El dengue es una de las principales enfermedades virales de carácter epidémico. Constituye la arb... more El dengue es una de las principales enfermedades virales de carácter epidémico. Constituye la arbovirosis más importante a nivel mundial en morbilidad, mortalidad e importancia económica. Casi la mitad de la población mundial se encuentra en riesgo. En México, el dengue es una de las principales enfermedades transmitidas por vector, y en los últimos diez años ha incrementado el número de casos a pesar de los esfuerzos de prevención y control del vector. Objetivo. Describir la infección por dengue, así como las dificultades técnicas y de participación ciudadana para la erradicación de este problema de Salud Pública en México. Metodología. Se realizó una revisión sistemática de literatura de los aspectos generales de la infección por dengue y las dificultades técnicas y de participación comunitaria que se presentan para su erradicación. Se eligieron artículos científicos desde 1995 hasta 2016, los cuales se identificaron en las bases de datos Medline, Pubmed, Scielo y Redalyc por medi...
Alzheimer’s disease (AD) is a neurodegenerative disease, characterized histopathologically by int... more Alzheimer’s disease (AD) is a neurodegenerative disease, characterized histopathologically by intra-neuronal tau-related lesions and by the accumulation of amyloid β-peptide (Aβ) in the brain parenchyma and around cerebral blood vessels. According to the vascular hypothesis of AD, an alteration in the neurovascular unit (NVU) could lead to Aβ vascular accumulation and promote neuronal dysfunction, accelerating neurodegeneration and dementia. To date, the effects of insoluble vascular Aβ deposits on the NVU and the blood–brain barrier (BBB) are unknown. In this study, we analyze different Aβ species and their association with the cells that make up the NVU. We evaluated post-mortem AD brain tissue. Multiple immunofluorescence assays were performed against different species of Aβ and the main elements that constitute the NVU. Our results showed that there are insoluble vascular deposits of both full-length and truncated Aβ species. Besides, insoluble aggregates are associated with a d...
Vesicular trafficking, which is implicated in secretion of cytolytic molecules as well as in phag... more Vesicular trafficking, which is implicated in secretion of cytolytic molecules as well as in phagocytosis, plays an important role in the pathogenic mechanism of Entamoeba histolytica, the protozoan parasite causative of human amoebiasis. Thus, Rab GTPases, that are key regulators of vesicle trafficking, should be considered as molecules involved in the parasite virulence. EhRabB is a Rab protein located in cytoplasmic vesicles that are translocated to phagocytic mouths during ingestion of target cells, suggesting that this Rab protein is involved in phagocytosis. To prove this hypothesis, we over expressed the wild type EhrabB gene and a mutant gene encoding for a protein (RabBN118I) unable to bind guanine nucleotides and therefore constitutively inactive. The over expression of the mutated protein in E. histolytica trophozoites provoked a dominant negative effect, reflected in a significant decrease of both phagocytosis and cytopathic effect as well as in a failure to produce hepatic abscesses in hamsters. These results confirm that EhRabB is involved in phagocytosis and virulence of E. histolytica.
El dengue es una de las principales enfermedades virales de carácter epidémico. Constituye la arb... more El dengue es una de las principales enfermedades virales de carácter epidémico. Constituye la arbovirosis más importante a nivel mundial en morbilidad, mortalidad e importancia económica. Casi la mitad de la población mundial se encuentra en riesgo. En México, el dengue es una de las principales enfermedades transmitidas por vector, y en los últimos diez años ha incrementado el número de casos a pesar de los esfuerzos de prevención y control del vector. Objetivo. Describir la infección por dengue, así como las dificultades técnicas y de participación ciudadana para la erradicación de este problema de Salud Pública en México. Metodología. Se realizó una revisión sistemática de literatura de los aspectos generales de la infección por dengue y las dificultades técnicas y de participación comunitaria que se presentan para su erradicación. Se eligieron artículos científicos desde 1995 hasta 2016, los cuales se identificaron en las bases de datos Medline, Pubmed, Scielo y Redalyc por medi...
Alzheimer’s disease (AD) is a neurodegenerative disease, characterized histopathologically by int... more Alzheimer’s disease (AD) is a neurodegenerative disease, characterized histopathologically by intra-neuronal tau-related lesions and by the accumulation of amyloid β-peptide (Aβ) in the brain parenchyma and around cerebral blood vessels. According to the vascular hypothesis of AD, an alteration in the neurovascular unit (NVU) could lead to Aβ vascular accumulation and promote neuronal dysfunction, accelerating neurodegeneration and dementia. To date, the effects of insoluble vascular Aβ deposits on the NVU and the blood–brain barrier (BBB) are unknown. In this study, we analyze different Aβ species and their association with the cells that make up the NVU. We evaluated post-mortem AD brain tissue. Multiple immunofluorescence assays were performed against different species of Aβ and the main elements that constitute the NVU. Our results showed that there are insoluble vascular deposits of both full-length and truncated Aβ species. Besides, insoluble aggregates are associated with a d...
Vesicular trafficking, which is implicated in secretion of cytolytic molecules as well as in phag... more Vesicular trafficking, which is implicated in secretion of cytolytic molecules as well as in phagocytosis, plays an important role in the pathogenic mechanism of Entamoeba histolytica, the protozoan parasite causative of human amoebiasis. Thus, Rab GTPases, that are key regulators of vesicle trafficking, should be considered as molecules involved in the parasite virulence. EhRabB is a Rab protein located in cytoplasmic vesicles that are translocated to phagocytic mouths during ingestion of target cells, suggesting that this Rab protein is involved in phagocytosis. To prove this hypothesis, we over expressed the wild type EhrabB gene and a mutant gene encoding for a protein (RabBN118I) unable to bind guanine nucleotides and therefore constitutively inactive. The over expression of the mutated protein in E. histolytica trophozoites provoked a dominant negative effect, reflected in a significant decrease of both phagocytosis and cytopathic effect as well as in a failure to produce hepatic abscesses in hamsters. These results confirm that EhRabB is involved in phagocytosis and virulence of E. histolytica.
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