Coagulase-positive staphylococcus (CoPS), including methicillin-resistant Staphylococcus aureus (... more Coagulase-positive staphylococcus (CoPS), including methicillin-resistant Staphylococcus aureus (MRSA), poses a global threat. The increasing prevalence of MRSA in Saudi Arabia emphasizes the need for effective management. This study explores the prevalence of virulence-associated genes and antibiotic resistance patterns in CoPS. Nasal swabs from 200 individuals were collected, and standard protocols were used for the isolation, identification, and characterization of CoPS and coagulase-negative staphylococci (CoNS). Additionally, antimicrobial susceptibility testing and PCR were conducted. Bacterial growth was observed in 58.5% of participants, with 12% positive for CoPS and 30% positive for CoNS. Hospital personnel carriers showed a significantly higher proportion of CoNS compared with non-hospital personnel carriers. Non-hospital personnel CoPS strains displayed higher sensitivity to oxacillin than hospital personnel strains. Cefoxitin exhibited the highest sensitivity among β-la...
HAL (Le Centre pour la Communication Scientifique Directe), 2012
Essential oils of the air-dried aerial parts of Achillea fragrantissima were analyzed by GC-MS ch... more Essential oils of the air-dried aerial parts of Achillea fragrantissima were analyzed by GC-MS chromatography. The results show that 48 components were identified in the oils, and main compounds of oils were 4-terpineol (15.65%), Linalool (11%), carvone (9.42%), β-phellandrene (6.2%), γ-terpinene (5.6%), β-pinene (4.55%), verbenone (4.42%), cedrol (3.0%) and ρ-cymene (2.95%). The antimicrobial activity of various extracts from A. fragrantissima was evaluated against 5 fungi and 5 bacteria. Water-soluble extract method I and II from aerial dried powder were inactive against all tested fungi and bacteria. The other extracts were active against the five used fungi except ethanol extract was inactive against all tested fungi. The crude saponin from aerial dried powder was inactive against all tested bacteria. Proteus mirabilis was the only bacterium that was inhibited by the most of the extracts from A. fragrantissima. The only one extract of fresh flowers soaked in ethanol showed activity against all tested bacteria.
Background The coagulase-positive staphylococcus (CoPS) has become progressively prevailing in bo... more Background The coagulase-positive staphylococcus (CoPS) has become progressively prevailing in both community and hospital. The variation between community and hospital acquired methicillin resistant coagulase positive staphylococcus has been used to monitor decisions about realistic therapy. Methods Participants presenting with culture proven coagulase positive staphylococcus from the nose were enclosed during this study. Antibiotic susceptibility test was verified by exploiting the E-test and disk diffusion methods. Eleven toxin genes had been analyzed using a polymerase chain reaction. Results The oxacillin-resistant E-test showed extremely vital resistance in hospital-isolates (34.5%) than in community-isolates (19.2%) (p-value = 0.009). The most effective antibiotic against community acquired CoPS is a quinupristin followed by vancomycin and rifampin then clindamycin. Whereas, clindamycin, sulfamethoxazole/trimethoprim, rifampin, and quinupristin seem to be more effective again...
The objective of this work was to study the chemical, physical and microbiological properties of ... more The objective of this work was to study the chemical, physical and microbiological properties of Dumat Lake in Al-Jouf region, Saudi Arabia. Limnological parameters were evaluated in February 2009 from different lake sites and from northern reservoir (Main source of water to the lake). The temperature and pH values of the lake sites and northern reservoir were measured and ranged between 20.9-22.1°C and 18.8°C and 8.25-8.7 and 7.25, respectively. With respect to temperature and pH-values, all nitrogen forms were all below the standard. The measured hardness values (8390-2105 mg as CaCO LG) indicated that the lake sites water was 3 1 described as very hard but (166 mg as CaCO LG) indicated that the northern reservoir was very soft. The 3 1 surface water quality of Dumat Lake could be classified as class 1. The data obtained from lake sites and northern reservoir could be used as a baseline and reference points when assessing further changes caused by nature or man in the lake. INTROD...
A novel neutral highly thermostable protease was detected in the culture medium of thermophilic B... more A novel neutral highly thermostable protease was detected in the culture medium of thermophilic Bacillus strain HUTBS62 isolated from hot-spring located near to the Dead Sea, Jordan. The enzyme was purified by precipitation with 55-60% ammonium sulfate, gel filtration on Sephadex G-100 and DEAE ion exchange chromatography. The enzyme was purified 53-fold with 2% yield. The optimum pH and temperature for catalytic activity of protease was pH 6.8 and 80oC, respectively, and 31% activity of protease remained even after heat treatment at 100oC for 60 min. The relative activity of the enzyme was highly stable (90%) at 50oC for 2 h. The half-life of the enzyme at 90oC, 80oC and 70oC was estimated to be 3, 4 and 6 h, respectively. The activation energy of denaturation of purified enzyme was 21.7 kJmol -1 . Iron, sodium, calcium, and manganese increased protease activity. On the other hand, magnesium, cobalt and zinc variably decreased the residual activity. But cadmium and copper drastical...
ABSTRACT All tested Bacillus species in this study had the ability to produce extracellular amyla... more ABSTRACT All tested Bacillus species in this study had the ability to produce extracellular amylase when grown at pH 5-10 and at incubation temperature between 43-73°C. Bacillus laterosporus and Bacillus schlegelii were able to produce extracellular amylase at incubation temperature of 28°C. Bacillus sphaericus was the only species that produced extracellular amylase at medium pH of 4. Bacillus circulans showed extracellular amylase activity even in the presence of EDTA in its culture media. The presence of NaCl in the culture media variably promoted extracellular amylase abundance with Bacillus schlegelii was able to secrete amylase even in presence of 5% NaCl. The presence of fructose, maltose, lactose and glucose diminished extracellular amylase in all studied species. Presence of detergents Tween-20 or Tween-80 in the culture medium variably affected secretion of amylase.
International Journal of Environmental Research and Public Health, 2021
Transmission of respiratory viruses is a complex process involving emission, deposition in the ai... more Transmission of respiratory viruses is a complex process involving emission, deposition in the airways, and infection. Inhalation is often the most relevant transmission mode in indoor environments. For severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the risk of inhalation transmission is not yet fully understood. Here, we used an indoor aerosol model combined with a regional inhaled deposited dose model to examine the indoor transport of aerosols from an infected person with novel coronavirus disease (COVID-19) to a susceptible person and assess the potential inhaled dose rate of particles. Two scenarios with different ventilation rates were compared, as well as adult female versus male recipients. Assuming a source strength of 10 viruses/s, in a tightly closed room with poor ventilation (0.5 h−1), the respiratory tract deposited dose rate was 140–350 and 100–260 inhaled viruses/hour for males and females; respectively. With ventilation at 3 h−1 the dose rate was only...
The objective of this work was to study the chemical, physical and microbiological properties of ... more The objective of this work was to study the chemical, physical and microbiological properties of Dumat Lake in Al-Jouf region, Saudi Arabia. Limnological parameters were evaluated in February 2009 from different lake sites and from northern reservoir (Main source of water to the lake). The temperature and pH values of the lake sites and northern reservoir were measured and ranged between 20.9-22.1°C and 18.8°C and 8.25-8.7 and 7.25, respectively. With respect to temperature and pH-values, all nitrogen forms were all below the standard. The measured hardness values (8390-2105 mg as CaCO LG) indicated that the lake sites water was 3 1 described as very hard but (166 mg as CaCO LG) indicated that the northern reservoir was very soft. The 3 1 surface water quality of Dumat Lake could be classified as class 1. The data obtained from lake sites and northern reservoir could be used as a baseline and reference points when assessing further changes caused by nature or man in the lake. INTROD...
The thermophilic proteases were purified from two different Bacillus strains HUTBS62 and HUTBS71.... more The thermophilic proteases were purified from two different Bacillus strains HUTBS62 and HUTBS71. The two strains showed variation in ability to grow at different pH-values and temperatures, pH 5-11 and 28-73 o C (HUTBS71) and pH 5-7 and 37-63 o C (HUTBS62), respectively. The purified enzyme from the two different strains also showed variation in purification folds and %yields in different steps of purification methods. Ammonium sulfate fractionation was achieved at 75-80% for HUTBS71 and 55-60% concentrations for HUTBS62. The purification fold and yield were 10-fold and 67% for strain HUTBS71, and 6.5-fold and 61% for strain HUTBS62, respectively. Sephadex G-100 purification step achieved 40-fold purification and 16.7% yield from strain HUTBS71, and 32-fold purification and 12% yield of protease from strain HUTBS62. DEAE ion exchange chromatography step achieved 60-fold purification and 1.7% yield for strain HUTBS71, and 53-fold purification and 2% yield for strain HUTBS62. The mol...
The growth, swarming and production of halo-zone by six different strains of P. mirabilis were ex... more The growth, swarming and production of halo-zone by six different strains of P. mirabilis were examined on different selective media. Inability of strains to grow on different selective medium commonly showed at 25°C. The swarming did not observe on Baired-Parker stable base agar and cetrimide agar medium at both used temperatures (25 and 37°C); whereas no swarming observed on desoxycholate citrate lactose agar medium at 25°C and on violet red bile agar medium at 37°C. Both strains (PM4 and PM5) were able to produce hydrogen sulfide (H2S) at both used temperatures; in contrast, the other two strains (PM2 and PM3) were H2S negative. But PM1 and PM6 were H2S positive at 37°C and negative at 25°C. Several compounds inhibit the motility of swarmer cells by disturbing the structural integrity, the activity of flagella or by blocking their ability to move. Those compounds may also inhibit swarming but without affecting growth.
Essential oils of the air-dried aerial parts of Achillea fragrantissima were analyzed by GC-MS ch... more Essential oils of the air-dried aerial parts of Achillea fragrantissima were analyzed by GC-MS chromatography. The results show that 48 components were identified in the oils, and main compounds of oils were 4-terpineol (15.65%), Linalool (11%), carvone (9.42%), β-phellandrene (6.2%), γ-terpinene (5.6%), β-pinene (4.55%), verbenone (4.42%), cedrol (3.0%) and ρ-cymene (2.95%). The antimicrobial activity of various extracts from A. fragrantissima was evaluated against 5 fungi and 5 bacteria. Water-soluble extract method I and II from aerial dried powder were inactive against all tested fungi and bacteria. The other extracts were active against the five used fungi except ethanol extract was inactive against all tested fungi. The crude saponin from aerial dried powder was inactive against all tested bacteria. Proteus mirabilis was the only bacterium that was inhibited by the most of the extracts from A. fragrantissima. The only one extract of fresh flowers soaked in ethanol showed activ...
Coagulase-positive staphylococcus (CoPS), including methicillin-resistant Staphylococcus aureus (... more Coagulase-positive staphylococcus (CoPS), including methicillin-resistant Staphylococcus aureus (MRSA), poses a global threat. The increasing prevalence of MRSA in Saudi Arabia emphasizes the need for effective management. This study explores the prevalence of virulence-associated genes and antibiotic resistance patterns in CoPS. Nasal swabs from 200 individuals were collected, and standard protocols were used for the isolation, identification, and characterization of CoPS and coagulase-negative staphylococci (CoNS). Additionally, antimicrobial susceptibility testing and PCR were conducted. Bacterial growth was observed in 58.5% of participants, with 12% positive for CoPS and 30% positive for CoNS. Hospital personnel carriers showed a significantly higher proportion of CoNS compared with non-hospital personnel carriers. Non-hospital personnel CoPS strains displayed higher sensitivity to oxacillin than hospital personnel strains. Cefoxitin exhibited the highest sensitivity among β-la...
HAL (Le Centre pour la Communication Scientifique Directe), 2012
Essential oils of the air-dried aerial parts of Achillea fragrantissima were analyzed by GC-MS ch... more Essential oils of the air-dried aerial parts of Achillea fragrantissima were analyzed by GC-MS chromatography. The results show that 48 components were identified in the oils, and main compounds of oils were 4-terpineol (15.65%), Linalool (11%), carvone (9.42%), β-phellandrene (6.2%), γ-terpinene (5.6%), β-pinene (4.55%), verbenone (4.42%), cedrol (3.0%) and ρ-cymene (2.95%). The antimicrobial activity of various extracts from A. fragrantissima was evaluated against 5 fungi and 5 bacteria. Water-soluble extract method I and II from aerial dried powder were inactive against all tested fungi and bacteria. The other extracts were active against the five used fungi except ethanol extract was inactive against all tested fungi. The crude saponin from aerial dried powder was inactive against all tested bacteria. Proteus mirabilis was the only bacterium that was inhibited by the most of the extracts from A. fragrantissima. The only one extract of fresh flowers soaked in ethanol showed activity against all tested bacteria.
Background The coagulase-positive staphylococcus (CoPS) has become progressively prevailing in bo... more Background The coagulase-positive staphylococcus (CoPS) has become progressively prevailing in both community and hospital. The variation between community and hospital acquired methicillin resistant coagulase positive staphylococcus has been used to monitor decisions about realistic therapy. Methods Participants presenting with culture proven coagulase positive staphylococcus from the nose were enclosed during this study. Antibiotic susceptibility test was verified by exploiting the E-test and disk diffusion methods. Eleven toxin genes had been analyzed using a polymerase chain reaction. Results The oxacillin-resistant E-test showed extremely vital resistance in hospital-isolates (34.5%) than in community-isolates (19.2%) (p-value = 0.009). The most effective antibiotic against community acquired CoPS is a quinupristin followed by vancomycin and rifampin then clindamycin. Whereas, clindamycin, sulfamethoxazole/trimethoprim, rifampin, and quinupristin seem to be more effective again...
The objective of this work was to study the chemical, physical and microbiological properties of ... more The objective of this work was to study the chemical, physical and microbiological properties of Dumat Lake in Al-Jouf region, Saudi Arabia. Limnological parameters were evaluated in February 2009 from different lake sites and from northern reservoir (Main source of water to the lake). The temperature and pH values of the lake sites and northern reservoir were measured and ranged between 20.9-22.1°C and 18.8°C and 8.25-8.7 and 7.25, respectively. With respect to temperature and pH-values, all nitrogen forms were all below the standard. The measured hardness values (8390-2105 mg as CaCO LG) indicated that the lake sites water was 3 1 described as very hard but (166 mg as CaCO LG) indicated that the northern reservoir was very soft. The 3 1 surface water quality of Dumat Lake could be classified as class 1. The data obtained from lake sites and northern reservoir could be used as a baseline and reference points when assessing further changes caused by nature or man in the lake. INTROD...
A novel neutral highly thermostable protease was detected in the culture medium of thermophilic B... more A novel neutral highly thermostable protease was detected in the culture medium of thermophilic Bacillus strain HUTBS62 isolated from hot-spring located near to the Dead Sea, Jordan. The enzyme was purified by precipitation with 55-60% ammonium sulfate, gel filtration on Sephadex G-100 and DEAE ion exchange chromatography. The enzyme was purified 53-fold with 2% yield. The optimum pH and temperature for catalytic activity of protease was pH 6.8 and 80oC, respectively, and 31% activity of protease remained even after heat treatment at 100oC for 60 min. The relative activity of the enzyme was highly stable (90%) at 50oC for 2 h. The half-life of the enzyme at 90oC, 80oC and 70oC was estimated to be 3, 4 and 6 h, respectively. The activation energy of denaturation of purified enzyme was 21.7 kJmol -1 . Iron, sodium, calcium, and manganese increased protease activity. On the other hand, magnesium, cobalt and zinc variably decreased the residual activity. But cadmium and copper drastical...
ABSTRACT All tested Bacillus species in this study had the ability to produce extracellular amyla... more ABSTRACT All tested Bacillus species in this study had the ability to produce extracellular amylase when grown at pH 5-10 and at incubation temperature between 43-73°C. Bacillus laterosporus and Bacillus schlegelii were able to produce extracellular amylase at incubation temperature of 28°C. Bacillus sphaericus was the only species that produced extracellular amylase at medium pH of 4. Bacillus circulans showed extracellular amylase activity even in the presence of EDTA in its culture media. The presence of NaCl in the culture media variably promoted extracellular amylase abundance with Bacillus schlegelii was able to secrete amylase even in presence of 5% NaCl. The presence of fructose, maltose, lactose and glucose diminished extracellular amylase in all studied species. Presence of detergents Tween-20 or Tween-80 in the culture medium variably affected secretion of amylase.
International Journal of Environmental Research and Public Health, 2021
Transmission of respiratory viruses is a complex process involving emission, deposition in the ai... more Transmission of respiratory viruses is a complex process involving emission, deposition in the airways, and infection. Inhalation is often the most relevant transmission mode in indoor environments. For severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the risk of inhalation transmission is not yet fully understood. Here, we used an indoor aerosol model combined with a regional inhaled deposited dose model to examine the indoor transport of aerosols from an infected person with novel coronavirus disease (COVID-19) to a susceptible person and assess the potential inhaled dose rate of particles. Two scenarios with different ventilation rates were compared, as well as adult female versus male recipients. Assuming a source strength of 10 viruses/s, in a tightly closed room with poor ventilation (0.5 h−1), the respiratory tract deposited dose rate was 140–350 and 100–260 inhaled viruses/hour for males and females; respectively. With ventilation at 3 h−1 the dose rate was only...
The objective of this work was to study the chemical, physical and microbiological properties of ... more The objective of this work was to study the chemical, physical and microbiological properties of Dumat Lake in Al-Jouf region, Saudi Arabia. Limnological parameters were evaluated in February 2009 from different lake sites and from northern reservoir (Main source of water to the lake). The temperature and pH values of the lake sites and northern reservoir were measured and ranged between 20.9-22.1°C and 18.8°C and 8.25-8.7 and 7.25, respectively. With respect to temperature and pH-values, all nitrogen forms were all below the standard. The measured hardness values (8390-2105 mg as CaCO LG) indicated that the lake sites water was 3 1 described as very hard but (166 mg as CaCO LG) indicated that the northern reservoir was very soft. The 3 1 surface water quality of Dumat Lake could be classified as class 1. The data obtained from lake sites and northern reservoir could be used as a baseline and reference points when assessing further changes caused by nature or man in the lake. INTROD...
The thermophilic proteases were purified from two different Bacillus strains HUTBS62 and HUTBS71.... more The thermophilic proteases were purified from two different Bacillus strains HUTBS62 and HUTBS71. The two strains showed variation in ability to grow at different pH-values and temperatures, pH 5-11 and 28-73 o C (HUTBS71) and pH 5-7 and 37-63 o C (HUTBS62), respectively. The purified enzyme from the two different strains also showed variation in purification folds and %yields in different steps of purification methods. Ammonium sulfate fractionation was achieved at 75-80% for HUTBS71 and 55-60% concentrations for HUTBS62. The purification fold and yield were 10-fold and 67% for strain HUTBS71, and 6.5-fold and 61% for strain HUTBS62, respectively. Sephadex G-100 purification step achieved 40-fold purification and 16.7% yield from strain HUTBS71, and 32-fold purification and 12% yield of protease from strain HUTBS62. DEAE ion exchange chromatography step achieved 60-fold purification and 1.7% yield for strain HUTBS71, and 53-fold purification and 2% yield for strain HUTBS62. The mol...
The growth, swarming and production of halo-zone by six different strains of P. mirabilis were ex... more The growth, swarming and production of halo-zone by six different strains of P. mirabilis were examined on different selective media. Inability of strains to grow on different selective medium commonly showed at 25°C. The swarming did not observe on Baired-Parker stable base agar and cetrimide agar medium at both used temperatures (25 and 37°C); whereas no swarming observed on desoxycholate citrate lactose agar medium at 25°C and on violet red bile agar medium at 37°C. Both strains (PM4 and PM5) were able to produce hydrogen sulfide (H2S) at both used temperatures; in contrast, the other two strains (PM2 and PM3) were H2S negative. But PM1 and PM6 were H2S positive at 37°C and negative at 25°C. Several compounds inhibit the motility of swarmer cells by disturbing the structural integrity, the activity of flagella or by blocking their ability to move. Those compounds may also inhibit swarming but without affecting growth.
Essential oils of the air-dried aerial parts of Achillea fragrantissima were analyzed by GC-MS ch... more Essential oils of the air-dried aerial parts of Achillea fragrantissima were analyzed by GC-MS chromatography. The results show that 48 components were identified in the oils, and main compounds of oils were 4-terpineol (15.65%), Linalool (11%), carvone (9.42%), β-phellandrene (6.2%), γ-terpinene (5.6%), β-pinene (4.55%), verbenone (4.42%), cedrol (3.0%) and ρ-cymene (2.95%). The antimicrobial activity of various extracts from A. fragrantissima was evaluated against 5 fungi and 5 bacteria. Water-soluble extract method I and II from aerial dried powder were inactive against all tested fungi and bacteria. The other extracts were active against the five used fungi except ethanol extract was inactive against all tested fungi. The crude saponin from aerial dried powder was inactive against all tested bacteria. Proteus mirabilis was the only bacterium that was inhibited by the most of the extracts from A. fragrantissima. The only one extract of fresh flowers soaked in ethanol showed activ...
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