Abstract: Cheese analogue (Ch A) is processed cheese-like product, a nutritious food, can be heal... more Abstract: Cheese analogue (Ch A) is processed cheese-like product, a nutritious food, can be healthy and attractive when redesigned to be prepared with the addition of a natural ingredient, being Chlorella vulgaris. This microalga is recognized as a rich source of protein, fatty acids, fiber and ash. C. vulgaris also represents a valuable source of essential vitamins and minerals. Chlorella has health benefits, as assisting disorders such as gastric ulcers, wounds, constipation, anemia, hypertension, have immune-modulating and anticancer properties and is a promising ingredient in the food industries. Cheese analogue (Ch A) treatments were enriched with C. vulgaris (1, 2 and 3%) and evaluated for chemical, physical and sensory properties, within three months of cold storage. Chlorella biomass cheeses were richer in the protein, carbohydrates and fiber contents than control samples. On the other hand, addition of Chlorella biomass to the cheese gave products more firmness and gives a...
In development of fermented dairy products and protein hydrolysates with high inhibitory activity... more In development of fermented dairy products and protein hydrolysates with high inhibitory activity towards angiotensin-converting enzyme (ACE), it is crucial to have a reliable assay for measuring the ACE activity. In the present study, the performance of two commonly used assays based on synthetic N-derivates of tripeptides as substrates were tested with respect to reliability in determination of ACE activity per se and to the inhibitory effect of a tryptic whey protein digest and captopril. In one test, the ACE activity was calculated from the amount of hippuric acid liberated from hippuryl-His-Leu (HHL) during 30 min of incubation with ACE, as quantified after HPLC separation of reaction products. In the other assay, the ACE activity was measured directly as the rate of decrease in the absorbance at 340 nm during the first 30 min of ACE catalysed hydrolysis of furanacroloyl-Phe-Glu-Glu (FA-PGG). Both assays, in the absence of inhibitor, showed a good performance with relative stan...
Abstract: Cheese analogue (Ch A) is processed cheese-like product, a nutritious food, can be heal... more Abstract: Cheese analogue (Ch A) is processed cheese-like product, a nutritious food, can be healthy and attractive when redesigned to be prepared with the addition of a natural ingredient, being Chlorella vulgaris. This microalga is recognized as a rich source of protein, fatty acids, fiber and ash. C. vulgaris also represents a valuable source of essential vitamins and minerals. Chlorella has health benefits, as assisting disorders such as gastric ulcers, wounds, constipation, anemia, hypertension, have immune-modulating and anticancer properties and is a promising ingredient in the food industries. Cheese analogue (Ch A) treatments were enriched with C. vulgaris (1, 2 and 3%) and evaluated for chemical, physical and sensory properties, within three months of cold storage. Chlorella biomass cheeses were richer in the protein, carbohydrates and fiber contents than control samples. On the other hand, addition of Chlorella biomass to the cheese gave products more firmness and gives a...
In development of fermented dairy products and protein hydrolysates with high inhibitory activity... more In development of fermented dairy products and protein hydrolysates with high inhibitory activity towards angiotensin-converting enzyme (ACE), it is crucial to have a reliable assay for measuring the ACE activity. In the present study, the performance of two commonly used assays based on synthetic N-derivates of tripeptides as substrates were tested with respect to reliability in determination of ACE activity per se and to the inhibitory effect of a tryptic whey protein digest and captopril. In one test, the ACE activity was calculated from the amount of hippuric acid liberated from hippuryl-His-Leu (HHL) during 30 min of incubation with ACE, as quantified after HPLC separation of reaction products. In the other assay, the ACE activity was measured directly as the rate of decrease in the absorbance at 340 nm during the first 30 min of ACE catalysed hydrolysis of furanacroloyl-Phe-Glu-Glu (FA-PGG). Both assays, in the absence of inhibitor, showed a good performance with relative stan...
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