I have Ph.D. degree in cytology and genetics. I am interested in biodiversity, population genetics, karyotype, chromosome aberrations, molecular approaches , conservation strategies for wild plants
Toxin-contaminated foods and beverages are a major source of illness, may cause death, and have a... more Toxin-contaminated foods and beverages are a major source of illness, may cause death, and have a significant negative economic impact worldwide. Aflatoxin B1 (AFB1) is a potent toxin that may induce cancer after chronic low-level exposure. This study developed a quantitative recombinant AflR gene antiserum ELISA technique for aflatoxin B1 detection in contaminated food products. Aflatoxin B1 residuals from 36 food samples were analyzed with HPLC and VICAM. DNA was extracted from aflatoxin-contaminated samples and the AflR gene amplified using PCR. PCR products were purified and ligated into the pGEM-T vector. Recombinant plasmids were sequenced and transformed into competent E. coli (BL21). Molecular size and B-cell epitope prediction for the recombinant protein were assessed. The purified protein was used to induce the production of IgG antibodies in rabbits. Serum IgG was purified and labeled with alkaline phosphatase. Finally, indirect-ELISA was used to test the effectiveness of...
DNA barcodes have been considered as a tool to facilitate species identification based on their s... more DNA barcodes have been considered as a tool to facilitate species identification based on their simplicity and high-level accuracy compression to the complexity and subjective biases linked to morphological identification of taxa. MaturaseK gene “MatK” of the chloroplast is very crucial in the plant system which is involved in the group II intron splicing. The main objective of this current study is determining the relative utility of the “MatK” chloroplast gene for barcoding in fifteen legume trees by both single region and multiregional approaches. The chloroplast “MatK” gene sequences were submitted to GenBank and accession numbers (GenBank: LC602060, LC602154, LC602263, LC603347, LC603655, LC603845, LC603846, LC603847, LC604717, LC604718, LC605994, LC604799, LC605995, LC606468, LC606469) were obtained with sequence length ranging from 730 to 1545 nucleotides. These DNA sequences were aligned with database sequence using PROMALS server, Clustal Omega server and Bioedit program. A...
Quinoa (Chenopodium quinoa Willd.) is a halophytic crop that can withstand a variety of abiotic s... more Quinoa (Chenopodium quinoa Willd.) is a halophytic crop that can withstand a variety of abiotic stresses, including salt. The present research examined the mechanisms of salt tolerance in five different quinoa genotypes at four different salinity levels (control (60), 80, 120, and 160 mM NaCl). ISSR and SCoT analysis revealed high polymorphism percentages of 90.91% and 85.26%, respectively. Furthermore, ISSR 1 and SCoT 7 attained the greatest number of polymorphic amplicons (27 and 26), respectively. Notably, LINE-6 and M-28 genotypes demonstrated the greatest number of unique positive and negative amplicons (50 and 42) generated from ISSR and SCoT, respectively. Protein pattern analysis detected 11 bands with a polymorphism percentage 27.27% among the quinoa genotypes, with three unique bands distinguishable for the M-28 genotype. Similarity correlation indicated that the highest similarity was between S-10 and Regeolone-3 (0.657), while the lowest similarity was between M-28 and L...
Cyanobacteria comprise a good natural resource of a potential variety of neuro-chemicals, includi... more Cyanobacteria comprise a good natural resource of a potential variety of neuro-chemicals, including acetylcholinesterase inhibitors essential for Alzheimer’s disease treatment. Accordingly, eight different cyanobacterial species were isolated, identified, and evaluated on their growth on different standard nutrient media. It was found that the modified Navicula medium supported the highest growth of the test cyanobacteria. The effects of methylene chloride/methanol crude extracts of the test cyanobacteria on acetylcholinesterase activity were examined and compared. Anabaena variabilis (KU696637.1) crude extract recorded the highest acetylcholinesterase inhibition (62 ± 1.3%). Navicula medium chemical components were optimized through a Plackett–Burman factorial design. The biomass of Anabaena variabilis increased significantly when grown on the optimized medium compared to that of control. The chemical analysis of the fractions derived from Anabaena variabilis showed the presence of...
The risk of using synthetic insecticides to the environment, human health, and the emergence of n... more The risk of using synthetic insecticides to the environment, human health, and the emergence of new genera of pests resistant to that kind of drugs, have led to attention in natural compounds. The present study aimed at evaluating the insecticidal activity of 0.25–6 mg/cm2 of basil (Ocimum basilicum), black seeds (Nigella sativa), and lavender (Lavandula angustifolia) essential oils (EOs) against one of the major stored product pests, Sitophilus oryzae (L.). This was done by assessing mortality and repellent percentage assay in the adult stage, as well as analysing up and down-regulated genes associated with toxicity effect of selected EOs. The three studied EOs showed a toxic effect on S. oryzae; where O. basilicum and L. angustifolia EOs explicated 100% mortality at 6 mg/cm2 after 48 and 24 h, respectively. The highest repellence activity was recorded for O. basilicum EO at 0.75 mg/cm2 with value 82.3% after exposure time 5 h. In the highest dose (6 mg/cm2), the maximum up-regulat...
Tomato (Lycopersicon esculentum Mill.) is important food in daily human diets. Root rot disease b... more Tomato (Lycopersicon esculentum Mill.) is important food in daily human diets. Root rot disease by Fusarium oxysporum caused huge losses in tomato quality and yield annually. The extensive use of synthetic and chemical fungicides has environmental risks and health problems. Recent studies have pointed out the use of medicinal plant essential oils (EOs) and extracts for controlling fungal diseases. In the current research, Mentha spicata and Mentha longifolia EOs were used in different concentrations to control F. oxysporum. Many active compounds are present in these two EOs such as: thymol, adapic acid, menthol and menthyl acetate. These compounds possess antifungal effect through malformation and degradation of the fungal cell wall. The relative expression levels of distinctly upregulated defense-related WRKY genes (WRKY1, WRKY4, WRKY33 and WRKY53) in seedling root were evaluated as a plant-specific transcription factor (TF) group in different response pathways of abiotic stress. R...
The karyotypic characters, mitotic metaphase chromosomes and karyograms of ten populations of thr... more The karyotypic characters, mitotic metaphase chromosomes and karyograms of ten populations of three species of Plantago L. (P. lagopus L., P. major L. and P. squarrosa Murr.) in Egypt were determined and compared with each other. Analysis of somatic metaphase showed that the chromosome numbers were 2n = 12. Although all the species had the same chromosome number, they could be differentiated by their karyotype formula and quantitative parameters of the karyotypes. Ten different methods of karyotype asymmetry (TF%, As K%, Rec and Syi, A1 and A2, DI, A, AI and MCA) were determined for the elucidation of phylogenetic relationships and taxonomic treatments within a particular group or taxon. The highest value of Syi index was found in population of P. lagopus (P. lag1) collected from Abu AL-Matamir District (66.682). Population of P. squarrosa (P.sq1) collected from Qalabshu-bilqas District had the highest values of A1 (0.543), where the highest value of A2 (0.29) was recorded in popula...
Journal of Materials Research and Technology, 2021
Abstract Materials with multifunctional surface, such as UV shielding, self-cleaning or antimicro... more Abstract Materials with multifunctional surface, such as UV shielding, self-cleaning or antimicrobial properties have been highly desirable. Titanium dioxide (TiO2) and nanocomposite nanoparticles (NPs) composed of metallic silver/titanium dioxide (Ag0/TiO2) were prepared. Both of TiO2 nanoparticles and Ag0/TiO2 nanocomposite were coated onto the surface polyester fabric utilizing the straightforward pad-dry-curing technique. The morphological properties and elemental composition were determined utilizing X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), scanning electron microscope (SEM) and energy-dispersive X-ray spectroscopy (EDX). The resultant materials demonstrated photocatalytic and antimicrobial properties. The photocatalytic performance was studied using the photochemical destruction of methylene blue, while the antimicrobial activity was recorded against E. coli. The performance of polyester fabrics comprising Ag0/TiO2 nanocomposite particles was much better than those containing pure anatase TiO2 nanoparticles.
Presently, the bioprocessing of agricultural residues to various bioactive compounds is of great ... more Presently, the bioprocessing of agricultural residues to various bioactive compounds is of great concern, with the potential to be used as plant growth promoters and as a reductive of various diseases. Lycopersiconesculentum, one of the most consumed crops in the human diet, is attacked by Fusarium wilt disease, so the main aim is to biocontrol the pathogen. Several fungal species were isolated from decayed maize stover (MS). Trichodermaasperellum was chosen based on its organic acid productivity and was molecularly identified (GenBank accession number is MW195019). Citric acid (CA) was the major detected organic acid by HPLC. In vitro, CA of T.asperellum at 75% completely repressed the growth of Fusariumoxysporum f. sp. lycopersici (FOL). In vivo, soaking tomato seeds in CA enhanced the seed germination and vigor index. T. asperellum and/or its CA suppressed the wilt disease caused by FOL compared to control. There was a proportional increment of plant growth and yield, as well as ...
Genetic variability among one genotype of walnut (Juglans regia L.) and three genotypes of almond... more Genetic variability among one genotype of walnut (Juglans regia L.) and three genotypes of almond (Prunus dulcis L.) were studied by using molecular characterization. Two markers generated variability among studied cultivars RAPD & ISSR. RAPD marker produce a total number of bands 63 consists of 27 monomorphic and 36 polymorphic bands, generated polymorphism as 57.14%. Among the resulted polymorphic bands, a13 unique bands were observed. Whenever, ISSR marker generates 68 total bands with 17 monomorphic bands and 51 polymorphic bands, from these polymorphic there were 17 unique bands. Obviously, ISSR gave polymorphism among the examined plants with percentage of 75%. Data of molecular attributes computed and illustrated highest similarity coefficient among hard and scrub almond genotypes (sweet almond) and also was represented in one cluster separated the studied 4 genotypes into two groups; one group includes walnut genotype and other group includes; sweet almond (scrub hard) and b...
Tomatoes are usually consumed daily in the human diet. High temperatures reduce the number of tom... more Tomatoes are usually consumed daily in the human diet. High temperatures reduce the number of tomato yields per year. Heat stress has been considered one of the most prominent causes of alterations in morphological and molecular characteristics in crops that decrease normal growth, production, and yield in diverse plants, including tomatoes (Solanum lycopersicum L.). In this study, we evaluated six tomato lines, namely G1, G2, G3, G4, G5, and G6, at morphological, molecular, and cytological levels under heat stress. The average results of two seasons (2018 and 2019) clarified that the G6, G1, and G2 lines recorded the highest flowering values, as well as some fruit and vegetative growth traits. Furthermore, G6 and G2 had the maximum number of fruits/plant, whereas G2 and G1 produced the highest yield/plant under high temperatures. The number of chromosomes in all lines was 2n = 24, except for G5, in which the number was 2n = 26, whereas chromosome sizes were small, ranging from 323....
Irresponsible human interventions, encroachment of natural habitats, and climate change negativel... more Irresponsible human interventions, encroachment of natural habitats, and climate change negatively affect wildlife. In this study, the effects of human influence on Wadi Hagul, an unprotected area in the north of the Egyptian Eastern Desert that has recently been subjected to blatant encroachments of vegetation, were studied. The most important of these threats is the construction of the new road Al-Galala–Wadi Hagul–Zafarana. In Wadi Hagul, 80 species are reported in this study; the most represented plant families are Asteraceae (15 species) and Brassicaceae (6 species). Perennial, chamaephyte and Saharo-Arabian species were recorded in the highest percentage. Detrended canonical correspondence analysis showed that latitude, longitude, altitude, silt, sand contents, pH, and CO32− content are the factors that have the highest effect on vegetation distribution in the studied stands. Several invasive and alien species such as Euphorbia prostrata have been listed; these species typical...
Cytotoxicity Effects of Biological Control and Antioxidants using Vicia faba Chromosomal Aberration Assay, 2017
Background and Objective:
Biological control refers to purposeful utilization of introd
uced or... more Background and Objective: Biological control refers to purposeful utilization of introd uced or resident living organisms other than disease resistant host. Antioxidants protect cells against the damaging effects of reactive oxygen species otherwise called, f ree radicals such as singlet oxygen, super oxide, peroxyl radicals, hydroxyl radicals and peroxynite which results in oxidative stress lead ing to cellular damage. Cytological abnormalities in Vicia faba , studies are carried out in root tips as the using of this are a in induction of chromosomal aberrations is one of the oldest, simplest, most reliable and inexpensive method. Therefore, this study aims to determine the cytotoxic and mutagenic effect using chromosome aberration assay ( Vicia faba ). Materials and Methods: Vicia faba (broad bean) seeds (2n = 12 chromosome) (Cultivar: Giza 716), were obtained from the Agriculture Research Center, Crop Research Section, Sakha, Kafr El-Sheikh, Egypt. The seeds were prepared into slides via serial steps of fixation and squashing. Data of different treated groups of root tips were represented as Mean±SE and statically analyzed using t-test to determine the significance of the differences between treatment s and control at the 0.05 level of probability. Results: Results showed the highest percentage of MI% was 23.77% at 20 mM of citric acid for 24 h, while the lowest percentage was 9.54% at H 2 O+ Trichoderma viride for 48 h. The highest percentage of abnormal mitosis was 43.53 and 37.24% at 24 h for 20 mM of (Aa +T. harzianum ) and 3 mM of salicylic acid, respectively compared with contro l sample 9.37%, while the highest abnormal mitosis percentage at 48 h was 64.93 and 43.13% for (H 2 O+ T . viride ) and 20 mM citric acid. Conclusion: It was concluded that Trichoderma can be used safely as bio-control agents against pathogenic m icrobes. To use bioagents like antioxidants especially ascorbic acid and bio-control fungi especially T. harzianum in future in replacement of chemical fungicides (unsafe and inexpensiveness).
Toxin-contaminated foods and beverages are a major source of illness, may cause death, and have a... more Toxin-contaminated foods and beverages are a major source of illness, may cause death, and have a significant negative economic impact worldwide. Aflatoxin B1 (AFB1) is a potent toxin that may induce cancer after chronic low-level exposure. This study developed a quantitative recombinant AflR gene antiserum ELISA technique for aflatoxin B1 detection in contaminated food products. Aflatoxin B1 residuals from 36 food samples were analyzed with HPLC and VICAM. DNA was extracted from aflatoxin-contaminated samples and the AflR gene amplified using PCR. PCR products were purified and ligated into the pGEM-T vector. Recombinant plasmids were sequenced and transformed into competent E. coli (BL21). Molecular size and B-cell epitope prediction for the recombinant protein were assessed. The purified protein was used to induce the production of IgG antibodies in rabbits. Serum IgG was purified and labeled with alkaline phosphatase. Finally, indirect-ELISA was used to test the effectiveness of...
DNA barcodes have been considered as a tool to facilitate species identification based on their s... more DNA barcodes have been considered as a tool to facilitate species identification based on their simplicity and high-level accuracy compression to the complexity and subjective biases linked to morphological identification of taxa. MaturaseK gene “MatK” of the chloroplast is very crucial in the plant system which is involved in the group II intron splicing. The main objective of this current study is determining the relative utility of the “MatK” chloroplast gene for barcoding in fifteen legume trees by both single region and multiregional approaches. The chloroplast “MatK” gene sequences were submitted to GenBank and accession numbers (GenBank: LC602060, LC602154, LC602263, LC603347, LC603655, LC603845, LC603846, LC603847, LC604717, LC604718, LC605994, LC604799, LC605995, LC606468, LC606469) were obtained with sequence length ranging from 730 to 1545 nucleotides. These DNA sequences were aligned with database sequence using PROMALS server, Clustal Omega server and Bioedit program. A...
Quinoa (Chenopodium quinoa Willd.) is a halophytic crop that can withstand a variety of abiotic s... more Quinoa (Chenopodium quinoa Willd.) is a halophytic crop that can withstand a variety of abiotic stresses, including salt. The present research examined the mechanisms of salt tolerance in five different quinoa genotypes at four different salinity levels (control (60), 80, 120, and 160 mM NaCl). ISSR and SCoT analysis revealed high polymorphism percentages of 90.91% and 85.26%, respectively. Furthermore, ISSR 1 and SCoT 7 attained the greatest number of polymorphic amplicons (27 and 26), respectively. Notably, LINE-6 and M-28 genotypes demonstrated the greatest number of unique positive and negative amplicons (50 and 42) generated from ISSR and SCoT, respectively. Protein pattern analysis detected 11 bands with a polymorphism percentage 27.27% among the quinoa genotypes, with three unique bands distinguishable for the M-28 genotype. Similarity correlation indicated that the highest similarity was between S-10 and Regeolone-3 (0.657), while the lowest similarity was between M-28 and L...
Cyanobacteria comprise a good natural resource of a potential variety of neuro-chemicals, includi... more Cyanobacteria comprise a good natural resource of a potential variety of neuro-chemicals, including acetylcholinesterase inhibitors essential for Alzheimer’s disease treatment. Accordingly, eight different cyanobacterial species were isolated, identified, and evaluated on their growth on different standard nutrient media. It was found that the modified Navicula medium supported the highest growth of the test cyanobacteria. The effects of methylene chloride/methanol crude extracts of the test cyanobacteria on acetylcholinesterase activity were examined and compared. Anabaena variabilis (KU696637.1) crude extract recorded the highest acetylcholinesterase inhibition (62 ± 1.3%). Navicula medium chemical components were optimized through a Plackett–Burman factorial design. The biomass of Anabaena variabilis increased significantly when grown on the optimized medium compared to that of control. The chemical analysis of the fractions derived from Anabaena variabilis showed the presence of...
The risk of using synthetic insecticides to the environment, human health, and the emergence of n... more The risk of using synthetic insecticides to the environment, human health, and the emergence of new genera of pests resistant to that kind of drugs, have led to attention in natural compounds. The present study aimed at evaluating the insecticidal activity of 0.25–6 mg/cm2 of basil (Ocimum basilicum), black seeds (Nigella sativa), and lavender (Lavandula angustifolia) essential oils (EOs) against one of the major stored product pests, Sitophilus oryzae (L.). This was done by assessing mortality and repellent percentage assay in the adult stage, as well as analysing up and down-regulated genes associated with toxicity effect of selected EOs. The three studied EOs showed a toxic effect on S. oryzae; where O. basilicum and L. angustifolia EOs explicated 100% mortality at 6 mg/cm2 after 48 and 24 h, respectively. The highest repellence activity was recorded for O. basilicum EO at 0.75 mg/cm2 with value 82.3% after exposure time 5 h. In the highest dose (6 mg/cm2), the maximum up-regulat...
Tomato (Lycopersicon esculentum Mill.) is important food in daily human diets. Root rot disease b... more Tomato (Lycopersicon esculentum Mill.) is important food in daily human diets. Root rot disease by Fusarium oxysporum caused huge losses in tomato quality and yield annually. The extensive use of synthetic and chemical fungicides has environmental risks and health problems. Recent studies have pointed out the use of medicinal plant essential oils (EOs) and extracts for controlling fungal diseases. In the current research, Mentha spicata and Mentha longifolia EOs were used in different concentrations to control F. oxysporum. Many active compounds are present in these two EOs such as: thymol, adapic acid, menthol and menthyl acetate. These compounds possess antifungal effect through malformation and degradation of the fungal cell wall. The relative expression levels of distinctly upregulated defense-related WRKY genes (WRKY1, WRKY4, WRKY33 and WRKY53) in seedling root were evaluated as a plant-specific transcription factor (TF) group in different response pathways of abiotic stress. R...
The karyotypic characters, mitotic metaphase chromosomes and karyograms of ten populations of thr... more The karyotypic characters, mitotic metaphase chromosomes and karyograms of ten populations of three species of Plantago L. (P. lagopus L., P. major L. and P. squarrosa Murr.) in Egypt were determined and compared with each other. Analysis of somatic metaphase showed that the chromosome numbers were 2n = 12. Although all the species had the same chromosome number, they could be differentiated by their karyotype formula and quantitative parameters of the karyotypes. Ten different methods of karyotype asymmetry (TF%, As K%, Rec and Syi, A1 and A2, DI, A, AI and MCA) were determined for the elucidation of phylogenetic relationships and taxonomic treatments within a particular group or taxon. The highest value of Syi index was found in population of P. lagopus (P. lag1) collected from Abu AL-Matamir District (66.682). Population of P. squarrosa (P.sq1) collected from Qalabshu-bilqas District had the highest values of A1 (0.543), where the highest value of A2 (0.29) was recorded in popula...
Journal of Materials Research and Technology, 2021
Abstract Materials with multifunctional surface, such as UV shielding, self-cleaning or antimicro... more Abstract Materials with multifunctional surface, such as UV shielding, self-cleaning or antimicrobial properties have been highly desirable. Titanium dioxide (TiO2) and nanocomposite nanoparticles (NPs) composed of metallic silver/titanium dioxide (Ag0/TiO2) were prepared. Both of TiO2 nanoparticles and Ag0/TiO2 nanocomposite were coated onto the surface polyester fabric utilizing the straightforward pad-dry-curing technique. The morphological properties and elemental composition were determined utilizing X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), scanning electron microscope (SEM) and energy-dispersive X-ray spectroscopy (EDX). The resultant materials demonstrated photocatalytic and antimicrobial properties. The photocatalytic performance was studied using the photochemical destruction of methylene blue, while the antimicrobial activity was recorded against E. coli. The performance of polyester fabrics comprising Ag0/TiO2 nanocomposite particles was much better than those containing pure anatase TiO2 nanoparticles.
Presently, the bioprocessing of agricultural residues to various bioactive compounds is of great ... more Presently, the bioprocessing of agricultural residues to various bioactive compounds is of great concern, with the potential to be used as plant growth promoters and as a reductive of various diseases. Lycopersiconesculentum, one of the most consumed crops in the human diet, is attacked by Fusarium wilt disease, so the main aim is to biocontrol the pathogen. Several fungal species were isolated from decayed maize stover (MS). Trichodermaasperellum was chosen based on its organic acid productivity and was molecularly identified (GenBank accession number is MW195019). Citric acid (CA) was the major detected organic acid by HPLC. In vitro, CA of T.asperellum at 75% completely repressed the growth of Fusariumoxysporum f. sp. lycopersici (FOL). In vivo, soaking tomato seeds in CA enhanced the seed germination and vigor index. T. asperellum and/or its CA suppressed the wilt disease caused by FOL compared to control. There was a proportional increment of plant growth and yield, as well as ...
Genetic variability among one genotype of walnut (Juglans regia L.) and three genotypes of almond... more Genetic variability among one genotype of walnut (Juglans regia L.) and three genotypes of almond (Prunus dulcis L.) were studied by using molecular characterization. Two markers generated variability among studied cultivars RAPD & ISSR. RAPD marker produce a total number of bands 63 consists of 27 monomorphic and 36 polymorphic bands, generated polymorphism as 57.14%. Among the resulted polymorphic bands, a13 unique bands were observed. Whenever, ISSR marker generates 68 total bands with 17 monomorphic bands and 51 polymorphic bands, from these polymorphic there were 17 unique bands. Obviously, ISSR gave polymorphism among the examined plants with percentage of 75%. Data of molecular attributes computed and illustrated highest similarity coefficient among hard and scrub almond genotypes (sweet almond) and also was represented in one cluster separated the studied 4 genotypes into two groups; one group includes walnut genotype and other group includes; sweet almond (scrub hard) and b...
Tomatoes are usually consumed daily in the human diet. High temperatures reduce the number of tom... more Tomatoes are usually consumed daily in the human diet. High temperatures reduce the number of tomato yields per year. Heat stress has been considered one of the most prominent causes of alterations in morphological and molecular characteristics in crops that decrease normal growth, production, and yield in diverse plants, including tomatoes (Solanum lycopersicum L.). In this study, we evaluated six tomato lines, namely G1, G2, G3, G4, G5, and G6, at morphological, molecular, and cytological levels under heat stress. The average results of two seasons (2018 and 2019) clarified that the G6, G1, and G2 lines recorded the highest flowering values, as well as some fruit and vegetative growth traits. Furthermore, G6 and G2 had the maximum number of fruits/plant, whereas G2 and G1 produced the highest yield/plant under high temperatures. The number of chromosomes in all lines was 2n = 24, except for G5, in which the number was 2n = 26, whereas chromosome sizes were small, ranging from 323....
Irresponsible human interventions, encroachment of natural habitats, and climate change negativel... more Irresponsible human interventions, encroachment of natural habitats, and climate change negatively affect wildlife. In this study, the effects of human influence on Wadi Hagul, an unprotected area in the north of the Egyptian Eastern Desert that has recently been subjected to blatant encroachments of vegetation, were studied. The most important of these threats is the construction of the new road Al-Galala–Wadi Hagul–Zafarana. In Wadi Hagul, 80 species are reported in this study; the most represented plant families are Asteraceae (15 species) and Brassicaceae (6 species). Perennial, chamaephyte and Saharo-Arabian species were recorded in the highest percentage. Detrended canonical correspondence analysis showed that latitude, longitude, altitude, silt, sand contents, pH, and CO32− content are the factors that have the highest effect on vegetation distribution in the studied stands. Several invasive and alien species such as Euphorbia prostrata have been listed; these species typical...
Cytotoxicity Effects of Biological Control and Antioxidants using Vicia faba Chromosomal Aberration Assay, 2017
Background and Objective:
Biological control refers to purposeful utilization of introd
uced or... more Background and Objective: Biological control refers to purposeful utilization of introd uced or resident living organisms other than disease resistant host. Antioxidants protect cells against the damaging effects of reactive oxygen species otherwise called, f ree radicals such as singlet oxygen, super oxide, peroxyl radicals, hydroxyl radicals and peroxynite which results in oxidative stress lead ing to cellular damage. Cytological abnormalities in Vicia faba , studies are carried out in root tips as the using of this are a in induction of chromosomal aberrations is one of the oldest, simplest, most reliable and inexpensive method. Therefore, this study aims to determine the cytotoxic and mutagenic effect using chromosome aberration assay ( Vicia faba ). Materials and Methods: Vicia faba (broad bean) seeds (2n = 12 chromosome) (Cultivar: Giza 716), were obtained from the Agriculture Research Center, Crop Research Section, Sakha, Kafr El-Sheikh, Egypt. The seeds were prepared into slides via serial steps of fixation and squashing. Data of different treated groups of root tips were represented as Mean±SE and statically analyzed using t-test to determine the significance of the differences between treatment s and control at the 0.05 level of probability. Results: Results showed the highest percentage of MI% was 23.77% at 20 mM of citric acid for 24 h, while the lowest percentage was 9.54% at H 2 O+ Trichoderma viride for 48 h. The highest percentage of abnormal mitosis was 43.53 and 37.24% at 24 h for 20 mM of (Aa +T. harzianum ) and 3 mM of salicylic acid, respectively compared with contro l sample 9.37%, while the highest abnormal mitosis percentage at 48 h was 64.93 and 43.13% for (H 2 O+ T . viride ) and 20 mM citric acid. Conclusion: It was concluded that Trichoderma can be used safely as bio-control agents against pathogenic m icrobes. To use bioagents like antioxidants especially ascorbic acid and bio-control fungi especially T. harzianum in future in replacement of chemical fungicides (unsafe and inexpensiveness).
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Papers by Amira A Ibrahim
Biological control refers to purposeful utilization of introd
uced or resident living organisms other than
disease resistant host. Antioxidants protect cells against the damaging effects of reactive oxygen species otherwise called, f
ree radicals
such as singlet oxygen, super oxide, peroxyl radicals, hydroxyl
radicals and peroxynite which results in oxidative stress lead
ing to cellular
damage. Cytological abnormalities in
Vicia faba
, studies are carried out in root tips as the using of this are
a in induction of chromosomal
aberrations is one of the oldest, simplest, most reliable and inexpensive method. Therefore, this study aims to determine the cytotoxic
and mutagenic effect using chromosome aberration assay (
Vicia faba
).
Materials and Methods:
Vicia faba
(broad bean) seeds (2n = 12
chromosome) (Cultivar: Giza 716), were obtained from the Agriculture Research Center, Crop Research Section, Sakha, Kafr El-Sheikh,
Egypt. The seeds were prepared into slides via serial steps of
fixation and squashing. Data of different treated groups of root tips were
represented as Mean±SE and statically analyzed using t-test to
determine the significance of the differences between treatment
s and
control at the 0.05 level of probability.
Results:
Results showed the highest percentage of MI% was 23.77% at 20 mM of citric acid for
24 h, while the lowest percentage was 9.54% at H
2
O+
Trichoderma viride
for 48 h. The highest percentage of abnormal mitosis was 43.53
and 37.24% at 24 h for 20 mM of (Aa
+T. harzianum
) and 3 mM of salicylic acid, respectively compared with contro
l sample 9.37%, while
the highest abnormal mitosis percentage at 48 h was 64.93 and 43.13% for (H
2
O+
T
.
viride
) and 20 mM citric acid.
Conclusion:
It was
concluded that
Trichoderma
can be used safely as bio-control agents against pathogenic m
icrobes. To use bioagents like antioxidants
especially ascorbic acid and bio-control fungi especially
T. harzianum
in future in replacement of chemical fungicides (unsafe and
inexpensiveness).
Biological control refers to purposeful utilization of introd
uced or resident living organisms other than
disease resistant host. Antioxidants protect cells against the damaging effects of reactive oxygen species otherwise called, f
ree radicals
such as singlet oxygen, super oxide, peroxyl radicals, hydroxyl
radicals and peroxynite which results in oxidative stress lead
ing to cellular
damage. Cytological abnormalities in
Vicia faba
, studies are carried out in root tips as the using of this are
a in induction of chromosomal
aberrations is one of the oldest, simplest, most reliable and inexpensive method. Therefore, this study aims to determine the cytotoxic
and mutagenic effect using chromosome aberration assay (
Vicia faba
).
Materials and Methods:
Vicia faba
(broad bean) seeds (2n = 12
chromosome) (Cultivar: Giza 716), were obtained from the Agriculture Research Center, Crop Research Section, Sakha, Kafr El-Sheikh,
Egypt. The seeds were prepared into slides via serial steps of
fixation and squashing. Data of different treated groups of root tips were
represented as Mean±SE and statically analyzed using t-test to
determine the significance of the differences between treatment
s and
control at the 0.05 level of probability.
Results:
Results showed the highest percentage of MI% was 23.77% at 20 mM of citric acid for
24 h, while the lowest percentage was 9.54% at H
2
O+
Trichoderma viride
for 48 h. The highest percentage of abnormal mitosis was 43.53
and 37.24% at 24 h for 20 mM of (Aa
+T. harzianum
) and 3 mM of salicylic acid, respectively compared with contro
l sample 9.37%, while
the highest abnormal mitosis percentage at 48 h was 64.93 and 43.13% for (H
2
O+
T
.
viride
) and 20 mM citric acid.
Conclusion:
It was
concluded that
Trichoderma
can be used safely as bio-control agents against pathogenic m
icrobes. To use bioagents like antioxidants
especially ascorbic acid and bio-control fungi especially
T. harzianum
in future in replacement of chemical fungicides (unsafe and
inexpensiveness).