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Long interspersed nuclear element

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(Redirected from LINEs)
ORF2 protein (exhibiting reverse transcriptase and endonuclease activity) from human LINE-1.
Genetic structure of murine LINE1 and SINEs. Bottom: proposed structure of L1 RNA-protein (RNP) complexes. ORF1 proteins form trimers, exhibiting RNA binding and nucleic acid chaperone activity.

Long interspersed nuclear elements (LINEs)[1] (also known as long interspersed nucleotide elements[2] or long interspersed elements[3]) are a group of non-LTR (long terminal repeat) retrotransposons that are widespread in the genome of many eukaryotes.[4][5] LINEs contain an internal Pol II promoter to initiate transcription into mRNA, and encode one or two proteins, ORF1 and ORF2.[6] The functional domains present within ORF1 vary greatly among LINEs, but often exhibit RNA/DNA binding activity. ORF2 is essential to successful retrotransposition, and encodes a protein with both reverse transcriptase and endonuclease activity.[7]

LINEs are the most abundant transposable element within the human genome,[8] with approximately 20.7% of the sequences identified as being derived from LINEs. The only active lineage of LINE found within humans belongs to the LINE-1 class, and is referred to as L1Hs.[9] The human genome contains an estimated 100,000 truncated and 4,000 full-length LINE-1 elements.[10] Due to the accumulation of random mutations, the sequence of many LINEs has degenerated to the extent that they are no longer transcribed or translated. Comparisons of LINE DNA sequences can be used to date transposon insertions in the genome.

History of discovery

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The first description of an approximately 6.4 kb long LINE-derived sequence was published by J. Adams et al. in 1980.[11]

Classification of LINEs

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Based on structural features and the phylogeny of the essential protein ORF2p, LINEs can be separated into six main groups, referred to as R2, RanI, L1, RTE, I and Jockey. These groups can further be subdivided into at least 28 clades.[12]

In plant genomes, so far only LINEs of the L1 and RTE clade have been reported.[13][14][15] Whereas L1 elements diversify into several subclades, RTE-type LINEs are highly conserved, often constituting a single family.[16][17]

In fungi, Tad, L1, CRE, Deceiver and Inkcap-like elements have been identified,[18] with Tad-like elements appearing exclusively in fungal genomes.[19]

All LINEs encode a least one protein, ORF2, which contains an RT and an endonuclease (EN) domain, either an N-terminal APE or a C-terminal RLE or rarely both. A ribonuclease H domain is occasionally present. Except for the evolutionary ancient R2 and RTE superfamilies, LINEs usually encode for another protein named ORF1, which may contain an Gag-knuckle, a L1-like RRM (InterPro: IPR035300), and/or an esterase. LINE elements are relatively rare compared to LTR-retrotransposons in plants, fungi or insects, but are dominant in vertebrates and especially in mammals, where they represent around 20% of the genome.[12]: fig. 1 

L1 elements

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Main article: LINE1

The LINE-1/L1-element is one of the elements that are still active in the human genome today. It is found in all therian mammals[20][21] except megabats.[22]

Other elements

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Remnants of L2 and L3 elements are found in the human genome.[23] It is estimated that L2 and L3 elements were active ~200-300 million years ago. Due to the age of L2 elements found within therian genomes, they lack flanking target site duplications.[24] The L2 (and L3) elements are in the same group as the CR1 clade, Jockey.[25]

Incidence

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In human

[edit]

In the first human genome draft the fraction of LINE elements of the human genome was given as 21% and their copy number as 850,000. Of these, L1, L2 and L3 elements made up 516,000, 315,000 and 37,000 copies, respectively. The non-autonomous SINE elements which depend on L1 elements for their proliferation make up 13% of the human genome and have a copy number of around 1.5 million.[23] They probably originated from the RTE family of LINEs.[26] Recent estimates show the typical human genome contains on average 100 L1 elements with potential for mobilization, however there is a fair amount of variation and some individuals may contain a larger number of active L1 elements, making these individuals more prone to L1-induced mutagenesis.[27]

Increased L1 copy numbers have also been found in the brains of people with schizophrenia, indicating that LINE elements may play a role in some neuronal diseases.[28]

Mechanism of target-primed reverse transcription (TPRT), directly at the site of integration: L1 RNP recognize AAAATT hexanucleotides and ORF2 endonuclease activity cleaves the DNA first-strand. L1 polyA tail associate with TTTT overhang and the host DNA is used as a primer to initiate reverse-transcription. ORF2 probably also mediate second-strand cleavage and attachment of newly synthesized cDNA to the DNA template, using again host DNA as a primer for second-strand synthesis.

Propagation

[edit]

LINE elements propagate by a so-called target primed reverse transcription mechanism (TPRT), which was first described for the R2 element from the silkworm Bombyx mori.

ORF2 (and ORF1 when present) proteins primarily associate in cis with their encoding mRNA, forming a ribonucleoprotein (RNP) complex, likely composed of two ORF2s and an unknown number of ORF1 trimers.[29] The complex is transported back into the nucleus, where the ORF2 endonuclease domain opens the DNA (at TTAAAA hexanucleotide motifs in mammals[30]). Thus, a 3'OH group is freed for the reverse transcriptase to prime reverse transcription of the LINE RNA transcript. Following the reverse transcription the target strand is cleaved and the newly created cDNA is integrated[31]

New insertions create short target site duplications (TSDs), and the majority of new inserts are severely 5’-truncated (average insert size of 900bp in humans) and often inverted (Szak et al., 2002). Because they lack their 5’UTR, most of new inserts are non functional.

Regulation of LINE activity

[edit]

It has been shown that host cells regulate L1 retrotransposition activity, for example through epigenetic silencing. For example, the RNA interference (RNAi) mechanism of small interfering RNAs derived from L1 sequences can cause suppression of L1 retrotransposition.[32]

In plant genomes, epigenetic modification of LINEs can lead to expression changes of nearby genes and even to phenotypic changes: In the oil palm genome, methylation of a Karma-type LINE underlies the somaclonal, 'mantled' variant of this plant, responsible for drastic yield loss.[33]

Human APOBEC3C mediated restriction of LINE-1 elements were reported and it is due to the interaction between A3C with the ORF1p that affects the reverse transcriptase activity.[34]

Association with disease

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A historic example of L1-conferred disease is Haemophilia A, which is caused by insertional mutagenesis.[35] There are nearly 100 examples of known diseases caused by retroelement insertions, including some types of cancer and neurological disorders.[36] Correlation between L1 mobilization and oncogenesis has been reported for epithelial cell cancer (carcinoma).[37] Hypomethylation of LINES is associated with chromosomal instability and altered gene expression[38] and is found in various cancer cell types in various tissues types.[39][38] Hypomethylation of a specific L1 located in the MET onco gene is associated with bladder cancer tumorogenesis,[40] Shift work sleep disorder[41] is associated with increased cancer risk because light exposure at night reduces melatonin, a hormone that has been shown to reduce L1-induced genome instability.[42]

References

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