Guide general de reference d'images histologiques (450 images d'une quarantaine d'esp... more Guide general de reference d'images histologiques (450 images d'une quarantaine d'especes) dediees aux poissons pouvant apporter une reelle contribution dans le domaine de l’histologie normale, non pathologique, de ces animaux, domaine pour lequel la litterature de synthese est pauvre. Les pages et documents iconographiques realises ont pour but d'etre un guide general de reference destine a un large public disposant de connaissances de base ainsi que sur l'anatomie des poissons. Cet atlas peut donc s'adresser a des biologistes, histo(ichtyo)logistes et veterinaires en quete de renseignements precis, mais aussi a des etudiant(e)s et chercheurs travaillant dans les institutions academiques, ou encore a des aquariophiles, pisciculteurs, pecheurs,, amateurs d'"art et belles couleurs" et tout autre personne dont le « poisson » fait partie des centres d’interet.
Second generation rats depleted in long-chain polyunsaturated omega3 fatty acids are currently us... more Second generation rats depleted in long-chain polyunsaturated omega3 fatty acids are currently used as an animal model for the insufficient dietary supply of such fatty acids often prevailing in Western populations. The present study deals mainly with the effects of a novel medium-chain triglyceride: fish oil emulsion (MCT:FO), as compared to a control medium-chain triglyceride:olive oil emulsion (MCT: OO), administered as an intravenous bolus to the omega3-depleted rats 60-120 min before sacrifice upon selected biochemical and biophysical variables. The major findings consisted of a severe decrease of the omega3 fatty acid content of liver lipids in non-injected omega3-depleted rats and its partial correction after injection of the MCT:FO emulsion. The omega3-depleted rats also displayed liver steatosis, increased incorporation of long-chain polyunsaturated omega6 fatty acids in liver phospholipids and increased activity of liver Delta9-desaturase. As judged from the effects of oua...
This study evaluates the use of two fluorescein-labelled (FITC) plant lectins, Pisum sativum (edi... more This study evaluates the use of two fluorescein-labelled (FITC) plant lectins, Pisum sativum (edible pea) agglutinin (PSA) and Arachis hypogaea (peanut) agglutinin (PNA), in order to determine the most accurate and reliable method to experimentally detect and assess the acrosome reaction in mouse spermatozoa. PNA-FITC labelling was restricted to the acrosome and was not influenced by the fixation procedure; either absolute methanol or paraformaldehyde. In contrast, PSA-FITC not only labelled the acrosome, but also the whole head and the flagellum. This aspect was especially marked after methanol fixation. The cytoplasmic droplet, when present, was also stained by PSA-FITC. Incubation with the calcium ionophore ionomycin induced a concentration and time-dependent increase in the number of acrosome reactions. Compared to spotted preparations, smear samples exhibited a high proportion of spermatozoa with damaged acrosome. In conclusion, PNA-FITC labelling was more accurate than PSA-FIT...
This study aims to investigate whether the immunohistochemical expression of galectin-8 could be ... more This study aims to investigate whether the immunohistochemical expression of galectin-8 could be used as a diagnostic marker in tumor tissues of various histogenetic origins including specimens from epithelial (n=145), mesenchymatous (n=16), adipous (n=10) and central and peripheral nervous system (n=25) tissue, and 4 mesotheliomas. Immunohistochemical reactions were carried out with a polyclonal anti-galectin-8 antibody and histological slides from tissues derived from the files of the Laboratory of Anatomopathology of University Erasmus Hospital, Brussels. Formalin-fixed paraffin-embedded tissues of 45 normal cases as well as 41 benign and 114 malignant tumors were studied. Marked decreases in immunohistochemical galectin-8 expression were observed in colon (p=0.001), pancreas (p=0.007), liver (p=0.0008), skin (p=0.002) and larynx (p=0.02) tissue when comparing malignant tissue to normal tissue and/or benign tumors. The reverse relationship was observed for breast tissue (p=0.007)...
All current techniques (autoradiography excepted) for the detection of nuclear or cytosol steroid... more All current techniques (autoradiography excepted) for the detection of nuclear or cytosol steroid hormone receptors require homogenization of tissue, and thereby sophisticated equipment not available at all centers. Several methods have been developed to trace estrogen (ER) and progesterone receptors (PgR) under the microscope (see [2] for review). In this regard the ligands employed so far consist of: 1. Steroid hormones linked to a carrier molecule (bovine serum albumin) which is labeled with fluorescent dyes (∼2 × 10-4 M effective bound steroids) [10, 12] 2. Fluorescein-labeled estrogen molecule, without a protein carrier (∼ 10-6 M bound steroid) [5, 14]
JNCI: Journal of the National Cancer Institute, 1985
The effects of a single 17 beta-estradiol (E2) injection on cell proliferation were studied in 3 ... more The effects of a single 17 beta-estradiol (E2) injection on cell proliferation were studied in 3 groups of 30 mice transplanted with the MXT ovary-dependent mammary tumor. In group A, all animals were castrated prior to tumor implantation; groups B and C had intact ovaries at the time of transplantation, but group B was left intact throughout the experiment, while group C underwent castration 4 weeks later. On day 40 in groups B and C and on day 80 in group A, in which tumor development was significantly delayed, the same procedure for testing the effects of E2 was applied: Ten controls received 0.1 ml saline ip and were killed on the next day; 4 lots of 5 mice received 0.25 micrograms E2 ip and were killed one by one at 12-hour intervals. Exactly 1 hour prior to sacrifice, each animal received 25 microCi [methyl-3H]thymidine ip. Histologic sections of tumors and uteri were processed for autoradiography, and nuclear thymidine (dThd) labeling indices (LI) were determined. All tumors of group A grafted under unfavorable hormonal conditions were poorly differentiated, and E2 injection induced no appreciable changes in their dThd LI. Tumors B and C were well-differentiated adenocarcinomas, in which E2 induced significant modifications of cell proliferation. In group B, complex changes in dThd LI occurred in tumors as well as in uteri, probably due to interferences with the ovarian hormonal production. In group C, E2 produced a marked rise in dThd LI in tumors, lasting from the 12th to the 36th hour after its injection. Stimulation was maximum at the 24th hour, representing a 2.8-fold increase over mean basal dThd LI. It is concluded that the presence of an intact ovarian function at the time of transplantation is critical for maintaining the properties of hormone dependence in MXT tumors. In mice castrated after tumor implantation, a single E2 injection induces a marked and partially synchronous proliferation of neoplastic cells. The hypothesis that such hormonal manipulation might amplify the killing effect of cell cycle- or phase-specific cytotoxic drugs could be adequately tested with this model.
With the use of an in vivo tritiated thymidine [( 3H]dThd) nuclear labeling followed by autoradio... more With the use of an in vivo tritiated thymidine [( 3H]dThd) nuclear labeling followed by autoradiography, the effects at different times before sacrifice of prolactin (PRL) and/or 17 beta-estradiol (E2) were studied in C57BL X DBA/2f)F1 mice given transplants of the MXT hormone-sensitive mammary tumor whose growth was previously shown to be influenced by E2 and/or progesterone. Uteri were chosen as controls for the methodology. Experiments were conducted on ovariectomized mice submitted to endocrine manipulation to achieve plasma PRL modifications. In addition to E2, the proliferation of cancer cells, assessed by the measurement of thymidine labeling indices (TLIs), was demonstrated to be enhanced by ovine prolactin (oPRL) and Sulpiride and strongly slowed down by castration and 2-bromo-alpha-ergokryptin treatment, thus emphasizing the great importance of PRL in mammary cancer development. Moreover, a pulse of 1 mg oPRL/animal produced a marked TLI rise in tumors, lasting from the 6th to the 48th hour after its injection and reaching a maximum at 24 hours. PRL had no proliferative effect on the uterine luminal epithelium. When PRL and E2 were injected concomitantly, the profile of stimulation was quite similar to that obtained with E2 alone; i.e., a maximum stimulation was observed at the 24th and 36th hours after hormonal pulse. From these data it is concluded that, in spayed mice, not only E2 but also PRL is of major importance leading to enhanced proliferation of MXT mammary neoplastic cells. Further investigations are needed to throw light on the cellular events presiding over the action of PRL and E2 at the cancer cell level.
A series of 41 nasal polyps (23 single and 18 massive) and 6 normal nasal mucosa specimens was gl... more A series of 41 nasal polyps (23 single and 18 massive) and 6 normal nasal mucosa specimens was glycohistochemically investigated. Five plant lectins were used, ie, the peanut agglutinin (PNA), the wheat germ agglutinin (WGA), the gorse seed agglutinin (UEA-I), the Maackia amurensis agglutinin (MAA), and the elderberry bark agglutinin (SNA). A neoglycoconjugate and 2 animal lectins (CL-14 and CL-16) were also used. Three quantitative features were calculated by means of computer-assisted microscopy: the percentage of tissue area specifically stained by the histochemical probe, the staining intensity, and the heterogeneity level of the staining distribution. The results show that with respect to sialic acid-glycoprotein binding characteristics as determined by SNA, MAA, and WGA probes, the normal nasal mucosa differed markedly (p < .00001) from the polyposal one. The single nasal polyps exhibited glycohistochemical characteristics that differed markedly (p = .0004) from those exhib...
European Journal of Cancer and Clinical Oncology, 1986
The MXT transplantable mammary tumor is an ovarian sensitive carcinoma of the B6D2F1 mouse strain... more The MXT transplantable mammary tumor is an ovarian sensitive carcinoma of the B6D2F1 mouse strain. The present work documents the mitogenic effect of a single near-physiological dose (pulse) of estradiol (E2) in castrated mice bearing MXT neoplasms 2-7 weeks after their inoculation. Histological sections of tumors and uteri (as control E2 target organs) were processed and nuclear thymidine labeling indices (TLI) were determined by autoradiography. Tumor estrogen receptors contents were evaluated in parallel. The results indicate that the MXT tumor keeps a constant sensitivity to E2 6 weeks after implantation.
Guide general de reference d'images histologiques (450 images d'une quarantaine d'esp... more Guide general de reference d'images histologiques (450 images d'une quarantaine d'especes) dediees aux poissons pouvant apporter une reelle contribution dans le domaine de l’histologie normale, non pathologique, de ces animaux, domaine pour lequel la litterature de synthese est pauvre. Les pages et documents iconographiques realises ont pour but d'etre un guide general de reference destine a un large public disposant de connaissances de base ainsi que sur l'anatomie des poissons. Cet atlas peut donc s'adresser a des biologistes, histo(ichtyo)logistes et veterinaires en quete de renseignements precis, mais aussi a des etudiant(e)s et chercheurs travaillant dans les institutions academiques, ou encore a des aquariophiles, pisciculteurs, pecheurs,, amateurs d'"art et belles couleurs" et tout autre personne dont le « poisson » fait partie des centres d’interet.
Second generation rats depleted in long-chain polyunsaturated omega3 fatty acids are currently us... more Second generation rats depleted in long-chain polyunsaturated omega3 fatty acids are currently used as an animal model for the insufficient dietary supply of such fatty acids often prevailing in Western populations. The present study deals mainly with the effects of a novel medium-chain triglyceride: fish oil emulsion (MCT:FO), as compared to a control medium-chain triglyceride:olive oil emulsion (MCT: OO), administered as an intravenous bolus to the omega3-depleted rats 60-120 min before sacrifice upon selected biochemical and biophysical variables. The major findings consisted of a severe decrease of the omega3 fatty acid content of liver lipids in non-injected omega3-depleted rats and its partial correction after injection of the MCT:FO emulsion. The omega3-depleted rats also displayed liver steatosis, increased incorporation of long-chain polyunsaturated omega6 fatty acids in liver phospholipids and increased activity of liver Delta9-desaturase. As judged from the effects of oua...
This study evaluates the use of two fluorescein-labelled (FITC) plant lectins, Pisum sativum (edi... more This study evaluates the use of two fluorescein-labelled (FITC) plant lectins, Pisum sativum (edible pea) agglutinin (PSA) and Arachis hypogaea (peanut) agglutinin (PNA), in order to determine the most accurate and reliable method to experimentally detect and assess the acrosome reaction in mouse spermatozoa. PNA-FITC labelling was restricted to the acrosome and was not influenced by the fixation procedure; either absolute methanol or paraformaldehyde. In contrast, PSA-FITC not only labelled the acrosome, but also the whole head and the flagellum. This aspect was especially marked after methanol fixation. The cytoplasmic droplet, when present, was also stained by PSA-FITC. Incubation with the calcium ionophore ionomycin induced a concentration and time-dependent increase in the number of acrosome reactions. Compared to spotted preparations, smear samples exhibited a high proportion of spermatozoa with damaged acrosome. In conclusion, PNA-FITC labelling was more accurate than PSA-FIT...
This study aims to investigate whether the immunohistochemical expression of galectin-8 could be ... more This study aims to investigate whether the immunohistochemical expression of galectin-8 could be used as a diagnostic marker in tumor tissues of various histogenetic origins including specimens from epithelial (n=145), mesenchymatous (n=16), adipous (n=10) and central and peripheral nervous system (n=25) tissue, and 4 mesotheliomas. Immunohistochemical reactions were carried out with a polyclonal anti-galectin-8 antibody and histological slides from tissues derived from the files of the Laboratory of Anatomopathology of University Erasmus Hospital, Brussels. Formalin-fixed paraffin-embedded tissues of 45 normal cases as well as 41 benign and 114 malignant tumors were studied. Marked decreases in immunohistochemical galectin-8 expression were observed in colon (p=0.001), pancreas (p=0.007), liver (p=0.0008), skin (p=0.002) and larynx (p=0.02) tissue when comparing malignant tissue to normal tissue and/or benign tumors. The reverse relationship was observed for breast tissue (p=0.007)...
All current techniques (autoradiography excepted) for the detection of nuclear or cytosol steroid... more All current techniques (autoradiography excepted) for the detection of nuclear or cytosol steroid hormone receptors require homogenization of tissue, and thereby sophisticated equipment not available at all centers. Several methods have been developed to trace estrogen (ER) and progesterone receptors (PgR) under the microscope (see [2] for review). In this regard the ligands employed so far consist of: 1. Steroid hormones linked to a carrier molecule (bovine serum albumin) which is labeled with fluorescent dyes (∼2 × 10-4 M effective bound steroids) [10, 12] 2. Fluorescein-labeled estrogen molecule, without a protein carrier (∼ 10-6 M bound steroid) [5, 14]
JNCI: Journal of the National Cancer Institute, 1985
The effects of a single 17 beta-estradiol (E2) injection on cell proliferation were studied in 3 ... more The effects of a single 17 beta-estradiol (E2) injection on cell proliferation were studied in 3 groups of 30 mice transplanted with the MXT ovary-dependent mammary tumor. In group A, all animals were castrated prior to tumor implantation; groups B and C had intact ovaries at the time of transplantation, but group B was left intact throughout the experiment, while group C underwent castration 4 weeks later. On day 40 in groups B and C and on day 80 in group A, in which tumor development was significantly delayed, the same procedure for testing the effects of E2 was applied: Ten controls received 0.1 ml saline ip and were killed on the next day; 4 lots of 5 mice received 0.25 micrograms E2 ip and were killed one by one at 12-hour intervals. Exactly 1 hour prior to sacrifice, each animal received 25 microCi [methyl-3H]thymidine ip. Histologic sections of tumors and uteri were processed for autoradiography, and nuclear thymidine (dThd) labeling indices (LI) were determined. All tumors of group A grafted under unfavorable hormonal conditions were poorly differentiated, and E2 injection induced no appreciable changes in their dThd LI. Tumors B and C were well-differentiated adenocarcinomas, in which E2 induced significant modifications of cell proliferation. In group B, complex changes in dThd LI occurred in tumors as well as in uteri, probably due to interferences with the ovarian hormonal production. In group C, E2 produced a marked rise in dThd LI in tumors, lasting from the 12th to the 36th hour after its injection. Stimulation was maximum at the 24th hour, representing a 2.8-fold increase over mean basal dThd LI. It is concluded that the presence of an intact ovarian function at the time of transplantation is critical for maintaining the properties of hormone dependence in MXT tumors. In mice castrated after tumor implantation, a single E2 injection induces a marked and partially synchronous proliferation of neoplastic cells. The hypothesis that such hormonal manipulation might amplify the killing effect of cell cycle- or phase-specific cytotoxic drugs could be adequately tested with this model.
With the use of an in vivo tritiated thymidine [( 3H]dThd) nuclear labeling followed by autoradio... more With the use of an in vivo tritiated thymidine [( 3H]dThd) nuclear labeling followed by autoradiography, the effects at different times before sacrifice of prolactin (PRL) and/or 17 beta-estradiol (E2) were studied in C57BL X DBA/2f)F1 mice given transplants of the MXT hormone-sensitive mammary tumor whose growth was previously shown to be influenced by E2 and/or progesterone. Uteri were chosen as controls for the methodology. Experiments were conducted on ovariectomized mice submitted to endocrine manipulation to achieve plasma PRL modifications. In addition to E2, the proliferation of cancer cells, assessed by the measurement of thymidine labeling indices (TLIs), was demonstrated to be enhanced by ovine prolactin (oPRL) and Sulpiride and strongly slowed down by castration and 2-bromo-alpha-ergokryptin treatment, thus emphasizing the great importance of PRL in mammary cancer development. Moreover, a pulse of 1 mg oPRL/animal produced a marked TLI rise in tumors, lasting from the 6th to the 48th hour after its injection and reaching a maximum at 24 hours. PRL had no proliferative effect on the uterine luminal epithelium. When PRL and E2 were injected concomitantly, the profile of stimulation was quite similar to that obtained with E2 alone; i.e., a maximum stimulation was observed at the 24th and 36th hours after hormonal pulse. From these data it is concluded that, in spayed mice, not only E2 but also PRL is of major importance leading to enhanced proliferation of MXT mammary neoplastic cells. Further investigations are needed to throw light on the cellular events presiding over the action of PRL and E2 at the cancer cell level.
A series of 41 nasal polyps (23 single and 18 massive) and 6 normal nasal mucosa specimens was gl... more A series of 41 nasal polyps (23 single and 18 massive) and 6 normal nasal mucosa specimens was glycohistochemically investigated. Five plant lectins were used, ie, the peanut agglutinin (PNA), the wheat germ agglutinin (WGA), the gorse seed agglutinin (UEA-I), the Maackia amurensis agglutinin (MAA), and the elderberry bark agglutinin (SNA). A neoglycoconjugate and 2 animal lectins (CL-14 and CL-16) were also used. Three quantitative features were calculated by means of computer-assisted microscopy: the percentage of tissue area specifically stained by the histochemical probe, the staining intensity, and the heterogeneity level of the staining distribution. The results show that with respect to sialic acid-glycoprotein binding characteristics as determined by SNA, MAA, and WGA probes, the normal nasal mucosa differed markedly (p < .00001) from the polyposal one. The single nasal polyps exhibited glycohistochemical characteristics that differed markedly (p = .0004) from those exhib...
European Journal of Cancer and Clinical Oncology, 1986
The MXT transplantable mammary tumor is an ovarian sensitive carcinoma of the B6D2F1 mouse strain... more The MXT transplantable mammary tumor is an ovarian sensitive carcinoma of the B6D2F1 mouse strain. The present work documents the mitogenic effect of a single near-physiological dose (pulse) of estradiol (E2) in castrated mice bearing MXT neoplasms 2-7 weeks after their inoculation. Histological sections of tumors and uteri (as control E2 target organs) were processed and nuclear thymidine labeling indices (TLI) were determined by autoradiography. Tumor estrogen receptors contents were evaluated in parallel. The results indicate that the MXT tumor keeps a constant sensitivity to E2 6 weeks after implantation.
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Papers by Andre DANGUY