Insects change their stimulus-response through the perception of associating these stimuli with i... more Insects change their stimulus-response through the perception of associating these stimuli with important survival events such as rewards, threats, and mates. Insects develop strong associations and relate them to their experiences through several behavioral procedures. Among the insects, Apis species, Apis mellifera ligustica are known for their outstanding ability to learn with tremendous economic importance. Apis mellifera ligustica has a strong cognitive ability and promising model species for investigating the neurobiological basis of remarkable olfactory learning abilities. Here we evaluated the olfactory learning ability of A. mellifera by using the proboscis extension reflex (PER) protocol. The brains of the learner and failed-learner bees were examined for comparative transcriptome analysis by RNA-Seq to explain the difference in the learning capacity. In this study, we used an appetitive olfactory learning paradigm in the same age of A. mellifera bees to examine the differ...
Swift response models are vital tools for emergency assistance agencies. The COVID‐19 pandemic re... more Swift response models are vital tools for emergency assistance agencies. The COVID‐19 pandemic revealed the lack of economic models for short‐run policy relevant research to anticipate local impacts and design effective policy responses. The most direct effects of the pandemic and lockdown tended to be concentrated in urban areas; however, markets quickly transmitted impacts to rural areas as well as among poor and non‐poor households. General equilibrium modeling is a tool of choice to capture indirect, spillover effects of exogenous shocks. This article describes an unusual micro general‐equilibrium (GE) modeling approach that we developed to quickly simulate impacts of the pandemic and lockdowns on poor and non‐poor rural and urban households across sub‐Saharan Africa. Monte Carlo bootstrapping was used to construct four stylized regional GE models from 34 existing local economy‐wide impact evaluation (LEWIE) models. Simulations revealed that the pandemic and policy responses to ...
Abstract Wastewater irrigation in croplands is recognized as one of the major threat, seriously a... more Abstract Wastewater irrigation in croplands is recognized as one of the major threat, seriously affecting soil health and sustainable agriculture around the globe. Heavy metals presence especially cadmium (Cd) in wastewater irrigated soils is posing serious physiological and morphological disorder in plants due to its highest mobility. Therefore, metals mobility in wastewater irrigated soils can be curtailed through eco-friendly and cost effective organic soil amendments compost (CP), press mud (PM) and moringa leaf extract (ME) at 3% rate that eventually reduces its translocation from polluted soil to plant. This study explored the possible effects of various types of organic soil amendments on Cd phytoavailability in wastewater degraded soil and its subsequent accumulation in maize tissues. Maize plant was grown in Ghazi University as a test plant and Cd accumulation was recorded in its tissues, translocation from root to shoot, chlorophyll contents, plant biomass, yield and soil properties (pH, NPK, OM and Soluble Cd) were also examined. Results revealed that the addition of amendments significantly minimized Cd mobility in soil by 45.8%, 23% and 19.4% when CP, PM and ME were added at 3% over control. Comparing the control soil, Cd uptake effectively reduced via plants shoots by 33.3%, 27.7% and 19.4% when CP, PM and ME. In addition, NPK were significantly increased among all the added treatments in the soil-plant system as well as improved chlorophyll contents relative to non-treated soil. The Current study suggested that among all the amendments, compost at 3% rate performed well and can be considered a suitable approach for maize growth in polluted soil.
Proceedings of the National Academy of Sciences, 2004
Transient elevations of cytosolic Ca 2+ are a common mechanism of cellular signaling. In striated... more Transient elevations of cytosolic Ca 2+ are a common mechanism of cellular signaling. In striated muscle, the sarco(endo)plasmic reticulum Ca 2+ ATPase (SERCA) plays an important role in terminating Ca 2+ transients by returning cytosolic Ca 2+ to intracellular stores. Stored Ca 2+ can then be released again for subsequent signaling. We down-regulated SERCA2 gene expression in cultured cardiac myocytes by means of endogenous transcription of small interfering RNA encoded by an exogenous cDNA template. The cDNA template was delivered by adenovirus vector. Reduction of SERCA expression in all myocytes in culture was documented by immunochemistry, real-time RT-PCR, and determination of ATP-dependent Ca 2+ transport. The reduction of SERCA2 expression was associated with the up-regulation of transient receptor potential (TRP) channel proteins (TRPC4 and TRPC5) and Na + /Ca 2+ exchanger, indicating that intracellular store deficiency was compensated for by Ca 2+ fluxes through the plasma...
Insects change their stimulus-response through the perception of associating these stimuli with i... more Insects change their stimulus-response through the perception of associating these stimuli with important survival events such as rewards, threats, and mates. Insects develop strong associations and relate them to their experiences through several behavioral procedures. Among the insects, Apis species, Apis mellifera ligustica are known for their outstanding ability to learn with tremendous economic importance. Apis mellifera ligustica has a strong cognitive ability and promising model species for investigating the neurobiological basis of remarkable olfactory learning abilities. Here we evaluated the olfactory learning ability of A. mellifera by using the proboscis extension reflex (PER) protocol. The brains of the learner and failed-learner bees were examined for comparative transcriptome analysis by RNA-Seq to explain the difference in the learning capacity. In this study, we used an appetitive olfactory learning paradigm in the same age of A. mellifera bees to examine the differ...
Swift response models are vital tools for emergency assistance agencies. The COVID‐19 pandemic re... more Swift response models are vital tools for emergency assistance agencies. The COVID‐19 pandemic revealed the lack of economic models for short‐run policy relevant research to anticipate local impacts and design effective policy responses. The most direct effects of the pandemic and lockdown tended to be concentrated in urban areas; however, markets quickly transmitted impacts to rural areas as well as among poor and non‐poor households. General equilibrium modeling is a tool of choice to capture indirect, spillover effects of exogenous shocks. This article describes an unusual micro general‐equilibrium (GE) modeling approach that we developed to quickly simulate impacts of the pandemic and lockdowns on poor and non‐poor rural and urban households across sub‐Saharan Africa. Monte Carlo bootstrapping was used to construct four stylized regional GE models from 34 existing local economy‐wide impact evaluation (LEWIE) models. Simulations revealed that the pandemic and policy responses to ...
Abstract Wastewater irrigation in croplands is recognized as one of the major threat, seriously a... more Abstract Wastewater irrigation in croplands is recognized as one of the major threat, seriously affecting soil health and sustainable agriculture around the globe. Heavy metals presence especially cadmium (Cd) in wastewater irrigated soils is posing serious physiological and morphological disorder in plants due to its highest mobility. Therefore, metals mobility in wastewater irrigated soils can be curtailed through eco-friendly and cost effective organic soil amendments compost (CP), press mud (PM) and moringa leaf extract (ME) at 3% rate that eventually reduces its translocation from polluted soil to plant. This study explored the possible effects of various types of organic soil amendments on Cd phytoavailability in wastewater degraded soil and its subsequent accumulation in maize tissues. Maize plant was grown in Ghazi University as a test plant and Cd accumulation was recorded in its tissues, translocation from root to shoot, chlorophyll contents, plant biomass, yield and soil properties (pH, NPK, OM and Soluble Cd) were also examined. Results revealed that the addition of amendments significantly minimized Cd mobility in soil by 45.8%, 23% and 19.4% when CP, PM and ME were added at 3% over control. Comparing the control soil, Cd uptake effectively reduced via plants shoots by 33.3%, 27.7% and 19.4% when CP, PM and ME. In addition, NPK were significantly increased among all the added treatments in the soil-plant system as well as improved chlorophyll contents relative to non-treated soil. The Current study suggested that among all the amendments, compost at 3% rate performed well and can be considered a suitable approach for maize growth in polluted soil.
Proceedings of the National Academy of Sciences, 2004
Transient elevations of cytosolic Ca 2+ are a common mechanism of cellular signaling. In striated... more Transient elevations of cytosolic Ca 2+ are a common mechanism of cellular signaling. In striated muscle, the sarco(endo)plasmic reticulum Ca 2+ ATPase (SERCA) plays an important role in terminating Ca 2+ transients by returning cytosolic Ca 2+ to intracellular stores. Stored Ca 2+ can then be released again for subsequent signaling. We down-regulated SERCA2 gene expression in cultured cardiac myocytes by means of endogenous transcription of small interfering RNA encoded by an exogenous cDNA template. The cDNA template was delivered by adenovirus vector. Reduction of SERCA expression in all myocytes in culture was documented by immunochemistry, real-time RT-PCR, and determination of ATP-dependent Ca 2+ transport. The reduction of SERCA2 expression was associated with the up-regulation of transient receptor potential (TRP) channel proteins (TRPC4 and TRPC5) and Na + /Ca 2+ exchanger, indicating that intracellular store deficiency was compensated for by Ca 2+ fluxes through the plasma...
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Papers by Arif Nur Husain