Selcuk Ozdemir, M.Ed Raggio Research Center for STEM (Science, Technology, Engineering &Mathe... more Selcuk Ozdemir, M.Ed Raggio Research Center for STEM (Science, Technology, Engineering &Mathematics) Education University of Nevada, Reno United States selcuk@unr.edu ... Pamela Cantrell, Ph.D Director, Raggio Research Center for STEM (Science, Technology, ...
Deltamethrin is a widely used synthetic pyrethroid pesticide that protects agricultural yields, i... more Deltamethrin is a widely used synthetic pyrethroid pesticide that protects agricultural yields, including crops, fruits, and vegetables from insect-pests. It is known that deltamethrin toxication leads to metabolic disorders and has detrimental effects on the brain and liver in different organisms. However, the harmful effects of deltamethrin toxication on aquatic animals remain unclear. In the present study, we aimed to evaluate the adverse effects of deltamethrin toxication by performing a histopathological examination, an immunofluorescence assay, and a qRT-PCR on common carp. We observed that a low-dose (0.04μM) and a high-dose (0.08μM) of deltamethrin exposure caused lamellar cells hyperplasia and inflammatory cells infiltration in the gills, hyperemia, diffuse hydropic degenerations and focal necrosis in the hepatocytes, necrotic changes in the neurons, and also induced activation of inducible Nitric Oxide Synthase (iNOS) and 8-hydroxy-2-deoxyguanosine (8-OHdG) in the gills, l...
Cypermethrin (Cyp), a known neurotoxic pesticide, is widely used in agricultural applications. In... more Cypermethrin (Cyp), a known neurotoxic pesticide, is widely used in agricultural applications. In the present study, the aim was to determine the histopathological effects of Cyp toxication and evaluate the activation of inducible nitric oxide synthetase (iNOS) and 8-hydroxy-2-deoxyguanosine (8-OHdG) using an immunofluorescence assay. Thereafter, we identified the expressions of caspase 3, capsase 8, iNOS, and metallothionein 1 (MT1) genes in common carp using quantitative reverse transcription polymerase chain reaction (qRT-PCR). High and low doses of Cyp were administered to experimental groups for 24, 48, 72, and 96 h. As a result, necrotic neurons in different stages and desquamation of ependymal cells due to necrosis were detected in the brain. Histopathological changes, including hyperplasia of lamellar cells, telangiectasia of lamellae and thickening due to cellular infiltration in gills, hemorrhage, diffuse hydropic degeneration, and focal necrosis in the liver were observed...
In this study, we aimed to identify the toxic effects of chlorpyrifos exposure on the tissues of ... more In this study, we aimed to identify the toxic effects of chlorpyrifos exposure on the tissues of common carp. For this purpose, we evaluated histopathological changes in the brain, gills, liver, kidney, testis, and ovaries after 21 days of chlorpyrifos exposure. Activation of 8-OHdG, cleaved caspase-3, and iNOS were assesed by immunofluorescence assay in chlorpyrifos-exposed brain and liver tissue. Additionally, we measured the expression levels of caspase-3, caspase-8, iNOS, MT1, CYP1A, and CYP3A genes in chlorpyrifos-exposed brain tissue, as well as the expression levels of FSH and LH genes in chlorpyrifos-exposed ovaries, using qRT-PCR. We observed severe histopathological lesions, including inflammation, degeneration, necrosis, and hemorrhage, in the evaluated tissues of common carp after both high and low levels of exposure to chlorpyrifos. We detected strong and diffuse signs of immunofluorescence reaction for 8-OHdG, iNOS, and cleaved caspase-3 in the chlorpyrifos-exposed bra...
İklim türleri konusunda kaynaklar tarandığında iki iklim türüne önem verildiği görülmektedir: açı... more İklim türleri konusunda kaynaklar tarandığında iki iklim türüne önem verildiği görülmektedir: açık ve kapalı iklim tipi. Ancak bu bölümde örgütsel iklim tipleri olarak açık, bagımsız, kontrollü, samimi, babacan ve kapalı olmak üzere altı tip olarak değişebilir.
Selcuk Ozdemir, M.Ed Raggio Research Center for STEM (Science, Technology, Engineering &Mathe... more Selcuk Ozdemir, M.Ed Raggio Research Center for STEM (Science, Technology, Engineering &Mathematics) Education University of Nevada, Reno United States selcuk@unr.edu ... Pamela Cantrell, Ph.D Director, Raggio Research Center for STEM (Science, Technology, ...
Deltamethrin is a widely used synthetic pyrethroid pesticide that protects agricultural yields, i... more Deltamethrin is a widely used synthetic pyrethroid pesticide that protects agricultural yields, including crops, fruits, and vegetables from insect-pests. It is known that deltamethrin toxication leads to metabolic disorders and has detrimental effects on the brain and liver in different organisms. However, the harmful effects of deltamethrin toxication on aquatic animals remain unclear. In the present study, we aimed to evaluate the adverse effects of deltamethrin toxication by performing a histopathological examination, an immunofluorescence assay, and a qRT-PCR on common carp. We observed that a low-dose (0.04μM) and a high-dose (0.08μM) of deltamethrin exposure caused lamellar cells hyperplasia and inflammatory cells infiltration in the gills, hyperemia, diffuse hydropic degenerations and focal necrosis in the hepatocytes, necrotic changes in the neurons, and also induced activation of inducible Nitric Oxide Synthase (iNOS) and 8-hydroxy-2-deoxyguanosine (8-OHdG) in the gills, l...
Cypermethrin (Cyp), a known neurotoxic pesticide, is widely used in agricultural applications. In... more Cypermethrin (Cyp), a known neurotoxic pesticide, is widely used in agricultural applications. In the present study, the aim was to determine the histopathological effects of Cyp toxication and evaluate the activation of inducible nitric oxide synthetase (iNOS) and 8-hydroxy-2-deoxyguanosine (8-OHdG) using an immunofluorescence assay. Thereafter, we identified the expressions of caspase 3, capsase 8, iNOS, and metallothionein 1 (MT1) genes in common carp using quantitative reverse transcription polymerase chain reaction (qRT-PCR). High and low doses of Cyp were administered to experimental groups for 24, 48, 72, and 96 h. As a result, necrotic neurons in different stages and desquamation of ependymal cells due to necrosis were detected in the brain. Histopathological changes, including hyperplasia of lamellar cells, telangiectasia of lamellae and thickening due to cellular infiltration in gills, hemorrhage, diffuse hydropic degeneration, and focal necrosis in the liver were observed...
In this study, we aimed to identify the toxic effects of chlorpyrifos exposure on the tissues of ... more In this study, we aimed to identify the toxic effects of chlorpyrifos exposure on the tissues of common carp. For this purpose, we evaluated histopathological changes in the brain, gills, liver, kidney, testis, and ovaries after 21 days of chlorpyrifos exposure. Activation of 8-OHdG, cleaved caspase-3, and iNOS were assesed by immunofluorescence assay in chlorpyrifos-exposed brain and liver tissue. Additionally, we measured the expression levels of caspase-3, caspase-8, iNOS, MT1, CYP1A, and CYP3A genes in chlorpyrifos-exposed brain tissue, as well as the expression levels of FSH and LH genes in chlorpyrifos-exposed ovaries, using qRT-PCR. We observed severe histopathological lesions, including inflammation, degeneration, necrosis, and hemorrhage, in the evaluated tissues of common carp after both high and low levels of exposure to chlorpyrifos. We detected strong and diffuse signs of immunofluorescence reaction for 8-OHdG, iNOS, and cleaved caspase-3 in the chlorpyrifos-exposed bra...
İklim türleri konusunda kaynaklar tarandığında iki iklim türüne önem verildiği görülmektedir: açı... more İklim türleri konusunda kaynaklar tarandığında iki iklim türüne önem verildiği görülmektedir: açık ve kapalı iklim tipi. Ancak bu bölümde örgütsel iklim tipleri olarak açık, bagımsız, kontrollü, samimi, babacan ve kapalı olmak üzere altı tip olarak değişebilir.
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