Pseudomonas putida was acclimized to phenol by increase in concentration and the degradation rate... more Pseudomonas putida was acclimized to phenol by increase in concentration and the degradation rate was calculated as approximately 0.042gl−1h−1 in batch shake flask cultures. Phenol degradation by P. putida immobilized on several silica based support materials was investigated. Pumice was chosen as a support material and then partially characterized physically and chemically. The cell adsorption ratio was 91% with Zr-activated
The study aims to develop wash‐resistant antibacterial cotton fabrics without using synthetic che... more The study aims to develop wash‐resistant antibacterial cotton fabrics without using synthetic chemicals. Therefore, natural active agents of thyme, thymol and carvacrol were selected. The inclusion complexes were formed with β‐cyclodextrin using kneading method which is a simple and reproducible method for the encapsulation with high production yield. Differential scanning calorimeter analysis showed that 1:1 and 1:2 β‐CD: Guest Molecule (M:M) for thymol and carvacrol from different ratios studied has the highest complexation degree as 50% and 100%, respectively. It is also revealed that the volatile agents are retained and showed better thermal stability as a result of complexation. Carvacrol inclusion complexes were found relatively more stable (Zeta potential: −28.2 mV) than thymol complexes with smaller particle sizes (204.9 nm). Chemical structures of the inclusion complexes were revealed with Fourier transform‐infrared spectroscopy and nuclear magnetic resonance analyses. The optimum formulations for each active agent were applied to cotton fabrics as per the impregnation method and the capsule treated fabrics were washed 1, 10 and 20 times. The images exhibited the presence of inclusion complexes on the fabrics after 20 washing cycles. Although the antibacterial efficacy of fabrics decreased with increasing washing, the fabrics showed the antibacterial effect after 20 washes against Klebsiella pneumoniae and Staphylococcus aureus. This study showed that the developed products can be an alternative to the other products in the market as the long‐lasting fragrant natural antibacterial.
WOS: 000293193000042In this study; sub-tropical white rot fungi, Trametes versicolor was investig... more WOS: 000293193000042In this study; sub-tropical white rot fungi, Trametes versicolor was investigated for its ability to degrade fluorene in the mediums containing glucose and different concentrations of fluorene (5.0-50.0 mg/L) in batch systems. T versicolor was also studied for the ability to degrade fluorene in a medium has high saline concentrations. (20, 15, 10, 5, 3.5, 3, 2.5, 1.5 %). Samples were collected at weekly intervals for 6 weeks and fluorene was detected by high performance liquid chromatography. Laccase and Mn peroxidase enzyme activities were also monitored and not detectable at the end of the incubation in higher saline conditions (except 1.5 % salinity). The greatest biodegradation was obtained by T versicolor with 30.0 mg/L fluorene concentration in 3.5, 3.0, 2.5 and 1.5 % salinity medium
WOS: 000293193000052Suillus luteus is a pored, edible mushroom of the genus Suillus in the Suilla... more WOS: 000293193000052Suillus luteus is a pored, edible mushroom of the genus Suillus in the Suillaceae family in Boletales order. The wool fabrics were dyed with the ethanolic extract of S. luteus fruit bodies at different pH values with various mordants. The greatest colour yield was obtained at pH 3 without mordant. Highest K/S values were obtained at pH 5 and 6 mordanting with copper sulfate. However at pH 4, 3 and 2 the highest K/S values were obtained mordanting with tin(II) chloride. Although, all dyed samples have good washing and crocking fastness, poor washing fastness colour change values were observed at pH 2 and 3 without mordanting
... Alper AKKAYA ve Nurdan PAZARLIOĞLU Ege Üniversitesi Fen Fakültesi Biyokimya Bölümü Bornova-İZ... more ... Alper AKKAYA ve Nurdan PAZARLIOĞLU Ege Üniversitesi Fen Fakültesi Biyokimya Bölümü Bornova-İZMİR 35100 nurdan.pazarlioglu@ege.edu.tr ... Katalitik RNA (ribozimler) moleküllerinin küçük bir grubu hariç bütün enzimler proteindirler. ...
Laccase (benzenediol: oxygen oxidoreductase; EC 1.10.3.2) is a particularly promising enzyme for ... more Laccase (benzenediol: oxygen oxidoreductase; EC 1.10.3.2) is a particularly promising enzyme for several industrial fields, including food industries, since this enzyme catalyzes the oxidation of ortho and para-diphenols, amino-phenols, polyphenols, polyamines, lignins, and aryl diamines as well as some inorganic ions coupled to the reduction of molecular dioxygen to water. In this study, laccase was produced from one of the
Poly(3-hydroxybutyrate) is nontoxic and biodegradable, with good biocompatibility and potential s... more Poly(3-hydroxybutyrate) is nontoxic and biodegradable, with good biocompatibility and potential support for long-term implants. For this reason, it is a good support for enzyme immobilization. Enzyme immobilization could not be done directly because poly(3-hydroxybutyrate) has no functional groups. Therefore, modification should be done for enzyme immobilization. In this study, methacrylic acid was graft polymerized to poly(3-hydroxybutyrate) and thrombin was immobilized to polymethacrylic acid grafted poly(3-hydroxybutyrate). In fact, graft polymerization of methacrylic acid to poly(3-hydroxybutyrate) and thrombin immobilization was a model study. Biomolecule immobilized poly(3-hydroxybutyrate) could be used as an implant. Thrombin was selected as a biomolecule for this model study and it was immobilized to methacrylic acid grafted poly(3-hydroxybutyrate). Then the developed product was used to stop bleeding.
In the present study, the production of laccase (Lac) and manganese-dependent peroxidase (MnP) by... more In the present study, the production of laccase (Lac) and manganese-dependent peroxidase (MnP) by the white-rot fungus Trametes versicolor grown in submerged cultures with different agricultural residues was investigated. The lignocellulosic materials studied were almond ...
Pseudomonas putida was acclimized to phenol by increase in concentration and the degradation rate... more Pseudomonas putida was acclimized to phenol by increase in concentration and the degradation rate was calculated as approximately 0.042gl−1h−1 in batch shake flask cultures. Phenol degradation by P. putida immobilized on several silica based support materials was investigated. Pumice was chosen as a support material and then partially characterized physically and chemically. The cell adsorption ratio was 91% with Zr-activated
The study aims to develop wash‐resistant antibacterial cotton fabrics without using synthetic che... more The study aims to develop wash‐resistant antibacterial cotton fabrics without using synthetic chemicals. Therefore, natural active agents of thyme, thymol and carvacrol were selected. The inclusion complexes were formed with β‐cyclodextrin using kneading method which is a simple and reproducible method for the encapsulation with high production yield. Differential scanning calorimeter analysis showed that 1:1 and 1:2 β‐CD: Guest Molecule (M:M) for thymol and carvacrol from different ratios studied has the highest complexation degree as 50% and 100%, respectively. It is also revealed that the volatile agents are retained and showed better thermal stability as a result of complexation. Carvacrol inclusion complexes were found relatively more stable (Zeta potential: −28.2 mV) than thymol complexes with smaller particle sizes (204.9 nm). Chemical structures of the inclusion complexes were revealed with Fourier transform‐infrared spectroscopy and nuclear magnetic resonance analyses. The optimum formulations for each active agent were applied to cotton fabrics as per the impregnation method and the capsule treated fabrics were washed 1, 10 and 20 times. The images exhibited the presence of inclusion complexes on the fabrics after 20 washing cycles. Although the antibacterial efficacy of fabrics decreased with increasing washing, the fabrics showed the antibacterial effect after 20 washes against Klebsiella pneumoniae and Staphylococcus aureus. This study showed that the developed products can be an alternative to the other products in the market as the long‐lasting fragrant natural antibacterial.
WOS: 000293193000042In this study; sub-tropical white rot fungi, Trametes versicolor was investig... more WOS: 000293193000042In this study; sub-tropical white rot fungi, Trametes versicolor was investigated for its ability to degrade fluorene in the mediums containing glucose and different concentrations of fluorene (5.0-50.0 mg/L) in batch systems. T versicolor was also studied for the ability to degrade fluorene in a medium has high saline concentrations. (20, 15, 10, 5, 3.5, 3, 2.5, 1.5 %). Samples were collected at weekly intervals for 6 weeks and fluorene was detected by high performance liquid chromatography. Laccase and Mn peroxidase enzyme activities were also monitored and not detectable at the end of the incubation in higher saline conditions (except 1.5 % salinity). The greatest biodegradation was obtained by T versicolor with 30.0 mg/L fluorene concentration in 3.5, 3.0, 2.5 and 1.5 % salinity medium
WOS: 000293193000052Suillus luteus is a pored, edible mushroom of the genus Suillus in the Suilla... more WOS: 000293193000052Suillus luteus is a pored, edible mushroom of the genus Suillus in the Suillaceae family in Boletales order. The wool fabrics were dyed with the ethanolic extract of S. luteus fruit bodies at different pH values with various mordants. The greatest colour yield was obtained at pH 3 without mordant. Highest K/S values were obtained at pH 5 and 6 mordanting with copper sulfate. However at pH 4, 3 and 2 the highest K/S values were obtained mordanting with tin(II) chloride. Although, all dyed samples have good washing and crocking fastness, poor washing fastness colour change values were observed at pH 2 and 3 without mordanting
... Alper AKKAYA ve Nurdan PAZARLIOĞLU Ege Üniversitesi Fen Fakültesi Biyokimya Bölümü Bornova-İZ... more ... Alper AKKAYA ve Nurdan PAZARLIOĞLU Ege Üniversitesi Fen Fakültesi Biyokimya Bölümü Bornova-İZMİR 35100 nurdan.pazarlioglu@ege.edu.tr ... Katalitik RNA (ribozimler) moleküllerinin küçük bir grubu hariç bütün enzimler proteindirler. ...
Laccase (benzenediol: oxygen oxidoreductase; EC 1.10.3.2) is a particularly promising enzyme for ... more Laccase (benzenediol: oxygen oxidoreductase; EC 1.10.3.2) is a particularly promising enzyme for several industrial fields, including food industries, since this enzyme catalyzes the oxidation of ortho and para-diphenols, amino-phenols, polyphenols, polyamines, lignins, and aryl diamines as well as some inorganic ions coupled to the reduction of molecular dioxygen to water. In this study, laccase was produced from one of the
Poly(3-hydroxybutyrate) is nontoxic and biodegradable, with good biocompatibility and potential s... more Poly(3-hydroxybutyrate) is nontoxic and biodegradable, with good biocompatibility and potential support for long-term implants. For this reason, it is a good support for enzyme immobilization. Enzyme immobilization could not be done directly because poly(3-hydroxybutyrate) has no functional groups. Therefore, modification should be done for enzyme immobilization. In this study, methacrylic acid was graft polymerized to poly(3-hydroxybutyrate) and thrombin was immobilized to polymethacrylic acid grafted poly(3-hydroxybutyrate). In fact, graft polymerization of methacrylic acid to poly(3-hydroxybutyrate) and thrombin immobilization was a model study. Biomolecule immobilized poly(3-hydroxybutyrate) could be used as an implant. Thrombin was selected as a biomolecule for this model study and it was immobilized to methacrylic acid grafted poly(3-hydroxybutyrate). Then the developed product was used to stop bleeding.
In the present study, the production of laccase (Lac) and manganese-dependent peroxidase (MnP) by... more In the present study, the production of laccase (Lac) and manganese-dependent peroxidase (MnP) by the white-rot fungus Trametes versicolor grown in submerged cultures with different agricultural residues was investigated. The lignocellulosic materials studied were almond ...
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