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Inhibitory phlorotannins from the edible brown algaecklonia stolonifera on total reactive oxygen species (ROS) generation

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Abstract

Reactive oxygen species (ROS) play an important role in the pathogenesis of many human degenerative diseases such as cancer, aging, arteriosclerosis, and rheumatism. Much attention has been focused on the development of safe and effective antioxidants. To discover sources of antioxidative activity in marine algae, extracts from 17 kinds of seaweed were screened for their inhibitory effect on total ROS generation in kidney homogenate using 2′,7′-dichlorofluorescein diacetate (DCFH-DA). ROS inhibition was seen in three species:Ulva pertusa, Symphyocladia latiuscula, andEcklonia stolonifera. At a final concentration of 25 μg/mL,U. pertusa inhibited 85.65±20.28% of total ROS generation,S. latiscula caused 50.63±0.09% inhibitory, and theEcklonia species was 44.30±7.33% inhibition.E. stolonifera Okamura (Laminariaceae), which belongs to the brown algae, has been further investigated because it is commonly used as a foodstuff in Korea. Five compounds, phloroglucinol (1), eckstolonol (2), eckol (3), phlorofucofuroeckol A (4), and dieckol (5), isolated from the ethyl acetate soluble fraction of the methanolic extract ofE. stolonifera inhibited total ROS generation.

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Authors and Affiliations

  1. Faculty of Food Science and Biotechnology, Pukyong National University, 608-737, Busan, Korea

    Hye Sook Kang & Jae Sue Choi

  2. College of Pharmacy, Pusan National University, 609-735, Busan, Korea

    Hae Young Chung & Ji Young Kim

  3. Department of Chemistry, Pukyong National University, 608-737, Busan, Korea

    Byeng Wha Son

  4. Korea Maritime University, 606-791, Busan, Korea

    Hyun Ah Jung

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  1. Hye Sook Kang
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Correspondence to Jae Sue Choi.

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Kang, H.S., Chung, H.Y., Kim, J.Y. et al. Inhibitory phlorotannins from the edible brown algaecklonia stolonifera on total reactive oxygen species (ROS) generation. Arch Pharm Res 27, 194–198 (2004). https://doi.org/10.1007/BF02980106

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