The purpose of this study was to clarify the role of the intestinal anaerobe bacteria colonizing ... more The purpose of this study was to clarify the role of the intestinal anaerobe bacteria colonizing the intestine of the newborn delivered by cesarean section. The intestinal bacterial colonization of 18 newborns delivered by cesarean section was assessed sequentially over the first 14 days of life. Emphasis is placed on C. perfringens and upon interaction to other bacteria. Correlation was made with the infant feeding mode. Microbial studies were realized in anaerobic conditions using Columbia blood agar simple and supplemented with antibiotics. Artificial alimentation seemed to favour C. perfringens installation, in contrast to the maternal one which promote B. bifidum proliferation. Antagonism between these bacteria seems to be established in the newborn intestine, via the alimentation.
The purpose of this study was to clarify the role of the intestinal anaerobic bacteria colonizing... more The purpose of this study was to clarify the role of the intestinal anaerobic bacteria colonizing the intestine of the newborn delivered by cesarean section. Control of the intestinal microecology is dependent on many factors including intestinal peristalsis, the intraluminal environment, and microbial interactions, that deter the overgrowth of pathogens populations. Numerous factors help achieve this normal balance. The effect of feeding seems to induce bacteriological changes.
Colonization of the digestive tract by C. perfringens was studied in infants born by cesarean sec... more Colonization of the digestive tract by C. perfringens was studied in infants born by cesarean section. Correlations between the level of colonization and the environment, type of feeding, and presence of other anaerobic bacteria were looked for. Colonization by C. perfringens was found as early as the second day of life in one of the maternity wards studied, suggesting presence of the microorganism in the environment. By the 14th day of life, colonization with C. perfringens was demonstrated in all the bottle-fed or breast and bottle-fed infants. In strictly breast-fed infants, findings suggested antagonism between Bifidobacterium and C. perfringens. Bacteroïdes and Clostridium species other than C. perfringens were not found prior to colonization by C. perfringens.
It has been established that colonization of the gastrointestinal tract by Enterobacteriaceae and... more It has been established that colonization of the gastrointestinal tract by Enterobacteriaceae and especially E. coli in newborn delivered by caesarean section occurs generally within the second day of life. On the 14th day, these bacteria inhabit the fecal flora of the majority of newborn. Hospital environment, as well as type of feeding do not seem to influence their frequency nor their level in the intestine of the infants born by caesarean section.
Lactobacillus paracasei K5 is a lactic acid bacteria (LAB) strain, isolated recently from feta-ty... more Lactobacillus paracasei K5 is a lactic acid bacteria (LAB) strain, isolated recently from feta-type cheese. Its probiotic potential has been demonstrated in a series of established in vitro tests. Moreover, incorporation of L. paracasei K5 as starter culture offered organoleptic and technological advantages to novel fermented food products. In the present study, further investigation of the potential probiotic activity of L. paracasei K5 was performed and its mechanisms of action were investigated. Employing quantitative analysis and confocal, fluorescent microscopy the adhesion properties of the above strain were studied. L. paracasei K5 displayed efficient adherence capacity to Caco-2 colon cancer cells, similarly to the reference strains Lactobacillus casei ATCC 393 and Lactobacillus rhamnosus GG. Moreover, treatment of Caco-2 cells with L. paracasei K5 inhibited cell proliferation in a time-and dose-dependent manner. The anti-proliferative effects appear to be mediated through induction of apoptosis via modulation of expression of specific Bcl-2 family proteins. These results elucidate the mechanisms of action of L. paracasei K5 and enhance its potential probiotic activity.
The purpose of this study was to clarify the role of the intestinal anaerobe bacteria colonizing ... more The purpose of this study was to clarify the role of the intestinal anaerobe bacteria colonizing the intestine of the newborn delivered by cesarean section. The intestinal bacterial colonization of 18 newborns delivered by cesarean section was assessed sequentially over the first 14 days of life. Emphasis is placed on C. perfringens and upon interaction to other bacteria. Correlation was made with the infant feeding mode. Microbial studies were realized in anaerobic conditions using Columbia blood agar simple and supplemented with antibiotics. Artificial alimentation seemed to favour C. perfringens installation, in contrast to the maternal one which promote B. bifidum proliferation. Antagonism between these bacteria seems to be established in the newborn intestine, via the alimentation.
The purpose of this study was to clarify the role of the intestinal anaerobic bacteria colonizing... more The purpose of this study was to clarify the role of the intestinal anaerobic bacteria colonizing the intestine of the newborn delivered by cesarean section. Control of the intestinal microecology is dependent on many factors including intestinal peristalsis, the intraluminal environment, and microbial interactions, that deter the overgrowth of pathogens populations. Numerous factors help achieve this normal balance. The effect of feeding seems to induce bacteriological changes.
Colonization of the digestive tract by C. perfringens was studied in infants born by cesarean sec... more Colonization of the digestive tract by C. perfringens was studied in infants born by cesarean section. Correlations between the level of colonization and the environment, type of feeding, and presence of other anaerobic bacteria were looked for. Colonization by C. perfringens was found as early as the second day of life in one of the maternity wards studied, suggesting presence of the microorganism in the environment. By the 14th day of life, colonization with C. perfringens was demonstrated in all the bottle-fed or breast and bottle-fed infants. In strictly breast-fed infants, findings suggested antagonism between Bifidobacterium and C. perfringens. Bacteroïdes and Clostridium species other than C. perfringens were not found prior to colonization by C. perfringens.
It has been established that colonization of the gastrointestinal tract by Enterobacteriaceae and... more It has been established that colonization of the gastrointestinal tract by Enterobacteriaceae and especially E. coli in newborn delivered by caesarean section occurs generally within the second day of life. On the 14th day, these bacteria inhabit the fecal flora of the majority of newborn. Hospital environment, as well as type of feeding do not seem to influence their frequency nor their level in the intestine of the infants born by caesarean section.
Lactobacillus paracasei K5 is a lactic acid bacteria (LAB) strain, isolated recently from feta-ty... more Lactobacillus paracasei K5 is a lactic acid bacteria (LAB) strain, isolated recently from feta-type cheese. Its probiotic potential has been demonstrated in a series of established in vitro tests. Moreover, incorporation of L. paracasei K5 as starter culture offered organoleptic and technological advantages to novel fermented food products. In the present study, further investigation of the potential probiotic activity of L. paracasei K5 was performed and its mechanisms of action were investigated. Employing quantitative analysis and confocal, fluorescent microscopy the adhesion properties of the above strain were studied. L. paracasei K5 displayed efficient adherence capacity to Caco-2 colon cancer cells, similarly to the reference strains Lactobacillus casei ATCC 393 and Lactobacillus rhamnosus GG. Moreover, treatment of Caco-2 cells with L. paracasei K5 inhibited cell proliferation in a time-and dose-dependent manner. The anti-proliferative effects appear to be mediated through induction of apoptosis via modulation of expression of specific Bcl-2 family proteins. These results elucidate the mechanisms of action of L. paracasei K5 and enhance its potential probiotic activity.
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Papers by Eugenia Bezirtzoglou