I am working in the area of Medical Biotechnology for the last several years with nearly 50 years of experience in teaching and research, academic administrator. I am giving my services as advisor to several companies in food industries and pharmaceutics. I have nearly 300 research papers published in journals of international repute, 10 patents awarded by US, India, Japan and European Union and 10 outstanding books on contemporary issues viz HIV, Cancer, Infectitous Diseases, instrumentation’s and protocols published by Soringer, CRC and Wiley.
... Titre du document / Document title. Mutation to resistance for virus AS-1 in the cyanobacteri... more ... Titre du document / Document title. Mutation to resistance for virus AS-1 in the cyanobacterium Anacystis nidulans Auteur(s) / Author(s). BISEN PS (1) ; SURABHI AUDHOLIA ; BHATNAGAR AK ; Affiliation(s) du ou des auteurs / Author(s) Affiliation(s). (1) Rani Durgawati univ. dep. ...
Ellagic acid is an antioxidant and an anti-proliferative compound present in fruits, nuts and veg... more Ellagic acid is an antioxidant and an anti-proliferative compound present in fruits, nuts and vegetables. In spite of evidences for anticancer activity in various cancer cell-lines, human cancer cells, the mechanistic role of ellagic acid is not conclusive enough to be recommended for a clinical use. The present review provides information about the chemopreventive role of ellagic acid in oral cancer and proposes molecular basis for ellagic acid’s inhibitory activity against oral cancer. We show that ellagic acid modulates growth of tumor cells through regulation of multiple cell signaling pathways including cell proliferation pathway (cyclin dependent kinase 2, cyclin A2, cyclin B1, cyclin D1, c-myc, PKCα), cell survival/apoptosis pathway (Bcl-XL, Bax, Caspase 9/3, Akt), tumor suppressor pathway (p53, p21), inflaming Metastasis pathways (IL-1 beta, TNF-α, matrix metalloproteinases 9/3, COX-2), angiogenesis pathways (VEGF), cell immortalization (TERT), NF-κβ.
Hepatotoxicity associated with isoniazid and rifampicin is one of the major impediments in antitu... more Hepatotoxicity associated with isoniazid and rifampicin is one of the major impediments in antituberculosis therapy. The present study explored the prophylactic and therapeutic efficacies of Spirulina maxima in isoniazid and rifampicin induced hepatic damage in a rat model. Hepatic damage induced in Wistar rats by isoniazid and rifampicin resulted in significant alterations in biomarkers of liver function, namely, bilirubin, aspartate transaminase, alanine transaminase, alkaline phosphatase, and oxidative stress markers such as superoxide dismutase, catalase, glutathione, and thiobarbituric acid reactive substances. Co-administration of Spirulina maxima along with antituberculosis drugs protected liver from hepatotoxicity due to isoniazid and rifampicin. Administration of Spirulina maxima consecutively for 2 weeks to hepatodamaged animals resulted in restoration of hepatic function as evident from normalization of serum markers of liver function. Thus, the present study revealed rem...
Chlorophytum borivilianum is a medicinal plant with unique healing and health improving propertie... more Chlorophytum borivilianum is a medicinal plant with unique healing and health improving properties. The effect of abiotic stress on this plant was studied under in vitro conditions. Both salinity and drought conditions affected all the morphological parameters and decreased growth performance. There was reduced metabolism in the tissues under stress condition. The contents of chlorophyll decreased while the proline and protein increased with the increasing concentration of NaCl and mannitol with time. The plantlets tolerant to different stress conditions were further analyzed by RAPD. Distint polymorphism was observed in the plantlets and regions related to the stress have been identified. Further work to determine the content of secondary metabolites and genes regulating the abiotic stress.
The metabolic syndrome (Met S) is comprised of various medical conditions that confer increased r... more The metabolic syndrome (Met S) is comprised of various medical conditions that confer increased risk of diabetes and cardiovascular disease. The pathophysiological components of MS include glucose abnormality, obesity or increased waist circumference, blood pressure, and hyperlipidemia. Methods: We studied the prevalence of the metabolic syndrome (MetS) in Gwalior- Chambal region of Central India, according to two definitions (NCEP- ATPIII and IDF); and we also examined these issues upon link between metabolic syndrome and diabetes. We conducted an examination survey on a sample representative of the general population aged 30-70 years, attended by 385 participants in which 275 males and 110 females. Conclusions: The prevalence of MetS increased markedly with age. According to the NCEP-ATPIII and IDF definitions, the prevalence of MetS was, 41.29% and 47.0% respectively. Approximately 65.15% of participants with diabetes also had MetS and the prevalence of MetS was approximately 7.7...
Lectins are structurally diverse, carbohydrate binding proteins that bind reversibly to specific ... more Lectins are structurally diverse, carbohydrate binding proteins that bind reversibly to specific mono- or oligosaccharides. Their abundance in the plant kingdom suggest that they have diverse roles to perform. They serve as recognition factor between symbiotic nitrogen fixing bacteria and host plants, as a deterrent to phytopathogens like fungi, insects, and animals, as storage protein and as an aid in sexual reproduction in Chlamydomonas, amongst others. The possible application of lectins as a factor in increasing soil fertility and as a biopesticide by genetically engineered organisms is yet to be fully explored by the biotechnologists. However, they are being used by the biomedical scientists and biochemists in blood typing and stimulation of cells for chromosome analysis and gene mapping, in cell separation, identification of complex glycoproteins and typing of bacteria. Cell targeting by lectins in cancer therapy is still in its infancy. This review gives an insight into the p...
Copper-induced changes in the urea uptake and urease activity have been investigated in the cyano... more Copper-induced changes in the urea uptake and urease activity have been investigated in the cyanobacteria Anabaena doliolum and Anacystis nidulans. Copper, at and above 5 mumol/L concentration, inhibited urea uptake and urease activity systems in both the cyanobacteria in a concentration dependent manner. However, the urea uptake and urease activity systems in A. nidulans appeared slightly more tolerant to copper than than of A. doliolum. The inhibitory effect of copper on urea uptake and urease activity was mitigated by sulphur containing amino acids (cystine and cysteine), however, methionine could not do so, indicating the involvement of sulfhydryl (-SH) groups in the assimilation of urea in cyanobacteria.
Diabetes mellitus is a metabolic disorder characterized by hyperglycaemia. Several natural produc... more Diabetes mellitus is a metabolic disorder characterized by hyperglycaemia. Several natural products have been isolated and identified to restore the complications of diabetes. Spirulina maxima is naturally occurring fresh water cyanobacterium, enriched with proteins and essential nutrients. The aim of the study was to determine whether S. maxima could serve as a therapeutic agent to correct metabolic abnormalities induced by excessive fructose administration in Wistar rats. Oral administration of 10 per cent fructose solution to Wistar rats (n = 5 in each group) for 30 days resulted in hyperglycaemia and hyperlipidaemia. Aqueous suspension of S. maxima (5 or 10%) was also administered orally once daily for 30 days. The therapeutic potential of the preparation with reference to metformin (500 mg/kg) was assessed by monitoring various biochemical parameters at 10 day intervals during the course of therapy and at the end of 30 days S. maxima administration. Significant (P<0.001) red...
... Titre du document / Document title. Mutation to resistance for virus AS-1 in the cyanobacteri... more ... Titre du document / Document title. Mutation to resistance for virus AS-1 in the cyanobacterium Anacystis nidulans Auteur(s) / Author(s). BISEN PS (1) ; SURABHI AUDHOLIA ; BHATNAGAR AK ; Affiliation(s) du ou des auteurs / Author(s) Affiliation(s). (1) Rani Durgawati univ. dep. ...
Ellagic acid is an antioxidant and an anti-proliferative compound present in fruits, nuts and veg... more Ellagic acid is an antioxidant and an anti-proliferative compound present in fruits, nuts and vegetables. In spite of evidences for anticancer activity in various cancer cell-lines, human cancer cells, the mechanistic role of ellagic acid is not conclusive enough to be recommended for a clinical use. The present review provides information about the chemopreventive role of ellagic acid in oral cancer and proposes molecular basis for ellagic acid’s inhibitory activity against oral cancer. We show that ellagic acid modulates growth of tumor cells through regulation of multiple cell signaling pathways including cell proliferation pathway (cyclin dependent kinase 2, cyclin A2, cyclin B1, cyclin D1, c-myc, PKCα), cell survival/apoptosis pathway (Bcl-XL, Bax, Caspase 9/3, Akt), tumor suppressor pathway (p53, p21), inflaming Metastasis pathways (IL-1 beta, TNF-α, matrix metalloproteinases 9/3, COX-2), angiogenesis pathways (VEGF), cell immortalization (TERT), NF-κβ.
Hepatotoxicity associated with isoniazid and rifampicin is one of the major impediments in antitu... more Hepatotoxicity associated with isoniazid and rifampicin is one of the major impediments in antituberculosis therapy. The present study explored the prophylactic and therapeutic efficacies of Spirulina maxima in isoniazid and rifampicin induced hepatic damage in a rat model. Hepatic damage induced in Wistar rats by isoniazid and rifampicin resulted in significant alterations in biomarkers of liver function, namely, bilirubin, aspartate transaminase, alanine transaminase, alkaline phosphatase, and oxidative stress markers such as superoxide dismutase, catalase, glutathione, and thiobarbituric acid reactive substances. Co-administration of Spirulina maxima along with antituberculosis drugs protected liver from hepatotoxicity due to isoniazid and rifampicin. Administration of Spirulina maxima consecutively for 2 weeks to hepatodamaged animals resulted in restoration of hepatic function as evident from normalization of serum markers of liver function. Thus, the present study revealed rem...
Chlorophytum borivilianum is a medicinal plant with unique healing and health improving propertie... more Chlorophytum borivilianum is a medicinal plant with unique healing and health improving properties. The effect of abiotic stress on this plant was studied under in vitro conditions. Both salinity and drought conditions affected all the morphological parameters and decreased growth performance. There was reduced metabolism in the tissues under stress condition. The contents of chlorophyll decreased while the proline and protein increased with the increasing concentration of NaCl and mannitol with time. The plantlets tolerant to different stress conditions were further analyzed by RAPD. Distint polymorphism was observed in the plantlets and regions related to the stress have been identified. Further work to determine the content of secondary metabolites and genes regulating the abiotic stress.
The metabolic syndrome (Met S) is comprised of various medical conditions that confer increased r... more The metabolic syndrome (Met S) is comprised of various medical conditions that confer increased risk of diabetes and cardiovascular disease. The pathophysiological components of MS include glucose abnormality, obesity or increased waist circumference, blood pressure, and hyperlipidemia. Methods: We studied the prevalence of the metabolic syndrome (MetS) in Gwalior- Chambal region of Central India, according to two definitions (NCEP- ATPIII and IDF); and we also examined these issues upon link between metabolic syndrome and diabetes. We conducted an examination survey on a sample representative of the general population aged 30-70 years, attended by 385 participants in which 275 males and 110 females. Conclusions: The prevalence of MetS increased markedly with age. According to the NCEP-ATPIII and IDF definitions, the prevalence of MetS was, 41.29% and 47.0% respectively. Approximately 65.15% of participants with diabetes also had MetS and the prevalence of MetS was approximately 7.7...
Lectins are structurally diverse, carbohydrate binding proteins that bind reversibly to specific ... more Lectins are structurally diverse, carbohydrate binding proteins that bind reversibly to specific mono- or oligosaccharides. Their abundance in the plant kingdom suggest that they have diverse roles to perform. They serve as recognition factor between symbiotic nitrogen fixing bacteria and host plants, as a deterrent to phytopathogens like fungi, insects, and animals, as storage protein and as an aid in sexual reproduction in Chlamydomonas, amongst others. The possible application of lectins as a factor in increasing soil fertility and as a biopesticide by genetically engineered organisms is yet to be fully explored by the biotechnologists. However, they are being used by the biomedical scientists and biochemists in blood typing and stimulation of cells for chromosome analysis and gene mapping, in cell separation, identification of complex glycoproteins and typing of bacteria. Cell targeting by lectins in cancer therapy is still in its infancy. This review gives an insight into the p...
Copper-induced changes in the urea uptake and urease activity have been investigated in the cyano... more Copper-induced changes in the urea uptake and urease activity have been investigated in the cyanobacteria Anabaena doliolum and Anacystis nidulans. Copper, at and above 5 mumol/L concentration, inhibited urea uptake and urease activity systems in both the cyanobacteria in a concentration dependent manner. However, the urea uptake and urease activity systems in A. nidulans appeared slightly more tolerant to copper than than of A. doliolum. The inhibitory effect of copper on urea uptake and urease activity was mitigated by sulphur containing amino acids (cystine and cysteine), however, methionine could not do so, indicating the involvement of sulfhydryl (-SH) groups in the assimilation of urea in cyanobacteria.
Diabetes mellitus is a metabolic disorder characterized by hyperglycaemia. Several natural produc... more Diabetes mellitus is a metabolic disorder characterized by hyperglycaemia. Several natural products have been isolated and identified to restore the complications of diabetes. Spirulina maxima is naturally occurring fresh water cyanobacterium, enriched with proteins and essential nutrients. The aim of the study was to determine whether S. maxima could serve as a therapeutic agent to correct metabolic abnormalities induced by excessive fructose administration in Wistar rats. Oral administration of 10 per cent fructose solution to Wistar rats (n = 5 in each group) for 30 days resulted in hyperglycaemia and hyperlipidaemia. Aqueous suspension of S. maxima (5 or 10%) was also administered orally once daily for 30 days. The therapeutic potential of the preparation with reference to metformin (500 mg/kg) was assessed by monitoring various biochemical parameters at 10 day intervals during the course of therapy and at the end of 30 days S. maxima administration. Significant (P<0.001) red...
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