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Melatonin and tryptophan counteract lipid peroxidation and modulate superoxide dismutase activity in ringdove heterophils in vivo. Effect of antigen-induced activation and age

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Abstract

Aging is associated with an increased production of free radicals and alterations in the mechanisms of adaptation to stress. A number of studies have shown a causal connection between age-related oxidant/antioxidant imbalance and the diminution of an organism’s melatonin levels in old age. Restoration of this rhythm may contribute to the re-stabilization of cellular homeostasis. The present work was aimed at examining the effect of the administration of melatonin or its precursor, the amino acid tryptophan, on heterophil lipid peroxidation (LPO) levels and superoxide dismutase (SOD) activity in heterophils from young (4–5 year) and old (12–14 year) ringdoves (Streptopelia risoria) under both basal and antigen-induced (stressful) conditions. Young animals were treated for 3 consecutive days with a single daily oral dose (0.25 mg) of melatonin at 1900 hours while old animals were treated for 3 consecutive days with either an oral dose (2.5 mg) of melatonin at 1900 hours or an oral capsule of 300 mg/kg body weight of l-tryptophan at 0900 hours. Non-treated old animals presented lower circulating levels of melatonin at night and higher levels of malonaldehyde (MDA) both day and night when compared to young birds. In both age groups, LPO levels were lower at night than during the day. Melatonin or tryptophan treatments significantly increased serum melatonin levels, reinforced SOD activity, and reduced MDA levels induced by the antigen. Melatonin and tryptophan may be useful agents for the treatment of disease states and processes in which an excessive production of oxidative damage occurs.

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Acknowledgments

This investigation was supported by a research grant from Consejería de Infraestructuras y Desarrollo Tecnológico (Junta de Extremadura, 3PR05A053). S.D.P., I.B. and M.P.T. were beneficiaries of grants from Consejería de Economía, Comercio e Innovación – Fondo Social Europeo (Junta de Extremadura, POS07012, PRE06070, and REI07004, respectively). The authors would like to express their thanks to Elena Circujano Vadillo, for technical assistance, and to Professors Manuel Molina and Manuel Mota, members of the Department of Mathematics (Section of Statistics and Operations Research) of the Faculty of Science, for their advice and collaboration in the statistical analysis.

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Authors and Affiliations

  1. Department of Physiology (Neuroimmunophysiology Research Group), Faculty of Science, University of Extremadura, Avda. de Elvas, s/n, 06071, Badajoz, Spain

    Sergio D. Paredes, Ignacio Bejarano, María Pilar Terrón, Carmen Barriga & Ana B. Rodríguez

  2. Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio, TX, USA

    Russel J. Reiter

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  1. Sergio D. Paredes
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  2. Ignacio Bejarano
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  3. María Pilar Terrón
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  4. Carmen Barriga
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  5. Russel J. Reiter
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  6. Ana B. Rodríguez
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Correspondence to Sergio D. Paredes.

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Paredes, S.D., Bejarano, I., Terrón, M.P. et al. Melatonin and tryptophan counteract lipid peroxidation and modulate superoxide dismutase activity in ringdove heterophils in vivo. Effect of antigen-induced activation and age. AGE 31, 179–188 (2009). https://doi.org/10.1007/s11357-009-9107-2

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  • DOI: https://doi.org/10.1007/s11357-009-9107-2

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