In the present paper, two colorimetry analytic methods-ELISA and OD260 methods were explored and compared to study the affinity changes in the SELEX for aptamer ...
Abstract—Affinity detection is a key step of the SELEX screening process for aptamer. Tradtional detection depends on the isotope.
The results showed that the affinity changed almost similarly in the two detecting ways. Comparatively, ELISA was a proper affinity detecting method. Although ...
May 17, 2017 · Bibliographic details on Affinity Changes of ssDNA Library in Vibrio SELEX by Two Detecting Methods.
Aptamers are widely used for the detection of bacteria with SELEX procedure where they show high specificity and binding affinity toward their targets (13, 16).
Affinity changes of ssDNA library in vibrio SELEX by two detecting methods ... Therefore, searching for a new, safe and proper detection for the affinity of ssDNA ...
Aptamers are selected by a combinatorial chemistry method known as SELEX (systematic evolution of ligands by exponential enrichment) from a library of nucleic ...
2000 – 2009 ; Jiang Zheng, ; Jiaxiang Li, Yubao Li, ; Ting Li: Affinity Changes of ssDNA Library in Vibrio SELEX by Two Detecting Methods. BMEI 2009: ...
Aug 17, 2023 · The selected aptamer was labelled with a fluorophore to determine its binding affinity with L. interrogans. The single-stranded DNA (ssDNA) ...
