Individuals of blood group O and nonsecretors of ABO blood group antigens are more susceptible to... more Individuals of blood group O and nonsecretors of ABO blood group antigens are more susceptible to peptic ulcers. The aim of this study was to determine if blood group antigens associated with group O or secretor status are epithelial cell receptors for Helicobacter pylori. Bacterial binding and binding of monoclonal antibodies to H type 2, Lewis(a), and Lewis(b) to Kato III, buccal epithelial, and gastric mucosal cells were shown by flow cytometry. Bacterial outer membrane proteins eluted from H type 2, Lewis(a), or Lewis(b) were shown by polyacrylamide gel electrophoresis. Kato III and human epithelial cells bound each monoclonal antibody; O cells bound more anti-H type 2 (P < 0.05). Binding indices for H. pylori correlated with those for anti-H type 2 (P < 0.005) and anti-Lewis(b) (P < 0.001) but not anti-Lewis(a). A 61-kilodalton protein was eluted from H type 2, Lewis(a), or Lewis(b). Our results indicate that H type 2 is an important receptor for the 61-kilodalton bacterial adhesin, partly explaining increased susceptibility of individuals of blood group O to ulcers. Lewis(b) binds H. pylori more efficiently than Lewis(a). If these interactions occur in vivo, lack of Lewis(b) in mucosal fluids of nonsecretors may contribute to colonization by H. pylori.
Introduction: Urinary Tract infection (UTI) is considered as the most common infections disease a... more Introduction: Urinary Tract infection (UTI) is considered as the most common infections disease among women. UTI may lead to a serious complication in both kidneys. In this study, we tried to determine whether the alternation in platelet count during UTI is related to specific Gram-type of bacteria causing the infection. This assists the medical staff in selection of suitable antimicrobial drug. Material and Methods: Data of all urine cultures from Gynecology Department/ Albaraa Hospital/Tripoli processed at the Laboratory during a period of three years (2017 - 2019). All Data analysis and statistics were made with the SPSS software version 16 and performed with one-way ANOVA and a parametric test was used for categorical values and not normally distributed values. Results: A growth was detected in 101 of 193 (52%) samples and 22 (11%) were mixed growth. Gram-negative bacteria identified in 57% (45/79) while 43% (34/79) occurred with Gram-positive bacteria. Regarding to platelets co...
Introduction: Susceptibility to some infectious diseases has been associated with blood group phe... more Introduction: Susceptibility to some infectious diseases has been associated with blood group phenotypes. Coronavirus disease 2019 (COVID-19) is a new infectious disease caused by a novel corona virus designated Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) which has spread rapidly around the world. The objective of this study was to determine if the susceptibility or severity to COVID-19 was associated with ABO blood group distribution among the affected countries. Materials and methods: This retrospective study of COVID-19 disease was based on data from 105 countries collected on the 13th of April 2020 and analysed by SPSS software version 16.0. Spearman correlation coefficient and Kruskal Wallis test were used to assess for associations between ABO blood group with COVID-19. Results: The results indicated that individuals of blood group A are at increased risk of infection by SARS-CoV-2 virus and severity of COVID-19 disease. Blood groups B and O were less likely t...
Introduction: Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is spreading rapidly a... more Introduction: Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is spreading rapidly around the world caused serious respiratory disease leading to death, therefore vaccine is the effective way to control this pandemic. Scientists tried to produce safe and effective SARS-CoV-2 vaccines in record time and vaccination program started in December 2020. The aim of this study to determine the impact of SARS-CoV-2 vaccine on COVID-19 disease. Materials and methods: The COVID-19 cases data of post-vaccination and pre-vaccination were collected from Worldmeter website, and the percentage of vaccinated people data obtained from Statistics and Research Coronavirus Vaccinations. The statistical analyses performed with SPSS software version 16.0. Results: The results showed countries with higher percentage of full dose vaccinated people correlated strongly with decrease of death and the severity of the disease among their infected population. This study showed that different vaccine ...
 Title: Prevalence of bacterial infection among hospital traumatic patients in relation to ABO ... more  Title: Prevalence of bacterial infection among hospital traumatic patients in relation to ABO blood group. Background: There are many studies demonstrated a correlation between blood group antigens and susceptibility to infectious diseases such as bacteria, parasites and viruses. Objectives: To assess the prevalence of bacterial infection among patients in the trauma hospital, and to assess the susceptibility of ABO blood groups to the isolated bacteria.  Methods and Findings: 166 samples included, wound swabs, sputum and midstream urine were received for routine culture diagnostic procedures from the in-patients at Abosleem Traumatic Hospital and ABO group was obtained from Blood bank documented system for each patient. A correlation between isolated organisms and ABO system was determined. 51% patients were infected during their stay in the hospital by one of the following isolates: Pseudomonas (22%); Klebsiella (9%); Staphylococci (15%); and Streptococci (4%). The majority...
CHAPTER 11 Blood group phenotypes and infectious diseases C Caroline Blackwell Discipline of Immu... more CHAPTER 11 Blood group phenotypes and infectious diseases C Caroline Blackwell Discipline of Immunology and Microbiology and Hunter Immunology Unit, University of Newcastle, Newcastle, Australia; Institute for Scientific Evaluation of Naturopathy, University of ...
Sudden Infant Death Syndrome (SIDS) remains the major cause of post neonatal mortality in countri... more Sudden Infant Death Syndrome (SIDS) remains the major cause of post neonatal mortality in countries where thorough autopsies and investigations of these deaths occur. The incidence of SIDS is higher among some ethnic groups, and among these groups there are also high incidences of serious infectious diseases. The risk factors for SIDS parallel those for susceptibility to infection. One current hypothesis for SIDS is that the deaths are due to overwhelming pro- inflammatory responses to bacterial toxins. During inflammatory responses to sepsis or toxins, the cytokines induced in response to the infectious agent cause physiological changes leading to death. The genetic, developmental and environmental risk factors for SIDS are reviewed in relation to 1) colonisation of infants by potentially harmful bacteria and 2) induction of the inflammatory responses of non-immune infants to micro-organisms or their products.
Individuals of blood group O and nonsecretors of ABO blood group antigens are more susceptible to... more Individuals of blood group O and nonsecretors of ABO blood group antigens are more susceptible to peptic ulcers. The aim of this study was to determine if blood group antigens associated with group O or secretor status are epithelial cell receptors for Helicobacter pylori. Bacterial binding and binding of monoclonal antibodies to H type 2, Lewis(a), and Lewis(b) to Kato III, buccal epithelial, and gastric mucosal cells were shown by flow cytometry. Bacterial outer membrane proteins eluted from H type 2, Lewis(a), or Lewis(b) were shown by polyacrylamide gel electrophoresis. Kato III and human epithelial cells bound each monoclonal antibody; O cells bound more anti-H type 2 (P < 0.05). Binding indices for H. pylori correlated with those for anti-H type 2 (P < 0.005) and anti-Lewis(b) (P < 0.001) but not anti-Lewis(a). A 61-kilodalton protein was eluted from H type 2, Lewis(a), or Lewis(b). Our results indicate that H type 2 is an important receptor for the 61-kilodalton bacterial adhesin, partly explaining increased susceptibility of individuals of blood group O to ulcers. Lewis(b) binds H. pylori more efficiently than Lewis(a). If these interactions occur in vivo, lack of Lewis(b) in mucosal fluids of nonsecretors may contribute to colonization by H. pylori.
Persons of blood group O are at increased risk of peptic ulcers. Enhanced binding of Helicobacter... more Persons of blood group O are at increased risk of peptic ulcers. Enhanced binding of Helicobacter pylori to epithelial cells of persons of blood group O has been demonstrated. Release of interleukin (IL)-6, IL-10, and tumor necrosis factor (TNF)-alpha by human leukocytes from 40 donors (10 from each ABO blood group) was measured after incubation in vitro with outer membrane protein preparations of H. pylori. Isolates DU (from a patient with a duodenal ulcer), GC (from a patient with gastric cancer), NE (from a patient with normal endoscopic findings), and NCTC 11637 bound in significantly higher numbers to group O leukocytes. Bacterial binding correlated with release of IL-6 and TNF-alpha but not of IL-10. Group O cells released significantly more IL-6 in response to DU, NE, and NCTC 11637, and the cells released more TNF-alpha in response to DU and NCTC 11637. Increased density of colonization of epithelial cells and higher inflammatory responses to H. pylori of persons of blood group O might contribute to increased susceptibility to peptic ulceration.
Individuals of blood group O and nonsecretors of ABO blood group antigens are more susceptible to... more Individuals of blood group O and nonsecretors of ABO blood group antigens are more susceptible to peptic ulcers. The aim of this study was to determine if blood group antigens associated with group O or secretor status are epithelial cell receptors for Helicobacter pylori. Bacterial binding and binding of monoclonal antibodies to H type 2, Lewis(a), and Lewis(b) to Kato III, buccal epithelial, and gastric mucosal cells were shown by flow cytometry. Bacterial outer membrane proteins eluted from H type 2, Lewis(a), or Lewis(b) were shown by polyacrylamide gel electrophoresis. Kato III and human epithelial cells bound each monoclonal antibody; O cells bound more anti-H type 2 (P < 0.05). Binding indices for H. pylori correlated with those for anti-H type 2 (P < 0.005) and anti-Lewis(b) (P < 0.001) but not anti-Lewis(a). A 61-kilodalton protein was eluted from H type 2, Lewis(a), or Lewis(b). Our results indicate that H type 2 is an important receptor for the 61-kilodalton bacterial adhesin, partly explaining increased susceptibility of individuals of blood group O to ulcers. Lewis(b) binds H. pylori more efficiently than Lewis(a). If these interactions occur in vivo, lack of Lewis(b) in mucosal fluids of nonsecretors may contribute to colonization by H. pylori.
Introduction: Urinary Tract infection (UTI) is considered as the most common infections disease a... more Introduction: Urinary Tract infection (UTI) is considered as the most common infections disease among women. UTI may lead to a serious complication in both kidneys. In this study, we tried to determine whether the alternation in platelet count during UTI is related to specific Gram-type of bacteria causing the infection. This assists the medical staff in selection of suitable antimicrobial drug. Material and Methods: Data of all urine cultures from Gynecology Department/ Albaraa Hospital/Tripoli processed at the Laboratory during a period of three years (2017 - 2019). All Data analysis and statistics were made with the SPSS software version 16 and performed with one-way ANOVA and a parametric test was used for categorical values and not normally distributed values. Results: A growth was detected in 101 of 193 (52%) samples and 22 (11%) were mixed growth. Gram-negative bacteria identified in 57% (45/79) while 43% (34/79) occurred with Gram-positive bacteria. Regarding to platelets co...
Introduction: Susceptibility to some infectious diseases has been associated with blood group phe... more Introduction: Susceptibility to some infectious diseases has been associated with blood group phenotypes. Coronavirus disease 2019 (COVID-19) is a new infectious disease caused by a novel corona virus designated Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) which has spread rapidly around the world. The objective of this study was to determine if the susceptibility or severity to COVID-19 was associated with ABO blood group distribution among the affected countries. Materials and methods: This retrospective study of COVID-19 disease was based on data from 105 countries collected on the 13th of April 2020 and analysed by SPSS software version 16.0. Spearman correlation coefficient and Kruskal Wallis test were used to assess for associations between ABO blood group with COVID-19. Results: The results indicated that individuals of blood group A are at increased risk of infection by SARS-CoV-2 virus and severity of COVID-19 disease. Blood groups B and O were less likely t...
Introduction: Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is spreading rapidly a... more Introduction: Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is spreading rapidly around the world caused serious respiratory disease leading to death, therefore vaccine is the effective way to control this pandemic. Scientists tried to produce safe and effective SARS-CoV-2 vaccines in record time and vaccination program started in December 2020. The aim of this study to determine the impact of SARS-CoV-2 vaccine on COVID-19 disease. Materials and methods: The COVID-19 cases data of post-vaccination and pre-vaccination were collected from Worldmeter website, and the percentage of vaccinated people data obtained from Statistics and Research Coronavirus Vaccinations. The statistical analyses performed with SPSS software version 16.0. Results: The results showed countries with higher percentage of full dose vaccinated people correlated strongly with decrease of death and the severity of the disease among their infected population. This study showed that different vaccine ...
 Title: Prevalence of bacterial infection among hospital traumatic patients in relation to ABO ... more  Title: Prevalence of bacterial infection among hospital traumatic patients in relation to ABO blood group. Background: There are many studies demonstrated a correlation between blood group antigens and susceptibility to infectious diseases such as bacteria, parasites and viruses. Objectives: To assess the prevalence of bacterial infection among patients in the trauma hospital, and to assess the susceptibility of ABO blood groups to the isolated bacteria.  Methods and Findings: 166 samples included, wound swabs, sputum and midstream urine were received for routine culture diagnostic procedures from the in-patients at Abosleem Traumatic Hospital and ABO group was obtained from Blood bank documented system for each patient. A correlation between isolated organisms and ABO system was determined. 51% patients were infected during their stay in the hospital by one of the following isolates: Pseudomonas (22%); Klebsiella (9%); Staphylococci (15%); and Streptococci (4%). The majority...
CHAPTER 11 Blood group phenotypes and infectious diseases C Caroline Blackwell Discipline of Immu... more CHAPTER 11 Blood group phenotypes and infectious diseases C Caroline Blackwell Discipline of Immunology and Microbiology and Hunter Immunology Unit, University of Newcastle, Newcastle, Australia; Institute for Scientific Evaluation of Naturopathy, University of ...
Sudden Infant Death Syndrome (SIDS) remains the major cause of post neonatal mortality in countri... more Sudden Infant Death Syndrome (SIDS) remains the major cause of post neonatal mortality in countries where thorough autopsies and investigations of these deaths occur. The incidence of SIDS is higher among some ethnic groups, and among these groups there are also high incidences of serious infectious diseases. The risk factors for SIDS parallel those for susceptibility to infection. One current hypothesis for SIDS is that the deaths are due to overwhelming pro- inflammatory responses to bacterial toxins. During inflammatory responses to sepsis or toxins, the cytokines induced in response to the infectious agent cause physiological changes leading to death. The genetic, developmental and environmental risk factors for SIDS are reviewed in relation to 1) colonisation of infants by potentially harmful bacteria and 2) induction of the inflammatory responses of non-immune infants to micro-organisms or their products.
Individuals of blood group O and nonsecretors of ABO blood group antigens are more susceptible to... more Individuals of blood group O and nonsecretors of ABO blood group antigens are more susceptible to peptic ulcers. The aim of this study was to determine if blood group antigens associated with group O or secretor status are epithelial cell receptors for Helicobacter pylori. Bacterial binding and binding of monoclonal antibodies to H type 2, Lewis(a), and Lewis(b) to Kato III, buccal epithelial, and gastric mucosal cells were shown by flow cytometry. Bacterial outer membrane proteins eluted from H type 2, Lewis(a), or Lewis(b) were shown by polyacrylamide gel electrophoresis. Kato III and human epithelial cells bound each monoclonal antibody; O cells bound more anti-H type 2 (P < 0.05). Binding indices for H. pylori correlated with those for anti-H type 2 (P < 0.005) and anti-Lewis(b) (P < 0.001) but not anti-Lewis(a). A 61-kilodalton protein was eluted from H type 2, Lewis(a), or Lewis(b). Our results indicate that H type 2 is an important receptor for the 61-kilodalton bacterial adhesin, partly explaining increased susceptibility of individuals of blood group O to ulcers. Lewis(b) binds H. pylori more efficiently than Lewis(a). If these interactions occur in vivo, lack of Lewis(b) in mucosal fluids of nonsecretors may contribute to colonization by H. pylori.
Persons of blood group O are at increased risk of peptic ulcers. Enhanced binding of Helicobacter... more Persons of blood group O are at increased risk of peptic ulcers. Enhanced binding of Helicobacter pylori to epithelial cells of persons of blood group O has been demonstrated. Release of interleukin (IL)-6, IL-10, and tumor necrosis factor (TNF)-alpha by human leukocytes from 40 donors (10 from each ABO blood group) was measured after incubation in vitro with outer membrane protein preparations of H. pylori. Isolates DU (from a patient with a duodenal ulcer), GC (from a patient with gastric cancer), NE (from a patient with normal endoscopic findings), and NCTC 11637 bound in significantly higher numbers to group O leukocytes. Bacterial binding correlated with release of IL-6 and TNF-alpha but not of IL-10. Group O cells released significantly more IL-6 in response to DU, NE, and NCTC 11637, and the cells released more TNF-alpha in response to DU and NCTC 11637. Increased density of colonization of epithelial cells and higher inflammatory responses to H. pylori of persons of blood group O might contribute to increased susceptibility to peptic ulceration.
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