Several dams have been constructed in Uttarakhand. In the present study an investigation on imp... more Several dams have been constructed in Uttarakhand. In the present study an investigation on impact of dams on genetic diversity of native fish species Mastacembelus armatus at the Asan barrage on river Yamuna near Vikasnagar, Uttarakhand in India was done. Partial sequence of mitochondrial Cytochrome-b (Cyto-b) gene was used to determine the genetic variation in the population of Mastacembelus armatus. DNA was extracted from Mastacembelus armatus (n=33) samples, collected from river Yamuna and its tributary Asan from fin and fishes were released back in their habitat. Cytochrome c oxidase I (COI) was used to ascertain the species of fish along with morphometric characters. Analysis of 324 bp mtDNA fragment of Cyto-b revealed the presence of 06 haplotypes with nucleotide diversity, value ranged from 0.0172 to 0.0021 low pair wise Fst value was observed negative (-0.00125) when compared between Asan barrage and Kalsi site. No genetic subdivisions between the population were found ...
We explored fish fauna Ganga and Yamuna river in Uttarkashi district of Uttarakhand, to establish... more We explored fish fauna Ganga and Yamuna river in Uttarkashi district of Uttarakhand, to establish the molecular taxonomy database. A total of 133 samples were collected from various sampling sites along the entire stretch of rivers in district Uttarkashi. A region of cytochrome c-oxidase unit I (COI) gene (655 bp) was amplified using PCR and sequenced (DNA Barcoded). All the sequences have been uploaded into the NCBI GenBank (accession number assigned). Analysis of data generated showed that these 133 samples belonged to 22 species of 4 orders, 14 genera and 8 families. The genetic variability (K2P distance) distribution analysis was also carried out. The average mean distance of 22 species is 0.219 with 0.014 standard error. The mean genetic distance between 22 species ranged from 0.010%-0.362% while the mean genetic distance within the species ranged from 0.0006%0.0048%. The lowest pairwise genetic distance observed in Schizothorax sinuatus and Schizothorax progastus i.e. 0.010% w...
-electric power projects, etc., are being constructed leading to increase -d) range was between 0... more -electric power projects, etc., are being constructed leading to increase -d) range was between 0.3422 to 0.42. Various physicochemical parameters of ion. Population of Daniodevario tivity. Our study shows that there is an urgent need r bed mining, construction, habitat, Uttarakhand
The entire Himalayan region is well known as a global hotspot for biodiversity. Many workers have... more The entire Himalayan region is well known as a global hotspot for biodiversity. Many workers have documented different aspect of biodiversity. The Uttarakhand Himalaya region, a part of Himalayan system, is very rich in fresh water aquatic biodiversity because it has many fresh water streams & rivers within a short distance of 200 miles. All these fresh water bodies harbor diverse aquatic fauna with fishes being the most extensively studied. There are many fish species reported by many authors in Uttarakhand. Many of the fishes have similar morphological characters (morpho-metrics) and are difficult to identify. Attempts have been made to generate the DNA barcode of fishes but most of the attempt are limited to major rivers i.e. the Ganges and the Yamuna. Besides these two prominent river (the Ganges & the Yamuna), there are many supporting streams and small tributaries that are also inhabited by many species. No “long term” and “entire river scan” for fish species has ever been con...
Several dams have been constructed in Uttarakhand. In the present study an investigation on imp... more Several dams have been constructed in Uttarakhand. In the present study an investigation on impact of dams on genetic diversity of native fish species Mastacembelus armatus at the Asan barrage on river Yamuna near Vikasnagar, Uttarakhand in India was done. Partial sequence of mitochondrial Cytochrome-b (Cyto-b) gene was used to determine the genetic variation in the population of Mastacembelus armatus. DNA was extracted from Mastacembelus armatus (n=33) samples, collected from river Yamuna and its tributary Asan from fin and fishes were released back in their habitat. Cytochrome c oxidase I (COI) was used to ascertain the species of fish along with morphometric characters. Analysis of 324 bp mtDNA fragment of Cyto-b revealed the presence of 06 haplotypes with nucleotide diversity, value ranged from 0.0172 to 0.0021 low pair wise Fst value was observed negative (-0.00125) when compared between Asan barrage and Kalsi site. No genetic subdivisions between the population were found ...
We explored fish fauna Ganga and Yamuna river in Uttarkashi district of Uttarakhand, to establish... more We explored fish fauna Ganga and Yamuna river in Uttarkashi district of Uttarakhand, to establish the molecular taxonomy database. A total of 133 samples were collected from various sampling sites along the entire stretch of rivers in district Uttarkashi. A region of cytochrome c-oxidase unit I (COI) gene (655 bp) was amplified using PCR and sequenced (DNA Barcoded). All the sequences have been uploaded into the NCBI GenBank (accession number assigned). Analysis of data generated showed that these 133 samples belonged to 22 species of 4 orders, 14 genera and 8 families. The genetic variability (K2P distance) distribution analysis was also carried out. The average mean distance of 22 species is 0.219 with 0.014 standard error. The mean genetic distance between 22 species ranged from 0.010%-0.362% while the mean genetic distance within the species ranged from 0.0006%0.0048%. The lowest pairwise genetic distance observed in Schizothorax sinuatus and Schizothorax progastus i.e. 0.010% w...
-electric power projects, etc., are being constructed leading to increase -d) range was between 0... more -electric power projects, etc., are being constructed leading to increase -d) range was between 0.3422 to 0.42. Various physicochemical parameters of ion. Population of Daniodevario tivity. Our study shows that there is an urgent need r bed mining, construction, habitat, Uttarakhand
The entire Himalayan region is well known as a global hotspot for biodiversity. Many workers have... more The entire Himalayan region is well known as a global hotspot for biodiversity. Many workers have documented different aspect of biodiversity. The Uttarakhand Himalaya region, a part of Himalayan system, is very rich in fresh water aquatic biodiversity because it has many fresh water streams & rivers within a short distance of 200 miles. All these fresh water bodies harbor diverse aquatic fauna with fishes being the most extensively studied. There are many fish species reported by many authors in Uttarakhand. Many of the fishes have similar morphological characters (morpho-metrics) and are difficult to identify. Attempts have been made to generate the DNA barcode of fishes but most of the attempt are limited to major rivers i.e. the Ganges and the Yamuna. Besides these two prominent river (the Ganges & the Yamuna), there are many supporting streams and small tributaries that are also inhabited by many species. No “long term” and “entire river scan” for fish species has ever been con...
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