This retrospective real-world study investigated the clinical use of the sFlt-1 (soluble fms-like... more This retrospective real-world study investigated the clinical use of the sFlt-1 (soluble fms-like tyrosine kinase 1)/PlGF (placental growth factor) ratio alone or in combination with other clinical tests to predict an adverse maternal (maternal death, kidney failure, hemolysis elevated liver enzymes low platelets-syndrome, pulmonary edema, disseminated intravascular coagulation, cerebral hemorrhage, or eclampsia) or fetal (delivery before 34 weeks because of preeclampsia and/or intrauterine growth restriction, respiratory distress syndrome, necrotizing enterocolitis, intraventricular hemorrhage, placental abruption or intrauterine fetal death or neonatal death within 7 days post natum) pregnancy outcome in patients with signs and symptoms of preeclampsia. We evaluated the sFlt-1/PlGF-ratio cutoff values of 38 and 85 and evaluated its integration into a multimarker model. Of 1117 subjects, 322 (28.8%) developed an adverse fetal or maternal outcome. Patients with an adverse versus no ...
Endothelial dysfunction plays a major role in cardiovascular diseases and pulse amplitude tonomet... more Endothelial dysfunction plays a major role in cardiovascular diseases and pulse amplitude tonometry (PAT) offers a non-invasive way to assess endothelial dysfunction. However, data about the reliability of PAT in cardiovascular patient populations are scarce. Thus, we evaluated the test-retest reliability of PAT using the natural logarithmic transformed reactive hyperaemia index (LnRHI). Our cohort consisted of 91 patients (mean age: 65±9.7 years, 32% female), who were divided into four groups: those with heart failure with preserved ejection fraction (HFpEF) ( n=25), heart failure with reduced ejection fraction (HFrEF) ( n=22), diabetic nephropathy ( n=21), and arterial hypertension ( n=23). All subjects underwent two separate PAT measurements at a median interval of 7 days (range 4–14 days). LnRHI derived by PAT showed good reliability in subjects with diabetic nephropathy (intra-class correlation (ICC) = 0.863) and satisfactory reliability in patients with both HFpEF (ICC = 0.557...
Twin zygosity determinations can be performed with anthropologic, serologic and genetic markers; ... more Twin zygosity determinations can be performed with anthropologic, serologic and genetic markers; however, these methods are more than occasionally inefficient, often expensive and sometimes inaccurate. We used microsatellites as DNA markers and developed a largely automated, rapid and efficient method of determining zygosity. We used five highly polymorphic short tandem repeat loci, coamplified by polymerase chain reaction (PCR) using fluorescence-labeled primers. Thirty-six samples were simultaneously analyzed by electrophoresis and laser detection. The PCR products were sized by automated fragment analysis. We typed 132 pairs of monozygotic (MZ) and dizygotic (DZ) twins. With five markers, the probability that any twin pair was MZ if all markers were concordant was 99%. This method is a rapid and reliable approach to zygosity detection.
There are numerous data indicating a significant role of the sympathoadrenal system and the renin... more There are numerous data indicating a significant role of the sympathoadrenal system and the reninangiotensin- aldosterone system in the regulation of blood pressure and the pathogenesis of essential hypertension. However, the genetic background of essential hypertension remains unclear. To determine the effects of genetic factors on selected indicators of the activity of the sympathoadrenal system and the renin-angiotensin-aldosterone system in twins. We studied 39 monozygotic twin pairs (age 33+/-7 years) and 37 same-gender dizygotic twin pairs (age 36+/-7 years). We measured blood and urine adrenaline (A), noradrenaline (NA), dopamine (DA) and aldosterone (ALD) levels, as well as plasma renin activity (PRA) and serum angiotensin-converting enzyme (ACE) activity. Parameters of the genetic models for age- and gender-adjusted data were estimated by model fitting and path analysis technique using LISREL 8. The effects of genetic factors on the variability of blood and urine catecholam...
Proceedings - 11th Koli Calling International Conference on Computing Education Research, Koli Calling'11, 2011
ABSTRACT Code reading, although an integral part of program comprehension, is rarely reflected. I... more ABSTRACT Code reading, although an integral part of program comprehension, is rarely reflected. In this paper, we want to argue for a research approach and direction exploiting the potential that lies in the analysis of reading processes. Based on the vast experience compiled in psychology and some studies in computing, eye tracking and think aloud were elaborated as a viable research instrument for code reading studies. We conducted a feasibility study, designed to examine the actual process of code reading as the sensory starting point of comprehension. Computational and statistical tools were developed to facilitate data capture and analysis for eye tracking experiments. Results do not just provide proof of concept but already emphasize differences between reading natural language text and source code, as well as a distinct attention allocation within different code elements like keywords and operators. In conclusion we suggest a combination of theory-driven selected stimuli material, a carefully designed procedure of eye tracking, complemented with suitable post-tests on comprehension as well as retrospective think aloud in order to obtain additional information on the linking process between perception and comprehension. As an addition to other research approaches this should most certainly help us to improve our knowledge of comprehension within an educational research framework.
The radiosensitivity of human lymphocytes measured using a G0- or G2-assay has been linked with a... more The radiosensitivity of human lymphocytes measured using a G0- or G2-assay has been linked with an individual's risk of developing normal tissue complications following radiotherapy. This study was performed to increase basic knowledge of the genetics of the human radiation response, and chromosomal aberration induction in particular. The study was carried out with blood samples taken from 15 monozygotic twin pairs. G0-assay was performed for cells irradiated with 6 Gy counting only deletions and G2-assay for cells irradiated with 0.5 Gy scoring only chromatid breaks. The mean number of deletions measured at 6 Gy for all 30 samples using the G0-assay amounted to 2.96+/-0.37 (means+/-SD), which corresponds to a coefficient of variation (CV) of 13%. There is a highly significant intra-pair correlation for this number among twins (r(2)=0.911) demonstrating that this parameter is mostly determined by genetic factors. According to the mean number of deletions, a theoretical classification based on the definition < or = MV-SD as resistant, MV+/-SD as normal and > or = MV+SD as sensitive was made, identifying two pairs as sensitive or resistant, respectively, while nine were normal and two pairs are intermediate. For chromatid breaks measured at 0.5 Gy with the G2-assay the mean number was 1.35+/-0.42 (means+/-SD) corresponding to a CV of 31%. There was again a strong intra-pair correlation among twins with r(2)=0.837 showing that this sensitivity is also determined mostly by genetic factors. There was, however, no inter-assay correlation between the G0- and G2-sensitivity (r(2)=0.006) demonstrating that these two sensitivities depend on different genetic factors. The chromosomal radiosensitivity of lymphocytes as defined by G0- or G2-assay is largely determined by different genetic factors, which may allow the use of genetic profiling as an indicator of the respective individual radiosensitivity.
We examined a Turkish kindred with a unique form of autosomal dominant hypertension that cosegreg... more We examined a Turkish kindred with a unique form of autosomal dominant hypertension that cosegregates 100% with brachydactyly and maps to chromosome 12p. Affected adults were 10 to 15 cm shorter than unaffected people; however, their body mass index (27 kg/m 2 ) was not different. Blood pressure increased steeply with age in the affected people so that by age 40 years, they had a mean blood pressure of 140 mm Hg, compared with 92 mm Hg in unaffected individuals. Complete clinical, roentgenographic, and laboratory evaluation was performed in 6 subjects, including 24-hour blood pressure measurements and humoral determinations before and after volume expansion with 2 L normal saline over 4 hours followed by volume contraction on the following day with a 20-mmol sodium diet and 40 mg furosemide at 8 am , noon, and 4 pm . Two affected men aged 46 and 31 years; 3 affected women aged 40, 31, and 30 years; and 1 unaffected man aged 29 years were studied. Systolic pressures ranged from 170 t...
Coronary artery disease and disturbances of lipid levels are common in Arab countries. To assess ... more Coronary artery disease and disturbances of lipid levels are common in Arab countries. To assess homocysteine as a cardiovascular risk factor. We compared 133 men with angiographically documented coronary heart disease with 130 age-matched asymptomatic men. Cases had more hypertension and diabetes and higher levels of total cholesterol, low-density lipoprotein cholesterol, triglycerides, fibrinogen, and homocysteine than did controls. The homocysteine level distribution of cases was shifted toward higher concentrations (P < 0.05) compared with those in controls. When patients with one-vessel, two-vessel, and three-vessel disease were compared, only levels of fibrinogen and homocysteine were associated with the numbers of vessels involved. Homocysteine level was not correlated to fibrinogen and lipid concentrations. A multiple regression analysis revealed that only age (P = 0.06) and smoking (P = 0.04) were marginally related to homocysteine concentrations. Homocysteine concentrations in cases were significantly different than those in controls, even after adjustment for all covariates (P < 0.006). Hyperhomocysteinemia is independently associated with coronary artery disease in Arab men. Furthermore, fibrinogen concentrations are also an important risk factor for Arab men.
This retrospective real-world study investigated the clinical use of the sFlt-1 (soluble fms-like... more This retrospective real-world study investigated the clinical use of the sFlt-1 (soluble fms-like tyrosine kinase 1)/PlGF (placental growth factor) ratio alone or in combination with other clinical tests to predict an adverse maternal (maternal death, kidney failure, hemolysis elevated liver enzymes low platelets-syndrome, pulmonary edema, disseminated intravascular coagulation, cerebral hemorrhage, or eclampsia) or fetal (delivery before 34 weeks because of preeclampsia and/or intrauterine growth restriction, respiratory distress syndrome, necrotizing enterocolitis, intraventricular hemorrhage, placental abruption or intrauterine fetal death or neonatal death within 7 days post natum) pregnancy outcome in patients with signs and symptoms of preeclampsia. We evaluated the sFlt-1/PlGF-ratio cutoff values of 38 and 85 and evaluated its integration into a multimarker model. Of 1117 subjects, 322 (28.8%) developed an adverse fetal or maternal outcome. Patients with an adverse versus no ...
Endothelial dysfunction plays a major role in cardiovascular diseases and pulse amplitude tonomet... more Endothelial dysfunction plays a major role in cardiovascular diseases and pulse amplitude tonometry (PAT) offers a non-invasive way to assess endothelial dysfunction. However, data about the reliability of PAT in cardiovascular patient populations are scarce. Thus, we evaluated the test-retest reliability of PAT using the natural logarithmic transformed reactive hyperaemia index (LnRHI). Our cohort consisted of 91 patients (mean age: 65±9.7 years, 32% female), who were divided into four groups: those with heart failure with preserved ejection fraction (HFpEF) ( n=25), heart failure with reduced ejection fraction (HFrEF) ( n=22), diabetic nephropathy ( n=21), and arterial hypertension ( n=23). All subjects underwent two separate PAT measurements at a median interval of 7 days (range 4–14 days). LnRHI derived by PAT showed good reliability in subjects with diabetic nephropathy (intra-class correlation (ICC) = 0.863) and satisfactory reliability in patients with both HFpEF (ICC = 0.557...
Twin zygosity determinations can be performed with anthropologic, serologic and genetic markers; ... more Twin zygosity determinations can be performed with anthropologic, serologic and genetic markers; however, these methods are more than occasionally inefficient, often expensive and sometimes inaccurate. We used microsatellites as DNA markers and developed a largely automated, rapid and efficient method of determining zygosity. We used five highly polymorphic short tandem repeat loci, coamplified by polymerase chain reaction (PCR) using fluorescence-labeled primers. Thirty-six samples were simultaneously analyzed by electrophoresis and laser detection. The PCR products were sized by automated fragment analysis. We typed 132 pairs of monozygotic (MZ) and dizygotic (DZ) twins. With five markers, the probability that any twin pair was MZ if all markers were concordant was 99%. This method is a rapid and reliable approach to zygosity detection.
There are numerous data indicating a significant role of the sympathoadrenal system and the renin... more There are numerous data indicating a significant role of the sympathoadrenal system and the reninangiotensin- aldosterone system in the regulation of blood pressure and the pathogenesis of essential hypertension. However, the genetic background of essential hypertension remains unclear. To determine the effects of genetic factors on selected indicators of the activity of the sympathoadrenal system and the renin-angiotensin-aldosterone system in twins. We studied 39 monozygotic twin pairs (age 33+/-7 years) and 37 same-gender dizygotic twin pairs (age 36+/-7 years). We measured blood and urine adrenaline (A), noradrenaline (NA), dopamine (DA) and aldosterone (ALD) levels, as well as plasma renin activity (PRA) and serum angiotensin-converting enzyme (ACE) activity. Parameters of the genetic models for age- and gender-adjusted data were estimated by model fitting and path analysis technique using LISREL 8. The effects of genetic factors on the variability of blood and urine catecholam...
Proceedings - 11th Koli Calling International Conference on Computing Education Research, Koli Calling'11, 2011
ABSTRACT Code reading, although an integral part of program comprehension, is rarely reflected. I... more ABSTRACT Code reading, although an integral part of program comprehension, is rarely reflected. In this paper, we want to argue for a research approach and direction exploiting the potential that lies in the analysis of reading processes. Based on the vast experience compiled in psychology and some studies in computing, eye tracking and think aloud were elaborated as a viable research instrument for code reading studies. We conducted a feasibility study, designed to examine the actual process of code reading as the sensory starting point of comprehension. Computational and statistical tools were developed to facilitate data capture and analysis for eye tracking experiments. Results do not just provide proof of concept but already emphasize differences between reading natural language text and source code, as well as a distinct attention allocation within different code elements like keywords and operators. In conclusion we suggest a combination of theory-driven selected stimuli material, a carefully designed procedure of eye tracking, complemented with suitable post-tests on comprehension as well as retrospective think aloud in order to obtain additional information on the linking process between perception and comprehension. As an addition to other research approaches this should most certainly help us to improve our knowledge of comprehension within an educational research framework.
The radiosensitivity of human lymphocytes measured using a G0- or G2-assay has been linked with a... more The radiosensitivity of human lymphocytes measured using a G0- or G2-assay has been linked with an individual's risk of developing normal tissue complications following radiotherapy. This study was performed to increase basic knowledge of the genetics of the human radiation response, and chromosomal aberration induction in particular. The study was carried out with blood samples taken from 15 monozygotic twin pairs. G0-assay was performed for cells irradiated with 6 Gy counting only deletions and G2-assay for cells irradiated with 0.5 Gy scoring only chromatid breaks. The mean number of deletions measured at 6 Gy for all 30 samples using the G0-assay amounted to 2.96+/-0.37 (means+/-SD), which corresponds to a coefficient of variation (CV) of 13%. There is a highly significant intra-pair correlation for this number among twins (r(2)=0.911) demonstrating that this parameter is mostly determined by genetic factors. According to the mean number of deletions, a theoretical classification based on the definition < or = MV-SD as resistant, MV+/-SD as normal and > or = MV+SD as sensitive was made, identifying two pairs as sensitive or resistant, respectively, while nine were normal and two pairs are intermediate. For chromatid breaks measured at 0.5 Gy with the G2-assay the mean number was 1.35+/-0.42 (means+/-SD) corresponding to a CV of 31%. There was again a strong intra-pair correlation among twins with r(2)=0.837 showing that this sensitivity is also determined mostly by genetic factors. There was, however, no inter-assay correlation between the G0- and G2-sensitivity (r(2)=0.006) demonstrating that these two sensitivities depend on different genetic factors. The chromosomal radiosensitivity of lymphocytes as defined by G0- or G2-assay is largely determined by different genetic factors, which may allow the use of genetic profiling as an indicator of the respective individual radiosensitivity.
We examined a Turkish kindred with a unique form of autosomal dominant hypertension that cosegreg... more We examined a Turkish kindred with a unique form of autosomal dominant hypertension that cosegregates 100% with brachydactyly and maps to chromosome 12p. Affected adults were 10 to 15 cm shorter than unaffected people; however, their body mass index (27 kg/m 2 ) was not different. Blood pressure increased steeply with age in the affected people so that by age 40 years, they had a mean blood pressure of 140 mm Hg, compared with 92 mm Hg in unaffected individuals. Complete clinical, roentgenographic, and laboratory evaluation was performed in 6 subjects, including 24-hour blood pressure measurements and humoral determinations before and after volume expansion with 2 L normal saline over 4 hours followed by volume contraction on the following day with a 20-mmol sodium diet and 40 mg furosemide at 8 am , noon, and 4 pm . Two affected men aged 46 and 31 years; 3 affected women aged 40, 31, and 30 years; and 1 unaffected man aged 29 years were studied. Systolic pressures ranged from 170 t...
Coronary artery disease and disturbances of lipid levels are common in Arab countries. To assess ... more Coronary artery disease and disturbances of lipid levels are common in Arab countries. To assess homocysteine as a cardiovascular risk factor. We compared 133 men with angiographically documented coronary heart disease with 130 age-matched asymptomatic men. Cases had more hypertension and diabetes and higher levels of total cholesterol, low-density lipoprotein cholesterol, triglycerides, fibrinogen, and homocysteine than did controls. The homocysteine level distribution of cases was shifted toward higher concentrations (P < 0.05) compared with those in controls. When patients with one-vessel, two-vessel, and three-vessel disease were compared, only levels of fibrinogen and homocysteine were associated with the numbers of vessels involved. Homocysteine level was not correlated to fibrinogen and lipid concentrations. A multiple regression analysis revealed that only age (P = 0.06) and smoking (P = 0.04) were marginally related to homocysteine concentrations. Homocysteine concentrations in cases were significantly different than those in controls, even after adjustment for all covariates (P < 0.006). Hyperhomocysteinemia is independently associated with coronary artery disease in Arab men. Furthermore, fibrinogen concentrations are also an important risk factor for Arab men.
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Papers by Andreas Busjahn