Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP
Pneumonia é uma causa importante de morbidade e mortalidade em potros. Embora muitos organismos s... more Pneumonia é uma causa importante de morbidade e mortalidade em potros. Embora muitos organismos sejam associados com pneumonia em potros, o Rhodococcus equi é considerado um dos problemas mais importantes em criatórios do mundo todo. Este artigo de revisão discute os princípios gerais de etiopatologia, epidemiologia, imunologia, além de recomendações específicas para o diagnóstico, o tratamento, a prevenção e o controle da pneumonia causada pelo R. equi.
The poisonous plant Baccharis coridifolia causes necrosis in lymphoid tissues and the gastrointes... more The poisonous plant Baccharis coridifolia causes necrosis in lymphoid tissues and the gastrointestinal tract of cattle, horses, sheep and rabbits. An experimental poisoning of mice was undertaken to establish an experimental model in a laboratory animal specie. A single 5 to 8-g/kg dose of a suspension of the plant was administered by gavage to 11 mice. To 3 other control mice, the same volume of water was administered. Plant-dosed mice manifested clinical effects after 12 h: tachipnea, trembles, dehydration and prostration. Most of the dosed mice died 14 to 33 h after plant administration--3 survived for 12 d. Six mice had remarkable necrosis of the germinative center of secondary follicles in lymph nodes and spleen; 3 mice had necrosis of lymphoid tissues in intestine and thymus. Mice reproduce most of the lesions observed in naturally poisoned cattle and the use of this specie as an experimental model is valid.
No presente estudo, avaliou-se a eficácia do emprego do peritônio bovino, conservado em glicerina... more No presente estudo, avaliou-se a eficácia do emprego do peritônio bovino, conservado em glicerina a 98%, no reparo de lesões induzidas no tendão calcâneo (TC) de cães, quando um fragmento de aproximadamente 1cm do TC foi excisado e o espaço resultante preenchido por um fragmento de peritônio. Foram utilizados 21 cães, pesando entre 10 e 15kg, divididos em 7 grupos de 3, sacrificados aos 02, 07, 15, 30, 60, 90 e 120 dias de pós-operatório. Analisaram-se os aspectos clínico-cirúrgicos referentes à recuperação funcional motora, bem como, a integração do peritônio com o tecido tendíneo mediante avaliação macroscópica, por microscopia óptica e por microscopia eletrônica de varredura. Clinicamente, verificou-se que, por volta do 55º dia de pós-operatório, os animais já apresentavam deambulação normal e que o…
In three experiments, three different hydrated sodium calcium aluminosilicates (HSCAS) were incor... more In three experiments, three different hydrated sodium calcium aluminosilicates (HSCAS) were incorporated into chick diets (.5%) containing either 0 or 5.0 (Experiments 1 and 2) or 0 or 2.5 (Experiment 3) mg/kg aflatoxin (AF). Male broiler chicks consumed their respective diets and water ad libitum from 1 to 21 days of age. When compared with controls, body weights in chicks receiving 5.0 mg AF/kg were reduced by 214 g in Experiment 1 and 220 g in Experiment 2. The addition of .5% of the HSCAS compounds significantly diminished the growth inhibitory effects caused by AF by 39 to 68% in Experiment 1, by 46 to 88% in Experiment 2, and by 38 to 90% in Experiment 3. The increases in relative organ weights and the decreases in serum biochemical values caused by AF were significantly diminished to differing degrees by all three of the HSCAS compounds. These data demonstrate that these specific HSCAS compounds can be protective against the effects of AF in young growing broilers and further emphasizes the fact that all silicate-type sorbents are not equal in their ability to protect against aflatoxicosis. It also seems possible to specially process compounds to increase their efficacy for protection against the toxicity of AF.
The purpose was to determine the immunophenotype of canine lymphomas (CLs) classified according t... more The purpose was to determine the immunophenotype of canine lymphomas (CLs) classified according to the WHO nomenclature for domestic animals, and to relate these findings to the immunoexpression of p53 protein. Lymphomas were immunophenotyped with antibodies to CD79a, CD3, and p53 protein, suitable for paraffin wax-embedded tissue sections. Of 55 cases, 40 (72.7%) were of the B-cell phenotype, 12 (21.8%) of the T-cell phenotype, and three (5.4%) were non-B-non-T lymphomas. Of the 40 B-cell lymphomas, 31 were of the large B-cell type, six were lymphoplasmacytic, one lymphocytic, one follicular (grade II) and one unclassified low-grade. One of the peripheral T-cell lymphomas was morphologically consistent with anaplastic large cell lymphoma. Immunolabelling for p53 occurred in 24 B-cell and seven T-cell lymphomas. Lymphomas with high grade histology showed a significantly increased frequency of p53 positivity (P = 0.01). Positivity for p53 (more than 10% positive cells) tended to be associated with the T-cell phenotype (P = 0.06). Mean patient age was significantly higher in p53-positive cases (P = 0.02). These data are comparable with findings in human lymphomas.
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2001
Relataram-se oito casos de dermovilite exsudativa vegetante crônica em seis eqüinos de diferentes... more Relataram-se oito casos de dermovilite exsudativa vegetante crônica em seis eqüinos de diferentes raças, com idades entre 14 meses e 19 anos. A lesão iniciava-se pelo aparecimento de um tecido podofiloso infiltrativo na região da ranilha e da sola do casco, caracterizado por crescimento rápido e desordenado, de aspecto papiliforme, de coloração esbranquiçada na raiz e escura nas pontas, com secreção necrótica de odor extremamente fétido. Microscopicamente, observou-se a presença de exuberante tecido epidérmico proliferativo, entremeado por escasso tecido conjuntivo. Bactérias gram negativas, associadas à má higiene e umidade, estão incluídas entre os fatores etiológicos, porém prescindem de confirmação científica. Os eqüinos foram divididos em dois grupos de tratamento. No primeiro grupo, constituído de três éguas jovens e um potro, portando lesões em apenas um membro, realizou-se inicialmente remoção cirúrgica da massa invasiva, seguida de cauterização das bordas restantes e poster...
Anticoccidial agents are commonly used in the poultry industry. One of them, Monensin, is widely ... more Anticoccidial agents are commonly used in the poultry industry. One of them, Monensin, is widely used, and in toxic levels can induce lesions in cardiac and skeletal muscle. Roxarsone can be used in addition to Monensin, increasing its efficacy. Peripheral neuropathy has been shown in chickens that were fed roxarsone supplemented diets and subjected to stress. This paper shows the effects of Monensin in association with Roxarsone in broiler chicken to develop an experimental model that concerns gross and microscopic study of lesions in peripheral nerves and cardiac and skeletal muscle. Broiler ckickens are select due to fast growing and developing. 360 chickens were fed either Monensin and Roxarsona for a period of thirty five days with two regimens (Monensin 300 ppm /Roxarsone 80 ppm ; Monensin 300 ppm /Roxarsone 0 ppm; Monensin 150 ppm /Roxarsone 80 ppm; Monensin 150 ppm /Roxarsone 0 ppm; Monensin 0 ppm /Roxarsone 80ppm and Monensin 0 ppm /Roxarsone 0ppm). After this period, birds were euthanatized and muscles and nerves were removed to submit to microscopic examination. The histopathologic findings showed necrosis and myodegeneration in cardiac and skeletal muscle, and macrophages between the fibers. Microscopic lesions were observed in peripheral nerves and cardiac and skeletal muscles consisting in degeneration of and infiltration of inflammatory cells. The use of the association of these drugs in broilers showed to be adequate as a model for nerve and muscle lesions.109
ABSTRACT Chronic hypertrophic pododermatitis cases in six horses of different breeds, aging 14 mo... more ABSTRACT Chronic hypertrophic pododermatitis cases in six horses of different breeds, aging 14 months to 19 years are described. The lesion begun with a infiltrative tissue in the frog and sole regions of the hoof, characterized by a fast and disorganized growth, with a papillary aspect, white colored in the roots and dark on the extremity, with a necrotic secretion and an extremely fetid odor. Microscopically, an exuberant epidermic proliferative tissue was observed, intermingled with little connective tissue. The horses were divided into two treatment groups. In the first group, including three young mares and a foal, showing lesions in only one limb, a surgical resection of the invasive mass was performed, followed by cauterization of the remaining edges and subsequent daily local application of antiseptic substances. In three of these horses, recurrence of the initial lesion occurred, with fast growth of hyperplasic tissue, affecting almost all the frog and half of the sole. Two horses developed contraction deformities of the hoof. In the second group, one male and one female, each with lesions in two limbs, after surgical debridement of the tissue, the animals received daily applications of picric acid 5%, associated to local use of oxitetracyclin. Although one of these cases required a second surgical intervention for removal of the mass, the horses showed after a period of two to three months total absence of the infiltrative tissue. The use of local picric acid 5% and oxitetracyclin associated to previous surgical debridement showed to be more efficient than the use of antiseptic substances in the treatment of chronic hypertrophic pododermatitis.
The number of cases of Bovine Spongiform Encephalopathy (BSE) in the United Kingdom (UK) and Euro... more The number of cases of Bovine Spongiform Encephalopathy (BSE) in the United Kingdom (UK) and Europe has decreased during the last years. The disease is designated as Classical BSE (C-BSE) once the prion protein involved is very constant and has genetic identity. On the other hand, atypical cases have occurred in several countries, apparently without any relationship with contaminated feeding. Atypical cases, H or L-BSE can have involvement with the etiology of the known C-BSE, first diagnosed in the UK. With the control of C-BSE based on rigid control of feeding, the occurrence of atypical BSE may predominate in the future. In this hypothesis atypical BSE, probably a spontaneous encephalopathy of cattle, will be considered as Sporadic BSE in contrast with the C-BSE that could be nominated UK-BSE.
Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP
Pneumonia é uma causa importante de morbidade e mortalidade em potros. Embora muitos organismos s... more Pneumonia é uma causa importante de morbidade e mortalidade em potros. Embora muitos organismos sejam associados com pneumonia em potros, o Rhodococcus equi é considerado um dos problemas mais importantes em criatórios do mundo todo. Este artigo de revisão discute os princípios gerais de etiopatologia, epidemiologia, imunologia, além de recomendações específicas para o diagnóstico, o tratamento, a prevenção e o controle da pneumonia causada pelo R. equi.
The poisonous plant Baccharis coridifolia causes necrosis in lymphoid tissues and the gastrointes... more The poisonous plant Baccharis coridifolia causes necrosis in lymphoid tissues and the gastrointestinal tract of cattle, horses, sheep and rabbits. An experimental poisoning of mice was undertaken to establish an experimental model in a laboratory animal specie. A single 5 to 8-g/kg dose of a suspension of the plant was administered by gavage to 11 mice. To 3 other control mice, the same volume of water was administered. Plant-dosed mice manifested clinical effects after 12 h: tachipnea, trembles, dehydration and prostration. Most of the dosed mice died 14 to 33 h after plant administration--3 survived for 12 d. Six mice had remarkable necrosis of the germinative center of secondary follicles in lymph nodes and spleen; 3 mice had necrosis of lymphoid tissues in intestine and thymus. Mice reproduce most of the lesions observed in naturally poisoned cattle and the use of this specie as an experimental model is valid.
No presente estudo, avaliou-se a eficácia do emprego do peritônio bovino, conservado em glicerina... more No presente estudo, avaliou-se a eficácia do emprego do peritônio bovino, conservado em glicerina a 98%, no reparo de lesões induzidas no tendão calcâneo (TC) de cães, quando um fragmento de aproximadamente 1cm do TC foi excisado e o espaço resultante preenchido por um fragmento de peritônio. Foram utilizados 21 cães, pesando entre 10 e 15kg, divididos em 7 grupos de 3, sacrificados aos 02, 07, 15, 30, 60, 90 e 120 dias de pós-operatório. Analisaram-se os aspectos clínico-cirúrgicos referentes à recuperação funcional motora, bem como, a integração do peritônio com o tecido tendíneo mediante avaliação macroscópica, por microscopia óptica e por microscopia eletrônica de varredura. Clinicamente, verificou-se que, por volta do 55º dia de pós-operatório, os animais já apresentavam deambulação normal e que o…
In three experiments, three different hydrated sodium calcium aluminosilicates (HSCAS) were incor... more In three experiments, three different hydrated sodium calcium aluminosilicates (HSCAS) were incorporated into chick diets (.5%) containing either 0 or 5.0 (Experiments 1 and 2) or 0 or 2.5 (Experiment 3) mg/kg aflatoxin (AF). Male broiler chicks consumed their respective diets and water ad libitum from 1 to 21 days of age. When compared with controls, body weights in chicks receiving 5.0 mg AF/kg were reduced by 214 g in Experiment 1 and 220 g in Experiment 2. The addition of .5% of the HSCAS compounds significantly diminished the growth inhibitory effects caused by AF by 39 to 68% in Experiment 1, by 46 to 88% in Experiment 2, and by 38 to 90% in Experiment 3. The increases in relative organ weights and the decreases in serum biochemical values caused by AF were significantly diminished to differing degrees by all three of the HSCAS compounds. These data demonstrate that these specific HSCAS compounds can be protective against the effects of AF in young growing broilers and further emphasizes the fact that all silicate-type sorbents are not equal in their ability to protect against aflatoxicosis. It also seems possible to specially process compounds to increase their efficacy for protection against the toxicity of AF.
The purpose was to determine the immunophenotype of canine lymphomas (CLs) classified according t... more The purpose was to determine the immunophenotype of canine lymphomas (CLs) classified according to the WHO nomenclature for domestic animals, and to relate these findings to the immunoexpression of p53 protein. Lymphomas were immunophenotyped with antibodies to CD79a, CD3, and p53 protein, suitable for paraffin wax-embedded tissue sections. Of 55 cases, 40 (72.7%) were of the B-cell phenotype, 12 (21.8%) of the T-cell phenotype, and three (5.4%) were non-B-non-T lymphomas. Of the 40 B-cell lymphomas, 31 were of the large B-cell type, six were lymphoplasmacytic, one lymphocytic, one follicular (grade II) and one unclassified low-grade. One of the peripheral T-cell lymphomas was morphologically consistent with anaplastic large cell lymphoma. Immunolabelling for p53 occurred in 24 B-cell and seven T-cell lymphomas. Lymphomas with high grade histology showed a significantly increased frequency of p53 positivity (P = 0.01). Positivity for p53 (more than 10% positive cells) tended to be associated with the T-cell phenotype (P = 0.06). Mean patient age was significantly higher in p53-positive cases (P = 0.02). These data are comparable with findings in human lymphomas.
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2001
Relataram-se oito casos de dermovilite exsudativa vegetante crônica em seis eqüinos de diferentes... more Relataram-se oito casos de dermovilite exsudativa vegetante crônica em seis eqüinos de diferentes raças, com idades entre 14 meses e 19 anos. A lesão iniciava-se pelo aparecimento de um tecido podofiloso infiltrativo na região da ranilha e da sola do casco, caracterizado por crescimento rápido e desordenado, de aspecto papiliforme, de coloração esbranquiçada na raiz e escura nas pontas, com secreção necrótica de odor extremamente fétido. Microscopicamente, observou-se a presença de exuberante tecido epidérmico proliferativo, entremeado por escasso tecido conjuntivo. Bactérias gram negativas, associadas à má higiene e umidade, estão incluídas entre os fatores etiológicos, porém prescindem de confirmação científica. Os eqüinos foram divididos em dois grupos de tratamento. No primeiro grupo, constituído de três éguas jovens e um potro, portando lesões em apenas um membro, realizou-se inicialmente remoção cirúrgica da massa invasiva, seguida de cauterização das bordas restantes e poster...
Anticoccidial agents are commonly used in the poultry industry. One of them, Monensin, is widely ... more Anticoccidial agents are commonly used in the poultry industry. One of them, Monensin, is widely used, and in toxic levels can induce lesions in cardiac and skeletal muscle. Roxarsone can be used in addition to Monensin, increasing its efficacy. Peripheral neuropathy has been shown in chickens that were fed roxarsone supplemented diets and subjected to stress. This paper shows the effects of Monensin in association with Roxarsone in broiler chicken to develop an experimental model that concerns gross and microscopic study of lesions in peripheral nerves and cardiac and skeletal muscle. Broiler ckickens are select due to fast growing and developing. 360 chickens were fed either Monensin and Roxarsona for a period of thirty five days with two regimens (Monensin 300 ppm /Roxarsone 80 ppm ; Monensin 300 ppm /Roxarsone 0 ppm; Monensin 150 ppm /Roxarsone 80 ppm; Monensin 150 ppm /Roxarsone 0 ppm; Monensin 0 ppm /Roxarsone 80ppm and Monensin 0 ppm /Roxarsone 0ppm). After this period, birds were euthanatized and muscles and nerves were removed to submit to microscopic examination. The histopathologic findings showed necrosis and myodegeneration in cardiac and skeletal muscle, and macrophages between the fibers. Microscopic lesions were observed in peripheral nerves and cardiac and skeletal muscles consisting in degeneration of and infiltration of inflammatory cells. The use of the association of these drugs in broilers showed to be adequate as a model for nerve and muscle lesions.109
ABSTRACT Chronic hypertrophic pododermatitis cases in six horses of different breeds, aging 14 mo... more ABSTRACT Chronic hypertrophic pododermatitis cases in six horses of different breeds, aging 14 months to 19 years are described. The lesion begun with a infiltrative tissue in the frog and sole regions of the hoof, characterized by a fast and disorganized growth, with a papillary aspect, white colored in the roots and dark on the extremity, with a necrotic secretion and an extremely fetid odor. Microscopically, an exuberant epidermic proliferative tissue was observed, intermingled with little connective tissue. The horses were divided into two treatment groups. In the first group, including three young mares and a foal, showing lesions in only one limb, a surgical resection of the invasive mass was performed, followed by cauterization of the remaining edges and subsequent daily local application of antiseptic substances. In three of these horses, recurrence of the initial lesion occurred, with fast growth of hyperplasic tissue, affecting almost all the frog and half of the sole. Two horses developed contraction deformities of the hoof. In the second group, one male and one female, each with lesions in two limbs, after surgical debridement of the tissue, the animals received daily applications of picric acid 5%, associated to local use of oxitetracyclin. Although one of these cases required a second surgical intervention for removal of the mass, the horses showed after a period of two to three months total absence of the infiltrative tissue. The use of local picric acid 5% and oxitetracyclin associated to previous surgical debridement showed to be more efficient than the use of antiseptic substances in the treatment of chronic hypertrophic pododermatitis.
The number of cases of Bovine Spongiform Encephalopathy (BSE) in the United Kingdom (UK) and Euro... more The number of cases of Bovine Spongiform Encephalopathy (BSE) in the United Kingdom (UK) and Europe has decreased during the last years. The disease is designated as Classical BSE (C-BSE) once the prion protein involved is very constant and has genetic identity. On the other hand, atypical cases have occurred in several countries, apparently without any relationship with contaminated feeding. Atypical cases, H or L-BSE can have involvement with the etiology of the known C-BSE, first diagnosed in the UK. With the control of C-BSE based on rigid control of feeding, the occurrence of atypical BSE may predominate in the future. In this hypothesis atypical BSE, probably a spontaneous encephalopathy of cattle, will be considered as Sporadic BSE in contrast with the C-BSE that could be nominated UK-BSE.
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Papers by Antonio Alessi