SUMMARY The complete genome sequence of leek yellow stripe virus (LYSV) isolate AC-50 from garlic... more SUMMARY The complete genome sequence of leek yellow stripe virus (LYSV) isolate AC-50 from garlic in India was determined and the predicted amino acid (aa) sequence was analyzed. The LYSV RNA genome is 10,131 nucleotides (nt) long excluding the poly(A) tail (KP168261) and has a large open reading frame (ORF) encoding a putative poly-protein of 3152 aa with conserved motifs typical of members of the genus Potyvirus in the family Potyviridae. The pretty interesting Potyviridae ORF (PIPO) coding region was identified in the P3 coding region. LYSV isolate AC-50 shares maximum nt and aa sequence identity of 79.9% and 87.2% with a LYSV isolate from Australia (HQ258895) at the full genome and polyprotein level, respectively, and clusters with clade II isolates from China, Mexico, Aus-tralia, Brazil and Spain. The P1 coding region of isolate AC-50 was highly variable with an identity range of 48.9-70.9% with other LYSV isolates and a deletion of 204 nt compared to Japanese and Australian is...
Shallot virus X (ShVX), a type species in the genus Allexivirus of the family Alfaflexiviridae ha... more Shallot virus X (ShVX), a type species in the genus Allexivirus of the family Alfaflexiviridae has been associated with shallot plants in India and other shallot growing countries like Russia, Germany, Netherland, and New Zealand. Coat protein (CP) and nucleic acid binding protein (NB) region of the virus was obtained by reverse transcriptase polymerase chain reaction from scales leaves of
Indian garlic is infected with Onion yellow dwarf virus (OYDV), Shallot latent virus (SLV), Garli... more Indian garlic is infected with Onion yellow dwarf virus (OYDV), Shallot latent virus (SLV), Garlic common latent virus (GarCLV) and allexiviruses. Identity and distribution of garlic viruses in various garlic accessions from different geographical regions of India were investigated. OYDV and allexiviruses were observed in all the garlic accessions, while SLV and GarCLV were observed only in a few accessions. A multiplex reverse transcription (RT)-PCR method was developed for the simultaneous detection and identification of OYDV, SLV, GarCLV and Allexivirus infecting garlic accessions in India. This multiplex protocol standardized in this study will be useful in indexing of garlic viruses and production of virus free seed material.
... Editor-in-Chief: R. Michael Davis Published by The American Phytopathological Society. ... R.... more ... Editor-in-Chief: R. Michael Davis Published by The American Phytopathological Society. ... R. Manimekalai, VP Soumya, and R. Sathish Kumar, Central Plantation Crops Research Institute ... Nadu, India; K. Reddy, Indian Institute of Horticultural Research, IIHR, Bengaluru, India; GV ...
Reduced seed production in onion is associated with Onion yellow dwarf virus (OYDV), a filamentou... more Reduced seed production in onion is associated with Onion yellow dwarf virus (OYDV), a filamentous Potyvirus. Onion is also infected with other filamentous virus particles suspected to be Allexivirus. RT-PCR was used to detect mixed infection of both the viruses in leaves and bulbs. A duplex RT-PCR was developed, which simultaneously detected the presence of these two viruses in winter (Rabi) onion bulb. In summer (Kharif) onion bulbs only Allexivirus was detected. The absence of OYDV in summer crop is discussed. The sequencing of RT-PCR amplified products confirmed the identity of OYDV and Allexivirus, the latter showing closer identity to Garlic virus C (GVC)/Garlic mite-borne mosaic virus. This makes the first detection of an Allexivirus in onion crop in India. The duplex RT-PCR to detect these viruses (OYDV and Allexivirus) would be an improvement for indexing of viruses in onion bulbs for seed production.
During August 2004, symptoms resembling a virus disease were observed in commercial cultivation o... more During August 2004, symptoms resembling a virus disease were observed in commercial cultivation of sponge gourd (Luffa cylindrica (L.) M. Roem. [cv. Chikni]) in Pune, India. Affected plants exhibited mosaic mottling, vein banding, and downward marginal curling on leaves. The incidence ...
A virus inhibitor in Celosia was shown to have moderate thermal stability and resistance to dilut... more A virus inhibitor in Celosia was shown to have moderate thermal stability and resistance to dilution and drying. It was non-dialysable and non-sedimentable and showed glycoproteinaceous characteristics with a molecular weight between 21Â 000 and 22Â 000. The inhibitory activity was not reversible by simultaneous application of actinomycin-D to the leaf surface of Nicotiana glutinosa and the virus was fully recovered from a mixture of tobacco mosaic virus and inhibitor, indicating that the inhibitor did not appear to act on the virus directly but interfered with virus establishment in the host.
SUMMARY The complete genome sequence of leek yellow stripe virus (LYSV) isolate AC-50 from garlic... more SUMMARY The complete genome sequence of leek yellow stripe virus (LYSV) isolate AC-50 from garlic in India was determined and the predicted amino acid (aa) sequence was analyzed. The LYSV RNA genome is 10,131 nucleotides (nt) long excluding the poly(A) tail (KP168261) and has a large open reading frame (ORF) encoding a putative poly-protein of 3152 aa with conserved motifs typical of members of the genus Potyvirus in the family Potyviridae. The pretty interesting Potyviridae ORF (PIPO) coding region was identified in the P3 coding region. LYSV isolate AC-50 shares maximum nt and aa sequence identity of 79.9% and 87.2% with a LYSV isolate from Australia (HQ258895) at the full genome and polyprotein level, respectively, and clusters with clade II isolates from China, Mexico, Aus-tralia, Brazil and Spain. The P1 coding region of isolate AC-50 was highly variable with an identity range of 48.9-70.9% with other LYSV isolates and a deletion of 204 nt compared to Japanese and Australian is...
Shallot virus X (ShVX), a type species in the genus Allexivirus of the family Alfaflexiviridae ha... more Shallot virus X (ShVX), a type species in the genus Allexivirus of the family Alfaflexiviridae has been associated with shallot plants in India and other shallot growing countries like Russia, Germany, Netherland, and New Zealand. Coat protein (CP) and nucleic acid binding protein (NB) region of the virus was obtained by reverse transcriptase polymerase chain reaction from scales leaves of
Indian garlic is infected with Onion yellow dwarf virus (OYDV), Shallot latent virus (SLV), Garli... more Indian garlic is infected with Onion yellow dwarf virus (OYDV), Shallot latent virus (SLV), Garlic common latent virus (GarCLV) and allexiviruses. Identity and distribution of garlic viruses in various garlic accessions from different geographical regions of India were investigated. OYDV and allexiviruses were observed in all the garlic accessions, while SLV and GarCLV were observed only in a few accessions. A multiplex reverse transcription (RT)-PCR method was developed for the simultaneous detection and identification of OYDV, SLV, GarCLV and Allexivirus infecting garlic accessions in India. This multiplex protocol standardized in this study will be useful in indexing of garlic viruses and production of virus free seed material.
... Editor-in-Chief: R. Michael Davis Published by The American Phytopathological Society. ... R.... more ... Editor-in-Chief: R. Michael Davis Published by The American Phytopathological Society. ... R. Manimekalai, VP Soumya, and R. Sathish Kumar, Central Plantation Crops Research Institute ... Nadu, India; K. Reddy, Indian Institute of Horticultural Research, IIHR, Bengaluru, India; GV ...
Reduced seed production in onion is associated with Onion yellow dwarf virus (OYDV), a filamentou... more Reduced seed production in onion is associated with Onion yellow dwarf virus (OYDV), a filamentous Potyvirus. Onion is also infected with other filamentous virus particles suspected to be Allexivirus. RT-PCR was used to detect mixed infection of both the viruses in leaves and bulbs. A duplex RT-PCR was developed, which simultaneously detected the presence of these two viruses in winter (Rabi) onion bulb. In summer (Kharif) onion bulbs only Allexivirus was detected. The absence of OYDV in summer crop is discussed. The sequencing of RT-PCR amplified products confirmed the identity of OYDV and Allexivirus, the latter showing closer identity to Garlic virus C (GVC)/Garlic mite-borne mosaic virus. This makes the first detection of an Allexivirus in onion crop in India. The duplex RT-PCR to detect these viruses (OYDV and Allexivirus) would be an improvement for indexing of viruses in onion bulbs for seed production.
During August 2004, symptoms resembling a virus disease were observed in commercial cultivation o... more During August 2004, symptoms resembling a virus disease were observed in commercial cultivation of sponge gourd (Luffa cylindrica (L.) M. Roem. [cv. Chikni]) in Pune, India. Affected plants exhibited mosaic mottling, vein banding, and downward marginal curling on leaves. The incidence ...
A virus inhibitor in Celosia was shown to have moderate thermal stability and resistance to dilut... more A virus inhibitor in Celosia was shown to have moderate thermal stability and resistance to dilution and drying. It was non-dialysable and non-sedimentable and showed glycoproteinaceous characteristics with a molecular weight between 21Â 000 and 22Â 000. The inhibitory activity was not reversible by simultaneous application of actinomycin-D to the leaf surface of Nicotiana glutinosa and the virus was fully recovered from a mixture of tobacco mosaic virus and inhibitor, indicating that the inhibitor did not appear to act on the virus directly but interfered with virus establishment in the host.
Uploads
Papers by V Baranwal