A study was conducted using 16 rhizobial strains to examine the occurrence and distribution of rh... more A study was conducted using 16 rhizobial strains to examine the occurrence and distribution of rhizobiophages in 80 soil samples collected from the rhizosphere of different leguminous and non-leguminous plants at various locations in the Nile Valley, Egypt. Phages were detected ...
... DOI: 10.1080/03650340410001663909 M Fayez a * , SH Shehata Heba b , GA El-Morsy c , ARahal b ... more ... DOI: 10.1080/03650340410001663909 M Fayez a * , SH Shehata Heba b , GA El-Morsy c , ARahal b & AF ... Hegazi NA Hamza-Mervat A Osman A Ali S Sedik MZ Fayez M 1998 A modified-combined carbon N-deficient medium for isolation, enumeration and mass production ...
Shiga toxin-producing E. coli (STEC) is considered a worldwide public health and food safety prob... more Shiga toxin-producing E. coli (STEC) is considered a worldwide public health and food safety problem. Despite the implementation of various different approaches to control food safety, outbreaks persist. The aim of study is to evaluate the applicability of phages, isolated against STEC O157:H7, as prospective food bio-preservatives. Considering the relatively wide host range and greatest protein diversity, two phages (STEC P2 and P4) from four were furtherly characterized. Complete genome analysis confirmed the absence of toxins and virulence factors—encoding genes. The results confirmed the close relation of STEC P2 to phages of Myoviridae, and STEC P4 to the Podoviridae family. The phages retained higher lytic competence of 90.4 and 92.68% for STEC P2 and P4, respectively with the HTST pasteurization. The strong acidic (pH 1) and alkaline (pH 13) conditions had influential effect on the surviving counts of the two phages. The lowest survivability of 63.37 and 86.36% in STEC P2 and...
Hydrocarbon degradation in soil by microorganisms has undergone renewed emphasis because of the i... more Hydrocarbon degradation in soil by microorganisms has undergone renewed emphasis because of the increased incidence of petroleum-based pollution. Knowledge about hydrocarbon degradation is needed to determine how microorganisms might be utilized in the removal of the pollutants from the environment. Bioremediation is a modern method in which the natural ability of microorganisms is employed for the reduction of the concentration and/or toxicity of various chemical substances, such as petroleum derivatives, aliphatic and aromatic hydrocarbons, industrial solvents, pesticides and metals. The class ISSN: 2319-7706 Volume 3 Number 4 (2014) pp. 401-414 http://www.ijcmas.com
Twenty seven potential hydrocarbon degrader bacterial isolates were isolated from soil that has b... more Twenty seven potential hydrocarbon degrader bacterial isolates were isolated from soil that has been exposed to crude petroleum oil. The hydrocarbon degradation potentialities of these isolates were assessed against n-tetradecane. Four isolates (AT3, AT5, AT11 and AT15) were the most potent isolates. These isolates were identified via morphological, biochemical and 16S-rRNA gene sequencing techniques as (Pseudomonas stutzeri, Bacillus thuringiensis, Bacillus pumilus and Bacillus cereus), respectively. AT3 isolate was the highest n-tetradecane degrader. This isolate exhibited specific growth rate in Bushnell-Hass medium amended with hydrocarbon, n-tetradecane of 1.213 h -1 and growth yield of 1.311 g cells g hydrocarbon -1 . An efficient biodegradation up to 90% was performed by AT3 bacterial isolate. Genetic fingerprinting was carried out using RAPD-PCR and SDS-PAGE methods to characterize and conduct phylogenetic relationship among the four most potent isolates. Thirty six specific...
Hydrocarbon degradation in soil by microorganisms has undergone renewed emphasis because of the i... more Hydrocarbon degradation in soil by microorganisms has undergone renewed emphasis because of the increased incidence of petroleum-based pollution. Knowledge about hydrocarbon degradation is needed to determine how microorganisms might be utilized in the removal of the pollutants from the environment. Bioremediation is a modern method in which the natural ability of microorganisms is employed for the reduction of the concentration and/or toxicity of various chemical substances, such as petroleum derivatives, aliphatic and aromatic hydrocarbons, industrial solvents, pesticides and metals. The class ISSN: 2319-7706 Volume 3 Number 4 (2014) pp. 401-414 http://www.ijcmas.com
Bioremediation is an efficient strategy for cleaning up sites contaminated with organic pollutant... more Bioremediation is an efficient strategy for cleaning up sites contaminated with organic pollutants. Oil is worldwide contaminant that can reach toxic concentrations that are detrimental to the environment and human health. The present study investigated bioremediation, by gravimetric analysis, the ability of theoil-degrading bacteria to produce biosurfecants. Four bacterial strains were used in the study. Strains were local isolated strains by enrichment culture technique from polluted sites in industrial area of Taif, Saudi Arabia. These isolates were identified through morphological, biochemical and 16S-rRNA gene sequencing techniques as Bacillus thuringiensis AT5, Actinomyces octodloyts AF104, Pseduomonas aerogenosa AF11-GT and Pseduomonas stutzeri AT3.Their ability to degrade crude oil hydrocarbon were monitored by gravimetric assay for 5 days. The total viable count of isolates ranged from 1.7 x10 4 - 2.1x10 6 CFU mL -1 culture. The four strains showed relatively high capacity ...
Goat (Capra aegagrus), as one of the ancient livestock, was first domesticated in Western Asia in... more Goat (Capra aegagrus), as one of the ancient livestock, was first domesticated in Western Asia in the period of 9000 7000BC (Zenuner 1963). It was considered that the domestic goat breeds in the world were from a single origin and their separations in different ecological areas (Nozawa 1988). Zhang and Li (1990) suggested that the goat in the world could be divided into three populations according to the Hb gene frequency (Li et al., 2004). Generally, goat breeds are named by their places of origin and classified by their uses of products. Asthe effect of different ecological conditions and long-term artificial selection, goat breeds with different genetic characteristics and product orientation are formed (Yue 2000).The largest number of goats is observed in Asia, followed by Africa, representing about 59.7% and 33.8%, summing up to 93.5% out of the total number of the world, Dairy goats ISSN: 2319-7706 Volume 4 Number 4 (2015) pp. 223-231 http://www.ijcmas.com
Rhizobiophages are considered one of the most important biological factors negatively affecting t... more Rhizobiophages are considered one of the most important biological factors negatively affecting the numbers and activity of rhizobia. They directly lead to lysis of rhizobial cells resulted in reducing their population in soil. In addition, they indirectly affect the ability of rhizobia to fix nitrogen due to the formation of phageresistant strains which have less or no nitrogen fixation capacity. Rhizobiophages were isolated from different sources such as soils, nodules, roots, stems and cultures ISSN: 2319-7706 Volume 3 Number 5 (2014) pp. 155-171 http://www.ijcmas.com
This study aimed to evaluate the effect of altitude on some male fertility related traits in Saud... more This study aimed to evaluate the effect of altitude on some male fertility related traits in Saudi sheep and goats. Testicular tissues were collected from a slaughterhouse in Taif governorate (1800 m above sea level) and Makkah governorate (sea level). Sperm characteristics (1 - individual motility, 2 - percent live sperm 3 - percent abnormal sperm) were examined. Semi-quantitative RT -PCR assay was used to evaluate the expression of IGF-II, StAR, LDLr and CYP11A genes. No significant effect of altitude on tested sperm parameters was revealed. Expression of IGF-II gene in both sheep and goats was significantly (P<0.05) higher at sea level compared to high altitude. A similar effect of altitude on StAR gene expression was only observed in goats, while in sheep the level of effect did not reach the significance threshold. Moreover, LDLr gene expression was significantly (P<0.05) higher for both sheep and goats at high altitude than at sea level. The CYP11A gene expression was si...
A study was conducted using 16 rhizobial strains to examine the occurrence and distribution of rh... more A study was conducted using 16 rhizobial strains to examine the occurrence and distribution of rhizobiophages in 80 soil samples collected from the rhizosphere of different leguminous and non-leguminous plants at various locations in the Nile Valley, Egypt. Phages were detected ...
... DOI: 10.1080/03650340410001663909 M Fayez a * , SH Shehata Heba b , GA El-Morsy c , ARahal b ... more ... DOI: 10.1080/03650340410001663909 M Fayez a * , SH Shehata Heba b , GA El-Morsy c , ARahal b &amp;amp; AF ... Hegazi NA Hamza-Mervat A Osman A Ali S Sedik MZ Fayez M 1998 A modified-combined carbon N-deficient medium for isolation, enumeration and mass production ...
Shiga toxin-producing E. coli (STEC) is considered a worldwide public health and food safety prob... more Shiga toxin-producing E. coli (STEC) is considered a worldwide public health and food safety problem. Despite the implementation of various different approaches to control food safety, outbreaks persist. The aim of study is to evaluate the applicability of phages, isolated against STEC O157:H7, as prospective food bio-preservatives. Considering the relatively wide host range and greatest protein diversity, two phages (STEC P2 and P4) from four were furtherly characterized. Complete genome analysis confirmed the absence of toxins and virulence factors—encoding genes. The results confirmed the close relation of STEC P2 to phages of Myoviridae, and STEC P4 to the Podoviridae family. The phages retained higher lytic competence of 90.4 and 92.68% for STEC P2 and P4, respectively with the HTST pasteurization. The strong acidic (pH 1) and alkaline (pH 13) conditions had influential effect on the surviving counts of the two phages. The lowest survivability of 63.37 and 86.36% in STEC P2 and...
Hydrocarbon degradation in soil by microorganisms has undergone renewed emphasis because of the i... more Hydrocarbon degradation in soil by microorganisms has undergone renewed emphasis because of the increased incidence of petroleum-based pollution. Knowledge about hydrocarbon degradation is needed to determine how microorganisms might be utilized in the removal of the pollutants from the environment. Bioremediation is a modern method in which the natural ability of microorganisms is employed for the reduction of the concentration and/or toxicity of various chemical substances, such as petroleum derivatives, aliphatic and aromatic hydrocarbons, industrial solvents, pesticides and metals. The class ISSN: 2319-7706 Volume 3 Number 4 (2014) pp. 401-414 http://www.ijcmas.com
Twenty seven potential hydrocarbon degrader bacterial isolates were isolated from soil that has b... more Twenty seven potential hydrocarbon degrader bacterial isolates were isolated from soil that has been exposed to crude petroleum oil. The hydrocarbon degradation potentialities of these isolates were assessed against n-tetradecane. Four isolates (AT3, AT5, AT11 and AT15) were the most potent isolates. These isolates were identified via morphological, biochemical and 16S-rRNA gene sequencing techniques as (Pseudomonas stutzeri, Bacillus thuringiensis, Bacillus pumilus and Bacillus cereus), respectively. AT3 isolate was the highest n-tetradecane degrader. This isolate exhibited specific growth rate in Bushnell-Hass medium amended with hydrocarbon, n-tetradecane of 1.213 h -1 and growth yield of 1.311 g cells g hydrocarbon -1 . An efficient biodegradation up to 90% was performed by AT3 bacterial isolate. Genetic fingerprinting was carried out using RAPD-PCR and SDS-PAGE methods to characterize and conduct phylogenetic relationship among the four most potent isolates. Thirty six specific...
Hydrocarbon degradation in soil by microorganisms has undergone renewed emphasis because of the i... more Hydrocarbon degradation in soil by microorganisms has undergone renewed emphasis because of the increased incidence of petroleum-based pollution. Knowledge about hydrocarbon degradation is needed to determine how microorganisms might be utilized in the removal of the pollutants from the environment. Bioremediation is a modern method in which the natural ability of microorganisms is employed for the reduction of the concentration and/or toxicity of various chemical substances, such as petroleum derivatives, aliphatic and aromatic hydrocarbons, industrial solvents, pesticides and metals. The class ISSN: 2319-7706 Volume 3 Number 4 (2014) pp. 401-414 http://www.ijcmas.com
Bioremediation is an efficient strategy for cleaning up sites contaminated with organic pollutant... more Bioremediation is an efficient strategy for cleaning up sites contaminated with organic pollutants. Oil is worldwide contaminant that can reach toxic concentrations that are detrimental to the environment and human health. The present study investigated bioremediation, by gravimetric analysis, the ability of theoil-degrading bacteria to produce biosurfecants. Four bacterial strains were used in the study. Strains were local isolated strains by enrichment culture technique from polluted sites in industrial area of Taif, Saudi Arabia. These isolates were identified through morphological, biochemical and 16S-rRNA gene sequencing techniques as Bacillus thuringiensis AT5, Actinomyces octodloyts AF104, Pseduomonas aerogenosa AF11-GT and Pseduomonas stutzeri AT3.Their ability to degrade crude oil hydrocarbon were monitored by gravimetric assay for 5 days. The total viable count of isolates ranged from 1.7 x10 4 - 2.1x10 6 CFU mL -1 culture. The four strains showed relatively high capacity ...
Goat (Capra aegagrus), as one of the ancient livestock, was first domesticated in Western Asia in... more Goat (Capra aegagrus), as one of the ancient livestock, was first domesticated in Western Asia in the period of 9000 7000BC (Zenuner 1963). It was considered that the domestic goat breeds in the world were from a single origin and their separations in different ecological areas (Nozawa 1988). Zhang and Li (1990) suggested that the goat in the world could be divided into three populations according to the Hb gene frequency (Li et al., 2004). Generally, goat breeds are named by their places of origin and classified by their uses of products. Asthe effect of different ecological conditions and long-term artificial selection, goat breeds with different genetic characteristics and product orientation are formed (Yue 2000).The largest number of goats is observed in Asia, followed by Africa, representing about 59.7% and 33.8%, summing up to 93.5% out of the total number of the world, Dairy goats ISSN: 2319-7706 Volume 4 Number 4 (2015) pp. 223-231 http://www.ijcmas.com
Rhizobiophages are considered one of the most important biological factors negatively affecting t... more Rhizobiophages are considered one of the most important biological factors negatively affecting the numbers and activity of rhizobia. They directly lead to lysis of rhizobial cells resulted in reducing their population in soil. In addition, they indirectly affect the ability of rhizobia to fix nitrogen due to the formation of phageresistant strains which have less or no nitrogen fixation capacity. Rhizobiophages were isolated from different sources such as soils, nodules, roots, stems and cultures ISSN: 2319-7706 Volume 3 Number 5 (2014) pp. 155-171 http://www.ijcmas.com
This study aimed to evaluate the effect of altitude on some male fertility related traits in Saud... more This study aimed to evaluate the effect of altitude on some male fertility related traits in Saudi sheep and goats. Testicular tissues were collected from a slaughterhouse in Taif governorate (1800 m above sea level) and Makkah governorate (sea level). Sperm characteristics (1 - individual motility, 2 - percent live sperm 3 - percent abnormal sperm) were examined. Semi-quantitative RT -PCR assay was used to evaluate the expression of IGF-II, StAR, LDLr and CYP11A genes. No significant effect of altitude on tested sperm parameters was revealed. Expression of IGF-II gene in both sheep and goats was significantly (P<0.05) higher at sea level compared to high altitude. A similar effect of altitude on StAR gene expression was only observed in goats, while in sheep the level of effect did not reach the significance threshold. Moreover, LDLr gene expression was significantly (P<0.05) higher for both sheep and goats at high altitude than at sea level. The CYP11A gene expression was si...
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