Nepal is an endemic country for dengue infection with rolling of every three year’s clear cyclic ... more Nepal is an endemic country for dengue infection with rolling of every three year’s clear cyclic outbreaks with exponential growth since 2019 outbreak and the virus gearing towards the non-foci temperate hill regions. However, the information regarding circulating serotype and genotype is not frequent. This research discusses on the clinical features, diagnosis, epidemiology, circulating serotype and genotype among 61 dengue suspected cases from different hospitals of Nepal during the window period 2017–2018 between the two outbreaks 2016 and the then expected 2019 outbreak along with the challenges of DENV infection in Nepal. E-gene sequences from PCR positive samples were subjected to phylogenetic analysis under time to most recent common ancestor tree using Markov Chain Monte Carlo (MCMC) and BEAST v2.5.1. Both evolution and genotypes were determined based on the phylogenetic tree. Serotyping by Real-time PCR and Nested PCR showed the co-circulation of all the 3 serotypes of deng...
Good health starts with good food. Humans expect agriculture to supply good food with sufficient ... more Good health starts with good food. Humans expect agriculture to supply good food with sufficient nutrients, economically and culturally valued foods, fibers and other products. But the excessive application of synthetic pesticides has exerted an adverse effect on bio-flora, fauna and natural enemies. Even a largest part of yield has been lost due to various stresses, like biotic and abiotic stresses to the plant. On this account, plant growth promoting rhizobacteria (PGPR), an eco-friendly biopesticides is boon for the biocontrol of different plant pathogens. Moreover, PGPR strains can enhance the plant growth through the production of various plant growth promoting substances. These are generally a group of microorganism that is found either in the plane of the rhizosphere or above roots impacting some positive benefits to plants. PGPR are associated with plant roots and augment plant productivity and immunity; however, recent work by several groups shows that PGPR also elicit so-c...
Antibacterial properties of ethanolic extracts of 8 medicinal plants used in Nepal to treat gastr... more Antibacterial properties of ethanolic extracts of 8 medicinal plants used in Nepal to treat gastrointestinal disorders were tested against ten different species of enteropathogenic bacteria: Escherichia coli, Klebsiella spp, Citrobacter spp, Enterobacter spp, Salmonella typhi, Salmonella paratyphi, Shigella spp, Proteus vulgaris, Proteus mirabilis and Pseudomonas spp. Among the selected medicinal plants: Punica granatum, Woodfordia fruticosa, Psidium guajava and Syzygium cumini were found effective against all enteric bacteria whereas Mimosa pudica, Acorus calamus, Aegle marmelos and Anethum sowa were found ineffective against all. The minimum bactericidal concentration (MBC) of these plant extracts found against Salmonella typhi, Salmonella paratyphi, Proteus mirabilis and Proteus vulgaris were lower (0.39-25mg/ml) so are more susceptible whereas the plants showed lethal effect against Pseudomonas spp, Citrobacter spp, Enterobacter spp, E. coli, Shigella spp. and Klebsiella spp. at...
Nepal is an endemic country for dengue infection with rolling of every three year’s clear cyclic ... more Nepal is an endemic country for dengue infection with rolling of every three year’s clear cyclic outbreaks with exponential growth since 2019 outbreak and the virus gearing towards the non-foci temperate hill regions. However, the information regarding circulating serotype and genotype is not frequent. This research discusses on the clinical features, diagnosis, epidemiology, circulating serotype and genotype among 61 dengue suspected cases from different hospitals of Nepal during the window period 2017–2018 between the two outbreaks 2016 and the then expected 2019 outbreak along with the challenges of DENV infection in Nepal. E-gene sequences from PCR positive samples were subjected to phylogenetic analysis under time to most recent common ancestor tree using Markov Chain Monte Carlo (MCMC) and BEAST v2.5.1. Both evolution and genotypes were determined based on the phylogenetic tree. Serotyping by Real-time PCR and Nested PCR showed the co-circulation of all the 3 serotypes of deng...
Good health starts with good food. Humans expect agriculture to supply good food with sufficient ... more Good health starts with good food. Humans expect agriculture to supply good food with sufficient nutrients, economically and culturally valued foods, fibers and other products. But the excessive application of synthetic pesticides has exerted an adverse effect on bio-flora, fauna and natural enemies. Even a largest part of yield has been lost due to various stresses, like biotic and abiotic stresses to the plant. On this account, plant growth promoting rhizobacteria (PGPR), an eco-friendly biopesticides is boon for the biocontrol of different plant pathogens. Moreover, PGPR strains can enhance the plant growth through the production of various plant growth promoting substances. These are generally a group of microorganism that is found either in the plane of the rhizosphere or above roots impacting some positive benefits to plants. PGPR are associated with plant roots and augment plant productivity and immunity; however, recent work by several groups shows that PGPR also elicit so-c...
Antibacterial properties of ethanolic extracts of 8 medicinal plants used in Nepal to treat gastr... more Antibacterial properties of ethanolic extracts of 8 medicinal plants used in Nepal to treat gastrointestinal disorders were tested against ten different species of enteropathogenic bacteria: Escherichia coli, Klebsiella spp, Citrobacter spp, Enterobacter spp, Salmonella typhi, Salmonella paratyphi, Shigella spp, Proteus vulgaris, Proteus mirabilis and Pseudomonas spp. Among the selected medicinal plants: Punica granatum, Woodfordia fruticosa, Psidium guajava and Syzygium cumini were found effective against all enteric bacteria whereas Mimosa pudica, Acorus calamus, Aegle marmelos and Anethum sowa were found ineffective against all. The minimum bactericidal concentration (MBC) of these plant extracts found against Salmonella typhi, Salmonella paratyphi, Proteus mirabilis and Proteus vulgaris were lower (0.39-25mg/ml) so are more susceptible whereas the plants showed lethal effect against Pseudomonas spp, Citrobacter spp, Enterobacter spp, E. coli, Shigella spp. and Klebsiella spp. at...
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Papers by Anup Muni Bajracharya