Objectives Visceral Leishmaniasis (VL) can be symptomatic and asymptomatic infection. In this stu... more Objectives Visceral Leishmaniasis (VL) can be symptomatic and asymptomatic infection. In this study we aimed at investigating the prevalence of asymptomatic infections and to study risk factors of VL in non-endemic regions in Gedaref state, Sudan. A descriptive cross-sectional study conducted during 2014. Blood samples were collected to serological and molecular analysis. Sticky traps, knockdown spray and CDC miniature light traps were used for the collection of sandflies. Results Ninety-Five participants were included; 52 from Abukishma, 15 Algadamblia Tirfa, 25 Abualnaja and 3 were from Algadamblia Aljabal. Most of the study participants were belonging to the Belala tribe (74.3%). The most frequent reported age was above 40-years old (9.5%). Females were (61.1%) and males were (38.9%). B. aeygptica was the most planted tree in/around the houses (46.3%). 73 (76.8%) of the participants bred more than two types of animals in the house. DAT test revealed 5 positive participants (5.2%)...
The deadly malaria parasite, Plasmodium falciparum, contains a unique subcellular organelle terme... more The deadly malaria parasite, Plasmodium falciparum, contains a unique subcellular organelle termed the apicoplast, which is a clinically-proven antimalarial drug target. The apicoplast is a plastid with essential metabolic functions that evolved via secondary endosymbiosis. As an ancient endosymbiont, the apicoplast retained its own genome and it must be inherited by daughter cells during cell division. During the asexual replication of P. falciparum inside human red blood cells, both the parasite, and the apicoplast inside it, undergo massive morphological changes, including DNA replication and division. The apicoplast is an integral part of the cell and thus its development is tightly synchronized with the cell cycle. At the same time, certain aspects of its dynamics are independent of nuclear division, representing a degree of autonomy in organelle biogenesis. Here, we review the different aspects of organelle dynamics during P. falciparum intraerythrocytic replication, summarize...
this hospital based cross-sectional study conducted among Sudanese women with ovarian cancer <... more this hospital based cross-sectional study conducted among Sudanese women with ovarian cancer <br><br>
this hospital based cross-sectional study conducted among Sudanese women with ovarian cancer <... more this hospital based cross-sectional study conducted among Sudanese women with ovarian cancer <br><br>
this hospital based cross-sectional study conducted among Sudanese women with ovarian cancer <... more this hospital based cross-sectional study conducted among Sudanese women with ovarian cancer <br><br>
this hospital based cross-sectional study conducted among Sudanese women with ovarian cancer <... more this hospital based cross-sectional study conducted among Sudanese women with ovarian cancer <br><br>
Abundance of aquatic stages of Simulium black fly collected from different sites in Galabat sub-f... more Abundance of aquatic stages of Simulium black fly collected from different sites in Galabat sub-focus, during 2009, 2013 and 2015. (DOCX 26 kb)
Additional file 3. Association between VL infection and demographic, household/environmental, beh... more Additional file 3. Association between VL infection and demographic, household/environmental, behavioral factors.
Abstract: Background: Sudan is a Sub-Saharan African country with a high prevalence rate of Tuber... more Abstract: Background: Sudan is a Sub-Saharan African country with a high prevalence rate of Tuberculosis. Natural Resistant Associated Macrophage Protein 1 (NRAMP1) plays a potential role in the development of immunity against TB, and it has a critical role in disease resistance. The aim of the study was to evaluate the association of NRAMP1 polymorphism at intron4 (INT4) region with susceptibility to TB infection. Methods: Demographic, clinical and microbiological data were collected from 150 participants and investigated using designed questionnaire. The genotyping of NRAMP1-INT4 polymorphism was performed in 60 TB-infected patients and 50 healthy control using Polymerase chain reaction and restriction fragment-length polymorphism method (PCR-RFLP). Results: Among cases (60%) were males, only (3.3%) were vaccinated. The most reported risk factors were tobacco smoking (17%), diabetes (10%), alcohol consumption (2%) and corticosteroid therapy intakes (1%). Pulmonary TB was detected ...
Background Current malaria control and elimination strategies rely mainly on efficacious antimala... more Background Current malaria control and elimination strategies rely mainly on efficacious antimalarial drugs. However, drug resistance is a major threat facing malaria control programs. Determination of drug resistance molecular markers is useful in the monitoring and surveillance of malaria drug efficacy. This study aimed to determine the mutations and haplotypes frequencies of different genes linked with antimalarial drug resistance in certain areas in Sudan. Methods A total of 226 dried blood spots (DBS) of microscopically diagnosed P. falciparum isolates were collected from Khartoum and three other areas in Sudan during 2015–2017. Plasmodium falciparum confirmation and multiplicity of infection was assessed using the Sanger’s 101 SNPs-barcode and speciation was confirmed using regions of the parasite mitochondria. Molecular genotyping of drug resistance genes (Pfcrt, Pfmdr1, Pfdhfr, Pfdhps, exonuclease, Pfk13, parasite genetic background (PGB) (Pfarps10, ferredoxin, Pfcrt, Pfmdr2...
Objectives Visceral Leishmaniasis (VL) can be symptomatic and asymptomatic infection. In this stu... more Objectives Visceral Leishmaniasis (VL) can be symptomatic and asymptomatic infection. In this study we aimed at investigating the prevalence of asymptomatic infections and to study risk factors of VL in non-endemic regions in Gedaref state, Sudan. A descriptive cross-sectional study conducted during 2014. Blood samples were collected to serological and molecular analysis. Sticky traps, knockdown spray and CDC miniature light traps were used for the collection of sandflies. Results Ninety-Five participants were included; 52 from Abukishma, 15 Algadamblia Tirfa, 25 Abualnaja and 3 were from Algadamblia Aljabal. Most of the study participants were belonging to the Belala tribe (74.3%). The most frequent reported age was above 40-years old (9.5%). Females were (61.1%) and males were (38.9%). B. aeygptica was the most planted tree in/around the houses (46.3%). 73 (76.8%) of the participants bred more than two types of animals in the house. DAT test revealed 5 positive participants (5.2%)...
The deadly malaria parasite, Plasmodium falciparum, contains a unique subcellular organelle terme... more The deadly malaria parasite, Plasmodium falciparum, contains a unique subcellular organelle termed the apicoplast, which is a clinically-proven antimalarial drug target. The apicoplast is a plastid with essential metabolic functions that evolved via secondary endosymbiosis. As an ancient endosymbiont, the apicoplast retained its own genome and it must be inherited by daughter cells during cell division. During the asexual replication of P. falciparum inside human red blood cells, both the parasite, and the apicoplast inside it, undergo massive morphological changes, including DNA replication and division. The apicoplast is an integral part of the cell and thus its development is tightly synchronized with the cell cycle. At the same time, certain aspects of its dynamics are independent of nuclear division, representing a degree of autonomy in organelle biogenesis. Here, we review the different aspects of organelle dynamics during P. falciparum intraerythrocytic replication, summarize...
this hospital based cross-sectional study conducted among Sudanese women with ovarian cancer <... more this hospital based cross-sectional study conducted among Sudanese women with ovarian cancer <br><br>
this hospital based cross-sectional study conducted among Sudanese women with ovarian cancer <... more this hospital based cross-sectional study conducted among Sudanese women with ovarian cancer <br><br>
this hospital based cross-sectional study conducted among Sudanese women with ovarian cancer <... more this hospital based cross-sectional study conducted among Sudanese women with ovarian cancer <br><br>
this hospital based cross-sectional study conducted among Sudanese women with ovarian cancer <... more this hospital based cross-sectional study conducted among Sudanese women with ovarian cancer <br><br>
Abundance of aquatic stages of Simulium black fly collected from different sites in Galabat sub-f... more Abundance of aquatic stages of Simulium black fly collected from different sites in Galabat sub-focus, during 2009, 2013 and 2015. (DOCX 26 kb)
Additional file 3. Association between VL infection and demographic, household/environmental, beh... more Additional file 3. Association between VL infection and demographic, household/environmental, behavioral factors.
Abstract: Background: Sudan is a Sub-Saharan African country with a high prevalence rate of Tuber... more Abstract: Background: Sudan is a Sub-Saharan African country with a high prevalence rate of Tuberculosis. Natural Resistant Associated Macrophage Protein 1 (NRAMP1) plays a potential role in the development of immunity against TB, and it has a critical role in disease resistance. The aim of the study was to evaluate the association of NRAMP1 polymorphism at intron4 (INT4) region with susceptibility to TB infection. Methods: Demographic, clinical and microbiological data were collected from 150 participants and investigated using designed questionnaire. The genotyping of NRAMP1-INT4 polymorphism was performed in 60 TB-infected patients and 50 healthy control using Polymerase chain reaction and restriction fragment-length polymorphism method (PCR-RFLP). Results: Among cases (60%) were males, only (3.3%) were vaccinated. The most reported risk factors were tobacco smoking (17%), diabetes (10%), alcohol consumption (2%) and corticosteroid therapy intakes (1%). Pulmonary TB was detected ...
Background Current malaria control and elimination strategies rely mainly on efficacious antimala... more Background Current malaria control and elimination strategies rely mainly on efficacious antimalarial drugs. However, drug resistance is a major threat facing malaria control programs. Determination of drug resistance molecular markers is useful in the monitoring and surveillance of malaria drug efficacy. This study aimed to determine the mutations and haplotypes frequencies of different genes linked with antimalarial drug resistance in certain areas in Sudan. Methods A total of 226 dried blood spots (DBS) of microscopically diagnosed P. falciparum isolates were collected from Khartoum and three other areas in Sudan during 2015–2017. Plasmodium falciparum confirmation and multiplicity of infection was assessed using the Sanger’s 101 SNPs-barcode and speciation was confirmed using regions of the parasite mitochondria. Molecular genotyping of drug resistance genes (Pfcrt, Pfmdr1, Pfdhfr, Pfdhps, exonuclease, Pfk13, parasite genetic background (PGB) (Pfarps10, ferredoxin, Pfcrt, Pfmdr2...
Uploads
Papers by Arwa Elaagip