International Journal of Life Science and Pharma Research
Drug-resistant tuberculosis (DR-TB) strains are a significant threat to global TB control. Due to... more Drug-resistant tuberculosis (DR-TB) strains are a significant threat to global TB control. Due to a lack of resources and inadequate healthcare infrastructure, it is difficult to identify and monitor drug resistance situations in low and middle-income nations. The study aimed to evaluate the anti-tuberculosis therapy regimen in drug-resistant tuberculosis patients of the mountainous valley of Kashmir. The current study was carried out at Government Medical College, Department of Pulmonary Medicine, Chest Diseases Hospital (CDH), Srinagar, Kashmir. A total of 1100 suspected tuberculosis subjects were included in the study, and out of 1100 TB subjects, 195 were documented medication resistance cases. Of the total 1100 Line Probe Assay (LPA) tests done, 69 (6.2%) such samples in the Tuberculosis band were absent. Of the real samples tested by LPA, Tuberculosis band (TUB) was present in 1031 samples (93.7%), of which 24 (2.3%) were multidrugresistant (MDR), 121 (11.7%) were rifampicin m...
Sericulture is an art as well as science of rearing of silkworms to produce fibre that has fascin... more Sericulture is an art as well as science of rearing of silkworms to produce fibre that has fascinated man over millennia. Although there are various species of silkworm that have been commercialized, Bombyx mori L. is the most commonly used and intensively studied. Sericulture in Kashmir has a glorious past, which is evident from the contexts of various religious and historical manuscripts like Rajatarangini of Kalhana, Ramayana and Mahabharata . Sericulture in Kashmir has played a pivotal role in the economy as it provides a source of livelihood to a sizeable section of the society. Owing to the highly conducive climatic conditions, Jammu and Kashmir is famous for the production of bivoltine silk of optimum quality.
Actinomycetes are filamentous bacteria belonging to phyla actinobacteria and the order actinomyce... more Actinomycetes are filamentous bacteria belonging to phyla actinobacteria and the order actinomycetale being aerobic, gram-positive bacteria with high GC content in DNA and show wide distribution in nature. The actinomycetes produce an enormous variety of bioactive molecules of commercial importance. One of the first antimicrobial used was Streptomycin, produced by Streptomyces griseus. For decades, microbial natural products have been one of the major sources of novel drugs for pharmaceutical companies and today all evidence suggests that novel molecules with potential therapeutic applications are still waiting to be discovered from these natural sources, especially from actinomycetes. Any appropriate exploitation of the chemical diversity of these microbial sources relies on proper understanding of their biological diversity and other related key factors that maximize the possibility of successful identification of novel molecules. Actinomycetes have provided useful secondary metab...
Journal of Bioremediation & Biodegradation, 2016
Bioremediation means using biological agents to clean environment. Increase in the pollution has ... more Bioremediation means using biological agents to clean environment. Increase in the pollution has lead to increase in toxic substances in the environment and being referred to as most effective management tool bioremediation has tremendous future to be called as “Eco bio technology”. Hence we can infer that bioremediation is a attractive tool used at number of sites which were degraded and attained their original position with onset of this technology. Bioremediation technology uses the microbes to remediate contaminated environment and brings back it to original position. Bioremediation has also been a solution for various emerging problems.
A murine model of influenza A virus exacerbation of allergen induced airway inflammation, pulmona... more A murine model of influenza A virus exacerbation of allergen induced airway inflammation, pulmonary histopathological changes, bronchoalveolar lavage fluid (BALF) analysis, cytokine influx and the time course of these events have been studied. The present study was undertaken to determine the relative contributions of Thl/Th2 cytokines to the histopathological changes in the lungs observed at 9, 12, 24 and 48 hr following antigen challenge in mice previously immunized with influenza A virus. BALF analysis of acute phase group revealed statistically significant increase in neutrophils at 9 hr, macrophages at 12 hr, lymphocytes and eosinophils at 24 hr, as compared to OVA-sensitized control mice. These changes were associated with an alteration in the levels of IL-4, IL-5 and IFN-gamma. A peak of IL-4 at 24 hr significantly enhanced bronchiolar and perivascular histopathology, whereas increased IL-5 level peaking at 24 hr was correlated with the enhanced infiltration of eosinophils in...
Proteins in the plant apoplast are essential for many physiological processes. We have analysed a... more Proteins in the plant apoplast are essential for many physiological processes. We have analysed and compared six different infiltration solutions for proteins contained in the apoplast to recognize the most suitable method for leaves and to establish proteome maps for each extraction. The efficiency of protocols was evaluated by comparing the protein patterns resolved by 1-DE and 2-DE, and revealed distinct characteristics for each infiltration solution. Nano-LC-ESI-Q-TOF MS analysis of all fractions was applied to cover all proteins differentially extracted by infiltration solutions and led to the identification of 328 proteins in total in apoplast preparations. The predicted subcellular protein localisation distinguished the examined infiltration solutions in those with high or low amounts of intracellular protein contaminations, and with high or low quantities of secreted proteins. All tested infiltration solution extracted different subsets of proteins, and those implications on...
Acanthamoebae produce a painful, sight-threatening corneal infection. The adhesion of parasites t... more Acanthamoebae produce a painful, sight-threatening corneal infection. The adhesion of parasites to the host cells is a critical first step in the pathogenesis of infection. Subsequent to adhesion, the parasites produce a potent cytopathic effect (CPE) leading to target cell death. Recent studies showing that acanthamoebae express a mannose-binding protein (MBP) and that free α-mannose (α-Man) specifically inhibits the adhesion of parasites to host cells suggest that the MBP plays a key role in the pathogenesis of Acanthamoeba infection by mediating host-parasite interactions. However, direct evidence showing that Acanthamoeba MBP is a virulence protein has been lacking. In this study, we demonstrate that the polyclonal immunoglobulin Y (IgY) antibodies prepared against affinity-purified Acanthamoeba MBP markedly inhibit the adhesion of parasites to host cells. The antibody also inhibited the Acanthamoeba -induced CPE on host cells. In contrast, preimmune IgY did not influence either...
... ACKNOWLEDGEMENTS We thank Dr Sonal Sharma, Reader, Department of Pathology, UCMS and GTB Hosp... more ... ACKNOWLEDGEMENTS We thank Dr Sonal Sharma, Reader, Department of Pathology, UCMS and GTB Hospital, Delhi for providing help in ... For further information and details, please contact: Chairman Organizing Secretary Dr VK Vijayan Dr Ritu Kulshrestha Director, VPCI ...
Due to industrialization, the contamination of toxic metals in soils is currently one of the majo... more Due to industrialization, the contamination of toxic metals in soils is currently one of the major concerns to scientists worldwide. The presence of high concentrations of heavy metals including cadmium in the environment is mainly attributed to human activities. Being a highly toxic metal, cadmium can enter plant cell transporters usually used for the uptake of essential cations, such as iron, calcium, and zinc. This study deals with the appraisement of response and tolerance shown by various bacteria in varied cadmium concentrations (100–1,000 ppm). The optical density (OD) of the isolates was measured to determine the minimum inhibitory concentration (MIC) of cadmium. Isolated bacteria have been identified using 16S rRNA gene sequence and Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Among the 72 isolates, 07 (Bacillus pumilus, Enterobacter kobei, Klebsiella pneumonia, Pseudomonas mandelii, Pseudomonas putida, Pseudomonas avellanae, ...
International Journal of Life Science and Pharma Research
Drug-resistant tuberculosis (DR-TB) strains are a significant threat to global TB control. Due to... more Drug-resistant tuberculosis (DR-TB) strains are a significant threat to global TB control. Due to a lack of resources and inadequate healthcare infrastructure, it is difficult to identify and monitor drug resistance situations in low and middle-income nations. The study aimed to evaluate the anti-tuberculosis therapy regimen in drug-resistant tuberculosis patients of the mountainous valley of Kashmir. The current study was carried out at Government Medical College, Department of Pulmonary Medicine, Chest Diseases Hospital (CDH), Srinagar, Kashmir. A total of 1100 suspected tuberculosis subjects were included in the study, and out of 1100 TB subjects, 195 were documented medication resistance cases. Of the total 1100 Line Probe Assay (LPA) tests done, 69 (6.2%) such samples in the Tuberculosis band were absent. Of the real samples tested by LPA, Tuberculosis band (TUB) was present in 1031 samples (93.7%), of which 24 (2.3%) were multidrugresistant (MDR), 121 (11.7%) were rifampicin m...
Sericulture is an art as well as science of rearing of silkworms to produce fibre that has fascin... more Sericulture is an art as well as science of rearing of silkworms to produce fibre that has fascinated man over millennia. Although there are various species of silkworm that have been commercialized, Bombyx mori L. is the most commonly used and intensively studied. Sericulture in Kashmir has a glorious past, which is evident from the contexts of various religious and historical manuscripts like Rajatarangini of Kalhana, Ramayana and Mahabharata . Sericulture in Kashmir has played a pivotal role in the economy as it provides a source of livelihood to a sizeable section of the society. Owing to the highly conducive climatic conditions, Jammu and Kashmir is famous for the production of bivoltine silk of optimum quality.
Actinomycetes are filamentous bacteria belonging to phyla actinobacteria and the order actinomyce... more Actinomycetes are filamentous bacteria belonging to phyla actinobacteria and the order actinomycetale being aerobic, gram-positive bacteria with high GC content in DNA and show wide distribution in nature. The actinomycetes produce an enormous variety of bioactive molecules of commercial importance. One of the first antimicrobial used was Streptomycin, produced by Streptomyces griseus. For decades, microbial natural products have been one of the major sources of novel drugs for pharmaceutical companies and today all evidence suggests that novel molecules with potential therapeutic applications are still waiting to be discovered from these natural sources, especially from actinomycetes. Any appropriate exploitation of the chemical diversity of these microbial sources relies on proper understanding of their biological diversity and other related key factors that maximize the possibility of successful identification of novel molecules. Actinomycetes have provided useful secondary metab...
Journal of Bioremediation & Biodegradation, 2016
Bioremediation means using biological agents to clean environment. Increase in the pollution has ... more Bioremediation means using biological agents to clean environment. Increase in the pollution has lead to increase in toxic substances in the environment and being referred to as most effective management tool bioremediation has tremendous future to be called as “Eco bio technology”. Hence we can infer that bioremediation is a attractive tool used at number of sites which were degraded and attained their original position with onset of this technology. Bioremediation technology uses the microbes to remediate contaminated environment and brings back it to original position. Bioremediation has also been a solution for various emerging problems.
A murine model of influenza A virus exacerbation of allergen induced airway inflammation, pulmona... more A murine model of influenza A virus exacerbation of allergen induced airway inflammation, pulmonary histopathological changes, bronchoalveolar lavage fluid (BALF) analysis, cytokine influx and the time course of these events have been studied. The present study was undertaken to determine the relative contributions of Thl/Th2 cytokines to the histopathological changes in the lungs observed at 9, 12, 24 and 48 hr following antigen challenge in mice previously immunized with influenza A virus. BALF analysis of acute phase group revealed statistically significant increase in neutrophils at 9 hr, macrophages at 12 hr, lymphocytes and eosinophils at 24 hr, as compared to OVA-sensitized control mice. These changes were associated with an alteration in the levels of IL-4, IL-5 and IFN-gamma. A peak of IL-4 at 24 hr significantly enhanced bronchiolar and perivascular histopathology, whereas increased IL-5 level peaking at 24 hr was correlated with the enhanced infiltration of eosinophils in...
Proteins in the plant apoplast are essential for many physiological processes. We have analysed a... more Proteins in the plant apoplast are essential for many physiological processes. We have analysed and compared six different infiltration solutions for proteins contained in the apoplast to recognize the most suitable method for leaves and to establish proteome maps for each extraction. The efficiency of protocols was evaluated by comparing the protein patterns resolved by 1-DE and 2-DE, and revealed distinct characteristics for each infiltration solution. Nano-LC-ESI-Q-TOF MS analysis of all fractions was applied to cover all proteins differentially extracted by infiltration solutions and led to the identification of 328 proteins in total in apoplast preparations. The predicted subcellular protein localisation distinguished the examined infiltration solutions in those with high or low amounts of intracellular protein contaminations, and with high or low quantities of secreted proteins. All tested infiltration solution extracted different subsets of proteins, and those implications on...
Acanthamoebae produce a painful, sight-threatening corneal infection. The adhesion of parasites t... more Acanthamoebae produce a painful, sight-threatening corneal infection. The adhesion of parasites to the host cells is a critical first step in the pathogenesis of infection. Subsequent to adhesion, the parasites produce a potent cytopathic effect (CPE) leading to target cell death. Recent studies showing that acanthamoebae express a mannose-binding protein (MBP) and that free α-mannose (α-Man) specifically inhibits the adhesion of parasites to host cells suggest that the MBP plays a key role in the pathogenesis of Acanthamoeba infection by mediating host-parasite interactions. However, direct evidence showing that Acanthamoeba MBP is a virulence protein has been lacking. In this study, we demonstrate that the polyclonal immunoglobulin Y (IgY) antibodies prepared against affinity-purified Acanthamoeba MBP markedly inhibit the adhesion of parasites to host cells. The antibody also inhibited the Acanthamoeba -induced CPE on host cells. In contrast, preimmune IgY did not influence either...
... ACKNOWLEDGEMENTS We thank Dr Sonal Sharma, Reader, Department of Pathology, UCMS and GTB Hosp... more ... ACKNOWLEDGEMENTS We thank Dr Sonal Sharma, Reader, Department of Pathology, UCMS and GTB Hospital, Delhi for providing help in ... For further information and details, please contact: Chairman Organizing Secretary Dr VK Vijayan Dr Ritu Kulshrestha Director, VPCI ...
Due to industrialization, the contamination of toxic metals in soils is currently one of the majo... more Due to industrialization, the contamination of toxic metals in soils is currently one of the major concerns to scientists worldwide. The presence of high concentrations of heavy metals including cadmium in the environment is mainly attributed to human activities. Being a highly toxic metal, cadmium can enter plant cell transporters usually used for the uptake of essential cations, such as iron, calcium, and zinc. This study deals with the appraisement of response and tolerance shown by various bacteria in varied cadmium concentrations (100–1,000 ppm). The optical density (OD) of the isolates was measured to determine the minimum inhibitory concentration (MIC) of cadmium. Isolated bacteria have been identified using 16S rRNA gene sequence and Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Among the 72 isolates, 07 (Bacillus pumilus, Enterobacter kobei, Klebsiella pneumonia, Pseudomonas mandelii, Pseudomonas putida, Pseudomonas avellanae, ...
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Papers by Dr.Ruqeya Nazir