Analytical and Bioanalytical Chemistry, Feb 24, 2016
Book’s topic Nanotechnology has become one of the fastest growing branches of modern science, sig... more Book’s topic Nanotechnology has become one of the fastest growing branches of modern science, significantly impacting on the world’s economy and people’s lives. The unique physical and chemical properties of nanomaterials provide interesting new opportunities for their utilization in water quality management. At present, the main emphasis in this field is on water remediation, such as desalination, the removal of heavy metal ions and oil, and the elimination of pathogens, biotoxins, and water-soluble organic contaminants. Another direction is water monitoring with reliable, highly sensitive, low-cost, miniaturized, and robust biosensors that permit realtime, multi-analyte detection. Such biosensors make use of biofunctionalized smart nanomaterials such as carbon nanotubes, metal oxides, noble metals, and quantum dots (QDs) to maintain human health and environmental safety. The book Nanomaterials for water management: signal amplification for biosensing from nanostructures, which is the 4th volume of the Pan Stanford Series on the High-Tech of Biotechnology and is edited by IbrahimAbdulhalim and Robert S. Marks, is a reference work on this topic. It highlights a number of modern approaches aimed at signal amplification by collecting together articles written by experts on the subject.
This article reports current research efforts towards designing bespoke microscale extraction app... more This article reports current research efforts towards designing bespoke microscale extraction approaches exploiting the versatility of 3D printing for fast prototyping of novel geometries of sorptive devices. This is demonstrated via the so-called 3D printed spinning cup-based platform for immunoextraction of emerging contaminants using diclofenac as a model analyte. A new format of rotating cylindrical scaffold (containing a semispherical upper cavity) with enhanced coverage of biorecognition elements, and providing elevated enhancement factors with no need of eluate processing as compared with other microextraction stirring units is proposed. Two distinct synthetic routes capitalized upon modification of the acrylate surface of stereolithographic 3D printed parts with hexamethylenediamine or branched polyethyleneimine chemistries were assayed for covalent binding of monoclonal diclofenac antibody.Under the optimized experimental conditions, a LOD of 108 ng L−1 diclofenac, dynamic ...
Resumen de la publicación de una participación en formato <strong>µVídeo</strong> en ... more Resumen de la publicación de una participación en formato <strong>µVídeo</strong> en en el congreso Divulga NextGen (1ª edición) que se celebrará online, de manera gratuita y en las redes sociales los días 26, 27 y 28 de mayo de 2021.
The non-steroidal anti-inflammatory drug (NSAID) diclofenac (DCF) is found worldwide in the aqueo... more The non-steroidal anti-inflammatory drug (NSAID) diclofenac (DCF) is found worldwide in the aqueous environment. Therefore, it has raised increased public concern on potential long-term impact on human health and wildlife. The importance of DCF has been emphasized by the European Union recently by including this pharmaceutical in the first watch list of priority hazardous substances in order to gather Union-wide monitoring data. Rapid and cheap methods of analysis are therefore required for fresh and wastewater monitoring with high sample load. Here, for the first time, well-characterized monoclonal antibodies (mAbs) against DCF were generated and a highly sensitive ELISA developed. The best antibody (mAb 12G5) is highly affine (KD = 1.5 × 10(-10) M), stable to potential matrix interferences such as pH value (pH range 5.2-9.2), calcium ion concentration (up to 75 mg/L), and humic acid content (up to 20 mg/L). The limit of detection (LOD, S/N = 3) and IC50 of the ELISA calibration curve were 7.8 and 44 ng/L, respectively. The working range was defined between 11 and 180 ng/L. On average, about 10 % cross-reactivity (CR) was found for DCF metabolites 5-OH-DCF, 4&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;-OH-DCF, and DCF-acyl glucuronide, but other structurally related NSAIDs showed binding &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;1 % compared to the parent compound. While DCF concentrations at the low ppt range were measured in river and lake water, higher values of 2.9 and 2.1 μg/L were found in wastewater influents and effluents, respectively. These results could be confirmed by solid phase extraction combined with LC-MS.
... We still found an excellent recovery of 101%. Conclusions As shown with the 1-NP, the sol-gel... more ... We still found an excellent recovery of 101%. Conclusions As shown with the 1-NP, the sol-gel glass possesses ideal properties for the use as support matrix in IAC. ... IRL Press, Oxford 3. Hermanson GT, Mallia AK, Smith PK (1992) Immobi-lized affinity ligand techniques. ...
Analytical and Bioanalytical Chemistry, Feb 24, 2016
Book’s topic Nanotechnology has become one of the fastest growing branches of modern science, sig... more Book’s topic Nanotechnology has become one of the fastest growing branches of modern science, significantly impacting on the world’s economy and people’s lives. The unique physical and chemical properties of nanomaterials provide interesting new opportunities for their utilization in water quality management. At present, the main emphasis in this field is on water remediation, such as desalination, the removal of heavy metal ions and oil, and the elimination of pathogens, biotoxins, and water-soluble organic contaminants. Another direction is water monitoring with reliable, highly sensitive, low-cost, miniaturized, and robust biosensors that permit realtime, multi-analyte detection. Such biosensors make use of biofunctionalized smart nanomaterials such as carbon nanotubes, metal oxides, noble metals, and quantum dots (QDs) to maintain human health and environmental safety. The book Nanomaterials for water management: signal amplification for biosensing from nanostructures, which is the 4th volume of the Pan Stanford Series on the High-Tech of Biotechnology and is edited by IbrahimAbdulhalim and Robert S. Marks, is a reference work on this topic. It highlights a number of modern approaches aimed at signal amplification by collecting together articles written by experts on the subject.
This article reports current research efforts towards designing bespoke microscale extraction app... more This article reports current research efforts towards designing bespoke microscale extraction approaches exploiting the versatility of 3D printing for fast prototyping of novel geometries of sorptive devices. This is demonstrated via the so-called 3D printed spinning cup-based platform for immunoextraction of emerging contaminants using diclofenac as a model analyte. A new format of rotating cylindrical scaffold (containing a semispherical upper cavity) with enhanced coverage of biorecognition elements, and providing elevated enhancement factors with no need of eluate processing as compared with other microextraction stirring units is proposed. Two distinct synthetic routes capitalized upon modification of the acrylate surface of stereolithographic 3D printed parts with hexamethylenediamine or branched polyethyleneimine chemistries were assayed for covalent binding of monoclonal diclofenac antibody.Under the optimized experimental conditions, a LOD of 108 ng L−1 diclofenac, dynamic ...
Resumen de la publicación de una participación en formato <strong>µVídeo</strong> en ... more Resumen de la publicación de una participación en formato <strong>µVídeo</strong> en en el congreso Divulga NextGen (1ª edición) que se celebrará online, de manera gratuita y en las redes sociales los días 26, 27 y 28 de mayo de 2021.
The non-steroidal anti-inflammatory drug (NSAID) diclofenac (DCF) is found worldwide in the aqueo... more The non-steroidal anti-inflammatory drug (NSAID) diclofenac (DCF) is found worldwide in the aqueous environment. Therefore, it has raised increased public concern on potential long-term impact on human health and wildlife. The importance of DCF has been emphasized by the European Union recently by including this pharmaceutical in the first watch list of priority hazardous substances in order to gather Union-wide monitoring data. Rapid and cheap methods of analysis are therefore required for fresh and wastewater monitoring with high sample load. Here, for the first time, well-characterized monoclonal antibodies (mAbs) against DCF were generated and a highly sensitive ELISA developed. The best antibody (mAb 12G5) is highly affine (KD = 1.5 × 10(-10) M), stable to potential matrix interferences such as pH value (pH range 5.2-9.2), calcium ion concentration (up to 75 mg/L), and humic acid content (up to 20 mg/L). The limit of detection (LOD, S/N = 3) and IC50 of the ELISA calibration curve were 7.8 and 44 ng/L, respectively. The working range was defined between 11 and 180 ng/L. On average, about 10 % cross-reactivity (CR) was found for DCF metabolites 5-OH-DCF, 4&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;-OH-DCF, and DCF-acyl glucuronide, but other structurally related NSAIDs showed binding &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;1 % compared to the parent compound. While DCF concentrations at the low ppt range were measured in river and lake water, higher values of 2.9 and 2.1 μg/L were found in wastewater influents and effluents, respectively. These results could be confirmed by solid phase extraction combined with LC-MS.
... We still found an excellent recovery of 101%. Conclusions As shown with the 1-NP, the sol-gel... more ... We still found an excellent recovery of 101%. Conclusions As shown with the 1-NP, the sol-gel glass possesses ideal properties for the use as support matrix in IAC. ... IRL Press, Oxford 3. Hermanson GT, Mallia AK, Smith PK (1992) Immobi-lized affinity ligand techniques. ...
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