Brazilian journal of biology = Revista brasleira de biologia, 2009
Maytenus ilicifolia and Maytenus aquifolia species are widely used as a cicatrisation agent, an a... more Maytenus ilicifolia and Maytenus aquifolia species are widely used as a cicatrisation agent, an anti-spasmodic, contraceptive, antiulcerogenic, diuretic and analgesic. Considering the importance of these species in popular medicine, this work is focused on the determination of the chemical content of tannins and the triterpenes friedelan-3-one, friedelan-3-ol and friedelin in 15 native populations of Maytenus ilicifolia distributed in the south and mind-west regions of Brazil. Correlation of the concentration of these compounds with the environmental parameters such as average annual temperature, climate, vegetation, geomorphology, latitude and altitude was determined using Pearson's coefficient. Results showed that average annual temperature and climate have significant effect on tannin content at a 95% confidence level. The highest tannin concentration was found in Ponta Porã population, and for the triterpenes investigated, a significant correlation between their concentratio...
... Silvia Egues, Alexandre F. Santos and Andr LD Ramos*. Instituto de Tecnologia e Pesquisa (ITP... more ... Silvia Egues, Alexandre F. Santos and Andr LD Ramos*. Instituto de Tecnologia e Pesquisa (ITP) and Programa de P s-Gradua o em Engenharia de Processos (PEP), Universidade Tiradentes, Av. ... [CrossRef]. 19. Chemat, F.; Poux, M.; Galema, SA J. Chem. Soc., Perkin Trans. ...
This work reports the effects of temperature (20 to 50ºC) and pressure (100 to 250 bar) on the ex... more This work reports the effects of temperature (20 to 50ºC) and pressure (100 to 250 bar) on the extraction yield, chemical characteristics and antimicrobial activity of extracts of Peumus boldus Molina obtained by compressed carbon dioxide extraction. Results showed that the ...
This work investigated the production of fatty acid ethyl esters (FAEEs) from soybean oil using n... more This work investigated the production of fatty acid ethyl esters (FAEEs) from soybean oil using n-hexane as solvent and two commercial lipases as catalysts, Novozym 435 and Lipozyme IM. A Taguchi experimental design was adopted considering the variables temperature (35–65°C), addition of water (0–10 wt/wt%), enzyme (5–20 wt/wt%) concentration, and oil-to-ethanol molar ratio (1:3–1:10). It is shown that complete conversion
The main objective of this work was to investigate the high-pressure phase behavior of the binary... more The main objective of this work was to investigate the high-pressure phase behavior of the binary systems CO2–lemon (Citrus latifolia Tanaka) and CO2–bergamot (Citrus bergamia Risso et Poit.) peel oils. The static synthetic method using a variable-volume view cell was employed for obtaining the experimental data in the CO2 overall composition range of 5–98wt.%, from 313 to 343K and pressures
This work investigated the high-pressure phase behavior of systems containing glycerol, olive oil... more This work investigated the high-pressure phase behavior of systems containing glycerol, olive oil and propane in the presence of surfactant AOT. The static synthetic method, using a variable-volume view cell, was employed for obtaining the experimental data in the temperature range from 298 to 373K, and pressures up to 30MPa. First, the effect of addition of AOT on the vapor
... Fernandes III ; Cláudio Dariva IV ; Sílvia M. Egues IV ; Adailton J. Bortoluzzi V ; Octavio A... more ... Fernandes III ; Cláudio Dariva IV ; Sílvia M. Egues IV ; Adailton J. Bortoluzzi V ; Octavio AC Antunes * , I. ... Keywords: mononuclear copper(II) complex, crystal structure, benzene oxidation. ... Cu(OH 2 ) 2 ]Cl 2 , em metanol, resultou em 1, o qual cristaliza como um cristal irregular azul ...
Polyaniline (PANI) is a water-insoluble polymer that has been used as support for enzyme immobili... more Polyaniline (PANI) is a water-insoluble polymer that has been used as support for enzyme immobilization due to its desirable characteristics, such as ease of preparation, high synthesis yield, high stability to temperature and pH, and resistance to microbial attack. In this work an investigation was carried out to determine the best conditions to immobilize d-hydantoinase (E.C. 3.5.2.2) in this support. As result, a simple and fast methodology for d-hydantoinase immobilization in PANI is described. 100% of proteins were immobilized on the support in concentrations up to 2 mg solid/ml. Higher concentrations led to a lower protein percentage immobilized. After five reaction cycles about a half of d-hydantoinase initial activity was conserved.
CHEMICAL CHARACTERIZATION OF Ocimum basilicum L. EXTRACTS OBTAINED BY HIGH PRESSURE CO2 EXTRACTIO... more CHEMICAL CHARACTERIZATION OF Ocimum basilicum L. EXTRACTS OBTAINED BY HIGH PRESSURE CO2 EXTRACTION. This work reports extraction yield and chemical characterization of the extracts obtained by high-pressure CO2 extraction of a cultivar of ...
The aim of this work is to investigate the production of polypropylene microparticles from polypr... more The aim of this work is to investigate the production of polypropylene microparticles from polypropylene/n-butane solutions using a modified rapid expansion of supercritical solutions (RESS) technique. A commercial polypropylene sample (Polibrasil Resinas S.A.) produced from a Ziegler-Natta catalyst was employed in the experiments. The experiments were performed in a laboratory scale unit where the effects of pre-expansion temperature (373–423K), polymer
Influência das condições de armazenamento sobre o teor de clorofila do brócolis (Brassica olerace... more Influência das condições de armazenamento sobre o teor de clorofila do brócolis (Brassica oleracea) Graziela Paludo1 & Christian O. Reinehr2 1 Acadêmica do Curso de Engenharia de Alimentos UPF, Passo Fundo, RS e-mail: 63225@lci.upf.br 2 Professor do Curso de ...
Effects of conventional heating (CH) and microwave (MW) on the structure and activity of horserad... more Effects of conventional heating (CH) and microwave (MW) on the structure and activity of horseradish peroxidase (HRP) in buffer solution were studied. CH incubation between 30 and 45°C increased activity of HRP, reaching 170% of residual activity (RA) after 4-6h at 45°C. CH treatment at 50 and 60°C caused HRP inactivation: RA was 5.7 and 16.7% after 12h, respectively. Secondary and tertiary HRP structural changes were analyzed by circular dichroism (CD) and intrinsic fluorescence emission, respectively. Under CH, activation of the enzyme was attributed to conformational changes in secondary and tertiary structures. MW treatment had significant effects on the residual activity of HRP. MW treatment at 45°C/30W followed by CH treatment 45°C regenerated the enzyme activity. The greatest loss in activity occurred at 60°C/60W/30min (RA 16.9%); without recovery of the original activity. The inactivation of MW-treated HRP was related to the loss of tertiary structure, indicating changes around the tryptophan environment.
Brazilian journal of biology = Revista brasleira de biologia, 2009
Maytenus ilicifolia and Maytenus aquifolia species are widely used as a cicatrisation agent, an a... more Maytenus ilicifolia and Maytenus aquifolia species are widely used as a cicatrisation agent, an anti-spasmodic, contraceptive, antiulcerogenic, diuretic and analgesic. Considering the importance of these species in popular medicine, this work is focused on the determination of the chemical content of tannins and the triterpenes friedelan-3-one, friedelan-3-ol and friedelin in 15 native populations of Maytenus ilicifolia distributed in the south and mind-west regions of Brazil. Correlation of the concentration of these compounds with the environmental parameters such as average annual temperature, climate, vegetation, geomorphology, latitude and altitude was determined using Pearson's coefficient. Results showed that average annual temperature and climate have significant effect on tannin content at a 95% confidence level. The highest tannin concentration was found in Ponta Porã population, and for the triterpenes investigated, a significant correlation between their concentratio...
... Silvia Egues, Alexandre F. Santos and Andr LD Ramos*. Instituto de Tecnologia e Pesquisa (ITP... more ... Silvia Egues, Alexandre F. Santos and Andr LD Ramos*. Instituto de Tecnologia e Pesquisa (ITP) and Programa de P s-Gradua o em Engenharia de Processos (PEP), Universidade Tiradentes, Av. ... [CrossRef]. 19. Chemat, F.; Poux, M.; Galema, SA J. Chem. Soc., Perkin Trans. ...
This work reports the effects of temperature (20 to 50ºC) and pressure (100 to 250 bar) on the ex... more This work reports the effects of temperature (20 to 50ºC) and pressure (100 to 250 bar) on the extraction yield, chemical characteristics and antimicrobial activity of extracts of Peumus boldus Molina obtained by compressed carbon dioxide extraction. Results showed that the ...
This work investigated the production of fatty acid ethyl esters (FAEEs) from soybean oil using n... more This work investigated the production of fatty acid ethyl esters (FAEEs) from soybean oil using n-hexane as solvent and two commercial lipases as catalysts, Novozym 435 and Lipozyme IM. A Taguchi experimental design was adopted considering the variables temperature (35–65°C), addition of water (0–10 wt/wt%), enzyme (5–20 wt/wt%) concentration, and oil-to-ethanol molar ratio (1:3–1:10). It is shown that complete conversion
The main objective of this work was to investigate the high-pressure phase behavior of the binary... more The main objective of this work was to investigate the high-pressure phase behavior of the binary systems CO2–lemon (Citrus latifolia Tanaka) and CO2–bergamot (Citrus bergamia Risso et Poit.) peel oils. The static synthetic method using a variable-volume view cell was employed for obtaining the experimental data in the CO2 overall composition range of 5–98wt.%, from 313 to 343K and pressures
This work investigated the high-pressure phase behavior of systems containing glycerol, olive oil... more This work investigated the high-pressure phase behavior of systems containing glycerol, olive oil and propane in the presence of surfactant AOT. The static synthetic method, using a variable-volume view cell, was employed for obtaining the experimental data in the temperature range from 298 to 373K, and pressures up to 30MPa. First, the effect of addition of AOT on the vapor
... Fernandes III ; Cláudio Dariva IV ; Sílvia M. Egues IV ; Adailton J. Bortoluzzi V ; Octavio A... more ... Fernandes III ; Cláudio Dariva IV ; Sílvia M. Egues IV ; Adailton J. Bortoluzzi V ; Octavio AC Antunes * , I. ... Keywords: mononuclear copper(II) complex, crystal structure, benzene oxidation. ... Cu(OH 2 ) 2 ]Cl 2 , em metanol, resultou em 1, o qual cristaliza como um cristal irregular azul ...
Polyaniline (PANI) is a water-insoluble polymer that has been used as support for enzyme immobili... more Polyaniline (PANI) is a water-insoluble polymer that has been used as support for enzyme immobilization due to its desirable characteristics, such as ease of preparation, high synthesis yield, high stability to temperature and pH, and resistance to microbial attack. In this work an investigation was carried out to determine the best conditions to immobilize d-hydantoinase (E.C. 3.5.2.2) in this support. As result, a simple and fast methodology for d-hydantoinase immobilization in PANI is described. 100% of proteins were immobilized on the support in concentrations up to 2 mg solid/ml. Higher concentrations led to a lower protein percentage immobilized. After five reaction cycles about a half of d-hydantoinase initial activity was conserved.
CHEMICAL CHARACTERIZATION OF Ocimum basilicum L. EXTRACTS OBTAINED BY HIGH PRESSURE CO2 EXTRACTIO... more CHEMICAL CHARACTERIZATION OF Ocimum basilicum L. EXTRACTS OBTAINED BY HIGH PRESSURE CO2 EXTRACTION. This work reports extraction yield and chemical characterization of the extracts obtained by high-pressure CO2 extraction of a cultivar of ...
The aim of this work is to investigate the production of polypropylene microparticles from polypr... more The aim of this work is to investigate the production of polypropylene microparticles from polypropylene/n-butane solutions using a modified rapid expansion of supercritical solutions (RESS) technique. A commercial polypropylene sample (Polibrasil Resinas S.A.) produced from a Ziegler-Natta catalyst was employed in the experiments. The experiments were performed in a laboratory scale unit where the effects of pre-expansion temperature (373–423K), polymer
Influência das condições de armazenamento sobre o teor de clorofila do brócolis (Brassica olerace... more Influência das condições de armazenamento sobre o teor de clorofila do brócolis (Brassica oleracea) Graziela Paludo1 & Christian O. Reinehr2 1 Acadêmica do Curso de Engenharia de Alimentos UPF, Passo Fundo, RS e-mail: 63225@lci.upf.br 2 Professor do Curso de ...
Effects of conventional heating (CH) and microwave (MW) on the structure and activity of horserad... more Effects of conventional heating (CH) and microwave (MW) on the structure and activity of horseradish peroxidase (HRP) in buffer solution were studied. CH incubation between 30 and 45°C increased activity of HRP, reaching 170% of residual activity (RA) after 4-6h at 45°C. CH treatment at 50 and 60°C caused HRP inactivation: RA was 5.7 and 16.7% after 12h, respectively. Secondary and tertiary HRP structural changes were analyzed by circular dichroism (CD) and intrinsic fluorescence emission, respectively. Under CH, activation of the enzyme was attributed to conformational changes in secondary and tertiary structures. MW treatment had significant effects on the residual activity of HRP. MW treatment at 45°C/30W followed by CH treatment 45°C regenerated the enzyme activity. The greatest loss in activity occurred at 60°C/60W/30min (RA 16.9%); without recovery of the original activity. The inactivation of MW-treated HRP was related to the loss of tertiary structure, indicating changes around the tryptophan environment.
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