Zhonghua bing li xue za zhi Chinese journal of pathology, 1997
To analyse the pathomorphology and the clinical biological behavior of pilomatrix carcinoma as we... more To analyse the pathomorphology and the clinical biological behavior of pilomatrix carcinoma as well as to clarify the main points of diagnosis and differential diagnosis. A review of the clinicopathological, histochemical and immunohistochemical features of 15 pilomatrix carcinomas a well as their clinical data was made. Of the 15 cases, 6 were male and 9 female, aged 11 to 83 (mean age 46 years). The tumor diameter varied from 1.2 cm to 8.0 cm (mean 3.5 cm). Histologically, pilomatrix carcinomas are characterized by sheets and islands of proliferating atypical baseloid cells with an infiltrating border. Transition to squamous, clear and ghost cell are often observed. The trichohyalin granules and tricholin-like acidophilic bodies are seen in tumor cells. Follow-up on 13 cases revealed local recurrence in 7 cases with multiple recurrence in 2 cases. One died of liver metastasis and one died of extensive local spread of the tumor. Pilomatrix carcinoma is a malignant tumor which usual...
Die vorliegende Erfindung betrifft eine wassrige Losung fur die intravenose Infusion enthaltend C... more Die vorliegende Erfindung betrifft eine wassrige Losung fur die intravenose Infusion enthaltend Curcumin und/oder ein oder mehrere Curcuminderivate, Dimethylsulfoxid, einen oder mehrere Losungsvermittler, Natriumselenit, einen Puffer in einem wassrigen Infusionsmedium sowie ein Konzentrat zu ihrer Herstellung enthaltend Curcumin und/oder ein oder mehrere Curcuminderivate, Dimethylsulfoxid, einen oder mehrere Losungsvermittler und Natriumselenit.
The resin of the frankincense tree contains therapeutically effective boswellic acids (BA). Four ... more The resin of the frankincense tree contains therapeutically effective boswellic acids (BA). Four predominant BA selectively inhibit the synthesis of Leucotrienes (LT). A variety of chronic diseases is predominantly accompanied by excessive formation of LT and hence support and prolong the inflammatory process. Starting from the arachidonic acid, the inflammatory and pain cascade is interrupted without significant side effects. Even on the basis of long-term intake, the initial effects are maintained. Indications for a frankincense therapy are all chronic inflammatory and allergic diseases. A different pathway of activity involves the Inhibition of topoisomerases I and II via frankincense. In vitro studies show that at higher concentrations BA inhibited the proliferation of tumour cells, possibly by inducing programmed cell death (apoptosis) of malignant cells. The frankincense genus from northeast Africa ( BOSWELLIA CARTERII), in particular Eritrea, delivers an outstanding quality of the pharmaceutical drug and is free from heavy metals and preservatives. Based on clinical experience, the treatment of patients having tumor induced oedema derived from brain tumors (astrocytoma, glioblastoma) and brain metastases with frankincense (boscari®), have revealed impressive results. At higher concentrations (3 × 4 - 5 cps.) the effectiveness of anti-oedemic acrtivity and complience is observed. Peri-tumoural brain oedemas are generally suppressed employing glucocorticosteroides that can cause substantial side effects lead to further chronic illnesses especially after long-term application.
In this study the development of aristolochic acid (AA) induced tumors in rats with and without d... more In this study the development of aristolochic acid (AA) induced tumors in rats with and without diallyl sulfide (DAS) was studied. Experiments were also conducted to establish the effects of DAS administration on AA-derived DNA single-stranded regions and DNA adduct formation in the forestomach of such animals. Forestomach, urinary bladder and thymus tumors were induced in male BD-6 rats after oral treatment for 12 weeks with AA (2 x 10 mg/kg/week). Administration of 150 mg/kg DAS intragastrically 4 h prior to AA treatment reduced significantly the number of rats that developed forestomach tumors (6-9 months after the start of experiment). The incidence of AA-induced forestomach tumors was 10% (two out of 20 rats) after co-administration of DAS and 60% (12 out of 20 animals) when AA was administered alone. The high dose of DAS (2 x 150 mg/kg) markedly inhibited the formation of squamous cell carcinomas in the forestomach. However, the thioether did not prevent the formation of forestomach and urinary bladder papillomatosis. Additionally, DAS co-administration decreased the accumulation of single-stranded regions in rat forestomach DNA. Using the nuclease P1 enhancement method of the 32P-postlabeling assay, a decrease in the level of AA-derived adducts was also detected after co-administration of DAS. We conclude that the decrease of DNA damage after DAS co-administration is associated with the delay in conversion of papillomas to malignant forestomach tumors.
Analysis of aristolochic acid I (AAI)-DNA adducts in exfoliated cells in urine, urothelium and en... more Analysis of aristolochic acid I (AAI)-DNA adducts in exfoliated cells in urine, urothelium and entire urinary bladder were studied after oral administration of five daily doses (10 mg/kg body wt) AAI for 3 months to rats. The two major adducts excreted in urine are presumably identical to the two main adducts formed in vitro and in vivo in different organs in the rat, which have previously been characterized in vitro as 7(-deoxyguanosin-N2-yl)-aristolactam I and 7(-deoxyadenosin-N6-yl)-aristolactam I. Urine samples were collected on dry-ice, subsequently pooled and purified according to the protocol of Kadlubar and co-workers. DNA was isolated, digested and AAI-DNA adducts of exfoliated cells in urine and urothelium of rats were detected and quantitated by enhancement methods of the 32P-postlabeling assay, namely nuclease P1 enrichment or butanol extraction. Autoradiograms indicated that adduct patterns in DNA derived from exfoliated cells in urine were very similar to those obtained from DNA isolated from tissues. Quantitative analysis of adducts revealed adduct levels declining for both adducts from DNA isolated from urothelium to DNA isolated from the entire urinary bladder to DNA isolated from exfoliated cells in urine. In general, count rates of two predominant AAI adducts were enhanced by butanol extraction approximately 3- to 8-fold when compared with the nuclease P1 digestion technique. The identity of the two major adducts was confirmed by co-chromatography with eluted spots from in vivo adducts by comparing mobilities on poly-(ethyleneimine)-cellulose plates. Microbiological investigations of the urine revealed no gross contamination with bacteria, so that the isolated DNA supposedly originated from exfoliated urothelial cells. This study indicates that 32P-postlabeling analysis can be used to monitor non-invasively the formation of carcinogen-DNA adducts in animals or humans exposed to carcinogens.
We report the analysis of DNA adducts in the target organ (forestomach) of male Sprague-Dawley ra... more We report the analysis of DNA adducts in the target organ (forestomach) of male Sprague-Dawley rats treated orally with two doses (10 mg/kg body wt) per week for 2 weeks of either aristolochic acid I (AAI), aristolochic acid II (AAII) or the plant extract aristolochic acid (AA). DNA adducts were detected and quantitated using the nuclease P1-enhanced version of the 32P-postlabelling assay. For identification of adducts, reference compounds were prepared by reaction of enzymatically activated AAI and AAII with 3'-purine phosphonucleosides and analysed by the n-butanol enrichment procedure. These reference compounds were assigned to the previously characterized DNA adducts of AAI [7-(deoxyguanosin-N2-yl)-aristolactam I = dG-AAI, 7-(deoxyadenosin-N6-yl)-aristolactam I = dA-AAI] and AAII [7-(deoxyadenosin-N6-yl)-aristolactam II = dA-AAII]. Cross referencing of the carcinogen-modified nucleoside bisphosphates obtained from forestomach DNA with the synthetic standard compounds by ion-exchange chromatography and reversed-phase HPLC demonstrated that the major DNA adducts formed by AAI and AA were identical to dG-AAI and dA-AAI. Likewise, forestomach DNA isolated from AAII-treated rats showed two purine-derived adduct spots, the major one being dA-AAII, the minor one being tentatively identified as 7-(deoxyguanosin-N2-yl)-aristolactam II. A minor adduct detected in forestomach DNA of rats treated with AAI was found to be chromatographically indistinguishable from the adduct identified as dA-AAII, indicating a possible demethoxylation reaction of AAI. Quantitation of DNA adducts revealed that in in vitro reactions with 3'-phosphonucleosides the adduct levels were approximately one order higher for both AAI- and AAII-derived adducts than in forestomach DNA modified with AAI or AAII in vivo. In vitro as well as in vivo adduction by AAI was more efficient than adduction by AAII. The pattern of adduct spots obtained from forestomach DNA of rats treated with the plant extract AA reflected the composition of the extract determined by HPLC analysis. Irrespective of the aristolochic acid used to induce DNA adducts, deoxyadenosine is the major target of modification, pointing to the general importance of deoxyadenosine adducts for chemical carcinogenesis of these naturally occurring products. This study shows that the combination of two independent chromatographic systems considerably enhances the fidelity of identification of DNA adducts with the 32P-postlabelling assay.
Zhonghua bing li xue za zhi Chinese journal of pathology, 1997
To analyse the pathomorphology and the clinical biological behavior of pilomatrix carcinoma as we... more To analyse the pathomorphology and the clinical biological behavior of pilomatrix carcinoma as well as to clarify the main points of diagnosis and differential diagnosis. A review of the clinicopathological, histochemical and immunohistochemical features of 15 pilomatrix carcinomas a well as their clinical data was made. Of the 15 cases, 6 were male and 9 female, aged 11 to 83 (mean age 46 years). The tumor diameter varied from 1.2 cm to 8.0 cm (mean 3.5 cm). Histologically, pilomatrix carcinomas are characterized by sheets and islands of proliferating atypical baseloid cells with an infiltrating border. Transition to squamous, clear and ghost cell are often observed. The trichohyalin granules and tricholin-like acidophilic bodies are seen in tumor cells. Follow-up on 13 cases revealed local recurrence in 7 cases with multiple recurrence in 2 cases. One died of liver metastasis and one died of extensive local spread of the tumor. Pilomatrix carcinoma is a malignant tumor which usual...
Die vorliegende Erfindung betrifft eine wassrige Losung fur die intravenose Infusion enthaltend C... more Die vorliegende Erfindung betrifft eine wassrige Losung fur die intravenose Infusion enthaltend Curcumin und/oder ein oder mehrere Curcuminderivate, Dimethylsulfoxid, einen oder mehrere Losungsvermittler, Natriumselenit, einen Puffer in einem wassrigen Infusionsmedium sowie ein Konzentrat zu ihrer Herstellung enthaltend Curcumin und/oder ein oder mehrere Curcuminderivate, Dimethylsulfoxid, einen oder mehrere Losungsvermittler und Natriumselenit.
The resin of the frankincense tree contains therapeutically effective boswellic acids (BA). Four ... more The resin of the frankincense tree contains therapeutically effective boswellic acids (BA). Four predominant BA selectively inhibit the synthesis of Leucotrienes (LT). A variety of chronic diseases is predominantly accompanied by excessive formation of LT and hence support and prolong the inflammatory process. Starting from the arachidonic acid, the inflammatory and pain cascade is interrupted without significant side effects. Even on the basis of long-term intake, the initial effects are maintained. Indications for a frankincense therapy are all chronic inflammatory and allergic diseases. A different pathway of activity involves the Inhibition of topoisomerases I and II via frankincense. In vitro studies show that at higher concentrations BA inhibited the proliferation of tumour cells, possibly by inducing programmed cell death (apoptosis) of malignant cells. The frankincense genus from northeast Africa ( BOSWELLIA CARTERII), in particular Eritrea, delivers an outstanding quality of the pharmaceutical drug and is free from heavy metals and preservatives. Based on clinical experience, the treatment of patients having tumor induced oedema derived from brain tumors (astrocytoma, glioblastoma) and brain metastases with frankincense (boscari®), have revealed impressive results. At higher concentrations (3 × 4 - 5 cps.) the effectiveness of anti-oedemic acrtivity and complience is observed. Peri-tumoural brain oedemas are generally suppressed employing glucocorticosteroides that can cause substantial side effects lead to further chronic illnesses especially after long-term application.
In this study the development of aristolochic acid (AA) induced tumors in rats with and without d... more In this study the development of aristolochic acid (AA) induced tumors in rats with and without diallyl sulfide (DAS) was studied. Experiments were also conducted to establish the effects of DAS administration on AA-derived DNA single-stranded regions and DNA adduct formation in the forestomach of such animals. Forestomach, urinary bladder and thymus tumors were induced in male BD-6 rats after oral treatment for 12 weeks with AA (2 x 10 mg/kg/week). Administration of 150 mg/kg DAS intragastrically 4 h prior to AA treatment reduced significantly the number of rats that developed forestomach tumors (6-9 months after the start of experiment). The incidence of AA-induced forestomach tumors was 10% (two out of 20 rats) after co-administration of DAS and 60% (12 out of 20 animals) when AA was administered alone. The high dose of DAS (2 x 150 mg/kg) markedly inhibited the formation of squamous cell carcinomas in the forestomach. However, the thioether did not prevent the formation of forestomach and urinary bladder papillomatosis. Additionally, DAS co-administration decreased the accumulation of single-stranded regions in rat forestomach DNA. Using the nuclease P1 enhancement method of the 32P-postlabeling assay, a decrease in the level of AA-derived adducts was also detected after co-administration of DAS. We conclude that the decrease of DNA damage after DAS co-administration is associated with the delay in conversion of papillomas to malignant forestomach tumors.
Analysis of aristolochic acid I (AAI)-DNA adducts in exfoliated cells in urine, urothelium and en... more Analysis of aristolochic acid I (AAI)-DNA adducts in exfoliated cells in urine, urothelium and entire urinary bladder were studied after oral administration of five daily doses (10 mg/kg body wt) AAI for 3 months to rats. The two major adducts excreted in urine are presumably identical to the two main adducts formed in vitro and in vivo in different organs in the rat, which have previously been characterized in vitro as 7(-deoxyguanosin-N2-yl)-aristolactam I and 7(-deoxyadenosin-N6-yl)-aristolactam I. Urine samples were collected on dry-ice, subsequently pooled and purified according to the protocol of Kadlubar and co-workers. DNA was isolated, digested and AAI-DNA adducts of exfoliated cells in urine and urothelium of rats were detected and quantitated by enhancement methods of the 32P-postlabeling assay, namely nuclease P1 enrichment or butanol extraction. Autoradiograms indicated that adduct patterns in DNA derived from exfoliated cells in urine were very similar to those obtained from DNA isolated from tissues. Quantitative analysis of adducts revealed adduct levels declining for both adducts from DNA isolated from urothelium to DNA isolated from the entire urinary bladder to DNA isolated from exfoliated cells in urine. In general, count rates of two predominant AAI adducts were enhanced by butanol extraction approximately 3- to 8-fold when compared with the nuclease P1 digestion technique. The identity of the two major adducts was confirmed by co-chromatography with eluted spots from in vivo adducts by comparing mobilities on poly-(ethyleneimine)-cellulose plates. Microbiological investigations of the urine revealed no gross contamination with bacteria, so that the isolated DNA supposedly originated from exfoliated urothelial cells. This study indicates that 32P-postlabeling analysis can be used to monitor non-invasively the formation of carcinogen-DNA adducts in animals or humans exposed to carcinogens.
We report the analysis of DNA adducts in the target organ (forestomach) of male Sprague-Dawley ra... more We report the analysis of DNA adducts in the target organ (forestomach) of male Sprague-Dawley rats treated orally with two doses (10 mg/kg body wt) per week for 2 weeks of either aristolochic acid I (AAI), aristolochic acid II (AAII) or the plant extract aristolochic acid (AA). DNA adducts were detected and quantitated using the nuclease P1-enhanced version of the 32P-postlabelling assay. For identification of adducts, reference compounds were prepared by reaction of enzymatically activated AAI and AAII with 3'-purine phosphonucleosides and analysed by the n-butanol enrichment procedure. These reference compounds were assigned to the previously characterized DNA adducts of AAI [7-(deoxyguanosin-N2-yl)-aristolactam I = dG-AAI, 7-(deoxyadenosin-N6-yl)-aristolactam I = dA-AAI] and AAII [7-(deoxyadenosin-N6-yl)-aristolactam II = dA-AAII]. Cross referencing of the carcinogen-modified nucleoside bisphosphates obtained from forestomach DNA with the synthetic standard compounds by ion-exchange chromatography and reversed-phase HPLC demonstrated that the major DNA adducts formed by AAI and AA were identical to dG-AAI and dA-AAI. Likewise, forestomach DNA isolated from AAII-treated rats showed two purine-derived adduct spots, the major one being dA-AAII, the minor one being tentatively identified as 7-(deoxyguanosin-N2-yl)-aristolactam II. A minor adduct detected in forestomach DNA of rats treated with AAI was found to be chromatographically indistinguishable from the adduct identified as dA-AAII, indicating a possible demethoxylation reaction of AAI. Quantitation of DNA adducts revealed that in in vitro reactions with 3'-phosphonucleosides the adduct levels were approximately one order higher for both AAI- and AAII-derived adducts than in forestomach DNA modified with AAI or AAII in vivo. In vitro as well as in vivo adduction by AAI was more efficient than adduction by AAII. The pattern of adduct spots obtained from forestomach DNA of rats treated with the plant extract AA reflected the composition of the extract determined by HPLC analysis. Irrespective of the aristolochic acid used to induce DNA adducts, deoxyadenosine is the major target of modification, pointing to the general importance of deoxyadenosine adducts for chemical carcinogenesis of these naturally occurring products. This study shows that the combination of two independent chromatographic systems considerably enhances the fidelity of identification of DNA adducts with the 32P-postlabelling assay.
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