International Journal of Medicinal Mushrooms, 2023
The genus Ganoderma has a long history of use in traditional Asiatic medicine due to its differen... more The genus Ganoderma has a long history of use in traditional Asiatic medicine due to its different nutritional and medicinal properties. In Mexico, the species G. tuberculosum is used in indigenous communities, for example, the Wixaritari and mestizos of Villa Guerrero Jalisco for the treatment of diseases that may be related to parasitic infections; however, few chemical studies corroborate its traditional medicinal potential. Thereby, the objective of this study was to isolate and identify anti-parasitic activity compounds from a strain of G. tuberculosum native to Mexico. From the fruiting bodies of G. tuberculosum (GVL-21) a hexane extract was obtained which was subjected to guided fractioning to isolate pure compounds. The in vitro anti-parasitic activity of the pure compound (IC50) was assayed against Leishmania amazonensis, Trypanosoma cruzi, Acanthamoeba castellanii Neff, and Naegleria fowleri. Furthermore, the cytotoxicity (CC50) of the isolated compounds was determined against murine macrophages. The guided fractioning produced 5 compounds: ergosterol (1), ergosta-4,6,8(14),22-tetraen-3-one (2), ergosta-7,22-dien-3β-ol (3), 3,5-dihydroxy-ergosta-7,22-dien-6-one (4), and ganoderic acid DM (5). Compounds 2 and 5 showed the best anti-parasitic activity in an IC50 range of 54.34 ± 8.02 to 12.38 ± 2.72 µM against all the parasites assayed and low cytotoxicity against murine macrophages. The present study showed for the first time the in vitro anti-parasitic activity of compounds 1-5 against L. amazonensis, T. cruzi, A. castellanii Neff, and N. fowleri, corroborating the medicinal potential of Ganoderma and its traditional applications.
A red pigment produced by a Mexican isolate of Cercospora piaropi (waterhyacinth pathogen) has be... more A red pigment produced by a Mexican isolate of Cercospora piaropi (waterhyacinth pathogen) has been isolated and identified as cercosporin. The kinetic of cercosporin production in culture media during dark/light regimes was evaluated. When C. piaropi was cultivated in continuous light and potato dextrose broth culture, a maximum of cercosporin production was observed (72.59 mg/l). Despite other reports, C piaropi Mexican isolate produce cercosporin in dark conditions (25.70 mg/l). The results suggest that production of cercosporin in C. piaropi-waterhyacinth pathogenesis is an important factor to take into account in biocontrol strategies.
Asparagus cultivation is highly profitable and is appreciated in international gourmet cuisine. I... more Asparagus cultivation is highly profitable and is appreciated in international gourmet cuisine. In Mexico, the production of this vegetable is mainly for export, in the state of Veracruz it has been recently introduced. The objective of the research was to characterize by morphology, molecular techniques and pathogenicity test the Fusarium species isolated from symptomatic tissues of asparagus shoot. A directed sampling was carried out on plants that showed rot, wilting and dark brown spots. Fungal isolates were obtained from diseased shoots, which were disinfected and seeded on potato dextrose agar. Isolates were purified in monosporic cultures by hyphal tip. Four species of Fusarium were identified by taxonomic morphology, in a selective carnation leaf agar medium, then pathogenicity tests were carried out, complying with Koch's postulates. Next, the strains that were pathogenic were characterized by molecular techniques, and their identity as Fusarium oxysporum and Fusarium p...
Additional file 1: Table S1. Clonal complexes defined by MIRU-VNTR of M. tuberculosis strains fro... more Additional file 1: Table S1. Clonal complexes defined by MIRU-VNTR of M. tuberculosis strains from Veracruz city, México.
Background: Cervical cancer is a major public health issue worldwide, occurring in the vast major... more Background: Cervical cancer is a major public health issue worldwide, occurring in the vast majority of cases (85%) in low-income countries. Human papillomavirus (HPV) mainly infects the mucosal epithelium, and a small portion causes over 600,000 cases every year worldwide at various anatomical spots, mainly leading to anogenital and head and neck. Introduction: The E6 oncoprotein encoded by cancer-associated alpha HPV can transform epithelial cells into tumorigenic tissue. Therapy for this infection and blocking of the HPV E6 oncoprotein could be provided with cost-effective and abundant natural products which are an exponentially growing topic in the literature. Finding an active natural compound that readily blocks HPV E6 oncoprotein which could be available for developing countries without expensive extraction processes or costly synthetic pathways is of major interest. Method: Molecular dynamics simulations was performed using the most up-to-date AMBER protein force field ff14S...
International Journal of Medicinal Mushrooms, 2023
The genus Ganoderma has a long history of use in traditional Asiatic medicine due to its differen... more The genus Ganoderma has a long history of use in traditional Asiatic medicine due to its different nutritional and medicinal properties. In Mexico, the species G. tuberculosum is used in indigenous communities, for example, the Wixaritari and mestizos of Villa Guerrero Jalisco for the treatment of diseases that may be related to parasitic infections; however, few chemical studies corroborate its traditional medicinal potential. Thereby, the objective of this study was to isolate and identify anti-parasitic activity compounds from a strain of G. tuberculosum native to Mexico. From the fruiting bodies of G. tuberculosum (GVL-21) a hexane extract was obtained which was subjected to guided fractioning to isolate pure compounds. The in vitro anti-parasitic activity of the pure compound (IC50) was assayed against Leishmania amazonensis, Trypanosoma cruzi, Acanthamoeba castellanii Neff, and Naegleria fowleri. Furthermore, the cytotoxicity (CC50) of the isolated compounds was determined against murine macrophages. The guided fractioning produced 5 compounds: ergosterol (1), ergosta-4,6,8(14),22-tetraen-3-one (2), ergosta-7,22-dien-3β-ol (3), 3,5-dihydroxy-ergosta-7,22-dien-6-one (4), and ganoderic acid DM (5). Compounds 2 and 5 showed the best anti-parasitic activity in an IC50 range of 54.34 ± 8.02 to 12.38 ± 2.72 µM against all the parasites assayed and low cytotoxicity against murine macrophages. The present study showed for the first time the in vitro anti-parasitic activity of compounds 1-5 against L. amazonensis, T. cruzi, A. castellanii Neff, and N. fowleri, corroborating the medicinal potential of Ganoderma and its traditional applications.
A red pigment produced by a Mexican isolate of Cercospora piaropi (waterhyacinth pathogen) has be... more A red pigment produced by a Mexican isolate of Cercospora piaropi (waterhyacinth pathogen) has been isolated and identified as cercosporin. The kinetic of cercosporin production in culture media during dark/light regimes was evaluated. When C. piaropi was cultivated in continuous light and potato dextrose broth culture, a maximum of cercosporin production was observed (72.59 mg/l). Despite other reports, C piaropi Mexican isolate produce cercosporin in dark conditions (25.70 mg/l). The results suggest that production of cercosporin in C. piaropi-waterhyacinth pathogenesis is an important factor to take into account in biocontrol strategies.
Asparagus cultivation is highly profitable and is appreciated in international gourmet cuisine. I... more Asparagus cultivation is highly profitable and is appreciated in international gourmet cuisine. In Mexico, the production of this vegetable is mainly for export, in the state of Veracruz it has been recently introduced. The objective of the research was to characterize by morphology, molecular techniques and pathogenicity test the Fusarium species isolated from symptomatic tissues of asparagus shoot. A directed sampling was carried out on plants that showed rot, wilting and dark brown spots. Fungal isolates were obtained from diseased shoots, which were disinfected and seeded on potato dextrose agar. Isolates were purified in monosporic cultures by hyphal tip. Four species of Fusarium were identified by taxonomic morphology, in a selective carnation leaf agar medium, then pathogenicity tests were carried out, complying with Koch's postulates. Next, the strains that were pathogenic were characterized by molecular techniques, and their identity as Fusarium oxysporum and Fusarium p...
Additional file 1: Table S1. Clonal complexes defined by MIRU-VNTR of M. tuberculosis strains fro... more Additional file 1: Table S1. Clonal complexes defined by MIRU-VNTR of M. tuberculosis strains from Veracruz city, México.
Background: Cervical cancer is a major public health issue worldwide, occurring in the vast major... more Background: Cervical cancer is a major public health issue worldwide, occurring in the vast majority of cases (85%) in low-income countries. Human papillomavirus (HPV) mainly infects the mucosal epithelium, and a small portion causes over 600,000 cases every year worldwide at various anatomical spots, mainly leading to anogenital and head and neck. Introduction: The E6 oncoprotein encoded by cancer-associated alpha HPV can transform epithelial cells into tumorigenic tissue. Therapy for this infection and blocking of the HPV E6 oncoprotein could be provided with cost-effective and abundant natural products which are an exponentially growing topic in the literature. Finding an active natural compound that readily blocks HPV E6 oncoprotein which could be available for developing countries without expensive extraction processes or costly synthetic pathways is of major interest. Method: Molecular dynamics simulations was performed using the most up-to-date AMBER protein force field ff14S...
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