Summary
The prasinophycean flagellatePyramimonas orientalis has been examined by light and electron microscopy of wild and cultured material. The many different scales which cover all cell surfaces, including the flagella, are described; their synthesis and assembly in the two Golgi bodies have been examined. The Golgi bodies work simultaneously to produce all-at least five—scale categories, including hollow hair shaped scales. From the Golgi system the scales become transported to a special container—a reservoir—in which they, in an unknown way, separate and become arranged in the same pattern as on the body surface. From the reservoir, the scales move through a duct to the cell surface, apparently together with the subtending membrane, which thus becomes incorporated in the plasmalemma or the flagellar membrane. The liberation process, which differs from that of other species ofPyramimonas examined, is illustrated diagrammatically, starting at two extensions of ER from the nuclear envelope.
The flagellar apparatus possesses a flagellar root system of the green algal type, a finding of phylogenetic significance. Furthermore, near the flagellar transition region a structure was observed, which at present is known from certain “brown” groups of algae, but never from any green flagellate. The taxonomic implications are discussed briefly, and a virus attacking the nuclear area of the cell is reported. Very surprisingly two different sizes of the virus were found, which may be different stages of the same “organism”.
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References
Belcher, J. H., 1969: Further observations on the type species ofPyramimonas (P. tetrarhynchus Schmarda) (Prasinophyceae): an examination by light microscopy, together with notes on its taxonomy. Bot. J. Linn. Soc.62, 241–253.
Bouck, G. B., 1971: The structure, origin, isolation, and composition of the tubular mastigonemes of theOchromonas flagellum. J. Cell Biol.50, 362–384.
—, 1972: Architecture and assembly of mastigonemes. In: Advances in cell and molecular biology, Vol.2, pp. 237–271 (E. J. DuPraw, ed.). New York-London: Academic Press.
Butcher, R. W., 1959: An introductory account of the smaller algae of the British coastal waters. Part 1: Introduction andChlorophyceae. London: Her Majesty's Stationary Office.
Carter, N., 1938: New or interesting algae from brackish water. Arch. Protistenk.90, 1–68.
Hibberd, D. J., andG. F. Leedale, 1972: Observations on the cytology and ultrastructure of the new algal classEustigmatophyceae. Ann. Bot.36, 49–71.
Maiwald, M., 1971: A comparative ultrastructural study ofPyramimonas montana Geitler and aPyramimonas spec. Arch. Protistenk.113, 334–344.
Manton, I., 1964: Observations on the fine structure of the zoospore and young germling ofStigeoclonium. J. exp. Bot.15, 399–411.
—, 1966: Observations on scale production inPyramimonas amylifera Conrad. J. Cell Sci.1, 429–438.
—, 1967: Further observations on the fine structure ofChrysochromulina chiton with special reference to the haptonema, “peculiar” Golgi structure and scale production. J. Cell Sci.2, 265–272.
—, 1968: Observations on the microanatomy of the type species ofPyramimonas (P. tetrarhynchus Schmarda). Proc. Linn. Soc. (Lond.)179, 147–152.
—, 1969: Tubular trichocysts in a species ofPyramimonas (P. grossii Parke). Öst. bot. Z.116, 378–392.
Manton, I., andK. Harris, 1966: Observations on the microanatomy of the brown flagellateSphaleromantis tetragona Skuja with special reference to the flagellar apparatus and scales. Bot. J. Linn. Soc.59, 397–403.
—,K. Oates, andM. Parke, 1963: Observations on the fine structure of thePyramimonas stage ofHalosphaera and preliminary observations on three species ofPyramimonas. J. mar. biol. Ass. U.K.43, 225–238.
—,D. G. Rayns, H. Ettl, andM. Parke, 1965: Further observations on green flagellates with scaly flagella: the genusHeteromastix Korshikov. J. mar. biol. Ass. U.K.45, 241–255.
Marchant, H. J., J. D.Pickett-Heaps, and K.Jacobs, 1973: An ultrastructural study of zoosporogenesis and the mature zoospore ofKlebsormidium flaccidum. Cytobios (in press).
Massalski, A., 1969: Cytological studies on filamentousXanthophyceae. Ph. D., Leeds University.
Mattox, K. R., andK. D. Stewart, 1973: Observations on the zoospores ofPseudendoclonium basiliense andTrichosarcina polymorpha (Chlorophyceae). Canad. J. Bot.51, 1425–1430.
— — andG. L. Floyd, 1972: Probable virus infections in four genera of green algae. Canad. J. Microbiol.18, 1620–1621.
McBride, G. E., 1971: The flagellar base inColeochaete and its evolutionary significance. J. Phycol.7, Suppl., 13.
Moestrup, Ø., 1970: The fine structure of mature spermatozoids ofChara corallina, with special reference to microtubules and scales. Planta93, 295–308.
- 1974: Ultrastructure of the scale covered zoospores of the green algaChaetosphaeridium, a possible ancestor of the higher plants and bryophytes. Bot. J. Linn. Soc. (in press).
—, andL. R. Hoffman, 1973: Ultrastructure of the green algaDichotomosiphon tuberosus with special reference to the occurrence of striated tubules in the chloroplast. J. Phycol.9, 430–437.
Morré, D. J., H. H. Mollenhauer, andC. E. Bracker, 1971: Origin and continuity of Golgi apparatus. In: Origin and continuity of cell organelles, pp. 82–126. (J. Reinert andH. Ursprung, eds.). Berlin-Heidelberg-New York: Springer-Verlag.
Nielsen, R., 1972: A study of the shell-boring marine algae around the Danish island Læsø. Bot. Tidsskr.67, 245–269.
Olson, L. W., andG. Kochert, 1970: Ultrastructure ofVolvox carteri. II. The kinetosome. Arch. Mikrobiol.74, 31–40.
Pickett-Heaps, J. D., 1968: Ultrastructure and differentiation inChara (fibrosa). IV. Spermatogenesis. Aust. J. biol. Sci.21, 655–690.
—, andH. J. Marchant, 1972: The phylogeny of the green algae: a new proposal. Cytobios6, 255–264.
Ringo, D. L., 1967: Flagellar motion and fine structure of the flagellar apparatus inChlamydomonas. J. Cell Biol.33, 543–571.
Swale, E. M. F., 1973: A third layer of body scales inPyramimonas tetrarhynchus Schmarda. Br. Phycol. J.8, 95–99.
Throndsen, J., 1969: Flagellates of Norwegian coastal waters. Nytt Mag. Bot.16, 161–216.
Turner, F. R., 1968: An ultrastructural study of plant spermatogenesis. Spermatogenesis InNitella. J. Cell Biol.37, 370–393.
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Moestrup, Ø., Thomsen, H.A. An ultrastructural study of the flagellatePyramimonas orientalis with particular emphasis on Golgi apparatus activity and the flagellar apparatus. Protoplasma 81, 247–269 (1974). https://doi.org/10.1007/BF01275815
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DOI: https://doi.org/10.1007/BF01275815