To identify an analgesic/anti-inflammatory component from the leaves of Rhododendron aureum (Eric... more To identify an analgesic/anti-inflammatory component from the leaves of Rhododendron aureum (Ericaceae), phytochemical isolation and pharmacological assays (writhing assays and vascular permeability assay for analgesic action in mice; carrageenan-induced paw edemaand TPA-induced ear edema assays of anti-inflammatory action in rats) were performed. Four compounds were isolated from the active fraction (BuOH fraction) by silica gel column chromatography and identified as (−)-rhododendrol, (−)-rhododendrin, avicularin and hyperoside by spectroscopic methods. Rhododendrin, the main compound of the BuOH fraction, exhibited significant analgesic actions in mice and anti-inflammatory actions in rats. This compound accounted for 3.1% of the MeOH extract and 0.48% of dried leaves, respectively, on HPLC analysis. These results suggest that rhododendrin is the major biologically active substance in the leaves of R. aureum with analgesic/anti-inflammatory activity.
Young and mature Artemisia princeps var. orientalis (APO, Compositae) are used as a health food a... more Young and mature Artemisia princeps var. orientalis (APO, Compositae) are used as a health food and a medicinal plant, respectively, in Korea. Here, we identified the in vitro potent peroxynitrite (ONOO−)-scavenging effect (IC50, 0.26 μg/mL) of the components from the EtOAc fraction. Octadecylsilane column chromatography on the EtOAc fraction yielded two caffeoylquinic acid compounds, 3,5-di-O-caffeoyl-muco-quinic acid (1) and methyl 4,5-di-O-caffeoylquinate (2) by NMR spectroscopic data, which have not been reported before from APO. The IC50 values of compounds 1 and 2 were 0.18 ± 0.01 μg/mL and 0.12 ± 0.00 μg/mL, respectively, lower than that of the positive control (L-penicillamine). HPLC data indicated that young APO (1: 30.3 mg/g dried weight, 2: 27.7 mg/g) contained considerably higher quantities of the two caffeoylquinic acids than mature APO (1: 1.77 mg/g dried weight, 2: 4.10 mg/g).
Two new flavonol glycosides, kaempferol 3-O-[beta-D-glucopyranosyl-(1--&a... more Two new flavonol glycosides, kaempferol 3-O-[beta-D-glucopyranosyl-(1-->4)][alpha-L-rhamnopyranosyl-(1-->6)]-beta-D-glucopyranoside and quercetin 3-O-[beta-D-glucopyranosyl-(1-->4)][alpha-L-rhamnopyranosyl-(1-->6)]-beta-D-glucopyranoside, together with three known flavonoids were isolated using column chromatography from the aerial parts of Lamium amplexicaule (Labiatae). In addition, the five isolates were evaluated for their in vitro free radical scavenging (EC50 values, 14.1-63.9 microg/mL) and tyrosinase inhibitory activities (IC50 values, 110.4-193.5 microg/mL).
Mountainous vegetables called chwinamul are used in Korea to promote health. Chwinamul was obtain... more Mountainous vegetables called chwinamul are used in Korea to promote health. Chwinamul was obtained from several plants belonging to the Compositae — e.g., Kalimeris yomena, Aster scaber, Solidago virga var. gigantea, Solidago viragaurea var. asiatica, Saussurea grandifolia, Ainsliaea acerifolia — were used for our experiments. Analytical methods for simultaneous determination of the caffeoylquinic acids (3,4-di-O-caffeoylquinic acid, 3,5-di-O-dicaffeoyl-epi-quinic acid, 3,5-di-O-caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid, 5-O-caffeoylquinic acid, 3-O-caffeoylquinic acid, 3-O-p-coumaroyl-caffeoylquinic acids) were established for chwinamul. The kinds of constituents were identified from HPLC chromatograms and it was possible to calculate the percentage (w/w) of seven of these compounds in the dried plants and in the extracts. The proportion of caffeoylquinic acids in the extracts ranged from 20.25 to 38.35%. Since it is known that peroxynitrite (ONOO−)-scavenging is beneficial for amelioration of obesity, diabetes mellitus, atherosclerosis and even Alzheimer’s disease, assays for peroxynitrite-scavenging activity were performed on the seven chwinamul plants. Of the tested extracts, the MeOH extract of A. acerifolia had the most potent effect (IC50 1.49 ± 0.68 µg/mL). These results suggest that chwinamul vegetables can be used for treatment or prevention of peroxynitrite-related diseases.
To identify an analgesic/anti-inflammatory component from the leaves of Rhododendron aureum (Eric... more To identify an analgesic/anti-inflammatory component from the leaves of Rhododendron aureum (Ericaceae), phytochemical isolation and pharmacological assays (writhing assays and vascular permeability assay for analgesic action in mice; carrageenan-induced paw edemaand TPA-induced ear edema assays of anti-inflammatory action in rats) were performed. Four compounds were isolated from the active fraction (BuOH fraction) by silica gel column chromatography and identified as (−)-rhododendrol, (−)-rhododendrin, avicularin and hyperoside by spectroscopic methods. Rhododendrin, the main compound of the BuOH fraction, exhibited significant analgesic actions in mice and anti-inflammatory actions in rats. This compound accounted for 3.1% of the MeOH extract and 0.48% of dried leaves, respectively, on HPLC analysis. These results suggest that rhododendrin is the major biologically active substance in the leaves of R. aureum with analgesic/anti-inflammatory activity.
Young and mature Artemisia princeps var. orientalis (APO, Compositae) are used as a health food a... more Young and mature Artemisia princeps var. orientalis (APO, Compositae) are used as a health food and a medicinal plant, respectively, in Korea. Here, we identified the in vitro potent peroxynitrite (ONOO−)-scavenging effect (IC50, 0.26 μg/mL) of the components from the EtOAc fraction. Octadecylsilane column chromatography on the EtOAc fraction yielded two caffeoylquinic acid compounds, 3,5-di-O-caffeoyl-muco-quinic acid (1) and methyl 4,5-di-O-caffeoylquinate (2) by NMR spectroscopic data, which have not been reported before from APO. The IC50 values of compounds 1 and 2 were 0.18 ± 0.01 μg/mL and 0.12 ± 0.00 μg/mL, respectively, lower than that of the positive control (L-penicillamine). HPLC data indicated that young APO (1: 30.3 mg/g dried weight, 2: 27.7 mg/g) contained considerably higher quantities of the two caffeoylquinic acids than mature APO (1: 1.77 mg/g dried weight, 2: 4.10 mg/g).
Two new flavonol glycosides, kaempferol 3-O-[beta-D-glucopyranosyl-(1--&a... more Two new flavonol glycosides, kaempferol 3-O-[beta-D-glucopyranosyl-(1-->4)][alpha-L-rhamnopyranosyl-(1-->6)]-beta-D-glucopyranoside and quercetin 3-O-[beta-D-glucopyranosyl-(1-->4)][alpha-L-rhamnopyranosyl-(1-->6)]-beta-D-glucopyranoside, together with three known flavonoids were isolated using column chromatography from the aerial parts of Lamium amplexicaule (Labiatae). In addition, the five isolates were evaluated for their in vitro free radical scavenging (EC50 values, 14.1-63.9 microg/mL) and tyrosinase inhibitory activities (IC50 values, 110.4-193.5 microg/mL).
Mountainous vegetables called chwinamul are used in Korea to promote health. Chwinamul was obtain... more Mountainous vegetables called chwinamul are used in Korea to promote health. Chwinamul was obtained from several plants belonging to the Compositae — e.g., Kalimeris yomena, Aster scaber, Solidago virga var. gigantea, Solidago viragaurea var. asiatica, Saussurea grandifolia, Ainsliaea acerifolia — were used for our experiments. Analytical methods for simultaneous determination of the caffeoylquinic acids (3,4-di-O-caffeoylquinic acid, 3,5-di-O-dicaffeoyl-epi-quinic acid, 3,5-di-O-caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid, 5-O-caffeoylquinic acid, 3-O-caffeoylquinic acid, 3-O-p-coumaroyl-caffeoylquinic acids) were established for chwinamul. The kinds of constituents were identified from HPLC chromatograms and it was possible to calculate the percentage (w/w) of seven of these compounds in the dried plants and in the extracts. The proportion of caffeoylquinic acids in the extracts ranged from 20.25 to 38.35%. Since it is known that peroxynitrite (ONOO−)-scavenging is beneficial for amelioration of obesity, diabetes mellitus, atherosclerosis and even Alzheimer’s disease, assays for peroxynitrite-scavenging activity were performed on the seven chwinamul plants. Of the tested extracts, the MeOH extract of A. acerifolia had the most potent effect (IC50 1.49 ± 0.68 µg/mL). These results suggest that chwinamul vegetables can be used for treatment or prevention of peroxynitrite-related diseases.
Uploads