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A Linkage Map of the Rat Genome Derived from Three F2 Crosses

  1. Marie Thérèse Bihoreau1,4,
  2. Dominique Gauguier1,
  3. Norihiro Kato1,
  4. Gina Hyne1,
  5. Klaus Lindpaintner2,
  6. John P. Rapp3,
  7. Michael R. James1, and
  8. G. Mark Lathrop1
  1. 1The Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, UK; 2Department of Cardiology, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts 02115; 3Department of Physiology and Molecular Medicine, Medical College of Ohio, Toledo, Ohio 43699-0008

Abstract

We report the construction of a dense linkage map of the rat genome integrating 767 simple sequence length polymorphism markers, combined over three crosses with high rates of polymorphism. F2 populations from WKY × S (n = 159), BN × S (n = 91), and BN × GK (n = 139) were selected and genotyped for combinations of microsatellite markers. The loci define 21 linkage groups corresponding to the 20 rat autosomal chromosomes and the X chromosome. The map spans a genetic length of 1998 cM. This combined linkage map should facilitate the advancement of genetic studies for a wide variety of rat models characterized for complex phenotypes.

Footnotes

  • ↵4 Corresponding author.

  • E-MAIL marie-therese.bihoreau{at}well.ox.ac.uk; FAX (44) 1865 742 187.

    • Received December 2, 1996.
    • Accepted March 24, 1997.