The chromosomes of Chromacris nuptialis and C. speciosa were comparatively analyzed using differe... more The chromosomes of Chromacris nuptialis and C. speciosa were comparatively analyzed using different cytogenetic techniques, in order to determine the level of karyotypic similarities and differences between the species. The results show similarities in chromosome number (2n=23,X0) and acrocentric morphology. In some C. nuptialis individuals meiotic irregularities were detected involving the L(2) bivalent. This bivalent was delayed and presented anaphasic bridges and other aberrations. Differences in constitutive heterochromatin (CH) patterns and composition were observed through C-banding and fluorochromes staining. Silver nitrate staining revealed a single medium nucleolar organizer regions (NORs) pair, per species. Differences were also observed in NORs location, which was pericentromeric in C. nuptialis and proximal in C. speciosa. FISH using an rDNA probe confirmed the existence of ribosomal sites coinciding with active regions visualized by silver nitrate. The possible implications of the karyotype differences observed between both species are discussed.
... Maria José de Souza1 and Natoniel Franklin de Melo2 ... NOR numbers and distribution can repr... more ... Maria José de Souza1 and Natoniel Franklin de Melo2 ... NOR numbers and distribution can represent a good marker in the distinction between related species (Rufas et al., 1985; Cabrero and Camacho, 1986b; Rocha et al., 2004). ...
The chromosomes of Chromacris nuptialis and C. speciosa were comparatively analyzed using differe... more The chromosomes of Chromacris nuptialis and C. speciosa were comparatively analyzed using different cytogenetic techniques, in order to determine the level of karyotypic similarities and differences between the species. The results show similarities in chromosome number (2n=23,X0) and acrocentric morphology. In some C. nuptialis individuals meiotic irregularities were detected involving the L(2) bivalent. This bivalent was delayed and presented anaphasic bridges and other aberrations. Differences in constitutive heterochromatin (CH) patterns and composition were observed through C-banding and fluorochromes staining. Silver nitrate staining revealed a single medium nucleolar organizer regions (NORs) pair, per species. Differences were also observed in NORs location, which was pericentromeric in C. nuptialis and proximal in C. speciosa. FISH using an rDNA probe confirmed the existence of ribosomal sites coinciding with active regions visualized by silver nitrate. The possible implications of the karyotype differences observed between both species are discussed.
... Nicácio de Oliveira Freitas I ; Adriana Mayumi Yano-Melo II ; Fábio Sérgio Barbosa da Silva I... more ... Nicácio de Oliveira Freitas I ; Adriana Mayumi Yano-Melo II ; Fábio Sérgio Barbosa da Silva III ; Natoniel Franklin de Melo IV ; Leonor ... was used for physical and chemical characterization (Table 1). Another fraction (200 mL per container) was used for preparing trap cultures. ...
The chromosomes of Chromacris nuptialis and C. speciosa were comparatively analyzed using differe... more The chromosomes of Chromacris nuptialis and C. speciosa were comparatively analyzed using different cytogenetic techniques, in order to determine the level of karyotypic similarities and differences between the species. The results show similarities in chromosome number (2n=23,X0) and acrocentric morphology. In some C. nuptialis individuals meiotic irregularities were detected involving the L(2) bivalent. This bivalent was delayed and presented anaphasic bridges and other aberrations. Differences in constitutive heterochromatin (CH) patterns and composition were observed through C-banding and fluorochromes staining. Silver nitrate staining revealed a single medium nucleolar organizer regions (NORs) pair, per species. Differences were also observed in NORs location, which was pericentromeric in C. nuptialis and proximal in C. speciosa. FISH using an rDNA probe confirmed the existence of ribosomal sites coinciding with active regions visualized by silver nitrate. The possible implications of the karyotype differences observed between both species are discussed.
... Maria José de Souza1 and Natoniel Franklin de Melo2 ... NOR numbers and distribution can repr... more ... Maria José de Souza1 and Natoniel Franklin de Melo2 ... NOR numbers and distribution can represent a good marker in the distinction between related species (Rufas et al., 1985; Cabrero and Camacho, 1986b; Rocha et al., 2004). ...
The chromosomes of Chromacris nuptialis and C. speciosa were comparatively analyzed using differe... more The chromosomes of Chromacris nuptialis and C. speciosa were comparatively analyzed using different cytogenetic techniques, in order to determine the level of karyotypic similarities and differences between the species. The results show similarities in chromosome number (2n=23,X0) and acrocentric morphology. In some C. nuptialis individuals meiotic irregularities were detected involving the L(2) bivalent. This bivalent was delayed and presented anaphasic bridges and other aberrations. Differences in constitutive heterochromatin (CH) patterns and composition were observed through C-banding and fluorochromes staining. Silver nitrate staining revealed a single medium nucleolar organizer regions (NORs) pair, per species. Differences were also observed in NORs location, which was pericentromeric in C. nuptialis and proximal in C. speciosa. FISH using an rDNA probe confirmed the existence of ribosomal sites coinciding with active regions visualized by silver nitrate. The possible implications of the karyotype differences observed between both species are discussed.
... Nicácio de Oliveira Freitas I ; Adriana Mayumi Yano-Melo II ; Fábio Sérgio Barbosa da Silva I... more ... Nicácio de Oliveira Freitas I ; Adriana Mayumi Yano-Melo II ; Fábio Sérgio Barbosa da Silva III ; Natoniel Franklin de Melo IV ; Leonor ... was used for physical and chemical characterization (Table 1). Another fraction (200 mL per container) was used for preparing trap cultures. ...
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