Alprazolam is the most commonly used allopathic remedy for the management of anxiety. Several dru... more Alprazolam is the most commonly used allopathic remedy for the management of anxiety. Several drugs like Withania, Valerian & Centella are the leading herbs for the management of anxiety. Magnesium hydroxide when given alone shows laxative property at all does but if it is given along with Licorice it enhances CNS activity of magnesium salt and produces hypnosis instead of laxation. Hence in the present study attempts have been made to screen possible synergistic potentiating anti-anxiety activity of Withania and Alprazolam with Licorice in elevated plus maze and dark light exploration model. In elevated plus maze model time spent in open arm and number of cross entries were increased significantly when Withania extract or Alprazolam were given along with Licorice. To conclude, if Withania or Alprazolam is given in combination with Licorice, there is enhancement of their anti-anxiety activities in mice. The synergism in the anti-anxiety activity may be due to differences in their me...
Background: Anxiety disorders are the most common of emotional disorders, affecting more than 20 ... more Background: Anxiety disorders are the most common of emotional disorders, affecting more than 20 million people annually. Sarpagandha Ghanvati is a classical Ayurvedic polyherbal formulation prescribed in conditions of insomnia, hysteria, and is used as an anxiolytic agent. Standardization and quality control are the two major issues that need to be addressed for herbal formulations, especially those containing multiple herbal ingredients. Objective: An HPTLC method was developed for the simultaneous quantification of reserpine, atropine, and piperine from Sarpagandha Ghanvati containing Rauwolfia serpentine (root), Hyoscyamus niger (seed), and Piper longum (root and stem). Methods: The marker compounds were effectively resolved on a silica gel G TLC plate using toluene–ethyl acetate–diethyl amine (7+2+1, v/v) as the mobile phase. The detected wavelengths for reserpine, atropine, and piperine were 269, 220, and 254 nm, respectively. The method was validated as per the International ...
JPC - Journal of Planar Chromatography - Modern TLC, 2014
ABSTRACT he fruit of Piper longum Linn. (family: Piperaceae), known as pippali in India, is a rep... more ABSTRACT he fruit of Piper longum Linn. (family: Piperaceae), known as pippali in India, is a reputed drug of Ayurveda (the Indian system of medicine). It contains many amides of piperic acid among which piperine and piperlongumine are important from biological point of view. In the present work, we quantified these two marker compounds from the fruits of P. longum by high-performance thin-layer chromatography (HPTLC) with densitometry. The method was found to be precise. Relative standard deviation (RSD) values for intra-day analyses were in the range of 1.05 to1.07% (piperine) and 1.12 to 1.16% (piperlongumine) and, for inter-day analyses, the values were in the range of 1.11 to 1.21% (piperine) and 1.02 to1.18% (piperlongumine). Instrumental RSD values were 0.64 and 0.78% for piperine and piperlongumine, respectively. Accuracy of the method was evaluated by carrying a recovery study at three different levels for the two compounds. Average recoveries were found to be 99.23% for piperine and 99.26% for piperlongumine. P. longum fruits obtained from Bombay market showed 0.213 ± 0.009% w/w piperine and 0.364 ± 0.014% w/w piperlongumine when analyzed by the proposed method. Similarly, sample fruits obtained from Ahmedabad market had 0.85 ± 0.026% piperine and 2.70 ± 0.065% of piperlongumine. This TLC-densitometric method was found to be simple, precise, specific, sensitive, and accurate. So it can be used in routine quality control for the simultaneous analysis of piperine and piperlongumine from P. longum fruits and piperine alone from Piper nigrum fruits
International Journal of Current Pharmaceutical Research, 2017
Objective: To develop and validate a simple, precise, selective, and accurate high-performance th... more Objective: To develop and validate a simple, precise, selective, and accurate high-performance thin layer chromatographic method for simultaneous densitometric analysis of glycyrrhetic acid and solasodine in the polyherbal formulation. Methods: The method was developed using HPTLC silica gel GF 254 precoated aluminium plate as the stationary phase and Chloroform: Methanol (9:1 v/v) as the mobile phase. Quantization of glycyrrhetic acid was achieved by determining the area under the curve at 267 nm using CAMAG TLC Scanner and CATS 3 software. Since the structure of solasodine lacks conjugated double bond, it does not give any fluorescence either in the absorbance mode or reflectance mode hence solasodine was derivatized using 0.5%anisaldehyde sulphuric acids which gave a bluish spot as seen on TLC plate. These spots were scanned at 546 nm wavelength using CAMAG TLC Scanner and CATS 3 software. Results: The retention factor for glycyrrhetic acid and solasodine were found to be 0.52±0....
Alprazolam is the most commonly used allopathic remedy for the management of anxiety. Several dru... more Alprazolam is the most commonly used allopathic remedy for the management of anxiety. Several drugs like Withania, Valerian & Centella are the leading herbs for the management of anxiety. Magnesium hydroxide when given alone shows laxative property at all does but if it is given along with Licorice it enhances CNS activity of magnesium salt and produces hypnosis instead of laxation. Hence in the present study attempts have been made to screen possible synergistic potentiating anti-anxiety activity of Withania and Alprazolam with Licorice in elevated plus maze and dark light exploration model. In elevated plus maze model time spent in open arm and number of cross entries were increased significantly when Withania extract or Alprazolam were given along with Licorice. To conclude, if Withania or Alprazolam is given in combination with Licorice, there is enhancement of their anti-anxiety activities in mice. The synergism in the anti-anxiety activity may be due to differences in their me...
Background: Anxiety disorders are the most common of emotional disorders, affecting more than 20 ... more Background: Anxiety disorders are the most common of emotional disorders, affecting more than 20 million people annually. Sarpagandha Ghanvati is a classical Ayurvedic polyherbal formulation prescribed in conditions of insomnia, hysteria, and is used as an anxiolytic agent. Standardization and quality control are the two major issues that need to be addressed for herbal formulations, especially those containing multiple herbal ingredients. Objective: An HPTLC method was developed for the simultaneous quantification of reserpine, atropine, and piperine from Sarpagandha Ghanvati containing Rauwolfia serpentine (root), Hyoscyamus niger (seed), and Piper longum (root and stem). Methods: The marker compounds were effectively resolved on a silica gel G TLC plate using toluene–ethyl acetate–diethyl amine (7+2+1, v/v) as the mobile phase. The detected wavelengths for reserpine, atropine, and piperine were 269, 220, and 254 nm, respectively. The method was validated as per the International ...
JPC - Journal of Planar Chromatography - Modern TLC, 2014
ABSTRACT he fruit of Piper longum Linn. (family: Piperaceae), known as pippali in India, is a rep... more ABSTRACT he fruit of Piper longum Linn. (family: Piperaceae), known as pippali in India, is a reputed drug of Ayurveda (the Indian system of medicine). It contains many amides of piperic acid among which piperine and piperlongumine are important from biological point of view. In the present work, we quantified these two marker compounds from the fruits of P. longum by high-performance thin-layer chromatography (HPTLC) with densitometry. The method was found to be precise. Relative standard deviation (RSD) values for intra-day analyses were in the range of 1.05 to1.07% (piperine) and 1.12 to 1.16% (piperlongumine) and, for inter-day analyses, the values were in the range of 1.11 to 1.21% (piperine) and 1.02 to1.18% (piperlongumine). Instrumental RSD values were 0.64 and 0.78% for piperine and piperlongumine, respectively. Accuracy of the method was evaluated by carrying a recovery study at three different levels for the two compounds. Average recoveries were found to be 99.23% for piperine and 99.26% for piperlongumine. P. longum fruits obtained from Bombay market showed 0.213 ± 0.009% w/w piperine and 0.364 ± 0.014% w/w piperlongumine when analyzed by the proposed method. Similarly, sample fruits obtained from Ahmedabad market had 0.85 ± 0.026% piperine and 2.70 ± 0.065% of piperlongumine. This TLC-densitometric method was found to be simple, precise, specific, sensitive, and accurate. So it can be used in routine quality control for the simultaneous analysis of piperine and piperlongumine from P. longum fruits and piperine alone from Piper nigrum fruits
International Journal of Current Pharmaceutical Research, 2017
Objective: To develop and validate a simple, precise, selective, and accurate high-performance th... more Objective: To develop and validate a simple, precise, selective, and accurate high-performance thin layer chromatographic method for simultaneous densitometric analysis of glycyrrhetic acid and solasodine in the polyherbal formulation. Methods: The method was developed using HPTLC silica gel GF 254 precoated aluminium plate as the stationary phase and Chloroform: Methanol (9:1 v/v) as the mobile phase. Quantization of glycyrrhetic acid was achieved by determining the area under the curve at 267 nm using CAMAG TLC Scanner and CATS 3 software. Since the structure of solasodine lacks conjugated double bond, it does not give any fluorescence either in the absorbance mode or reflectance mode hence solasodine was derivatized using 0.5%anisaldehyde sulphuric acids which gave a bluish spot as seen on TLC plate. These spots were scanned at 546 nm wavelength using CAMAG TLC Scanner and CATS 3 software. Results: The retention factor for glycyrrhetic acid and solasodine were found to be 0.52±0....
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