ProblemRecurrent pregnancy loss (RPL) is the spontaneous loss of two or more consecutive pregnanc... more ProblemRecurrent pregnancy loss (RPL) is the spontaneous loss of two or more consecutive pregnancies prior to 20 weeks of gestation, occurring in 1% of the reproductive‐age population. It is a major cause of infertility in India with a staggering 7.46% prevalence rate.Method of studyBlood and product of conception (POCs) from RPL cases (n = 65) were enrolled for this study, along with cases of medically terminated pregnancy (MTP, n = 80) and term delivery cases (n = 90) as control. ELISA for progesterone and progesterone induced blocking factor (PIBF) levels was carried out, followed by mRNA expression analysis of progesterone receptor isoform B (PR‐B) and its downstream immunomodulatory effectors, namely, PIBF, IL‐10 and IL‐12. Screening of PROGINS haplotype of PR gene and PIBF polymorphism were also conducted to correlate with their respective gene expression profiles.ResultsSerum progesterone level was found to be comparable in the RPL and MTP cases. Although the mRNA expression ...
ProblemRecurrent pregnancy loss (RPL) is the spontaneous loss of two or more consecutive pregnanc... more ProblemRecurrent pregnancy loss (RPL) is the spontaneous loss of two or more consecutive pregnancies prior to 20 weeks of gestation, occurring in 1% of the reproductive‐age population. It is a major cause of infertility in India with a staggering 7.46% prevalence rate.Method of studyBlood and product of conception (POCs) from RPL cases (n = 65) were enrolled for this study, along with cases of medically terminated pregnancy (MTP, n = 80) and term delivery cases (n = 90) as control. ELISA for progesterone and progesterone induced blocking factor (PIBF) levels was carried out, followed by mRNA expression analysis of progesterone receptor isoform B (PR‐B) and its downstream immunomodulatory effectors, namely, PIBF, IL‐10 and IL‐12. Screening of PROGINS haplotype of PR gene and PIBF polymorphism were also conducted to correlate with their respective gene expression profiles.ResultsSerum progesterone level was found to be comparable in the RPL and MTP cases. Although the mRNA expression ...
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