The preparation of amorphous and co-amorphous systems (CAMs) effectively addresses the solubility... more The preparation of amorphous and co-amorphous systems (CAMs) effectively addresses the solubility and bioavailability issues of poorly water-soluble chemical entities. However, stress conditions imposed during common pharmaceutical processing (e.g., tableting) may cause the recrystallization of the systems, warranting close stability monitoring throughout production. This work aimed at assessing the water and heat stability of amorphous olanzapine (OLZ) and OLZ-CAMs when subject to wet granulation and pelletization. Starting materials and products were characterized using calorimetry, diffractometry and spectroscopy, and their performance behavior was evaluated by dissolution testing. The results indicated that amorphous OLZ was reconverted back to a crystalline state after exposure to water and heat; conversely, OLZ-CAMs stabilized with saccharin (SAC), a sulfonic acid, did not show any significant loss of the amorphous content, confirming the higher stability of OLZ in the CAM. Be...
Biochimica Et Biophysica Acta - Proteins And Proteomics, Mar 1, 1996
Colominic acid (CA), a α-(2 → 8) N-acetylneuraminic acid (sialic acid) polymer (average molecular... more Colominic acid (CA), a α-(2 → 8) N-acetylneuraminic acid (sialic acid) polymer (average molecular weight of 10 kDa) was activated by periodate oxidation of carbon 7 at the non-reducing end of the saccharide. The oxidized CA was then coupled to catalase by reductive amination in the presence of sodium cyanoborohydride. The extent of sialylation of catalase, estimated by ammonium sulfate
International journal of clinical pharmacy, Jan 24, 2016
Background Potentially inappropriate medications (PIMs) are often found in high proportion among ... more Background Potentially inappropriate medications (PIMs) are often found in high proportion among the elderly population. The STOPP criteria have been suggested to detect more PIMs in European elderly than the Beers criteria. Objective This study aimed to determine the prevalence of PIMs and potential prescribing omissions (PPOs) in a sample of Portuguese nursing homes residents. Setting Four elderly facilities in mainland Portugal Method A descriptive cross-sectional study was used. Elderly polypharmacy patients were included in the study and their medication (registered in patient clinical records) analysed using the Beers (2012 original version and 2008 version adapted to Portugal), STOPP (Screening Tool of Older Person's Prescriptions) and START (Screening Tool to Alert doctors to Right Treatment) criteria. Data were analysed using univariate and bivariate descriptive statistics, considering a confidence interval of 95 %. Prevalence of PIMs and PPOs. Results The sample includ...
The successful integration of hot-melt extrusion (HME) and fused deposition modelling (FDM) depen... more The successful integration of hot-melt extrusion (HME) and fused deposition modelling (FDM) depends on a better understanding of the impact of environmental conditions on the printability of formulations, since they significantly affect the properties of the raw materials, whose control is crucial to enable three-dimensional printing (3DP). Hence, the objective of this work was to investigate the correlation between the environmental settings and the properties of paroxetine (PRX)-loaded filaments, previously produced by HME, which affect printability by FDM. The influence of different drying methods of the physical mixtures (PMs) and HME-filaments (FILs) on the quality and printability of these products was also assessed. The printability of FILs was evaluated in terms of the water content, and the mechanical and thermal properties of the products. Stability studies and physicochemical, thermal, and in vitro dissolution tests were carried out on the 3D-printed tablets. Stability st...
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1996
Colominic acid (CA), a α-(2 → 8) N-acetylneuraminic acid (sialic acid) polymer (average molecular... more Colominic acid (CA), a α-(2 → 8) N-acetylneuraminic acid (sialic acid) polymer (average molecular weight of 10 kDa) was activated by periodate oxidation of carbon 7 at the non-reducing end of the saccharide. The oxidized CA was then coupled to catalase by reductive amination in the presence of sodium cyanoborohydride. The extent of sialylation of catalase, estimated by ammonium sulfate
of the poster presented at the 4th International Congress of CiiEM - "Health, Well-being and... more of the poster presented at the 4th International Congress of CiiEM - "Health, Well-being and Ageing in the XXI Century." 2-5 June 2019, Campus Egas Moniz, Monte de Caparica, PortugalN/
Poster presented at the 2nd International Congress of CiiEM: Translational Research and Innovatio... more Poster presented at the 2nd International Congress of CiiEM: Translational Research and Innovation in Human and Health Sciences. Egas Moniz, Caparica, Portugal, 11-13 June 2017N/
of the communication presented at the Controlled Release Society Virtual Annual Meeting, 29 June-... more of the communication presented at the Controlled Release Society Virtual Annual Meeting, 29 June-2 July 2020N/
A drug’s bioavailability, i.e., the extent to and rate at which it enters the systemic circulatio... more A drug’s bioavailability, i.e., the extent to and rate at which it enters the systemic circulation, thus accessing the site of action, is largely determined by the properties of the drug [...]
Poster presented at the AAPS Annual Meeting and Exposition (American Association of Pharmaceutica... more Poster presented at the AAPS Annual Meeting and Exposition (American Association of Pharmaceutical Scientists). October 25-29, 2015, Orlando (FL), USA"Purpose: Investigation of the potential of casein micellar formulations as drug vehicles in pediatrics."Fundação para a Ciência e a Tecnologia (PTDC/DTP-FTO/1057/2012
The preparation of amorphous and co-amorphous systems (CAMs) effectively addresses the solubility... more The preparation of amorphous and co-amorphous systems (CAMs) effectively addresses the solubility and bioavailability issues of poorly water-soluble chemical entities. However, stress conditions imposed during common pharmaceutical processing (e.g., tableting) may cause the recrystallization of the systems, warranting close stability monitoring throughout production. This work aimed at assessing the water and heat stability of amorphous olanzapine (OLZ) and OLZ-CAMs when subject to wet granulation and pelletization. Starting materials and products were characterized using calorimetry, diffractometry and spectroscopy, and their performance behavior was evaluated by dissolution testing. The results indicated that amorphous OLZ was reconverted back to a crystalline state after exposure to water and heat; conversely, OLZ-CAMs stabilized with saccharin (SAC), a sulfonic acid, did not show any significant loss of the amorphous content, confirming the higher stability of OLZ in the CAM. Be...
Biochimica Et Biophysica Acta - Proteins And Proteomics, Mar 1, 1996
Colominic acid (CA), a α-(2 → 8) N-acetylneuraminic acid (sialic acid) polymer (average molecular... more Colominic acid (CA), a α-(2 → 8) N-acetylneuraminic acid (sialic acid) polymer (average molecular weight of 10 kDa) was activated by periodate oxidation of carbon 7 at the non-reducing end of the saccharide. The oxidized CA was then coupled to catalase by reductive amination in the presence of sodium cyanoborohydride. The extent of sialylation of catalase, estimated by ammonium sulfate
International journal of clinical pharmacy, Jan 24, 2016
Background Potentially inappropriate medications (PIMs) are often found in high proportion among ... more Background Potentially inappropriate medications (PIMs) are often found in high proportion among the elderly population. The STOPP criteria have been suggested to detect more PIMs in European elderly than the Beers criteria. Objective This study aimed to determine the prevalence of PIMs and potential prescribing omissions (PPOs) in a sample of Portuguese nursing homes residents. Setting Four elderly facilities in mainland Portugal Method A descriptive cross-sectional study was used. Elderly polypharmacy patients were included in the study and their medication (registered in patient clinical records) analysed using the Beers (2012 original version and 2008 version adapted to Portugal), STOPP (Screening Tool of Older Person's Prescriptions) and START (Screening Tool to Alert doctors to Right Treatment) criteria. Data were analysed using univariate and bivariate descriptive statistics, considering a confidence interval of 95 %. Prevalence of PIMs and PPOs. Results The sample includ...
The successful integration of hot-melt extrusion (HME) and fused deposition modelling (FDM) depen... more The successful integration of hot-melt extrusion (HME) and fused deposition modelling (FDM) depends on a better understanding of the impact of environmental conditions on the printability of formulations, since they significantly affect the properties of the raw materials, whose control is crucial to enable three-dimensional printing (3DP). Hence, the objective of this work was to investigate the correlation between the environmental settings and the properties of paroxetine (PRX)-loaded filaments, previously produced by HME, which affect printability by FDM. The influence of different drying methods of the physical mixtures (PMs) and HME-filaments (FILs) on the quality and printability of these products was also assessed. The printability of FILs was evaluated in terms of the water content, and the mechanical and thermal properties of the products. Stability studies and physicochemical, thermal, and in vitro dissolution tests were carried out on the 3D-printed tablets. Stability st...
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1996
Colominic acid (CA), a α-(2 → 8) N-acetylneuraminic acid (sialic acid) polymer (average molecular... more Colominic acid (CA), a α-(2 → 8) N-acetylneuraminic acid (sialic acid) polymer (average molecular weight of 10 kDa) was activated by periodate oxidation of carbon 7 at the non-reducing end of the saccharide. The oxidized CA was then coupled to catalase by reductive amination in the presence of sodium cyanoborohydride. The extent of sialylation of catalase, estimated by ammonium sulfate
of the poster presented at the 4th International Congress of CiiEM - "Health, Well-being and... more of the poster presented at the 4th International Congress of CiiEM - "Health, Well-being and Ageing in the XXI Century." 2-5 June 2019, Campus Egas Moniz, Monte de Caparica, PortugalN/
Poster presented at the 2nd International Congress of CiiEM: Translational Research and Innovatio... more Poster presented at the 2nd International Congress of CiiEM: Translational Research and Innovation in Human and Health Sciences. Egas Moniz, Caparica, Portugal, 11-13 June 2017N/
of the communication presented at the Controlled Release Society Virtual Annual Meeting, 29 June-... more of the communication presented at the Controlled Release Society Virtual Annual Meeting, 29 June-2 July 2020N/
A drug’s bioavailability, i.e., the extent to and rate at which it enters the systemic circulatio... more A drug’s bioavailability, i.e., the extent to and rate at which it enters the systemic circulation, thus accessing the site of action, is largely determined by the properties of the drug [...]
Poster presented at the AAPS Annual Meeting and Exposition (American Association of Pharmaceutica... more Poster presented at the AAPS Annual Meeting and Exposition (American Association of Pharmaceutical Scientists). October 25-29, 2015, Orlando (FL), USA"Purpose: Investigation of the potential of casein micellar formulations as drug vehicles in pediatrics."Fundação para a Ciência e a Tecnologia (PTDC/DTP-FTO/1057/2012
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Papers by Ana Isabel Fernandes