A sample of Betula nana from the Linie reserve near Dąbrowa Chelminska, have been fingerprinted u... more A sample of Betula nana from the Linie reserve near Dąbrowa Chelminska, have been fingerprinted using random amplified polymorphic DNA (RAPD). The high level of genetic variation was detected. All individuals had unique genotypes, supporting the generally high resolving power of RAPD’s. For the conservation strategy, information about the distribution of the genetic variation within and among populations plays very important role. Thus, extensive study in other populations of dwarf birch is needed. Additional key words: genetic resources, conservation, population genetics. Addresses: A. Dzialuk, Kazimierz Wielki University, Institute of Biology and Environmental Protection, Department of Genetics, Chodkiewicza 30, 85-064 Bydgoszcz, Poland, e-mail: dzialuk@ukw.edu.pl G. Dąbrowska, O. Burnicka, A. Goc, N. Copernicus University, Laboratory of Genetics, Gagarina 9, 87-100 Torun, Poland W. Gugnacka-Fiedor, N. Copernicus University, Department of Plant Taxonomy and Geography, Gagarina 9, ...
Chronic inflammation is a well-recognised tumour-enabling component, which includes bioactive mol... more Chronic inflammation is a well-recognised tumour-enabling component, which includes bioactive molecules from cells infiltrating the tumour microenvironment and increases the risk of cancer progression. Since long-term use of the currently available anti-inflammatory drugs used in cancer therapy causes numerous side effects, the aim of this study was to investigate the effect of an extract isolated from the Coriolus versicolor fungus (CV extract) on HUVEC endothelial cells and MCF-7 breast cancer cells in a pro-inflammatory microenvironment mimicked by lipopolysaccharide (LPS). The cells were simultaneously stimulated with the LPS and CV extract. After co-treatment, the cell viability, generation of reactive oxygen species (ROS), wound-healing assay, production of the pro-inflammatory and pro-angiogenic factors (interleukin (IL) 6, IL-8, and metalloproteinase (MMP) 9)), as well as expression of Toll-like receptor (TLR) 4 and phosphorylated IκB (p-IκB) were evaluated. The results show...
Glutathione is one of the most important and potent antioxidants. The development of pharmacologi... more Glutathione is one of the most important and potent antioxidants. The development of pharmacological compounds that can either increase or decrease glutathione concentrations has allowed investigation into the role of glutathione in various biological processes, including immune responses. Recent findings have shown that glutathione not only affects certain factors involved in immunological processes but also modifies complex immune reactions such as fever. Until recently, it was not known why some patients do not develop fever during infection. Data suggest that fever induction is associated with oxidative stress; therefore, antioxidants such as glutathione can reduce pyrexia. Surprisingly, new studies have shown that low glutathione levels can also inhibit fever. In this review, we focus on recent advances in this area, with an emphasis on the role of glutathione in immune responses accompanied by fever. We describe evidence showing that disturbed glutathione homeostasis may be re...
A sample of Betula nana from the Linie reserve near Dąbrowa Chelminska, have been fingerprinted u... more A sample of Betula nana from the Linie reserve near Dąbrowa Chelminska, have been fingerprinted using random amplified polymorphic DNA (RAPD). The high level of genetic variation was detected. All individuals had unique genotypes, supporting the generally high resolving power of RAPD’s. For the conservation strategy, information about the distribution of the genetic variation within and among populations plays very important role. Thus, extensive study in other populations of dwarf birch is needed. Additional key words: genetic resources, conservation, population genetics. Addresses: A. Dzialuk, Kazimierz Wielki University, Institute of Biology and Environmental Protection, Department of Genetics, Chodkiewicza 30, 85-064 Bydgoszcz, Poland, e-mail: dzialuk@ukw.edu.pl G. Dąbrowska, O. Burnicka, A. Goc, N. Copernicus University, Laboratory of Genetics, Gagarina 9, 87-100 Torun, Poland W. Gugnacka-Fiedor, N. Copernicus University, Department of Plant Taxonomy and Geography, Gagarina 9, ...
Chronic inflammation is a well-recognised tumour-enabling component, which includes bioactive mol... more Chronic inflammation is a well-recognised tumour-enabling component, which includes bioactive molecules from cells infiltrating the tumour microenvironment and increases the risk of cancer progression. Since long-term use of the currently available anti-inflammatory drugs used in cancer therapy causes numerous side effects, the aim of this study was to investigate the effect of an extract isolated from the Coriolus versicolor fungus (CV extract) on HUVEC endothelial cells and MCF-7 breast cancer cells in a pro-inflammatory microenvironment mimicked by lipopolysaccharide (LPS). The cells were simultaneously stimulated with the LPS and CV extract. After co-treatment, the cell viability, generation of reactive oxygen species (ROS), wound-healing assay, production of the pro-inflammatory and pro-angiogenic factors (interleukin (IL) 6, IL-8, and metalloproteinase (MMP) 9)), as well as expression of Toll-like receptor (TLR) 4 and phosphorylated IκB (p-IκB) were evaluated. The results show...
Glutathione is one of the most important and potent antioxidants. The development of pharmacologi... more Glutathione is one of the most important and potent antioxidants. The development of pharmacological compounds that can either increase or decrease glutathione concentrations has allowed investigation into the role of glutathione in various biological processes, including immune responses. Recent findings have shown that glutathione not only affects certain factors involved in immunological processes but also modifies complex immune reactions such as fever. Until recently, it was not known why some patients do not develop fever during infection. Data suggest that fever induction is associated with oxidative stress; therefore, antioxidants such as glutathione can reduce pyrexia. Surprisingly, new studies have shown that low glutathione levels can also inhibit fever. In this review, we focus on recent advances in this area, with an emphasis on the role of glutathione in immune responses accompanied by fever. We describe evidence showing that disturbed glutathione homeostasis may be re...
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