. evansi in Egypt with degree of heterogeneity, describing for the first time that those natural populations revealed ...
In Egypt, two local strains of dromedaries' camels (Sudanese and Maghrabi) are different from... more In Egypt, two local strains of dromedaries' camels (Sudanese and Maghrabi) are different from each other in some morphological, physiological properties and their origin. Trypanosoma evansi was well studied in imported and local Sudanese camels. No published studies exist on trypanosomosis in Maghrabi camels originating from North Africa, despite serious epidemics which have occurred in recent years. For the first time, an epidemiological survey was carried out from May 2012 to July 2013 in Northern-West Coast, Egypt to assess prevalence of T. evansi infection in Maghrabi dromedary camels. Some mainly parasite-related epidemiological factors that may play a role in the distribution and impact of trypanosomiasis were determined with special attention to the specific epidemiological situation found in the study area. Blood film examination (B. film) and polymerase chain reaction (PCR) using two specific primers (RoTat 1.2 and TBR1.2) based assays optimized for the detection of T. ...
Neither physiological nor pathological changes following treatments explained why trypanosomes in... more Neither physiological nor pathological changes following treatments explained why trypanosomes in the same group of experimentally treated animals correlated in virulence. Also, they behaved like each other but not similar to other groups despite the same T. evansi injected strain. The current study aims to discuss whether molecular changes might occur to Trypanosoma evansi isolates followed treatments are responsible for that difference or not. Ten preserved isolates from T. evansi after previous treatments besides the original strain of T. evansi that injected before treatments were used in the present study. These isolates were intraperitoneally inoculated in 11 groups of male Wister Albino rats with equal doses. Parasitological findings and the molecular changes accompanied were discussed along with the experiment based on PCR-TR3/TR4 specific-primers. The study also achieved alignments, gene sequence and phylogenetic analysis for submitted and reference strains belong to T. eva...
Uploads