Abstract Water deficit is one of the most serious environmental stress that affects the various p... more Abstract Water deficit is one of the most serious environmental stress that affects the various physiological and biochemical reactions in plants. Applying certain elicitors such as biostimulants in stress conditions can mitigate the negative impact of stress on crop. Therefore, a factorial experiment based on randomized complete block design with three replications and three factors was conducted under greenhouse conditions. The factors include irrigation regime (irrigation based on 100 % and 40 % field capacity), ecotype (Ardestan, Qazvin and Kermanshah), and biostimulant application (without biostimulant, application of arbuscular mycorrhiza fungi (AMF) that added to the soil at planting time and application of seaweeds extract (SWE) with irrigation water. The results indicated that water deficit stress (40 % of field capacity) decreased most of the measured traits especially K/Na, plant fresh weight and seed number. However applying biostimulants increased many traits compared to control conditions. AMF and SWE had the most effect on carotenoids, seed yield and plant fresh weight. Root fresh weight, plant fresh weight and seed yield were higher in the mycorrhizal incubated plants than SWE treated plants. Under both, well-watered and water deficit conditions Qazvin ecotype with application of AMF had the highest grain yield and plant fresh weight compared to other treatments. In addition, the amount of potassium (K) and calcium (Ca), chlorophyll a, total chlorophyll, stomatal width and seed number in this treatment were higher than others. Generally, AMF and SWE showed more positive effect on Qazvin ecotype than other studied ecotypes.
As part of ongoing project on screening plants used in Iranian folk medicine to treatment of diab... more As part of ongoing project on screening plants used in Iranian folk medicine to treatment of diabetes, α-glucosidase inhibition activities of Rosa canina extracts have been tested. The acetone extract of the plant exhibited significant inhibition with IC50 value of 0.3 μg/ml. The enzyme based assay guided fractionation of the acetone extract led to the isolation of daucosterol (1) and D-glucono-1,4-lactone (2), as highly active αglucosidase inhibitors. Their structures were determined by 1 Hand 13 C-NMR spectroscopic evidences. The IC50 values of daucosterol and D-glucono-1,4-lactone on yeast α-glucosidase were 13.3 and 6.5 μM, respectively, while IC50 of acarbose was 16.1 μM, as a positive control. The Lineweaver-Burk plots analysis elucidated that both of the compounds inhibited the enzyme competitively. The study suggests that isolated compounds can be good candidates as α-glucosidase inhibitors and provide strong rationale for more in vivo studies.
Aims: Helicobacter pylori infection spreads widely in the world and has several complications inc... more Aims: Helicobacter pylori infection spreads widely in the world and has several complications including gastric cancer. The aim of this work is to investigate the antimicrobial properties of Valeriana officinalis L., Satureja bachtiarica and Thymus daenensis extracts against H. pylori. Methods: The disk diffusion test was used in this experiment. Methanolic extract was isolated from Valeriana officinalis L., Satureja bachtiarica and Thymus daenensis. Ten clinical isolates of H. pylori were used in this experiment, which was obtained at Alzahra hospital, Tehran, Iran from individual patients with gastrointestinal disorders. H. pylori strains were identified using the checking of bacterial growth (0.5 – 1 mm), Gram staining, urease test, catalase test and the drug resistance standard was performed on them. To determine the MIC of the extracts, disk diffusion (Kirby-Bauer) test in agar was used. Results: The clinical strains showed the highest susceptibility to tetracycline (10%). The ...
The genus Salvia includes an enormous assemblage of nearly 1,000 species dispersed around the wor... more The genus Salvia includes an enormous assemblage of nearly 1,000 species dispersed around the world. Due to possible threats to this genus, there is an immediate requirement to evaluate the diversity of its wild populations. ISSR and RAPD molecular techniques were used to evaluate the genetic relationships among twenty-one ecotypes of eight Salvia species. Amplification of genomic DNA using 23 primers (15 RAPD and eight ISSR) produced 280 bands, of which 91% were polymorphic. The results of marker parameters showed no clear difference between two marker systems. It was generally observed that both ISSR and RAPD markers had similar efficiency in detecting genetic polymorphisms with remarkable ability to differentiate the closely related ecotypes of Salvia. Nei’s similarity coefficients for these techniques ranged from 0.48 to 0.98. Based on the results of clustering, PCoA and AMOVA, the genetic diversity between and within species was confirmed. So, conservation and domestication of ...
Abstract Water deficit is one of the most serious environmental stress that affects the various p... more Abstract Water deficit is one of the most serious environmental stress that affects the various physiological and biochemical reactions in plants. Applying certain elicitors such as biostimulants in stress conditions can mitigate the negative impact of stress on crop. Therefore, a factorial experiment based on randomized complete block design with three replications and three factors was conducted under greenhouse conditions. The factors include irrigation regime (irrigation based on 100 % and 40 % field capacity), ecotype (Ardestan, Qazvin and Kermanshah), and biostimulant application (without biostimulant, application of arbuscular mycorrhiza fungi (AMF) that added to the soil at planting time and application of seaweeds extract (SWE) with irrigation water. The results indicated that water deficit stress (40 % of field capacity) decreased most of the measured traits especially K/Na, plant fresh weight and seed number. However applying biostimulants increased many traits compared to control conditions. AMF and SWE had the most effect on carotenoids, seed yield and plant fresh weight. Root fresh weight, plant fresh weight and seed yield were higher in the mycorrhizal incubated plants than SWE treated plants. Under both, well-watered and water deficit conditions Qazvin ecotype with application of AMF had the highest grain yield and plant fresh weight compared to other treatments. In addition, the amount of potassium (K) and calcium (Ca), chlorophyll a, total chlorophyll, stomatal width and seed number in this treatment were higher than others. Generally, AMF and SWE showed more positive effect on Qazvin ecotype than other studied ecotypes.
As part of ongoing project on screening plants used in Iranian folk medicine to treatment of diab... more As part of ongoing project on screening plants used in Iranian folk medicine to treatment of diabetes, α-glucosidase inhibition activities of Rosa canina extracts have been tested. The acetone extract of the plant exhibited significant inhibition with IC50 value of 0.3 μg/ml. The enzyme based assay guided fractionation of the acetone extract led to the isolation of daucosterol (1) and D-glucono-1,4-lactone (2), as highly active αglucosidase inhibitors. Their structures were determined by 1 Hand 13 C-NMR spectroscopic evidences. The IC50 values of daucosterol and D-glucono-1,4-lactone on yeast α-glucosidase were 13.3 and 6.5 μM, respectively, while IC50 of acarbose was 16.1 μM, as a positive control. The Lineweaver-Burk plots analysis elucidated that both of the compounds inhibited the enzyme competitively. The study suggests that isolated compounds can be good candidates as α-glucosidase inhibitors and provide strong rationale for more in vivo studies.
Aims: Helicobacter pylori infection spreads widely in the world and has several complications inc... more Aims: Helicobacter pylori infection spreads widely in the world and has several complications including gastric cancer. The aim of this work is to investigate the antimicrobial properties of Valeriana officinalis L., Satureja bachtiarica and Thymus daenensis extracts against H. pylori. Methods: The disk diffusion test was used in this experiment. Methanolic extract was isolated from Valeriana officinalis L., Satureja bachtiarica and Thymus daenensis. Ten clinical isolates of H. pylori were used in this experiment, which was obtained at Alzahra hospital, Tehran, Iran from individual patients with gastrointestinal disorders. H. pylori strains were identified using the checking of bacterial growth (0.5 – 1 mm), Gram staining, urease test, catalase test and the drug resistance standard was performed on them. To determine the MIC of the extracts, disk diffusion (Kirby-Bauer) test in agar was used. Results: The clinical strains showed the highest susceptibility to tetracycline (10%). The ...
The genus Salvia includes an enormous assemblage of nearly 1,000 species dispersed around the wor... more The genus Salvia includes an enormous assemblage of nearly 1,000 species dispersed around the world. Due to possible threats to this genus, there is an immediate requirement to evaluate the diversity of its wild populations. ISSR and RAPD molecular techniques were used to evaluate the genetic relationships among twenty-one ecotypes of eight Salvia species. Amplification of genomic DNA using 23 primers (15 RAPD and eight ISSR) produced 280 bands, of which 91% were polymorphic. The results of marker parameters showed no clear difference between two marker systems. It was generally observed that both ISSR and RAPD markers had similar efficiency in detecting genetic polymorphisms with remarkable ability to differentiate the closely related ecotypes of Salvia. Nei’s similarity coefficients for these techniques ranged from 0.48 to 0.98. Based on the results of clustering, PCoA and AMOVA, the genetic diversity between and within species was confirmed. So, conservation and domestication of ...
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