In Vitro Cellular & Developmental Biology. …, 1995
In Vitro Cell. Dev. Biol. 31:140-148, February 1995 @ 1995 Society for In Vitro Biology 1071-2690... more In Vitro Cell. Dev. Biol. 31:140-148, February 1995 @ 1995 Society for In Vitro Biology 1071-2690/95 $02.50+0.00 ... CHARACTERIZATION AND CO-CULTURE OF NOVEL NONTRANSFORMED CELL LINES DERIVED FROM RAT ENDOMETRIAL EPITHELIUM AND STROMA
M.-F Bader N. Balthasar G. Banhegyi W. Banks P. Barbry C. Barcia S. W. Barger P. A. Barker G. Bar... more M.-F Bader N. Balthasar G. Banhegyi W. Banks P. Barbry C. Barcia S. W. Barger P. A. Barker G. Baron T. Bartfai R. Bartrons A. N. Bateson G. Battaglia K.-U. Bayer C.-M. Becker M. Beer F. Benfenati J. Bentley D. Berg M. Bergeron J. Berkeley J. Bernal M. Bernaudin A. Bérod J. R. Bethea K. Bhakoo P. Bickford P. E. Bickler S. Bilcker L. Binder T. Binz R. D. Blakely E. Blazquez J. K. Blusztajn E. Bock S. Boehm J. P. Bolaños N. M. Bonini K. L. Bost M. Bourin C. Bradberry O. Braissant S. Braithwaite N. Brakch M. D. Brand D. S. Bredt R. J. Bridges J.-P. Brion E. Brodde S. Broer P. S. Brookes J. Brotchie M. Browning A. J. Bruce-Keller V. Bruno N. J. Buckley P. Bungay A. Butt D. A. Butterfield
Introduction. Despite the widespread use of prostaglandin E1 as an efficacious treatment for mal... more Introduction. Despite the widespread use of prostaglandin E1 as an efficacious treatment for male erectile dysfunction for more than two decades, research on prostanoid function in penile physiology has been limited.Aim. To characterize the pharmacological and physiological activity of novel subtype-selective EP and DP receptor agonists.Methods. Radioligand binding and second messenger assays were used to define receptor subtype specificity of the EP and DP agonists. Functional activity was further characterized using isolated human and rabbit penile cavernosal tissue in organ baths. In vivo activity was assessed in rabbits and rats by measuring changes in cavernous pressure after intracavernosal injection of receptor agonists.Main Outcome Measures. Receptor binding and signal transduction, smooth muscle contractile activity, erectile function.Results. In organ bath preparations of human cavernosal tissue contracted with phenylephrine, EP2- and EP4-selective agonists exhibited ...
Using a video-imaging technique, we characterized the effects of 8-isoprostaglandin F 2α (8-iso-P... more Using a video-imaging technique, we characterized the effects of 8-isoprostaglandin F 2α (8-iso-PGF 2α ) on retinal vasculature from piglets. 8-Iso-PGF 2α potently contracted (EC 50 = 5.9 ± 0.5 nM) retinal vessels. These effects were completely antagonized by the ...
Dehydration represents a strong stimulus for the secretory activity of hypothalamic vasopressiner... more Dehydration represents a strong stimulus for the secretory activity of hypothalamic vasopressinergic neurons and induces a sustained rise in hypothalamic [Arg8]vasopressin (AVP) mRNA. We now report that, in addition to the change in mRNA accumulation, this stimulus leads to an increase in the size of the mature AVP gene transcripts. Using Northern blot analysis in conjunction with internal RNA size markers, we demonstrate here that a 6-day period of salt imbibition induced a gradual size increase of AVP mRNA from 810 to a maximum of 930 bases. This change was fully reversed by day 30 following termination of the salt treatment. Any alteration of the mRNA capping or polyadenylation sites was ruled out by S1 mapping. However, poly(A) tail removal reduced AVP mRNA of all treatment groups to exactly the same size (600 bases). It is concluded that the dehydration-induced size change is due to a greater than 50% increase of the mean steady state length of the poly(A) tail. It remains to be determined to what extent the observed increase in poly(A) tail length may have an effect on AVP mRNA stability or translational efficiency.
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 1998
Using a video-imaging technique, we characterized the effects of 8-isoprostaglandin F2α(8-iso-PGF... more Using a video-imaging technique, we characterized the effects of 8-isoprostaglandin F2α(8-iso-PGF2α) on retinal vasculature from piglets. 8-Iso-PGF2αpotently contracted (EC50= 5.9 ± 0.5 nM) retinal vessels. These effects were completely antagonized by the cyclooxygenase inhibitor indomethacin, the thromboxane synthase blocker CGS-12970, the thromboxane receptor antagonist L-670596, and the putative inhibitor of the non-voltage-dependent receptor-operated Ca2+pathway SKF-96365; constrictor effects of 8-iso-PGF2αwere also partly attenuated by the ETA-receptor blocker BQ-123 and an inhibitor of endothelin-converting enzyme, phosphoramidon, but was negligibly affected by the L-type voltage-gated Ca2+channel blocker nifedipine. Correspondingly, 8-iso-PGF2αelicited endothelin release from retinal preparations, which was markedly reduced by SKF-96365. 8-Iso-PGF2αalso increased thromboxane production in the retina and cultured endothelial cells, but not on retinovascular smooth muscle cells...
Microvascular degeneration is an important event in oxygen-induced retinopathy (OIR), a model of ... more Microvascular degeneration is an important event in oxygen-induced retinopathy (OIR), a model of retinopathy of prematurity. Because oxidant stress abundantly generates thromboxane A2(TxA2), we tested whether TxA2plays a role in retinal vasoobliteration of OIR and contributes to such vascular degeneration by direct endothelial cytotoxicity. Hyperoxia-induced retinal vasoobliteration in rat pups (80% O2exposure from postnatal days 5–14) was associated with increased TxB2generation and was significantly prevented by TxA2synthase inhibitor CGS-12970 (10 mg · kg−1· day−1) or TxA2-receptor antagonist CGS-22652 (10 mg · kg−1· day−1). TxA2mimetics U-46619 (EC5050 nM) and I-BOP (EC505 nM) caused a time- and concentration-dependent cell death of neuroretinovascular endothelial cells from rats as well as newborn pigs but not of smooth muscle and astroglial cells; other prostanoids did not cause cell death. The peroxidation product 8-iso-PGF2, which is generated in OIR, stimulated TxA2formatio...
Cellular and Molecular Biology of Myelination, 1990
Advances in recombinant DNA technologies have given us the capacity to introduce (by transfection... more Advances in recombinant DNA technologies have given us the capacity to introduce (by transfection), with relative ease, cDNAs encoding expressible polypeptides into host cells. In these novel intracellular environments, properties of the encoded proteins may be revealed that are not easily studied when the same proteins are expressed in their natural surroundings. Using the transfection paradigm, information about the mechanism of polymerization of the individual neurofilament proteins can be obtained in cells such as Cos and L tk− cells that express a variety of other cytoskeletal proteins (Chin and Liem, 1989). The proteins involved in myelin formation may also be studied in this way. In myelinogenesis, the interactions between membrane lipids and proteins that lead to the assembly of the multilamellar myelin sheath are extremely complex. However, by transfecting cDNAs encoding the individual myelin proteins into non-glial cells that act as “foreign” hosts, the intracellular behavior and fate of each myelin protein (in the absence of the others) can be monitored, and data interpreted in light of what is known about myelin assembly from other studies. Moreover, the existence of rodent myelin mutants that express at null or very low levels certain structural genes of myelin (Campagnoni and Macklin, 1988) potentially allows us to introduce into the mutant myelin-forming cells “minigenes” encoding the missing complete polypeptides or only small segments thereof.
The Journal of pharmacology and experimental therapeutics, 1996
The present study was done to determine possible ontogenic differences in the functions of rat my... more The present study was done to determine possible ontogenic differences in the functions of rat myocardial alpha1, adrenoceptor (alpha1 AR) subtypes in view of reported greater inotropic responses of myocardium of neonatal than of adult rats to alpha1, AR agonists. Methoxamine, phenylephrine and norepinephrine were used as alpha1 AR agonists. Phenylephrine and norepinephrine were used in the presence of 3 microM propranolol. It was found that the ratios of chloroethylclonidine (CEC)-insensitive alpha1 AR subtype (alpha1A AR) to CES-sensitive alpha, AR subtype (alpha1B AR) were approximately 50:50 in neonatal (1 week old) and 20:80 in adult rat ventricles. alpha1A AR selective antagonists WB 4101 and 5-methylurapidil+ (5-MU) inhibited the inotropic effects of alpha1, AR agonists both on neonatal and on adult rat ventricles; in contrast, selective inactivation of alpha1B AR by CEC inhibited the inotropic effects of alpha1 AR agonists only on ventricles from adult but not from neonatal ...
Novel injectable chitosan sponges based on rapid ionic crosslinking using guanosine 5&amp... more Novel injectable chitosan sponges based on rapid ionic crosslinking using guanosine 5'-diphosphate are introduced. The rapid gelation, high water retention, desirable physicochemical properties, soft tissue-like mechanical properties, and excellent cytocompatibility make these injectable sponges promising candidates for tissue regeneration and drug delivery applications.
A. Gaillard P. Gailly E. Galea R. Garcez G. Garden P. Gardner F. Gardoni C. Gautier J. Geiger N. ... more A. Gaillard P. Gailly E. Galea R. Garcez G. Garden P. Gardner F. Gardoni C. Gautier J. Geiger N. Gerges C. Ghiani C. Gibson G. Gibson J. Gidday D. Gietzen R. Giger G. Gille S. D. Ginsberg R. Gonzales The Chief and Deputy Chief Editors, on behalf of the Editorial Board and the International Society for Neurochemistry, acknowledge with thanks the following, who assisted them by providing reviews in the period between April 1st and June 30th, 2012. JOURNAL OF NEUROCHEMISTRY | 2012 | 122 | 1230–1232 doi: 10.1111/j.1471-4159.2011.07880.x
The synthesis of myelin basic protein is severely reduced in the central and peripheral nervous s... more The synthesis of myelin basic protein is severely reduced in the central and peripheral nervous system of myelin-deficient (mld) mutant mice. Using an in vitro system and immunoprecipitation, we found a normal rate of synthesis in mld mice for two intrinsic myelin proteins: proteolipid protein in central nervous system myelin and P0 in peripheral nervous system myelin. These results indicate that protein synthesis on membrane-bound ribosomes is not affected by the mutation.
The influence of dexamethasone on the development of neurons and oligodendrocytes was studied in ... more The influence of dexamethasone on the development of neurons and oligodendrocytes was studied in serum-free, aggregating rat brain cell cultures. Synaptogenesis and myelination occur in this culture system. The concentration of myelin basic protein and the activity of 2',3'-cyclic nucleotide 3'-phosphodiesterase were used as oligodendroglia and myelin markers. Choline acetyltransferase and acetylcholinesterase served as neuronal markers, glutamine synthetase reflected astrocyte differentiation, while ornithine decarboxylase served as a general marker for cell growth and maturation. This study showed that dexamethasone stimulated the differentiation of cholinergic neurons and astrocytes. The effect of dexamethasone on oligodendroglial differentiation and myelination depended on the stage of development: during the early phase of myelination dexamethasone had a stimulatory effect, whereas at a later stage it showed a significant inhibition.
In Vitro Cellular & Developmental Biology. …, 1995
In Vitro Cell. Dev. Biol. 31:140-148, February 1995 @ 1995 Society for In Vitro Biology 1071-2690... more In Vitro Cell. Dev. Biol. 31:140-148, February 1995 @ 1995 Society for In Vitro Biology 1071-2690/95 $02.50+0.00 ... CHARACTERIZATION AND CO-CULTURE OF NOVEL NONTRANSFORMED CELL LINES DERIVED FROM RAT ENDOMETRIAL EPITHELIUM AND STROMA
M.-F Bader N. Balthasar G. Banhegyi W. Banks P. Barbry C. Barcia S. W. Barger P. A. Barker G. Bar... more M.-F Bader N. Balthasar G. Banhegyi W. Banks P. Barbry C. Barcia S. W. Barger P. A. Barker G. Baron T. Bartfai R. Bartrons A. N. Bateson G. Battaglia K.-U. Bayer C.-M. Becker M. Beer F. Benfenati J. Bentley D. Berg M. Bergeron J. Berkeley J. Bernal M. Bernaudin A. Bérod J. R. Bethea K. Bhakoo P. Bickford P. E. Bickler S. Bilcker L. Binder T. Binz R. D. Blakely E. Blazquez J. K. Blusztajn E. Bock S. Boehm J. P. Bolaños N. M. Bonini K. L. Bost M. Bourin C. Bradberry O. Braissant S. Braithwaite N. Brakch M. D. Brand D. S. Bredt R. J. Bridges J.-P. Brion E. Brodde S. Broer P. S. Brookes J. Brotchie M. Browning A. J. Bruce-Keller V. Bruno N. J. Buckley P. Bungay A. Butt D. A. Butterfield
Introduction. Despite the widespread use of prostaglandin E1 as an efficacious treatment for mal... more Introduction. Despite the widespread use of prostaglandin E1 as an efficacious treatment for male erectile dysfunction for more than two decades, research on prostanoid function in penile physiology has been limited.Aim. To characterize the pharmacological and physiological activity of novel subtype-selective EP and DP receptor agonists.Methods. Radioligand binding and second messenger assays were used to define receptor subtype specificity of the EP and DP agonists. Functional activity was further characterized using isolated human and rabbit penile cavernosal tissue in organ baths. In vivo activity was assessed in rabbits and rats by measuring changes in cavernous pressure after intracavernosal injection of receptor agonists.Main Outcome Measures. Receptor binding and signal transduction, smooth muscle contractile activity, erectile function.Results. In organ bath preparations of human cavernosal tissue contracted with phenylephrine, EP2- and EP4-selective agonists exhibited ...
Using a video-imaging technique, we characterized the effects of 8-isoprostaglandin F 2α (8-iso-P... more Using a video-imaging technique, we characterized the effects of 8-isoprostaglandin F 2α (8-iso-PGF 2α ) on retinal vasculature from piglets. 8-Iso-PGF 2α potently contracted (EC 50 = 5.9 ± 0.5 nM) retinal vessels. These effects were completely antagonized by the ...
Dehydration represents a strong stimulus for the secretory activity of hypothalamic vasopressiner... more Dehydration represents a strong stimulus for the secretory activity of hypothalamic vasopressinergic neurons and induces a sustained rise in hypothalamic [Arg8]vasopressin (AVP) mRNA. We now report that, in addition to the change in mRNA accumulation, this stimulus leads to an increase in the size of the mature AVP gene transcripts. Using Northern blot analysis in conjunction with internal RNA size markers, we demonstrate here that a 6-day period of salt imbibition induced a gradual size increase of AVP mRNA from 810 to a maximum of 930 bases. This change was fully reversed by day 30 following termination of the salt treatment. Any alteration of the mRNA capping or polyadenylation sites was ruled out by S1 mapping. However, poly(A) tail removal reduced AVP mRNA of all treatment groups to exactly the same size (600 bases). It is concluded that the dehydration-induced size change is due to a greater than 50% increase of the mean steady state length of the poly(A) tail. It remains to be determined to what extent the observed increase in poly(A) tail length may have an effect on AVP mRNA stability or translational efficiency.
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 1998
Using a video-imaging technique, we characterized the effects of 8-isoprostaglandin F2α(8-iso-PGF... more Using a video-imaging technique, we characterized the effects of 8-isoprostaglandin F2α(8-iso-PGF2α) on retinal vasculature from piglets. 8-Iso-PGF2αpotently contracted (EC50= 5.9 ± 0.5 nM) retinal vessels. These effects were completely antagonized by the cyclooxygenase inhibitor indomethacin, the thromboxane synthase blocker CGS-12970, the thromboxane receptor antagonist L-670596, and the putative inhibitor of the non-voltage-dependent receptor-operated Ca2+pathway SKF-96365; constrictor effects of 8-iso-PGF2αwere also partly attenuated by the ETA-receptor blocker BQ-123 and an inhibitor of endothelin-converting enzyme, phosphoramidon, but was negligibly affected by the L-type voltage-gated Ca2+channel blocker nifedipine. Correspondingly, 8-iso-PGF2αelicited endothelin release from retinal preparations, which was markedly reduced by SKF-96365. 8-Iso-PGF2αalso increased thromboxane production in the retina and cultured endothelial cells, but not on retinovascular smooth muscle cells...
Microvascular degeneration is an important event in oxygen-induced retinopathy (OIR), a model of ... more Microvascular degeneration is an important event in oxygen-induced retinopathy (OIR), a model of retinopathy of prematurity. Because oxidant stress abundantly generates thromboxane A2(TxA2), we tested whether TxA2plays a role in retinal vasoobliteration of OIR and contributes to such vascular degeneration by direct endothelial cytotoxicity. Hyperoxia-induced retinal vasoobliteration in rat pups (80% O2exposure from postnatal days 5–14) was associated with increased TxB2generation and was significantly prevented by TxA2synthase inhibitor CGS-12970 (10 mg · kg−1· day−1) or TxA2-receptor antagonist CGS-22652 (10 mg · kg−1· day−1). TxA2mimetics U-46619 (EC5050 nM) and I-BOP (EC505 nM) caused a time- and concentration-dependent cell death of neuroretinovascular endothelial cells from rats as well as newborn pigs but not of smooth muscle and astroglial cells; other prostanoids did not cause cell death. The peroxidation product 8-iso-PGF2, which is generated in OIR, stimulated TxA2formatio...
Cellular and Molecular Biology of Myelination, 1990
Advances in recombinant DNA technologies have given us the capacity to introduce (by transfection... more Advances in recombinant DNA technologies have given us the capacity to introduce (by transfection), with relative ease, cDNAs encoding expressible polypeptides into host cells. In these novel intracellular environments, properties of the encoded proteins may be revealed that are not easily studied when the same proteins are expressed in their natural surroundings. Using the transfection paradigm, information about the mechanism of polymerization of the individual neurofilament proteins can be obtained in cells such as Cos and L tk− cells that express a variety of other cytoskeletal proteins (Chin and Liem, 1989). The proteins involved in myelin formation may also be studied in this way. In myelinogenesis, the interactions between membrane lipids and proteins that lead to the assembly of the multilamellar myelin sheath are extremely complex. However, by transfecting cDNAs encoding the individual myelin proteins into non-glial cells that act as “foreign” hosts, the intracellular behavior and fate of each myelin protein (in the absence of the others) can be monitored, and data interpreted in light of what is known about myelin assembly from other studies. Moreover, the existence of rodent myelin mutants that express at null or very low levels certain structural genes of myelin (Campagnoni and Macklin, 1988) potentially allows us to introduce into the mutant myelin-forming cells “minigenes” encoding the missing complete polypeptides or only small segments thereof.
The Journal of pharmacology and experimental therapeutics, 1996
The present study was done to determine possible ontogenic differences in the functions of rat my... more The present study was done to determine possible ontogenic differences in the functions of rat myocardial alpha1, adrenoceptor (alpha1 AR) subtypes in view of reported greater inotropic responses of myocardium of neonatal than of adult rats to alpha1, AR agonists. Methoxamine, phenylephrine and norepinephrine were used as alpha1 AR agonists. Phenylephrine and norepinephrine were used in the presence of 3 microM propranolol. It was found that the ratios of chloroethylclonidine (CEC)-insensitive alpha1 AR subtype (alpha1A AR) to CES-sensitive alpha, AR subtype (alpha1B AR) were approximately 50:50 in neonatal (1 week old) and 20:80 in adult rat ventricles. alpha1A AR selective antagonists WB 4101 and 5-methylurapidil+ (5-MU) inhibited the inotropic effects of alpha1, AR agonists both on neonatal and on adult rat ventricles; in contrast, selective inactivation of alpha1B AR by CEC inhibited the inotropic effects of alpha1 AR agonists only on ventricles from adult but not from neonatal ...
Novel injectable chitosan sponges based on rapid ionic crosslinking using guanosine 5&amp... more Novel injectable chitosan sponges based on rapid ionic crosslinking using guanosine 5'-diphosphate are introduced. The rapid gelation, high water retention, desirable physicochemical properties, soft tissue-like mechanical properties, and excellent cytocompatibility make these injectable sponges promising candidates for tissue regeneration and drug delivery applications.
A. Gaillard P. Gailly E. Galea R. Garcez G. Garden P. Gardner F. Gardoni C. Gautier J. Geiger N. ... more A. Gaillard P. Gailly E. Galea R. Garcez G. Garden P. Gardner F. Gardoni C. Gautier J. Geiger N. Gerges C. Ghiani C. Gibson G. Gibson J. Gidday D. Gietzen R. Giger G. Gille S. D. Ginsberg R. Gonzales The Chief and Deputy Chief Editors, on behalf of the Editorial Board and the International Society for Neurochemistry, acknowledge with thanks the following, who assisted them by providing reviews in the period between April 1st and June 30th, 2012. JOURNAL OF NEUROCHEMISTRY | 2012 | 122 | 1230–1232 doi: 10.1111/j.1471-4159.2011.07880.x
The synthesis of myelin basic protein is severely reduced in the central and peripheral nervous s... more The synthesis of myelin basic protein is severely reduced in the central and peripheral nervous system of myelin-deficient (mld) mutant mice. Using an in vitro system and immunoprecipitation, we found a normal rate of synthesis in mld mice for two intrinsic myelin proteins: proteolipid protein in central nervous system myelin and P0 in peripheral nervous system myelin. These results indicate that protein synthesis on membrane-bound ribosomes is not affected by the mutation.
The influence of dexamethasone on the development of neurons and oligodendrocytes was studied in ... more The influence of dexamethasone on the development of neurons and oligodendrocytes was studied in serum-free, aggregating rat brain cell cultures. Synaptogenesis and myelination occur in this culture system. The concentration of myelin basic protein and the activity of 2',3'-cyclic nucleotide 3'-phosphodiesterase were used as oligodendroglia and myelin markers. Choline acetyltransferase and acetylcholinesterase served as neuronal markers, glutamine synthetase reflected astrocyte differentiation, while ornithine decarboxylase served as a general marker for cell growth and maturation. This study showed that dexamethasone stimulated the differentiation of cholinergic neurons and astrocytes. The effect of dexamethasone on oligodendroglial differentiation and myelination depended on the stage of development: during the early phase of myelination dexamethasone had a stimulatory effect, whereas at a later stage it showed a significant inhibition.
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