Dr. Dalia Abd Allah Youssef. Pests and plant protection Division. Agricultural and Biological Department. National research center. Teaching in the High Institute of Agricultural Co-Operation- Egypt.
International Journal of Natural Resource Ecology and Management, 2019
Abstract: Nanotechnology is considered to be a development way to gain efficient and potential ap... more Abstract: Nanotechnology is considered to be a development way to gain efficient and potential approaches towards pest control. The effect of both neem and peppermint oils as bulk, nano and loaded nano-emulsions have been studied against the different biological aspects of the 2nd larval instar of Agrotis ipsilon (Hufn.) under laboratory conditions. Their effects on enzymatic activities were investigated for the 2nd and 4th larval instars. The effects of adding the different formulations of either neem or peppermint oils to artificial diet of 2nd instar larvae show significant elongation of the larval duration; percentage mortalities were increased as well as larval malformations. It was recorded that nano-formulations (emulsion or loaded) significantly decreased the pupal weight and significantly increased the pupal duration, percentage pupal mortalities and pupal malformation. Adult longevity show insignificant effect, while female fecundity and percentage egg fertility show significant decrease in comparison with the control. Results of enzymatic activities show marked effects of the three formulations of either neem or peppermint oil. Significant inhibitions were recorded for amylase, invertase, trehalase, protease and alkaline phosphatase. However, significant increase in the activities of cuticle phenoloxidase and chitinase were recorded. Marked variations were recorded between nano-formulations and the bulk form.
Applications of nanotechnology in agriculture will result in the development of efficient and pot... more Applications of nanotechnology in agriculture will result in the development of efficient and potential approaches towards the management of insect pests. The toxicity effects of four essential oils peppermint, thyme, camphor and sage oils were tested against the fourth instar larvae of Agrotis ipsilon to select the most effective essential oil to be converted to the nano form. According to the results obtained, peppermint oil was the most toxic compound, which has been used in the present investigation compared with neem oil. The toxicity of bulk and nano-formulations of neem and pepper mint oils were tested against 2nd and 4th instar larvae of A. ipsilon under laboratory conditions of 25±2 °C& 65-70 % R.H.rel-ative humidity The results show that the LC50 value (the concentration used which kill 50% of the tested individuals)of loaded neem or pepper mint were lower (0.62 and 36.47 ppm) compared with neem or pepper mint oil nano-emulsion and bulk neem for the second larval instar. The different formulations of neem are more potent than in case of peppermint oil, as LC50 and LC90 values were significantly lower. The same trend was found concerning the 4th larval instar. Age of treated larvae had a detrimental effect on the response to the compounds tested. It was noticed that the younger larvae were much more sensitive to the prepared compounds compared to the older ones. The least LC50 value for loaded neem nano-emulsion was 6.68 ppm compared with the highest value for bulk neem oil (16.68 ppm). Also, LC90 values followed the same trend as in case ofLC50. Again, the toxicity of loaded peppermint oil had the most insecticidal activity as expressed by the lowest LC50 value (51.9 ppm) with more insecticidal effect than the bulk(125.43 ppm) or nano-emulsion (85.43 ppm). The present results indicated that these novel systems could be used in integrated pest management program for A. ipsilon control.
International Journal of Natural Resource Ecology and Management, 2019
Nanotechnology is considered to be a development way to gain efficient and potential approaches t... more Nanotechnology is considered to be a development way to gain efficient and potential approaches towards pest control. The effect of both neem and peppermint oils as bulk, nano and loaded nano-emulsions have been studied against the different biological aspects of the 2 nd larval instar of Agrotis ipsilon (Hufn.) under laboratory conditions. Their effects on enzymatic activities were investigated for the 2 nd and 4 th larval instars. The effects of adding the different formulations of either neem or peppermint oils to artificial diet of 2 nd instar larvae show significant elongation of the larval duration; percentage mortalities were increased as well as larval malformations. It was recorded that nano-formulations (emulsion or loaded) significantly decreased the pupal weight and significantly increased the pupal duration, percentage pupal mortalities and pupal malformation. Adult longevity show insignificant effect, while female fecundity and percentage egg fertility show significant decrease in comparison with the control. Results of enzymatic activities show marked effects of the three formulations of either neem or peppermint oil. Significant inhibitions were recorded for amylase, invertase, trehalase, protease and alkaline phosphatase. However, significant increase in the activities of cuticle phenoloxidase and chitinase were recorded. Marked variations were recorded between nano-formulations and the bulk form.
International Journal of Natural Resource Ecology and Management, 2019
Abstract: Nanotechnology is considered to be a development way to gain efficient and potential ap... more Abstract: Nanotechnology is considered to be a development way to gain efficient and potential approaches towards pest control. The effect of both neem and peppermint oils as bulk, nano and loaded nano-emulsions have been studied against the different biological aspects of the 2nd larval instar of Agrotis ipsilon (Hufn.) under laboratory conditions. Their effects on enzymatic activities were investigated for the 2nd and 4th larval instars. The effects of adding the different formulations of either neem or peppermint oils to artificial diet of 2nd instar larvae show significant elongation of the larval duration; percentage mortalities were increased as well as larval malformations. It was recorded that nano-formulations (emulsion or loaded) significantly decreased the pupal weight and significantly increased the pupal duration, percentage pupal mortalities and pupal malformation. Adult longevity show insignificant effect, while female fecundity and percentage egg fertility show significant decrease in comparison with the control. Results of enzymatic activities show marked effects of the three formulations of either neem or peppermint oil. Significant inhibitions were recorded for amylase, invertase, trehalase, protease and alkaline phosphatase. However, significant increase in the activities of cuticle phenoloxidase and chitinase were recorded. Marked variations were recorded between nano-formulations and the bulk form.
Applications of nanotechnology in agriculture will result in the development of efficient and pot... more Applications of nanotechnology in agriculture will result in the development of efficient and potential approaches towards the management of insect pests. The toxicity effects of four essential oils peppermint, thyme, camphor and sage oils were tested against the fourth instar larvae of Agrotis ipsilon to select the most effective essential oil to be converted to the nano form. According to the results obtained, peppermint oil was the most toxic compound, which has been used in the present investigation compared with neem oil. The toxicity of bulk and nano-formulations of neem and pepper mint oils were tested against 2nd and 4th instar larvae of A. ipsilon under laboratory conditions of 25±2 °C& 65-70 % R.H.rel-ative humidity The results show that the LC50 value (the concentration used which kill 50% of the tested individuals)of loaded neem or pepper mint were lower (0.62 and 36.47 ppm) compared with neem or pepper mint oil nano-emulsion and bulk neem for the second larval instar. The different formulations of neem are more potent than in case of peppermint oil, as LC50 and LC90 values were significantly lower. The same trend was found concerning the 4th larval instar. Age of treated larvae had a detrimental effect on the response to the compounds tested. It was noticed that the younger larvae were much more sensitive to the prepared compounds compared to the older ones. The least LC50 value for loaded neem nano-emulsion was 6.68 ppm compared with the highest value for bulk neem oil (16.68 ppm). Also, LC90 values followed the same trend as in case ofLC50. Again, the toxicity of loaded peppermint oil had the most insecticidal activity as expressed by the lowest LC50 value (51.9 ppm) with more insecticidal effect than the bulk(125.43 ppm) or nano-emulsion (85.43 ppm). The present results indicated that these novel systems could be used in integrated pest management program for A. ipsilon control.
International Journal of Natural Resource Ecology and Management, 2019
Nanotechnology is considered to be a development way to gain efficient and potential approaches t... more Nanotechnology is considered to be a development way to gain efficient and potential approaches towards pest control. The effect of both neem and peppermint oils as bulk, nano and loaded nano-emulsions have been studied against the different biological aspects of the 2 nd larval instar of Agrotis ipsilon (Hufn.) under laboratory conditions. Their effects on enzymatic activities were investigated for the 2 nd and 4 th larval instars. The effects of adding the different formulations of either neem or peppermint oils to artificial diet of 2 nd instar larvae show significant elongation of the larval duration; percentage mortalities were increased as well as larval malformations. It was recorded that nano-formulations (emulsion or loaded) significantly decreased the pupal weight and significantly increased the pupal duration, percentage pupal mortalities and pupal malformation. Adult longevity show insignificant effect, while female fecundity and percentage egg fertility show significant decrease in comparison with the control. Results of enzymatic activities show marked effects of the three formulations of either neem or peppermint oil. Significant inhibitions were recorded for amylase, invertase, trehalase, protease and alkaline phosphatase. However, significant increase in the activities of cuticle phenoloxidase and chitinase were recorded. Marked variations were recorded between nano-formulations and the bulk form.
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Papers by Dalia Youssef
control. The effect of both neem and peppermint oils as bulk, nano and loaded nano-emulsions have been studied against the
different biological aspects of the 2nd larval instar of Agrotis ipsilon (Hufn.) under laboratory conditions. Their effects on
enzymatic activities were investigated for the 2nd and 4th larval instars. The effects of adding the different formulations of either
neem or peppermint oils to artificial diet of 2nd instar larvae show significant elongation of the larval duration; percentage
mortalities were increased as well as larval malformations. It was recorded that nano-formulations (emulsion or loaded)
significantly decreased the pupal weight and significantly increased the pupal duration, percentage pupal mortalities and pupal
malformation. Adult longevity show insignificant effect, while female fecundity and percentage egg fertility show significant
decrease in comparison with the control. Results of enzymatic activities show marked effects of the three formulations of either
neem or peppermint oil. Significant inhibitions were recorded for amylase, invertase, trehalase, protease and alkaline
phosphatase. However, significant increase in the activities of cuticle phenoloxidase and chitinase were recorded. Marked
variations were recorded between nano-formulations and the bulk form.
control. The effect of both neem and peppermint oils as bulk, nano and loaded nano-emulsions have been studied against the
different biological aspects of the 2nd larval instar of Agrotis ipsilon (Hufn.) under laboratory conditions. Their effects on
enzymatic activities were investigated for the 2nd and 4th larval instars. The effects of adding the different formulations of either
neem or peppermint oils to artificial diet of 2nd instar larvae show significant elongation of the larval duration; percentage
mortalities were increased as well as larval malformations. It was recorded that nano-formulations (emulsion or loaded)
significantly decreased the pupal weight and significantly increased the pupal duration, percentage pupal mortalities and pupal
malformation. Adult longevity show insignificant effect, while female fecundity and percentage egg fertility show significant
decrease in comparison with the control. Results of enzymatic activities show marked effects of the three formulations of either
neem or peppermint oil. Significant inhibitions were recorded for amylase, invertase, trehalase, protease and alkaline
phosphatase. However, significant increase in the activities of cuticle phenoloxidase and chitinase were recorded. Marked
variations were recorded between nano-formulations and the bulk form.