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== Structure and function ==
== Structure and function ==
[[File:Bar Diagram of Full-length and Headless MyoX.png |thumb| Bar diagram illustrating the domain structures of full-length and headless Myo10 from human. IQ indicates the 3 IQ motifs, each of which binds to a calmodulin or calmodulin-like light chain. The IQ motifs are followed by an alpha-helical region consisting of an N-terminal segment that forms a single stable alpha helix (SAH) and a C-terminal segment that can dimerize by forming an antiparallel coiled coil (CC). See text for additional discussion and references. The approximate positions along the amino acid sequence of the major domains are based on.<ref>Berg et al., 2000</ref><ref>Hirano et al., 2011</ref><ref>Lu et al., 2011</ref><ref>Wei et al., 2011</ref> (Diagram courtesy of Joshua K. Zachariah and Richard E. Cheney)]]

The human ''MYO10'' gene spans ~274 kb and is located on chromosome 5 band 5p15.1 at 16,661,907-16,936,288 bp (GRCh Ensembl release 89). It produces a full-length RNA transcript with 41 [[exon]]s encoding a MYO10 heavy chain whose deduced sequence has 2058 amino acids and a predicted molecular weight of ~237 kDa. Like many motor proteins, the full-length Myo10 protein can be considered to consist of a head, neck, and tail.<ref name="Berg_2000" /><ref name="pmid22124140">{{cite journal | vauthors = Kerber ML, Cheney RE | title = Myosin-X: a MyTH-FERM myosin at the tips of filopodia | journal = Journal of Cell Science | volume = 124 | issue = Pt 22 | pages = 3733–41 | date = November 2011 | pmid = 22124140 | pmc = 3225264 | doi = 10.1242/jcs.023549 }}</ref> The N-terminal head or myosin motor domain can bind to an actin filament, hydrolyze [[Adenosine triphosphate|ATP]], and produce force.<ref name="Homma_2001">{{cite journal | vauthors = Homma K, Saito J, Ikebe R, Ikebe M | title = Motor function and regulation of myosin X | journal = The Journal of Biological Chemistry | volume = 276 | issue = 36 | pages = 34348–54 | date = September 2001 | pmid = 11457842 | doi = 10.1074/jbc.M104785200 | s2cid = 46029622 }}</ref><ref name="Takagi_2014">{{cite journal | vauthors = Takagi Y, Farrow RE, Billington N, Nagy A, Batters C, Yang Y, Sellers JR, Molloy JE | display-authors = 6 | title = Myosin-10 produces its power-stroke in two phases and moves processively along a single actin filament under low load | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 111 | issue = 18 | pages = E1833-42 | date = May 2014 | pmid = 24753602 | pmc = 4020102 | doi = 10.1073/pnas.1320122111 | bibcode = 2014PNAS..111E1833T }}</ref> The neck or light chain binding domain consists of 3 [[IQ calmodulin-binding motif|IQ motifs]], with each IQ motif providing a binding site for one molecule of [[calmodulin]], a ~16.5 kDa calcium-binding protein.<ref name="Homma_2001" /> Unlike most calmodulin binding sites, which only bind to calmodulin in the presence of calcium, the IQ motifs in Myo10 can bind to calmodulin in the absence of calcium. Each of the isolated Myo10 IQ motifs has also been reported to bind to [[CALML3]], a calmodulin-like protein expressed in epithelial cells, so CALML3 may serve as a Myo10 light chain in place of calmodulin in some situations.<ref name="Caride_2010">{{cite journal | vauthors = Caride AJ, Bennett RD, Strehler EE | title = Kinetic analysis reveals differences in the binding mechanism of calmodulin and calmodulin-like protein to the IQ motifs of myosin-10 | journal = Biochemistry | volume = 49 | issue = 37 | pages = 8105–16 | date = September 2010 | pmid = 20731332 | pmc = 3818122 | doi = 10.1021/bi100644q }}</ref> The Myo10 tail begins with an [[Alpha helix|alpha-helical]] region whose proximal portion forms a single, stable alpha helix (SAH domain) that lengthens the lever arm formed by the neck domain.<ref name="Knight_2005">{{cite journal | vauthors = Knight PJ, Thirumurugan K, Xu Y, Wang F, Kalverda AP, Stafford WF, Sellers JR, Peckham M | display-authors = 6 | title = The predicted coiled-coil domain of myosin 10 forms a novel elongated domain that lengthens the head | journal = The Journal of Biological Chemistry | volume = 280 | issue = 41 | pages = 34702–8 | date = October 2005 | pmid = 16030012 | doi = 10.1074/jbc.M504887200 | s2cid = 22503825 }}</ref><ref name="Lu_2012">{{cite journal | vauthors = Lu Q, Ye F, Wei Z, Wen Z, Zhang M | title = Antiparallel coiled-coil-mediated dimerization of myosin X | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 109 | issue = 43 | pages = 17388–93 | date = October 2012 | pmid = 23012428 | pmc = 3491486 | doi = 10.1073/pnas.1208642109 }}</ref> The distal portion of the alpha helical region can self-associate with a Kd of ~0.6 uM to form an antiparallel [[coiled coil]], allowing two Myo10 heavy chains to form an antiparallel dimer, a unique structure among known myosins.<ref name="Lu_2012" /><ref name="Ropars_2016">{{cite journal | vauthors = Ropars V, Yang Z, Isabet T, Blanc F, Zhou K, Lin T, Liu X, Hissier P, Samazan F, Amigues B, Yang ED, Park H, Pylypenko O, Cecchini M, Sindelar CV, Sweeney HL, Houdusse A | display-authors = 6 | title = The myosin X motor is optimized for movement on actin bundles | journal = Nature Communications | volume = 7 | issue = | pages = 12456 | date = September 2016 | pmid = 27580874 | pmc = 5025751 | doi = 10.1038/ncomms12456 | bibcode = 2016NatCo...712456R }}</ref>
The human ''MYO10'' gene spans ~274 kb and is located on chromosome 5 band 5p15.1 at 16,661,907-16,936,288 bp (GRCh Ensembl release 89). It produces a full-length RNA transcript with 41 [[exon]]s encoding a MYO10 heavy chain whose deduced sequence has 2058 amino acids and a predicted molecular weight of ~237 kDa. Like many motor proteins, the full-length Myo10 protein can be considered to consist of a head, neck, and tail.<ref name="Berg_2000" /><ref name="pmid22124140">{{cite journal | vauthors = Kerber ML, Cheney RE | title = Myosin-X: a MyTH-FERM myosin at the tips of filopodia | journal = Journal of Cell Science | volume = 124 | issue = Pt 22 | pages = 3733–41 | date = November 2011 | pmid = 22124140 | pmc = 3225264 | doi = 10.1242/jcs.023549 }}</ref> The N-terminal head or myosin motor domain can bind to an actin filament, hydrolyze [[Adenosine triphosphate|ATP]], and produce force.<ref name="Homma_2001">{{cite journal | vauthors = Homma K, Saito J, Ikebe R, Ikebe M | title = Motor function and regulation of myosin X | journal = The Journal of Biological Chemistry | volume = 276 | issue = 36 | pages = 34348–54 | date = September 2001 | pmid = 11457842 | doi = 10.1074/jbc.M104785200 | s2cid = 46029622 }}</ref><ref name="Takagi_2014">{{cite journal | vauthors = Takagi Y, Farrow RE, Billington N, Nagy A, Batters C, Yang Y, Sellers JR, Molloy JE | display-authors = 6 | title = Myosin-10 produces its power-stroke in two phases and moves processively along a single actin filament under low load | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 111 | issue = 18 | pages = E1833-42 | date = May 2014 | pmid = 24753602 | pmc = 4020102 | doi = 10.1073/pnas.1320122111 | bibcode = 2014PNAS..111E1833T }}</ref> The neck or light chain binding domain consists of 3 [[IQ calmodulin-binding motif|IQ motifs]], with each IQ motif providing a binding site for one molecule of [[calmodulin]], a ~16.5 kDa calcium-binding protein.<ref name="Homma_2001" /> Unlike most calmodulin binding sites, which only bind to calmodulin in the presence of calcium, the IQ motifs in Myo10 can bind to calmodulin in the absence of calcium. Each of the isolated Myo10 IQ motifs has also been reported to bind to [[CALML3]], a calmodulin-like protein expressed in epithelial cells, so CALML3 may serve as a Myo10 light chain in place of calmodulin in some situations.<ref name="Caride_2010">{{cite journal | vauthors = Caride AJ, Bennett RD, Strehler EE | title = Kinetic analysis reveals differences in the binding mechanism of calmodulin and calmodulin-like protein to the IQ motifs of myosin-10 | journal = Biochemistry | volume = 49 | issue = 37 | pages = 8105–16 | date = September 2010 | pmid = 20731332 | pmc = 3818122 | doi = 10.1021/bi100644q }}</ref> The Myo10 tail begins with an [[Alpha helix|alpha-helical]] region whose proximal portion forms a single, stable alpha helix (SAH domain) that lengthens the lever arm formed by the neck domain.<ref name="Knight_2005">{{cite journal | vauthors = Knight PJ, Thirumurugan K, Xu Y, Wang F, Kalverda AP, Stafford WF, Sellers JR, Peckham M | display-authors = 6 | title = The predicted coiled-coil domain of myosin 10 forms a novel elongated domain that lengthens the head | journal = The Journal of Biological Chemistry | volume = 280 | issue = 41 | pages = 34702–8 | date = October 2005 | pmid = 16030012 | doi = 10.1074/jbc.M504887200 | s2cid = 22503825 }}</ref><ref name="Lu_2012">{{cite journal | vauthors = Lu Q, Ye F, Wei Z, Wen Z, Zhang M | title = Antiparallel coiled-coil-mediated dimerization of myosin X | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 109 | issue = 43 | pages = 17388–93 | date = October 2012 | pmid = 23012428 | pmc = 3491486 | doi = 10.1073/pnas.1208642109 }}</ref> The distal portion of the alpha helical region can self-associate with a Kd of ~0.6 uM to form an antiparallel [[coiled coil]], allowing two Myo10 heavy chains to form an antiparallel dimer, a unique structure among known myosins.<ref name="Lu_2012" /><ref name="Ropars_2016">{{cite journal | vauthors = Ropars V, Yang Z, Isabet T, Blanc F, Zhou K, Lin T, Liu X, Hissier P, Samazan F, Amigues B, Yang ED, Park H, Pylypenko O, Cecchini M, Sindelar CV, Sweeney HL, Houdusse A | display-authors = 6 | title = The myosin X motor is optimized for movement on actin bundles | journal = Nature Communications | volume = 7 | issue = | pages = 12456 | date = September 2016 | pmid = 27580874 | pmc = 5025751 | doi = 10.1038/ncomms12456 | bibcode = 2016NatCo...712456R }}</ref>



Revision as of 19:33, 11 April 2021

MYO10
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesMYO10, myosin X, MyoX
External IDsOMIM: 601481; MGI: 107716; HomoloGene: 36328; GeneCards: MYO10; OMA:MYO10 - orthologs
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_012334

NM_019472
NM_001353141
NM_001353142

RefSeq (protein)

NP_036466

NP_062345
NP_001340070
NP_001340071

Location (UCSC)Chr 5: 16.66 – 16.94 MbChr 15: 25.62 – 25.81 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Myosin X, also known as MYO10 or Myo10, is a protein that in humans is encoded by the MYO10 gene.[5][6][7][8]

Myo10 is an actin-based motor protein that can localize to the tips of the finger-like cellular protrusions known as filopodia.[9][10] Myo10 is broadly expressed in mammalian tissues, although at relatively low levels.[8] Studies with knockout mice demonstrate that Myo10 has important functions in embryonic processes such as neural tube closure and eye development.[11][12][13] Myo10 also has important functions in cancer invasion and growth.[14][15][9][12][16][17]

Myo10 should be confused with Myh10, which encodes the heavy chain of the class II myosin known as non-muscle myosin 2b.

Structure and function

Bar diagram illustrating the domain structures of full-length and headless Myo10 from human. IQ indicates the 3 IQ motifs, each of which binds to a calmodulin or calmodulin-like light chain. The IQ motifs are followed by an alpha-helical region consisting of an N-terminal segment that forms a single stable alpha helix (SAH) and a C-terminal segment that can dimerize by forming an antiparallel coiled coil (CC). See text for additional discussion and references. The approximate positions along the amino acid sequence of the major domains are based on.[18][19][20][21] (Diagram courtesy of Joshua K. Zachariah and Richard E. Cheney)

The human MYO10 gene spans ~274 kb and is located on chromosome 5 band 5p15.1 at 16,661,907-16,936,288 bp (GRCh Ensembl release 89). It produces a full-length RNA transcript with 41 exons encoding a MYO10 heavy chain whose deduced sequence has 2058 amino acids and a predicted molecular weight of ~237 kDa. Like many motor proteins, the full-length Myo10 protein can be considered to consist of a head, neck, and tail.[8][22] The N-terminal head or myosin motor domain can bind to an actin filament, hydrolyze ATP, and produce force.[23][24] The neck or light chain binding domain consists of 3 IQ motifs, with each IQ motif providing a binding site for one molecule of calmodulin, a ~16.5 kDa calcium-binding protein.[23] Unlike most calmodulin binding sites, which only bind to calmodulin in the presence of calcium, the IQ motifs in Myo10 can bind to calmodulin in the absence of calcium. Each of the isolated Myo10 IQ motifs has also been reported to bind to CALML3, a calmodulin-like protein expressed in epithelial cells, so CALML3 may serve as a Myo10 light chain in place of calmodulin in some situations.[25] The Myo10 tail begins with an alpha-helical region whose proximal portion forms a single, stable alpha helix (SAH domain) that lengthens the lever arm formed by the neck domain.[26][27] The distal portion of the alpha helical region can self-associate with a Kd of ~0.6 uM to form an antiparallel coiled coil, allowing two Myo10 heavy chains to form an antiparallel dimer, a unique structure among known myosins.[27][28]

The Myo10 tail includes several regions in addition to the SAH and coiled coil. These include a region with 3 PEST sequences--sequences enriched in the amino acids Proline (P), Glutamine (E), Serine (S), and T (Threonine) that are often associated with cleavage by proteases such as calpain.[8] The Myo10 tail is unique among known myosins in containing 3 PH domains (Pleckstrin Homology domain), a domain often involved in binding to membranes. The sequence of Myo10’s first PH domain is somewhat unusual in that it is split by the presence of a surface loop that contains the second PH domain.[8][27] The second PH domain binds to the important signaling lipid phosphatidylinositol (3,4,5)-trisphosphate [PI(3,4,5)P3] and in some situations has been reported to bind to phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2].[29][27][30] Myo10’s 3 PH domains are thought to work together to recruit it to the plasma membrane. The Myo10 tail ends in a supramodule consisting of a MyTH4 domain (Myosin Tail Homology 4) and a FERM  domain (band 4.1, Ezrin, Radixin, Moesin).[31][32] Myo10’s MyTH4 domain can bind to microtubules with a reported affinity of ~0.24 uM and gives full-length Myo10 the important ability to link an actin filament bound by its head to a microtubule bound by its tail.[33][31][34] The Myo10 FERM domain can bind to the cytoplasmic domains of several β-integrins, a major class of cell adhesion receptor, and to the cytoplasmic domains of the netrin receptors Deleted in Colorectal Cancer (DCC) and neogenin (Neo1).[35][36] Although full-length Myo10 protein appears to be expressed at relatively low levels, it can be detected in most mammalian tissues including brain, testes, kidney, lung, stomach, and pancreas.[8]

The native full-length Myo10 heavy chain can exist as a monomer with 3 calmodulin/calmodulin-like light chains or as an antiparallel dimer with 6 calmodulin/calmodulin-like light chains. An antiparallel Myo10 dimer with all 6 light chains would thus have 8 subunits and a native MW of ~574 kDa. Importantly, the tail in a Myo10 monomer can fold back onto the head to inhibit the head’s motor activity.[30] Increases in plasma membrane PI(3,4,5)P3  levels are hypothesized to recruit Myo10 monomers to the plasma membrane via their PH domains, activating their motor activity and increasing their local concentration, leading to the formation of active antiparallel dimers that are capable moving along actin filaments. Myo10, like all known myosins other than Myo6, moves towards the barbed end of the actin filament.[37] Myo10 is capable of hydrolyzing ~10-20 ATP/s per head and has been reported to generate movement at rates of ~300-1500 nm/s.[24][28] Single-molecule studies show that native Myo10 dimers can take steps of up to ~55 nm, which are among the largest steps reported for a motor protein.[28] Myo10’s large step size is due in part to the long lever arm formed by its neck domain and stable alpha helix, and in part due to the remarkably large swing of ~120° the Myo10 lever arm undergoes during its power stroke.[28] There is much interest in the mechanisms that target Myo10 to filopodial actin bundles, and in Myo10’s ability to step from one actin filament in a bundle to another.[38][28][39] In addition to the full-length Myo10 described above, the use of alternative transcription start sites located in intron 19-20 of the full-length transcript results in the production of “headless” Myo10 transcripts that lack most of the myosin head domain, but include the rest of the Myo10 heavy chain.[40][41][42] The major headless transcripts in human are predicted to include exons 20-41 of full-length MYO10 and initiation of translation at M644 would result in a 1415 amino acid headless protein with a predicted MW of ~163 kDa that would be identical to amino acids 644-2058 of full-length MYO10.[40] Because headless Myo10 lacks most of the head domain, it lacks motor activity, but it retains all of Myo10’s other domains and is thus expected to retain the ability to bind to light chains of the calmodulin superfamily, to membranes containing PI(3,4,5)P3 or PI(4,5)P3, to microtubules, and to proteins that bind the Myo10 tail such as DCC, neogenin, and β-integrins. Headless Myo10 has been hypothesized to act as a scaffolding protein for its various binding partners and/or as a “natural” dominant negative that can inhibit the actions of full-length Myo10.[40][41]

Evolutionary relationships

A CAD cell (a neuronal cell line) expressing GFP-Myo10 (green) was stained for actin filaments (red) to visualize the slender cellular protrusions known as filopodia. Overexpressing Myo10 induces large numbers of filopodia and is responsible for the unusually large number of filopodia on this cell. (Image courtesy of Aurea D. Sousa and Richard E. Cheney)

Myo10 is a member of an evolutionarily ancient group of myosins whose tails contain MyTH4-FERM domains and that have been shown to have important functions in cellular protrusions based on actin bundles such as filopodia, microvilli, and inner ear stereocilia.[43][44] The slime mold Dictyostelium expresses a MyTH4-FERM myosin known as myosin-7 that has 2 MyTH4-FERM supramodules but no PH domains.[43] Myo10 appears to have originated from an ancestral myosin-7-like protein approximately a billion years ago by several changes including loss of 1 MyTH4-FERM supramodule and addition of 3 PH domains. A Myo10 gene is present in organisms ranging from filozoans and choanoflagellates (the protozoan groups most closely related to multicellular animals) to humans.[43] Myo10 was lost in the invertebrate lineages leading to organisms such as fruit flies and nematodes, although these lineages do express other MyTH4-FERM myosins such as myosin-7. Humans express 3 MyTH4-FERM myosins in addition to Myo10: MYO7A, the gene that is mutated in Usher syndrome 1b deaf-blindness; MYO7B, a component of an adhesion complex at the tips of microvilli; and MYO15A, a myosin that localizes to the tips of inner ear stereocilia and that is mutated in DFNB3 deafness.[44] The head domains of the other MyTH4-FERM myosins expressed in human exhibit at most 45% overall amino acid sequence identity with Myo10 and their tail domains each contain 2 MyTH4-FERM domains instead of the 3 PH domains and 1 MyTH4-FERM domain in Myo10.[8][44]

Cellular function

A striking property of Myo10 is that it localizes to the tips of filopodia, a property that most other myosins lack. When Myo10 was tagged with Green Fluorescent Protein (GFP) and expressed in cells, small puncta of GFP-Myo10 were observed moving forward within filopodia towards the tip at rates of ~100 nm/s.[45] Imaging with single-molecule sensitivity revealed similar movements of individual Myo10 dimers at rates of ~600-1400 nm/s.[46][47][48] GFP-Myo10 also moves rearward in filopodia at retrograde flow rates of ~15 nm/s. These observations led to the hypothesis that Myo10 molecules use their motor activity to move themselves rapidly forward along filopodial actin filaments and can bind to filopodial actin filaments to be carried slowly rearward by retrograde actin flow.[45] This “intrafilopodial motility” of Myo10 has led to suggestions that Myo10 functions as a motor protein for transporting cargos within filopodia. Myo10 also has important functions in the formation and/or stabilization of filopodia, with Myo10 overexpression increasing the number and length of filopodia, while knockdown or knockout of Myo10 decreases filopodia.[45][49][50] Myo10 also has important functions in cell division, particularly in mitotic spindle orientation.[33][51][52][53] Myo10 is also required to cluster the excess centrosomes that are a hallmark of cancer cells,[52] a process of great interest because cancer cells need to cluster their centrosomes to successfully divide.

Role in disease

Growing evidence demonstrates that Myo10 has important roles in cancer.[9] In addition to its role in clustering the excess centrosomes of cancer cells,[52] Myo10 is a key component of invadopodia, filopodia-related protrusions that cancer cells use to invade their surroundings.[54] Several microRNAs that suppress cancer cell invasion have also been reported to act in part by targeting the Myo10 mRNA.[55][56] Knockout or knockdown of Myo10 is reported to suppress cancer cell invasion or spread in experimental models of breast cancer,[14][15] lung cancer,[17] and glioma,[16] where knockout of Myo10 also increased the effectiveness of an otherwise ineffective chemotherapy agent. These results, plus research showing that knockout of Myo10 increased survival time by 260% in a mouse model of melanoma,[12] make Myo10 a potential anti-cancer target.

Model organisms

Several strains of Myo10 knockout mice have been generated and demonstrate that Myo10 has important functions during development in processes such as neural tube closure, eye development, blood vessel formation, and pigmentation.[11][12][13] Approximately ~50% of the mouse embryos that lacked Myo10 failed to close their neural tube during early brain development and exhibited the lethal birth defect of exencephaly.[11][13] Interestingly, knockout embryos that did not develop exencephaly were able to survive birth, grow to adulthood, and produce offspring, indicating that Myo10 is not essential for adult survival. The Myo10 knockout mice that survived birth had high frequencies of several abnormalities, with ~100% exhibiting a white belly spot, a pigmentation defect associated with decreased ability of the melanocytes and their precursors to migrate.[12] Myo10 KO mice also exhibited the eye defect known as persistent fetal vasculature (PFV) or persistent hyperplastic primary vitreous (PHPV), a condition resulting from the failure of the developing eye’s hyaloid vasculature to regress properly.[11][13] Myo10 KO mice also exhibited high frequencies of other eye abnormalities such as small eyes (microphthalmia) and lens defects. Myo10 KO mice also averaged ~20% lower in weight than control mice and approximately half had one or more webbed digits (syndactyly).[11][13]

A conditional knockout mouse line called Myo10tm2(KOMP)Wtsi was generated at the Wellcome Trust Sanger Institute.[57] Male and female animals underwent a standardized phenotypic screen[58] to determine the effects of deletion.[59][60][61][62] Additional screens performed: - In-depth immunological phenotyping[63]

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000145555Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000022272Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ "Entrez Gene: MYO10 myosin X".
  6. ^ Bement WM, Hasson T, Wirth JA, Cheney RE, Mooseker MS (July 1994). "Identification and overlapping expression of multiple unconventional myosin genes in vertebrate cell types". Proceedings of the National Academy of Sciences of the United States of America. 91 (14): 6549–53. Bibcode:1994PNAS...91.6549B. doi:10.1073/pnas.91.14.6549. PMC 44240. PMID 8022818.
  7. ^ Hasson T, Skowron JF, Gilbert DJ, Avraham KB, Perry WL, Bement WM, et al. (September 1996). "Mapping of unconventional myosins in mouse and human". Genomics. 36 (3): 431–9. doi:10.1006/geno.1996.0488. PMID 8884266.
  8. ^ a b c d e f g Berg JS, Derfler BH, Pennisi CM, Corey DP, Cheney RE (October 2000). "Myosin-X, a novel myosin with pleckstrin homology domains, associates with regions of dynamic actin". Journal of Cell Science. 113. 113 Pt 19: 3439–51. PMID 10984435.
  9. ^ a b c Courson DS, Cheney RE (May 2015). "Myosin-X and disease". Experimental Cell Research. 334 (1): 10–5. doi:10.1016/j.yexcr.2015.03.014. PMC 4433855. PMID 25819274.
  10. ^ Tokuo H (2020). "Myosin X". Advances in Experimental Medicine and Biology. 1239: 391–403. doi:10.1007/978-3-030-38062-5_17. ISBN 978-3-030-38061-8. PMID 32451868.
  11. ^ a b c d e Heimsath EG, Yim YI, Mustapha M, Hammer JA, Cheney RE (December 2017). "Myosin-X knockout is semi-lethal and demonstrates that myosin-X functions in neural tube closure, pigmentation, hyaloid vasculature regression, and filopodia formation". Scientific Reports. 7 (1): 17354. Bibcode:2017NatSR...717354H. doi:10.1038/s41598-017-17638-x. PMC 5725431. PMID 29229982.
  12. ^ a b c d e Tokuo H, Bhawan J, Coluccio LM (July 2018). "Myosin X is required for efficient melanoblast migration and melanoma initiation and metastasis". Scientific Reports. 8 (1): 10449. Bibcode:2018NatSR...810449T. doi:10.1038/s41598-018-28717-y. PMC 6041326. PMID 29993000.
  13. ^ a b c d e Bachg AC, Horsthemke M, Skryabin BV, Klasen T, Nagelmann N, Faber C, et al. (January 2019). "Phenotypic analysis of Myo10 knockout (Myo10tm2/tm2) mice lacking full-length (motorized) but not brain-specific headless myosin X". Scientific Reports. 9 (1): 597. Bibcode:2019NatSR...9..597B. doi:10.1038/s41598-018-37160-y. PMC 6345916. PMID 30679680.
  14. ^ a b Arjonen A, Kaukonen R, Mattila E, Rouhi P, Högnäs G, Sihto H, et al. (March 2014). "Mutant p53-associated myosin-X upregulation promotes breast cancer invasion and metastasis". The Journal of Clinical Investigation. 124 (3): 1069–82. doi:10.1172/JCI67280. PMC 3934176. PMID 24487586.
  15. ^ a b Cao R, Chen J, Zhang X, Zhai Y, Qing X, Xing W, et al. (July 2014). "Elevated expression of myosin X in tumours contributes to breast cancer aggressiveness and metastasis". British Journal of Cancer. 111 (3): 539–50. doi:10.1038/bjc.2014.298. PMC 4119973. PMID 24921915.
  16. ^ a b Kenchappa RS, Mistriotis P, Wisniewski E, Bhattacharya S, Kulkarni T, West R, et al. (December 2020). "Myosin 10 Regulates Invasion, Mitosis, and Metabolic Signaling in Glioblastoma". iScience. 23 (12): 101802. Bibcode:2020iSci...23j1802K. doi:10.1016/j.isci.2020.101802. PMC 7702012. PMID 33299973.
  17. ^ a b Summerbell ER, Mouw JK, Bell JS, Knippler CM, Pedro B, Arnst JL, et al. (July 2020). "Epigenetically heterogeneous tumor cells direct collective invasion through filopodia-driven fibronectin micropatterning". Science Advances. 6 (30): eaaz6197. Bibcode:2020SciA....6.6197S. doi:10.1126/sciadv.aaz6197. PMC 7439406. PMID 32832657.
  18. ^ Berg et al., 2000
  19. ^ Hirano et al., 2011
  20. ^ Lu et al., 2011
  21. ^ Wei et al., 2011
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Further reading

  • Overview of all the structural information available in the PDB for UniProt: Q9HD67 (Unconventional myosin-X) at the PDBe-KB.