Messenger RNA (mRNA) sequences were amplified from whole blood prepared in guanidine thiocyanate ... more Messenger RNA (mRNA) sequences were amplified from whole blood prepared in guanidine thiocyanate (GuSCN) after capture of the mRNA with an affinity membrane.
HERV-K is a 50-copy, human endogenous, class 1 retroviral element that contains some polycistrons... more HERV-K is a 50-copy, human endogenous, class 1 retroviral element that contains some polycistrons with gag, pol, and env open reading frames. Although expression of HERV-K proviruses has been shown in cultured human cell lines, expression of these elements has not been shown in human blood leukocytes. Using both reverse transcriptase-polymerase chain reaction and ribonuclease protection techniques, we show HERV-K pol gene expression in human blood leukocytes. Expression in blood leukocytes from 7 normal individuals was from a variety of different HERV-K proviruses, while restricted expression was observed in blood cells of 5 leukemia patients and 3 polycythemia vera patients. Evidence is presented suggesting that the restricted expression in leukemia blood cells is a result of gene regulation, not gene amplification.
Gene expression at the mRNA level is likely to be a rapidly responding intermediate biomarker for... more Gene expression at the mRNA level is likely to be a rapidly responding intermediate biomarker for use as a surrogate endpoint in Phase II clinical trials of breast cancer. Using a technology called capture-RT-PCR, we have been able to show ectopic int-2 mRNA ...
ABSTRACT Commercially available packed microbore columns have been used to demonstrate the potent... more ABSTRACT Commercially available packed microbore columns have been used to demonstrate the potential of microbore high performance liquid chromatography (micro-LC) systems. The necessity of optimizing the chromatographic system for micro-LC is demonstrated using sulfa drugs and antibiotic standards, and some of the advantages of micro-LC such as high mass sensitivity are shown. Finally micro-LC has been used for the determination of two drugs, reserpine and trichlormethiazide in biological fluids.
ABSTRACT Commercially available packed microbore columns have been used to demonstrate the potent... more ABSTRACT Commercially available packed microbore columns have been used to demonstrate the potential of microbore high performance liquid chromatography (micro-LC) systems. The necessity of optimizing the chromatographic system for micro-LC is demonstrated using sulfa drugs and antibiotic standards, and some of the advantages of micro-LC such as high mass sensitivity are shown. Finally micro-LC has been used for the determination of two drugs, reserpine and trichlormethiazide in biological fluids.
Journal of Chromatography B Biomedical Sciences and Applications, Dec 31, 1985
Micro-liquid chromatography-mass spectrometry (micro-LC-MS) was utilized to quantitatively determ... more Micro-liquid chromatography-mass spectrometry (micro-LC-MS) was utilized to quantitatively determine betamethasone and its major unconjugated metabolite, 6 beta-hydroxybetamethasone, in equine plasma and urine. The advantage of micro-LC-MS over conventional gas chromatography-mass spectrometry in corticosteroid determination is illustrated and the reliable, steadfast nature of micro-LC-MS is demonstrated through example.
Messenger RNA (mRNA) sequences were amplified from whole blood prepared in guanidine thiocyanate ... more Messenger RNA (mRNA) sequences were amplified from whole blood prepared in guanidine thiocyanate (GuSCN) after capture of the mRNA with an affinity membrane.
HERV-K is a 50-copy, human endogenous, class 1 retroviral element that contains some polycistrons... more HERV-K is a 50-copy, human endogenous, class 1 retroviral element that contains some polycistrons with gag, pol, and env open reading frames. Although expression of HERV-K proviruses has been shown in cultured human cell lines, expression of these elements has not been shown in human blood leukocytes. Using both reverse transcriptase-polymerase chain reaction and ribonuclease protection techniques, we show HERV-K pol gene expression in human blood leukocytes. Expression in blood leukocytes from 7 normal individuals was from a variety of different HERV-K proviruses, while restricted expression was observed in blood cells of 5 leukemia patients and 3 polycythemia vera patients. Evidence is presented suggesting that the restricted expression in leukemia blood cells is a result of gene regulation, not gene amplification.
Gene expression at the mRNA level is likely to be a rapidly responding intermediate biomarker for... more Gene expression at the mRNA level is likely to be a rapidly responding intermediate biomarker for use as a surrogate endpoint in Phase II clinical trials of breast cancer. Using a technology called capture-RT-PCR, we have been able to show ectopic int-2 mRNA ...
ABSTRACT Commercially available packed microbore columns have been used to demonstrate the potent... more ABSTRACT Commercially available packed microbore columns have been used to demonstrate the potential of microbore high performance liquid chromatography (micro-LC) systems. The necessity of optimizing the chromatographic system for micro-LC is demonstrated using sulfa drugs and antibiotic standards, and some of the advantages of micro-LC such as high mass sensitivity are shown. Finally micro-LC has been used for the determination of two drugs, reserpine and trichlormethiazide in biological fluids.
ABSTRACT Commercially available packed microbore columns have been used to demonstrate the potent... more ABSTRACT Commercially available packed microbore columns have been used to demonstrate the potential of microbore high performance liquid chromatography (micro-LC) systems. The necessity of optimizing the chromatographic system for micro-LC is demonstrated using sulfa drugs and antibiotic standards, and some of the advantages of micro-LC such as high mass sensitivity are shown. Finally micro-LC has been used for the determination of two drugs, reserpine and trichlormethiazide in biological fluids.
Journal of Chromatography B Biomedical Sciences and Applications, Dec 31, 1985
Micro-liquid chromatography-mass spectrometry (micro-LC-MS) was utilized to quantitatively determ... more Micro-liquid chromatography-mass spectrometry (micro-LC-MS) was utilized to quantitatively determine betamethasone and its major unconjugated metabolite, 6 beta-hydroxybetamethasone, in equine plasma and urine. The advantage of micro-LC-MS over conventional gas chromatography-mass spectrometry in corticosteroid determination is illustrated and the reliable, steadfast nature of micro-LC-MS is demonstrated through example.
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Papers by Kevin Cuddy