Grass pea (Lathyrus sativus L.) is a legume crop known to be an excellent source of protein, tole... more Grass pea (Lathyrus sativus L.) is a legume crop known to be an excellent source of protein, tolerant to drought, waterlogging, and salinity. The crop is used as an alternative source of protein to reduce malnutrition for resource-poor people and farmers leaving in marginal areas. However, due to the presence of a neurotoxin that causes lathyrism in the crop, it has been neglected and underutilized. As a necessary first step towards, therefore, this investigation was undertaken to assess the genetic diversity and population structure that existed within grass pea accessions collected from NorthWest Ethiopia using simple sequence repeat markers. Twenty-five grass pea accessions collected from the Ethiopian Biodiversity Institute were planted at the College of Agricultural Science, Ebonyi State University, Nigeria. The genomic DNA was extracted using Quick-DNA TM ZR Plant/Seed Miniprep Kit and amplified in an ABI Veriti PCR machine with 10 pairs of SSR markers in IITA, Ibadan, Nigeria. Out of 10 SSR primers, only
Amochi (Arisaema schimperianum Schott) is an off-season crop plant in southern Ethiopia, grown du... more Amochi (Arisaema schimperianum Schott) is an off-season crop plant in southern Ethiopia, grown during the dry season on residual moisture, for its edible tubers. It has gained importance as a "security crop" especially during the years of moisture stress and food shortage. Amochi is irritating in contact to the skin. Removal of this effect is an important question for breeding. As the first step, however we attempt to establish base line information of its breeding system and genetic variability using AFLPs. The extent of genetic differentiation among 11 populations (96 individuals) of amochi sampled along altitudinal gradients that varied from 1700 to 3200 m a.s.l. was investigated. The populations were classified in to three altitudinal groups: lowland (1700 to 2200 m a.s.l.), central-highland (2201 to 2600 m a.s.l.) and highland (2601 to 3200 m a.s.l.). Polymorphic loci (167) scored from four primer pair combinations, were used for principal component analysis (PCA), and analysis of molecular variance (AMOVA). Both PCA and unweighed pair group with arithmetic mean (UPGMA) clearly differentiated populations into their respective altitude groups, with large genetic distances. AMOVA analysis revealed 70.5%, 16.7% and 12.8% variability between altitude groups, between populations and within populations respectively. Average diversity indices within populations were also low. Since the largest proportion of variation is located between altitude groups, rather than within populations, we suggest future studies on the chemical composition, low irritation, and other desirable traits should consider populations from different altitude ranges.
Abstract Germination requirements of Typha latifolia, Iris pseudacorus and Impatiens glandulifera... more Abstract Germination requirements of Typha latifolia, Iris pseudacorus and Impatiens glandulifera were investigated under laboratory conditions. A light sensitive stimulus was demonstrated for Typha latifolia, but no evidence of phytochrome mediation was observed ...
The viability of Norway maple seeds collected 21 d before mass maturity (68%moisture content, wet... more The viability of Norway maple seeds collected 21 d before mass maturity (68%moisture content, wet basis) and at mass maturity (56% moisture content) was reduced from 52–85% to 0–7% if dried rapidly (at 10–12% r.h. and 15–17°C for 3 d, then 3 d over silica gel) to 4–5% moisture content. Moist storage of the fruits at 15°C improved the ability of the seeds to tolerate rapid desiccation considerably: 10 and 21 d of moist storage enabled seeds collected at mass maturity or 21 d earlier, respectively, to attain maximum desiccation tolerance to 4–5% moisture content. Moist storage and/or subsequent desiccation affected stachyose, sucrose, and to a lesser extent raffinose, concentrations. The oligosaccharide:total sugar ratio showed a similar pattern in relation to ability to germinate after desiccation to 4–5% moisture content among seeds collected on both dates: desiccation tolerance developed from nil to maximal in these seed populations between threshold oligosaccharide:total sugar val...
Grass pea (Lathyrus sativus L.) is a legume crop known to be an excellent source of protein, tole... more Grass pea (Lathyrus sativus L.) is a legume crop known to be an excellent source of protein, tolerant to drought, waterlogging, and salinity. The crop is used as an alternative source of protein to reduce malnutrition for resource-poor people and farmers leaving in marginal areas. However, due to the presence of a neurotoxin that causes lathyrism in the crop, it has been neglected and underutilized. As a necessary first step towards, therefore, this investigation was undertaken to assess the genetic diversity and population structure that existed within grass pea accessions collected from NorthWest Ethiopia using simple sequence repeat markers. Twenty-five grass pea accessions collected from the Ethiopian Biodiversity Institute were planted at the College of Agricultural Science, Ebonyi State University, Nigeria. The genomic DNA was extracted using Quick-DNA TM ZR Plant/Seed Miniprep Kit and amplified in an ABI Veriti PCR machine with 10 pairs of SSR markers in IITA, Ibadan, Nigeria. Out of 10 SSR primers, only
Amochi (Arisaema schimperianum Schott) is an off-season crop plant in southern Ethiopia, grown du... more Amochi (Arisaema schimperianum Schott) is an off-season crop plant in southern Ethiopia, grown during the dry season on residual moisture, for its edible tubers. It has gained importance as a "security crop" especially during the years of moisture stress and food shortage. Amochi is irritating in contact to the skin. Removal of this effect is an important question for breeding. As the first step, however we attempt to establish base line information of its breeding system and genetic variability using AFLPs. The extent of genetic differentiation among 11 populations (96 individuals) of amochi sampled along altitudinal gradients that varied from 1700 to 3200 m a.s.l. was investigated. The populations were classified in to three altitudinal groups: lowland (1700 to 2200 m a.s.l.), central-highland (2201 to 2600 m a.s.l.) and highland (2601 to 3200 m a.s.l.). Polymorphic loci (167) scored from four primer pair combinations, were used for principal component analysis (PCA), and analysis of molecular variance (AMOVA). Both PCA and unweighed pair group with arithmetic mean (UPGMA) clearly differentiated populations into their respective altitude groups, with large genetic distances. AMOVA analysis revealed 70.5%, 16.7% and 12.8% variability between altitude groups, between populations and within populations respectively. Average diversity indices within populations were also low. Since the largest proportion of variation is located between altitude groups, rather than within populations, we suggest future studies on the chemical composition, low irritation, and other desirable traits should consider populations from different altitude ranges.
Abstract Germination requirements of Typha latifolia, Iris pseudacorus and Impatiens glandulifera... more Abstract Germination requirements of Typha latifolia, Iris pseudacorus and Impatiens glandulifera were investigated under laboratory conditions. A light sensitive stimulus was demonstrated for Typha latifolia, but no evidence of phytochrome mediation was observed ...
The viability of Norway maple seeds collected 21 d before mass maturity (68%moisture content, wet... more The viability of Norway maple seeds collected 21 d before mass maturity (68%moisture content, wet basis) and at mass maturity (56% moisture content) was reduced from 52–85% to 0–7% if dried rapidly (at 10–12% r.h. and 15–17°C for 3 d, then 3 d over silica gel) to 4–5% moisture content. Moist storage of the fruits at 15°C improved the ability of the seeds to tolerate rapid desiccation considerably: 10 and 21 d of moist storage enabled seeds collected at mass maturity or 21 d earlier, respectively, to attain maximum desiccation tolerance to 4–5% moisture content. Moist storage and/or subsequent desiccation affected stachyose, sucrose, and to a lesser extent raffinose, concentrations. The oligosaccharide:total sugar ratio showed a similar pattern in relation to ability to germinate after desiccation to 4–5% moisture content among seeds collected on both dates: desiccation tolerance developed from nil to maximal in these seed populations between threshold oligosaccharide:total sugar val...
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