Liquid holding (LH) recovery was matched with three-way differential staining (TWD) to assess the... more Liquid holding (LH) recovery was matched with three-way differential staining (TWD) to assess the reduction of damage induced in DNA following treatment with 4-nitroquinoline-1-oxide (4NQO) in resting (GO) lymphocytes. Human peripheral lymphocytes (HPL) from three donors were used to evaluate lesion persistence and individual repair capacity. Our data are in contrast to those for diepoxybutane (Ponzanelli et al., 1995) and suggest that LH recovery is completely inefficient in removing 4NQO induced lesions, which are only partially repaired after one cell cycle.
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 1991
... Crebelli, R., S. Fuselli, A. Meneguz, G. Aquilina, L. Conti, P. Leopardi, A. Zijno, F. Baris ... more ... Crebelli, R., S. Fuselli, A. Meneguz, G. Aquilina, L. Conti, P. Leopardi, A. Zijno, F. Baris and A. Carare (1988) In vitro and in vivo mutagenicity studies with airborne particulate extracts, Mutation Res., 204, 565-575. De Flora, S., M. Bagnasco, A. Izzotti, F. D'Agostini, M. Pala and F ...
Sister-chromatid exchanges (SCE) in peripheral blood lymphocytes and mutagenicity of urine (Ames ... more Sister-chromatid exchanges (SCE) in peripheral blood lymphocytes and mutagenicity of urine (Ames test) were measured in a group of 21 nurses professionally handling antineoplastic drugs and in a group of 21 unexposed controls. No differences in SCE frequencies and in urinary mutagenic activity between exposed and unexposed groups were detected. A clear positive increase in urinary mutagenicity in the TA98 Salmonella strain was observed with increasing number of cigarettes smoked, whereas no evident influence of smoking on SCE was seen. Age, coffee and alcohol consumption did not show any detectable effect in the two tests.
Optical genotyping of C3435T single nucleotide polymorphisms (SNPs) in unamplified human multidru... more Optical genotyping of C3435T single nucleotide polymorphisms (SNPs) in unamplified human multidrug resistance (MDR1) gene was here performed by a surface plasmon resonance imaging (SPRi) dual-targeting DNA assay, allowing its selective detection down to 0.18 fM of the whole genomic DNA. The result was achieved by the combination of the rational selection of the DNA probe and an optimized sample pretreatment (i.e., ultrasound fragmentation and thermal denaturation). Some assay developments and tunings were reported in a previously published research, but here, for the first time, the biosensor reliability and its analytical performance were directly tested on the unamplified human DNA extracted from lymphocytes. The assay resulted to be able to differentiate among all the possible genotypes of C3435T (homozygote and heterozygote) in the diluted genomic samples using a label-free approach and by bypassing the classical PCR amplification of the target sequences. Moreover, the reusability of the DNA-based chip allowed up to 40 subsequent measuring cycles, opening new horizons in multi-SNP genotyping based on cheap and daily routine clinical monitoring by optical biosensing.
Background: Postmortem studies suggest excessive free radical toxicity in the substantia nigra of... more Background: Postmortem studies suggest excessive free radical toxicity in the substantia nigra of patients with PD. Increased lipid peroxidation and oxidative DNA damage have been reported in the CNS. Markers of oxidative stress have been identified in the blood of patients with PD.Objective: To assess the presence of spontaneous chromosome and primary or oxidative DNA damage in peripheral blood leukocytes of patients with untreated PD.Methods: Patients with de novo PD (20) and control subjects (16), matched for age, sex, and smoking habits, underwent cytogenetic analysis using the human lymphocyte micronucleus assay coupled with the fluorescence in situ hybridization technique and the Comet assay.Results: Compared with controls, patients with PD showed an increase in the incidence of spontaneous micronuclei (p < 0.001); single strand breaks (p < 0.001); and oxidized purine bases (p < 0.05). Fluorescence in situ hybridization analysis showed micronuclei harboring acentric f...
This study examines the response to dexamethasone-doxorubicin-vincristine (DAV) therapy, followed... more This study examines the response to dexamethasone-doxorubicin-vincristine (DAV) therapy, followed by conditioning regimen and autologous stem cells transplantation (ASCT) in patients with multiple myeloma in relation with the presence of polymorphisms in genes involved in drug metabolism (GSTP1) and DNA synthesis (TYMS). GSTP1 G313G genotype (OR=5.49; 95% CI, 1.3-22.5, p=0.02) and TYMS A227A genotype (OR=3.41; 95% CI, 1.3-8.9, p=0.01) resulted significantly associated with a poor response following chemotherapy and the risk increased for the combined genotype (OR=13.54; 95% CI, 2.0-91.3, p=0.01). TYMS T157T genotype was significantly associated with a poor response after ASCT (OR=4.60; 95% CI, 1.2-16.9, p=0.02). Pre-therapeutic individual determination of the GSTP1 and TYMS polymorphisms could help in choosing the most appropriate protocol.
International Journal of Environmental Health Research, 1991
Page 1. International Journal of Environmental Health Research1, 37-53 (1991) Correlation between... more Page 1. International Journal of Environmental Health Research1, 37-53 (1991) Correlation between mutagenicity of airborne particles and air pollution parameters in eleven Italian towns R. BARALE1, F. GIORGELLI2, R. SCARPATO2 ...
Using whole-chromosome painting probes, we have analyzed the frequency of translocations of chrom... more Using whole-chromosome painting probes, we have analyzed the frequency of translocations of chromosomes 1, 3, and 10 in peripheral lymphocytes of 20 Gomel (Belarus) children, including both thyroid tumor affected and healthy individuals. Gomel was one of the most heavily radiocontaminated areas due to fallout from the Chernobyl nuclear power plant disaster. As controls, 14 healthy children from Pisa (Italy) were investigated simultaneously. Translocation rates were significantly higher in the tumor affected (1.71 ± 0.68) and healthy Gomel children (2.69 ± 0.50) than in the Italian controls (0.79 ± 0.24). We also observed, in healthy Gomel children, an approximately three-fold higher frequency of chromosome 10 translocations compared to translocations affecting chromosomes 1 or 3 (P = 0.0096), a difference that was made even larger (about four fold) after correcting for chromosome size (P = 0.0009). This finding suggests a preferential involvement of chromosome 10 in translocations i...
Surname distributions were studied in records of male and female births in Ferrara in the period ... more Surname distributions were studied in records of male and female births in Ferrara in the period 1982-89, and in records of male and female deaths in the same period. Average year of birth and standard deviation was 1985 +/- 2.3 for the birth series, and 1912 +/- 14.4 for the death series. Then the surname distributions, in two independent samples at an average distance of 73 years, were compared. It was observed that random isonymy within series, which depends on the shape of the distribution, stays fairly constant at three generations of distance, indicating near-equilibrium of surname turnover. The migrational contribution is indicated by the significant decrease of random isonymy between series, measured with the method of Lasker. It was also observed that immigrants to Ferrara have a life-span significantly longer than people born in the town.
Liquid holding (LH) recovery was matched with three-way differential staining (TWD) to assess the... more Liquid holding (LH) recovery was matched with three-way differential staining (TWD) to assess the reduction of damage induced in DNA following treatment with 4-nitroquinoline-1-oxide (4NQO) in resting (GO) lymphocytes. Human peripheral lymphocytes (HPL) from three donors were used to evaluate lesion persistence and individual repair capacity. Our data are in contrast to those for diepoxybutane (Ponzanelli et al., 1995) and suggest that LH recovery is completely inefficient in removing 4NQO induced lesions, which are only partially repaired after one cell cycle.
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 1991
... Crebelli, R., S. Fuselli, A. Meneguz, G. Aquilina, L. Conti, P. Leopardi, A. Zijno, F. Baris ... more ... Crebelli, R., S. Fuselli, A. Meneguz, G. Aquilina, L. Conti, P. Leopardi, A. Zijno, F. Baris and A. Carare (1988) In vitro and in vivo mutagenicity studies with airborne particulate extracts, Mutation Res., 204, 565-575. De Flora, S., M. Bagnasco, A. Izzotti, F. D'Agostini, M. Pala and F ...
Sister-chromatid exchanges (SCE) in peripheral blood lymphocytes and mutagenicity of urine (Ames ... more Sister-chromatid exchanges (SCE) in peripheral blood lymphocytes and mutagenicity of urine (Ames test) were measured in a group of 21 nurses professionally handling antineoplastic drugs and in a group of 21 unexposed controls. No differences in SCE frequencies and in urinary mutagenic activity between exposed and unexposed groups were detected. A clear positive increase in urinary mutagenicity in the TA98 Salmonella strain was observed with increasing number of cigarettes smoked, whereas no evident influence of smoking on SCE was seen. Age, coffee and alcohol consumption did not show any detectable effect in the two tests.
Optical genotyping of C3435T single nucleotide polymorphisms (SNPs) in unamplified human multidru... more Optical genotyping of C3435T single nucleotide polymorphisms (SNPs) in unamplified human multidrug resistance (MDR1) gene was here performed by a surface plasmon resonance imaging (SPRi) dual-targeting DNA assay, allowing its selective detection down to 0.18 fM of the whole genomic DNA. The result was achieved by the combination of the rational selection of the DNA probe and an optimized sample pretreatment (i.e., ultrasound fragmentation and thermal denaturation). Some assay developments and tunings were reported in a previously published research, but here, for the first time, the biosensor reliability and its analytical performance were directly tested on the unamplified human DNA extracted from lymphocytes. The assay resulted to be able to differentiate among all the possible genotypes of C3435T (homozygote and heterozygote) in the diluted genomic samples using a label-free approach and by bypassing the classical PCR amplification of the target sequences. Moreover, the reusability of the DNA-based chip allowed up to 40 subsequent measuring cycles, opening new horizons in multi-SNP genotyping based on cheap and daily routine clinical monitoring by optical biosensing.
Background: Postmortem studies suggest excessive free radical toxicity in the substantia nigra of... more Background: Postmortem studies suggest excessive free radical toxicity in the substantia nigra of patients with PD. Increased lipid peroxidation and oxidative DNA damage have been reported in the CNS. Markers of oxidative stress have been identified in the blood of patients with PD.Objective: To assess the presence of spontaneous chromosome and primary or oxidative DNA damage in peripheral blood leukocytes of patients with untreated PD.Methods: Patients with de novo PD (20) and control subjects (16), matched for age, sex, and smoking habits, underwent cytogenetic analysis using the human lymphocyte micronucleus assay coupled with the fluorescence in situ hybridization technique and the Comet assay.Results: Compared with controls, patients with PD showed an increase in the incidence of spontaneous micronuclei (p < 0.001); single strand breaks (p < 0.001); and oxidized purine bases (p < 0.05). Fluorescence in situ hybridization analysis showed micronuclei harboring acentric f...
This study examines the response to dexamethasone-doxorubicin-vincristine (DAV) therapy, followed... more This study examines the response to dexamethasone-doxorubicin-vincristine (DAV) therapy, followed by conditioning regimen and autologous stem cells transplantation (ASCT) in patients with multiple myeloma in relation with the presence of polymorphisms in genes involved in drug metabolism (GSTP1) and DNA synthesis (TYMS). GSTP1 G313G genotype (OR=5.49; 95% CI, 1.3-22.5, p=0.02) and TYMS A227A genotype (OR=3.41; 95% CI, 1.3-8.9, p=0.01) resulted significantly associated with a poor response following chemotherapy and the risk increased for the combined genotype (OR=13.54; 95% CI, 2.0-91.3, p=0.01). TYMS T157T genotype was significantly associated with a poor response after ASCT (OR=4.60; 95% CI, 1.2-16.9, p=0.02). Pre-therapeutic individual determination of the GSTP1 and TYMS polymorphisms could help in choosing the most appropriate protocol.
International Journal of Environmental Health Research, 1991
Page 1. International Journal of Environmental Health Research1, 37-53 (1991) Correlation between... more Page 1. International Journal of Environmental Health Research1, 37-53 (1991) Correlation between mutagenicity of airborne particles and air pollution parameters in eleven Italian towns R. BARALE1, F. GIORGELLI2, R. SCARPATO2 ...
Using whole-chromosome painting probes, we have analyzed the frequency of translocations of chrom... more Using whole-chromosome painting probes, we have analyzed the frequency of translocations of chromosomes 1, 3, and 10 in peripheral lymphocytes of 20 Gomel (Belarus) children, including both thyroid tumor affected and healthy individuals. Gomel was one of the most heavily radiocontaminated areas due to fallout from the Chernobyl nuclear power plant disaster. As controls, 14 healthy children from Pisa (Italy) were investigated simultaneously. Translocation rates were significantly higher in the tumor affected (1.71 ± 0.68) and healthy Gomel children (2.69 ± 0.50) than in the Italian controls (0.79 ± 0.24). We also observed, in healthy Gomel children, an approximately three-fold higher frequency of chromosome 10 translocations compared to translocations affecting chromosomes 1 or 3 (P = 0.0096), a difference that was made even larger (about four fold) after correcting for chromosome size (P = 0.0009). This finding suggests a preferential involvement of chromosome 10 in translocations i...
Surname distributions were studied in records of male and female births in Ferrara in the period ... more Surname distributions were studied in records of male and female births in Ferrara in the period 1982-89, and in records of male and female deaths in the same period. Average year of birth and standard deviation was 1985 +/- 2.3 for the birth series, and 1912 +/- 14.4 for the death series. Then the surname distributions, in two independent samples at an average distance of 73 years, were compared. It was observed that random isonymy within series, which depends on the shape of the distribution, stays fairly constant at three generations of distance, indicating near-equilibrium of surname turnover. The migrational contribution is indicated by the significant decrease of random isonymy between series, measured with the method of Lasker. It was also observed that immigrants to Ferrara have a life-span significantly longer than people born in the town.
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Papers by Roberto Barale