Objective To explore how denture-related experiences affect older adults' quality of life... more Objective To explore how denture-related experiences affect older adults' quality of life using a qualitative assessment of the Oral Health Impact Profile for Edentulous individuals (OHIP-Edent). Materials and methods Twenty elderly individuals were interviewed before and 3 months after delivering new complete dentures, using an open-ended interview guide based on the OHIP-Edent. Interviews were audio-recorded and transcribed. Data were open coded and thematically analyzed following a Grounded Theory approach. Findings were integrated and constantly compared to understand the interviewees' difficulties, beliefs, and perceptions. Results Three interconnected themes were developed: functional and psychosocial impairments, and coping strategies. Even when posed as an open-ended format, the wording of some OHIP-Edent items was confusing while others were not relevant to the respondents. New categories related to speaking, smiling, swallowing, emotional and functional coping emerged from the interviews. Interviewees adapted to chewing and swallowing difficulties through food avoidance, modification of food choice and preparation techniques, and changes in dietary behaviors. Conclusions Denture wearing is a daily challenging experience that encompasses various functional and psychosocial aspects and sheds light on the need for addressing the coping strategies employed by patients, as the current OHIP-Edent items may not fully represent other deemed important aspects of the quality of life of individuals who wear dentures. Clinical relevance Dentists must not solely rely on structured questionnaires to explore the impact of denture wearing and treatment outcomes. Clinicians can use a more holistic approach to comprehend older adults' experiences with dentures including advice about coping mechanisms, food preparation techniques, and meal planning.
A aplicação de concepções éticas, humanistas e cuidadoras no ensino e no exercício profissional n... more A aplicação de concepções éticas, humanistas e cuidadoras no ensino e no exercício profissional no Sistema Único de Saúde (SUS), prerrogativa das Diretrizes Curriculares Nacionais (DCN) estabelecidas há mais de uma década, ainda é um desafio nos currículos dos cursos da graduação em saúde do Brasil. Este estudo foi desenvolvido pelo grupo PET-Saúde (Programa de Educação pelo Trabalho para a Saúde) GraduaSUS e teve o objetivo de utilizar os conteúdos dos planos de ensino das atividades curriculares obrigatórias para analisar os currículos dos cursos de graduação em Medicina, Odontologia, Nutrição, Enfermagem, Psicologia e Fisioterapia da Universidade Federal do Rio Grande do Sul (UFRGS). A análise documental centrou-se na busca de termos que revelassem conteúdos e competências abordados nas atividades de ensino curriculares obrigatórias e que indicassem uma formação que contemplasse o SUS. Foi realizada análise documental de 325 planos de ensino de disciplinas e de estágios obrigatór...
Background: Recent findings indicated that bruxism may be involved in esophageal acidification an... more Background: Recent findings indicated that bruxism may be involved in esophageal acidification and acid clearance. Objective: This study aimed to evaluate the association between bruxism and reported symptom of heartburn. Methods: A total of 68 volunteers of the Gastroenterology Service at the Clinical Hospital of Porto Alegre/Brazil, were recruited and divided into two groups: absence (A, n=26) or presence of heartburn (B, n=42). Subjects with malocclusion, less than 24 teeth, morbid obesity and incomplete questionnaires were excluded. Group A were accompanying persons who did not report: heartburn, gastroenterological disease and use of heartburn medications. Group B was composed by subjects who reported: heartburn with or without the use of heartburn medication. Hearburn was assessed by a questionnaire of signs and symptoms. Bruxism was diagnosed under blind condition by evaluation of tooth wear, muscle discomfort and patient self-report, according to the American Academy of Slee...
A aplicacao de concepcoes eticas, humanistas e cuidadoras no ensino e no exercicio profissional n... more A aplicacao de concepcoes eticas, humanistas e cuidadoras no ensino e no exercicio profissional no Sistema Unico de Saude (SUS), prerrogativa das Diretrizes Curriculares Nacionais (DCN) estabelecidas ha mais de uma decada, ainda e um desafio nos curriculos dos cursos da graduacao em saude do Brasil. Este estudo foi desenvolvido pelo grupo PET-Saude (Programa de Educacao pelo Trabalho para a Saude) GraduaSUS e teve o objetivo de utilizar os conteudos dos planos de ensino das atividades curriculares obrigatorias para analisar os curriculos dos cursos de graduacao em Medicina, Odontologia, Nutricao, Enfermagem, Psicologia e Fisioterapia da Universidade Federal do Rio Grande do Sul (UFRGS). A analise documental centrou-se na busca de termos que revelassem conteudos e competencias abordados nas atividades de ensino curriculares obrigatorias e que indicassem uma formacao que contemplasse o SUS. Foi realizada analise documental de 325 planos de ensino de disciplinas e de estagios obrigator...
STATEMENT OF PROBLEM A vinegar-hydrogen peroxide mixture has been reported to be effective in eli... more STATEMENT OF PROBLEM A vinegar-hydrogen peroxide mixture has been reported to be effective in eliminating Candida albicans and Staphylococcus aureus from acrylic resin, and its action has been reported to be comparable with that of sodium hypochlorite or peracetic acid. However, the effects of this mixture on cobalt-chromium alloys remain unknown. PURPOSE The purpose of this in vitro study was to evaluate the surface roughness, Knoop microhardness, surface free energy, and wettability of a cobalt-chromium alloy when exposed to a vinegar-hydrogen peroxide mixture. MATERIAL AND METHODS Fifty specimens of cobalt-chromium alloy were fabricated and immersed for 900 minutes, simulating 3 months of a daily 10-minute immersion in the following chemical agents (n=10): distilled water (W); 0.5% sodium hypochlorite (H); 3% hydrogen peroxide and water dilution in 1:1 ratio (HP); white-wine vinegar and water dilution in 1:1 ratio (V); and vinegar and hydrogen peroxide mixture in 1:1 ratio (VHP)....
A deficiencia de vitamina D pode representar um fator de risco para a perda de implantes. Os obje... more A deficiencia de vitamina D pode representar um fator de risco para a perda de implantes. Os objetivos deste estudo foram: (a) investigar a influencia da deficiencia de vitamina D nos padroes de expressao genica durante a osseointegracao in vivo, e (b) avaliar a influencia da vitamina D e do titânio na expressao de genes relacionados ao metabolismo osseo in vitro. Para atingir o primeiro objetivo, dezesseis ratos foram distribuidos em quatro grupos segundo o tipo de dieta recebida e a insercao ou nao de implante em seus femures: OSTV+ (dieta padrao/ osteotomia), OSTV- (dieta deficiente de vitamina D/ osteotomia), NTV+ (dieta padrao/ insercao de implante) e NTV- (dieta deficiente de vitamina D/ insercao de implante). Duas semanas apos a cirurgia, o RNA foi isolado a partir do tecido osseo, e as expressoes de aproximadamente 41.000 transcritos foram determinadas pela tecnologia de microarray. A comparacao entre os grupos foi realizada atraves de ANOVA bifatorial (correcao de Benjamini...
Peracetic acid (PAA) has been used to sterilize biomaterial scaffolds and allografts before their... more Peracetic acid (PAA) has been used to sterilize biomaterial scaffolds and allografts before their implantation. Although the antimicrobial effectiveness of PAA is widely known, there are no studies investigating its cytotoxicity on keratinocytes. This study aimed to investigate the cytotoxicity of PAA concentrations on keratinocytes by growing HaCaT cells in culture medium. Different concentrations of PAA (control-untreated, 0.01, 0.1, 1, 10, 100, 200, 400, 800, 1200, 1600, and 2000 ppm) were added to the culture wells and allowed to be in direct contact with cells for up to 24 hours. Cytotoxicity was quantitatively and qualitatively determined by cell viability assay and analysis of morphological changes. Statistical analysis was performed with 1-way analysis of variance and Tukey test at 5% significance. Cells treated with 0.01 and 0.1 ppm followed the same morphological pattern of untreated cells, whereas cells treated with 1.0 ppm presented about 20% of floating cells and dark cytoplasmic granules. More than 50% of the cells treated with 10 and 100 ppm were destroyed, whereas the attached ones showed unclear and interrupted cell membranes. Concentrations of 1 ppm or greater had less than 64.4% of viable cells compared with the control group. This study concluded that exposure of keratinocytes to concentrations of 1 ppm or greater of PAA resulted in strong cytotoxic effects.
STATEMENT OF PROBLEM Soaking dentures in vinegar or hydrogen peroxide does not seem to remove the... more STATEMENT OF PROBLEM Soaking dentures in vinegar or hydrogen peroxide does not seem to remove the microorganisms involved with prosthetic stomatitis efficiently. A mixture of these 2 substances may be effective, but studies are lacking. PURPOSE The purpose of this in vitro study was to evaluate the antimicrobial effect and cytotoxic activity of vinegar-hydrogen peroxide mixtures against Candida albicans and Staphylococcus aureus. MATERIAL AND METHODS For antimicrobial tests, planktonic cells and biofilms of C. albicans and S. aureus cultured on acrylic resin disks were exposed to 0.5% sodium hypochlorite; 0.2% peracetic acid; vinegar-hydrogen peroxide mixtures at concentration ratios 1:1, 1:3, and 3:1; vinegar-water mixtures at concentration ratios 1:1, 1:3, and 3:1; and hydrogen peroxide-water mixtures at concentration ratios 1:1, 1:3, and 3:1. Antimicrobial activity was evaluated by counting viable colony-forming units after disinfection. For cytotoxicity tests, the 1:1 vinegar-hydrogen peroxide mixture was serially diluted (10-1 to 10-4) and allowed to be in direct contact with HaCaT keratinocytes for 24 hours. Cytotoxicity was quantitatively and qualitatively determined by counting the number of viable cells and analyzing morphological cell changes. RESULTS All vinegar-hydrogen peroxide mixtures, sodium hypochlorite, and peracetic acid efficiently eliminated C. albicans and S. aureus (P<.05), whereas vinegar and hydrogen peroxide solutions used separately were not as efficient as the experimental mixtures. The 10-3 and 10-4 dilutions of vinegar-hydrogen peroxide solutions were considered noncytotoxic, whereas dilutions below 10-2 were strongly cytotoxic, comparable with the 10-2 dilution of 0.2% peracetic acid. CONCLUSIONS The vinegar-hydrogen peroxide mixture effectively eliminated C. albicans and S. aureus from acrylic resin. Dilutions equal or below 10-2 of this mixture presented strong cytotoxic effects.
Objective To explore how denture-related experiences affect older adults&#39; quality of life... more Objective To explore how denture-related experiences affect older adults&#39; quality of life using a qualitative assessment of the Oral Health Impact Profile for Edentulous individuals (OHIP-Edent). Materials and methods Twenty elderly individuals were interviewed before and 3 months after delivering new complete dentures, using an open-ended interview guide based on the OHIP-Edent. Interviews were audio-recorded and transcribed. Data were open coded and thematically analyzed following a Grounded Theory approach. Findings were integrated and constantly compared to understand the interviewees&#39; difficulties, beliefs, and perceptions. Results Three interconnected themes were developed: functional and psychosocial impairments, and coping strategies. Even when posed as an open-ended format, the wording of some OHIP-Edent items was confusing while others were not relevant to the respondents. New categories related to speaking, smiling, swallowing, emotional and functional coping emerged from the interviews. Interviewees adapted to chewing and swallowing difficulties through food avoidance, modification of food choice and preparation techniques, and changes in dietary behaviors. Conclusions Denture wearing is a daily challenging experience that encompasses various functional and psychosocial aspects and sheds light on the need for addressing the coping strategies employed by patients, as the current OHIP-Edent items may not fully represent other deemed important aspects of the quality of life of individuals who wear dentures. Clinical relevance Dentists must not solely rely on structured questionnaires to explore the impact of denture wearing and treatment outcomes. Clinicians can use a more holistic approach to comprehend older adults&#39; experiences with dentures including advice about coping mechanisms, food preparation techniques, and meal planning.
A aplicação de concepções éticas, humanistas e cuidadoras no ensino e no exercício profissional n... more A aplicação de concepções éticas, humanistas e cuidadoras no ensino e no exercício profissional no Sistema Único de Saúde (SUS), prerrogativa das Diretrizes Curriculares Nacionais (DCN) estabelecidas há mais de uma década, ainda é um desafio nos currículos dos cursos da graduação em saúde do Brasil. Este estudo foi desenvolvido pelo grupo PET-Saúde (Programa de Educação pelo Trabalho para a Saúde) GraduaSUS e teve o objetivo de utilizar os conteúdos dos planos de ensino das atividades curriculares obrigatórias para analisar os currículos dos cursos de graduação em Medicina, Odontologia, Nutrição, Enfermagem, Psicologia e Fisioterapia da Universidade Federal do Rio Grande do Sul (UFRGS). A análise documental centrou-se na busca de termos que revelassem conteúdos e competências abordados nas atividades de ensino curriculares obrigatórias e que indicassem uma formação que contemplasse o SUS. Foi realizada análise documental de 325 planos de ensino de disciplinas e de estágios obrigatór...
Background: Recent findings indicated that bruxism may be involved in esophageal acidification an... more Background: Recent findings indicated that bruxism may be involved in esophageal acidification and acid clearance. Objective: This study aimed to evaluate the association between bruxism and reported symptom of heartburn. Methods: A total of 68 volunteers of the Gastroenterology Service at the Clinical Hospital of Porto Alegre/Brazil, were recruited and divided into two groups: absence (A, n=26) or presence of heartburn (B, n=42). Subjects with malocclusion, less than 24 teeth, morbid obesity and incomplete questionnaires were excluded. Group A were accompanying persons who did not report: heartburn, gastroenterological disease and use of heartburn medications. Group B was composed by subjects who reported: heartburn with or without the use of heartburn medication. Hearburn was assessed by a questionnaire of signs and symptoms. Bruxism was diagnosed under blind condition by evaluation of tooth wear, muscle discomfort and patient self-report, according to the American Academy of Slee...
A aplicacao de concepcoes eticas, humanistas e cuidadoras no ensino e no exercicio profissional n... more A aplicacao de concepcoes eticas, humanistas e cuidadoras no ensino e no exercicio profissional no Sistema Unico de Saude (SUS), prerrogativa das Diretrizes Curriculares Nacionais (DCN) estabelecidas ha mais de uma decada, ainda e um desafio nos curriculos dos cursos da graduacao em saude do Brasil. Este estudo foi desenvolvido pelo grupo PET-Saude (Programa de Educacao pelo Trabalho para a Saude) GraduaSUS e teve o objetivo de utilizar os conteudos dos planos de ensino das atividades curriculares obrigatorias para analisar os curriculos dos cursos de graduacao em Medicina, Odontologia, Nutricao, Enfermagem, Psicologia e Fisioterapia da Universidade Federal do Rio Grande do Sul (UFRGS). A analise documental centrou-se na busca de termos que revelassem conteudos e competencias abordados nas atividades de ensino curriculares obrigatorias e que indicassem uma formacao que contemplasse o SUS. Foi realizada analise documental de 325 planos de ensino de disciplinas e de estagios obrigator...
STATEMENT OF PROBLEM A vinegar-hydrogen peroxide mixture has been reported to be effective in eli... more STATEMENT OF PROBLEM A vinegar-hydrogen peroxide mixture has been reported to be effective in eliminating Candida albicans and Staphylococcus aureus from acrylic resin, and its action has been reported to be comparable with that of sodium hypochlorite or peracetic acid. However, the effects of this mixture on cobalt-chromium alloys remain unknown. PURPOSE The purpose of this in vitro study was to evaluate the surface roughness, Knoop microhardness, surface free energy, and wettability of a cobalt-chromium alloy when exposed to a vinegar-hydrogen peroxide mixture. MATERIAL AND METHODS Fifty specimens of cobalt-chromium alloy were fabricated and immersed for 900 minutes, simulating 3 months of a daily 10-minute immersion in the following chemical agents (n=10): distilled water (W); 0.5% sodium hypochlorite (H); 3% hydrogen peroxide and water dilution in 1:1 ratio (HP); white-wine vinegar and water dilution in 1:1 ratio (V); and vinegar and hydrogen peroxide mixture in 1:1 ratio (VHP)....
A deficiencia de vitamina D pode representar um fator de risco para a perda de implantes. Os obje... more A deficiencia de vitamina D pode representar um fator de risco para a perda de implantes. Os objetivos deste estudo foram: (a) investigar a influencia da deficiencia de vitamina D nos padroes de expressao genica durante a osseointegracao in vivo, e (b) avaliar a influencia da vitamina D e do titânio na expressao de genes relacionados ao metabolismo osseo in vitro. Para atingir o primeiro objetivo, dezesseis ratos foram distribuidos em quatro grupos segundo o tipo de dieta recebida e a insercao ou nao de implante em seus femures: OSTV+ (dieta padrao/ osteotomia), OSTV- (dieta deficiente de vitamina D/ osteotomia), NTV+ (dieta padrao/ insercao de implante) e NTV- (dieta deficiente de vitamina D/ insercao de implante). Duas semanas apos a cirurgia, o RNA foi isolado a partir do tecido osseo, e as expressoes de aproximadamente 41.000 transcritos foram determinadas pela tecnologia de microarray. A comparacao entre os grupos foi realizada atraves de ANOVA bifatorial (correcao de Benjamini...
Peracetic acid (PAA) has been used to sterilize biomaterial scaffolds and allografts before their... more Peracetic acid (PAA) has been used to sterilize biomaterial scaffolds and allografts before their implantation. Although the antimicrobial effectiveness of PAA is widely known, there are no studies investigating its cytotoxicity on keratinocytes. This study aimed to investigate the cytotoxicity of PAA concentrations on keratinocytes by growing HaCaT cells in culture medium. Different concentrations of PAA (control-untreated, 0.01, 0.1, 1, 10, 100, 200, 400, 800, 1200, 1600, and 2000 ppm) were added to the culture wells and allowed to be in direct contact with cells for up to 24 hours. Cytotoxicity was quantitatively and qualitatively determined by cell viability assay and analysis of morphological changes. Statistical analysis was performed with 1-way analysis of variance and Tukey test at 5% significance. Cells treated with 0.01 and 0.1 ppm followed the same morphological pattern of untreated cells, whereas cells treated with 1.0 ppm presented about 20% of floating cells and dark cytoplasmic granules. More than 50% of the cells treated with 10 and 100 ppm were destroyed, whereas the attached ones showed unclear and interrupted cell membranes. Concentrations of 1 ppm or greater had less than 64.4% of viable cells compared with the control group. This study concluded that exposure of keratinocytes to concentrations of 1 ppm or greater of PAA resulted in strong cytotoxic effects.
STATEMENT OF PROBLEM Soaking dentures in vinegar or hydrogen peroxide does not seem to remove the... more STATEMENT OF PROBLEM Soaking dentures in vinegar or hydrogen peroxide does not seem to remove the microorganisms involved with prosthetic stomatitis efficiently. A mixture of these 2 substances may be effective, but studies are lacking. PURPOSE The purpose of this in vitro study was to evaluate the antimicrobial effect and cytotoxic activity of vinegar-hydrogen peroxide mixtures against Candida albicans and Staphylococcus aureus. MATERIAL AND METHODS For antimicrobial tests, planktonic cells and biofilms of C. albicans and S. aureus cultured on acrylic resin disks were exposed to 0.5% sodium hypochlorite; 0.2% peracetic acid; vinegar-hydrogen peroxide mixtures at concentration ratios 1:1, 1:3, and 3:1; vinegar-water mixtures at concentration ratios 1:1, 1:3, and 3:1; and hydrogen peroxide-water mixtures at concentration ratios 1:1, 1:3, and 3:1. Antimicrobial activity was evaluated by counting viable colony-forming units after disinfection. For cytotoxicity tests, the 1:1 vinegar-hydrogen peroxide mixture was serially diluted (10-1 to 10-4) and allowed to be in direct contact with HaCaT keratinocytes for 24 hours. Cytotoxicity was quantitatively and qualitatively determined by counting the number of viable cells and analyzing morphological cell changes. RESULTS All vinegar-hydrogen peroxide mixtures, sodium hypochlorite, and peracetic acid efficiently eliminated C. albicans and S. aureus (P<.05), whereas vinegar and hydrogen peroxide solutions used separately were not as efficient as the experimental mixtures. The 10-3 and 10-4 dilutions of vinegar-hydrogen peroxide solutions were considered noncytotoxic, whereas dilutions below 10-2 were strongly cytotoxic, comparable with the 10-2 dilution of 0.2% peracetic acid. CONCLUSIONS The vinegar-hydrogen peroxide mixture effectively eliminated C. albicans and S. aureus from acrylic resin. Dilutions equal or below 10-2 of this mixture presented strong cytotoxic effects.
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