This study addressed the compositions in which the Heart word tugged with dragged chacters, and t... more This study addressed the compositions in which the Heart word tugged with dragged chacters, and the definition of prepositions linguistically and idiomatically, its nomenclatures, grammatically and semantically functions including rotation, expanding, inclusion and its usefulness. The study offered a model applied with the inverted word tugged with dragged character B in the Quran. The study came with certain results, which revealed the secret of Quranic systems in use of preposition, without the other, and the survival of each dragged letter on the origin of meaning, even if it was said included. Finally, with regard to the recommendations of the prepositions in the Quran, such as away from use of terms: redundant letters, moonshine, or letters droppings ;so as not said there is an excessive and launch the terms traction relevant characters or Prepositions confirmatory.
This work studied the effect of some growth regulators on date palm plantlets Barhi cv. during ac... more This work studied the effect of some growth regulators on date palm plantlets Barhi cv. during acclimatization stage to have healthy and vigorous plants. The plantlets were fertilized by nutrient solution contained 5.0 g/l complete fertilizer and different ...
This research was aimed at the enhancement of shoot regeneration via the supplementation of natur... more This research was aimed at the enhancement of shoot regeneration via the supplementation of natural compounds on Date Palm cv. Barhi. Small clusters consisting of (2-3 shoots) were used as explants and cultured on MS medium supplemented with three types of natural additives at three different concentrations: (10%, 20%, 30% for Coconut Milk and1.25g/l, 2.5g/l, 5.0g/l for Casein Hydrolysate and Yeast Extract), in addition to the control (0.05 BA mg/l, 0.1 NAA mg/l). Results showed the most efficient secondary somatic embryo formation in association with casein hydrolysate 5.00g/l and that for the growth vigor, coconut milk was the most effective component. The Yeast Extract produced the lowest readings in all assessed concentrations. Chemical analysis was also performed that tested Chlorophyll ‘A’ and ‘B’, total Carbohydrate, Protein, Amino Acid, Phenol and Indole. Coconut Milk 30% and Casein Hydrolysate 2.5g/l gave the best overall results, both in test responsiveness and regenerativ...
The present work was carried out to study the effect of ZnSO4 and CuSO4 on the in vitro multiplic... more The present work was carried out to study the effect of ZnSO4 and CuSO4 on the in vitro multiplication stage during two types of subculture intervals (4 and 6 weeks) and rooting stage of date palm cv. Yellow Maktoum. The pigment content have been estimated (chlorophyll a, b, total chlorophyll and carotenoids), protein and proline contents during multiplication stage from this study. Blending have been microelements compounds ZnSO4 or CuSO4 separately at different concentrations (0.0, 10.0, 25.0. 50.0. 75.0. 100.0 μM) to a Murashige and Skoog (MS) medium as a basal nutrient medium with recommendation of auxins and cytokinins during both multiplication and rooting stages. After three subculture from culturing on the same concentration of ZnSO4 and CuSO4 data showed that, ZnSO4 at 50 μM or CuSO4 at 25 μM induced a maximum morphogenic responses (number of shoots, number of secondary embryos, shoots length (cm) growth vigor/expaln) and physiological responses (Chl a, b, total Chl, Car co...
Hyperhydricity (or vitrification) is a fundamental physiological disorder in date palm micropropa... more Hyperhydricity (or vitrification) is a fundamental physiological disorder in date palm micropropagation. Several factors have been ascribed as being responsible for hyperhydricity, which are related to the explant, medium, culture vessel, and environment. The optimization of inorganic nutrients in the culture medium improves in vitro growth and morphogenesis, in addition to controlling hyperhydricity. This chapter describes a protocol for controlling hyperhydricity during the embryogenic callus stage by optimizing the ratio of nitrogen salts of the Murashige and Skoog (MS) nutrient culture medium. The best results of differentiation from cured hyperhydric callus are obtained using modification at a ratio of NH4+/NO3- at 10:15 (825:1425 mg/L) of the MS culture medium to remedy hyperhydric date palm callus and achieve the recovery of normal embryogenic callus and subsequent regeneration of plantlets. Based on the results of this study, nutrient medium composition has an important role in avoiding hyperhydricity problems during date palm micropropagation.
Nowadays, nanotechnology has enriched all life sciences. However, regarding plant tissue culture,... more Nowadays, nanotechnology has enriched all life sciences. However, regarding plant tissue culture, phyto nanotechnology was used to increase propagation, biomass production and plant protection. In this response, the effect of iron nanoparticles (Fe NPs) was investigated when added into the In vitro proliferation medium of date palm cv. Sewi somatic embryos with five different concentrations compared to a control. Results indicated a relatively positive impact of Fe NPs on the number of embryos and leaves when added with different doses to the culture medium. Added Fe NPs at concentrations 0.1, 1.0 and 2.0 ml/l resulted in the highest number of leaves 48.20, 52.00 and 46.33 leaf/explant, respectively with leaves’ elongation and growth improvement of shoots compared to the control. However, the highest number of somatic embryos 30.33 and 23.67 embryos/explant was recorded with using 0.5 ml/l Fe NPs in the two subcultures. Adding 1.0 ml/l Fe NPs to the culture medium resulted in maximu...
Hyperhydricity is a serious problem faced in vitro date palm propagation which directly effects o... more Hyperhydricity is a serious problem faced in vitro date palm propagation which directly effects on the commercial production. So we try to solve this problem by studying, effect of glutamine as the organic source of nitrogen and NH 4 + : NO 3-ratio as the inorganic source of nitrogen to decrease hyperhydricity phenomenon and to produce normal somatic embryos of date palm cv. Gundila. Vitrified embryonic callus were inoculated on MS basal nutrient medium modified with glutamine levels and NH 4 + : NO 3-ratio. Five concentration ratios of NH 4 : NO 3 (825:1425, 1237.5:950, 0:1900, 1650:0, 0:0 ml/l) were used with 0.1 mg/l NAA for 8 weeks throughout 2 recultures. There were gradually increasing in the percentage of vitrified embryonic callus differentiation to normal somatic embryos by increasing glutamine levels from 0.0 to 400 mg/l. Glutamine at the lowest level (50mg/l) significantly increased the number of vitrified somatic embryos. Ammonium as the sole source of N resulted in depression in somatic embryos differentiation and escalated the frequency of hyperhydricity whereas, if nitrate was used as the sole N source, somatic embryos good quality were produced and hyperhydricity was eliminated
The present work was carried out at the Central Laboratory of Date Palm Research and Development,... more The present work was carried out at the Central Laboratory of Date Palm Research and Development, Agriculture Research Center, Giza, Egypt. throughout the period from 2010 to 2013. Hyperhydricity is a serious problem during in vitro propagation of date palm which directly affects the production at the commercial level. Thus, this work aimed to reduce the hyperhydricity and improve somatic embryos of date palm cv. Gundila. throughout two experiments. The first experiment was conducted to investigate the effect of different substances of different concentrations 0.2 g/l Silver nitrate (AgNo 3), 0.05g/l Calcium nitrate (CaNo 3), 0.05g/l Calcium Chloride (CaCl 2) and 0.5 g/l Calcium Pantothenate (CaP). The second experiment was conducted in order to investigate the effect of different amino acids to improve somatic embryos. Adding 0.2g/l AgNo 3 to culture medium decreased hyperhydricity percentage to 22.22% compared with control medium (55.55%). It achieved the highest significant value of differentiation percentage (77.77%). Moreover, the superior significant results of differentiation percentage was achieved when 100mg/l Glutamine + 100mg/l Alanine was added to the regeneration medium (100%).
This study addressed the compositions in which the Heart word tugged with dragged chacters, and t... more This study addressed the compositions in which the Heart word tugged with dragged chacters, and the definition of prepositions linguistically and idiomatically, its nomenclatures, grammatically and semantically functions including rotation, expanding, inclusion and its usefulness. The study offered a model applied with the inverted word tugged with dragged character B in the Quran. The study came with certain results, which revealed the secret of Quranic systems in use of preposition, without the other, and the survival of each dragged letter on the origin of meaning, even if it was said included. Finally, with regard to the recommendations of the prepositions in the Quran, such as away from use of terms: redundant letters, moonshine, or letters droppings ;so as not said there is an excessive and launch the terms traction relevant characters or Prepositions confirmatory.
This work studied the effect of some growth regulators on date palm plantlets Barhi cv. during ac... more This work studied the effect of some growth regulators on date palm plantlets Barhi cv. during acclimatization stage to have healthy and vigorous plants. The plantlets were fertilized by nutrient solution contained 5.0 g/l complete fertilizer and different ...
This research was aimed at the enhancement of shoot regeneration via the supplementation of natur... more This research was aimed at the enhancement of shoot regeneration via the supplementation of natural compounds on Date Palm cv. Barhi. Small clusters consisting of (2-3 shoots) were used as explants and cultured on MS medium supplemented with three types of natural additives at three different concentrations: (10%, 20%, 30% for Coconut Milk and1.25g/l, 2.5g/l, 5.0g/l for Casein Hydrolysate and Yeast Extract), in addition to the control (0.05 BA mg/l, 0.1 NAA mg/l). Results showed the most efficient secondary somatic embryo formation in association with casein hydrolysate 5.00g/l and that for the growth vigor, coconut milk was the most effective component. The Yeast Extract produced the lowest readings in all assessed concentrations. Chemical analysis was also performed that tested Chlorophyll ‘A’ and ‘B’, total Carbohydrate, Protein, Amino Acid, Phenol and Indole. Coconut Milk 30% and Casein Hydrolysate 2.5g/l gave the best overall results, both in test responsiveness and regenerativ...
The present work was carried out to study the effect of ZnSO4 and CuSO4 on the in vitro multiplic... more The present work was carried out to study the effect of ZnSO4 and CuSO4 on the in vitro multiplication stage during two types of subculture intervals (4 and 6 weeks) and rooting stage of date palm cv. Yellow Maktoum. The pigment content have been estimated (chlorophyll a, b, total chlorophyll and carotenoids), protein and proline contents during multiplication stage from this study. Blending have been microelements compounds ZnSO4 or CuSO4 separately at different concentrations (0.0, 10.0, 25.0. 50.0. 75.0. 100.0 μM) to a Murashige and Skoog (MS) medium as a basal nutrient medium with recommendation of auxins and cytokinins during both multiplication and rooting stages. After three subculture from culturing on the same concentration of ZnSO4 and CuSO4 data showed that, ZnSO4 at 50 μM or CuSO4 at 25 μM induced a maximum morphogenic responses (number of shoots, number of secondary embryos, shoots length (cm) growth vigor/expaln) and physiological responses (Chl a, b, total Chl, Car co...
Hyperhydricity (or vitrification) is a fundamental physiological disorder in date palm micropropa... more Hyperhydricity (or vitrification) is a fundamental physiological disorder in date palm micropropagation. Several factors have been ascribed as being responsible for hyperhydricity, which are related to the explant, medium, culture vessel, and environment. The optimization of inorganic nutrients in the culture medium improves in vitro growth and morphogenesis, in addition to controlling hyperhydricity. This chapter describes a protocol for controlling hyperhydricity during the embryogenic callus stage by optimizing the ratio of nitrogen salts of the Murashige and Skoog (MS) nutrient culture medium. The best results of differentiation from cured hyperhydric callus are obtained using modification at a ratio of NH4+/NO3- at 10:15 (825:1425 mg/L) of the MS culture medium to remedy hyperhydric date palm callus and achieve the recovery of normal embryogenic callus and subsequent regeneration of plantlets. Based on the results of this study, nutrient medium composition has an important role in avoiding hyperhydricity problems during date palm micropropagation.
Nowadays, nanotechnology has enriched all life sciences. However, regarding plant tissue culture,... more Nowadays, nanotechnology has enriched all life sciences. However, regarding plant tissue culture, phyto nanotechnology was used to increase propagation, biomass production and plant protection. In this response, the effect of iron nanoparticles (Fe NPs) was investigated when added into the In vitro proliferation medium of date palm cv. Sewi somatic embryos with five different concentrations compared to a control. Results indicated a relatively positive impact of Fe NPs on the number of embryos and leaves when added with different doses to the culture medium. Added Fe NPs at concentrations 0.1, 1.0 and 2.0 ml/l resulted in the highest number of leaves 48.20, 52.00 and 46.33 leaf/explant, respectively with leaves’ elongation and growth improvement of shoots compared to the control. However, the highest number of somatic embryos 30.33 and 23.67 embryos/explant was recorded with using 0.5 ml/l Fe NPs in the two subcultures. Adding 1.0 ml/l Fe NPs to the culture medium resulted in maximu...
Hyperhydricity is a serious problem faced in vitro date palm propagation which directly effects o... more Hyperhydricity is a serious problem faced in vitro date palm propagation which directly effects on the commercial production. So we try to solve this problem by studying, effect of glutamine as the organic source of nitrogen and NH 4 + : NO 3-ratio as the inorganic source of nitrogen to decrease hyperhydricity phenomenon and to produce normal somatic embryos of date palm cv. Gundila. Vitrified embryonic callus were inoculated on MS basal nutrient medium modified with glutamine levels and NH 4 + : NO 3-ratio. Five concentration ratios of NH 4 : NO 3 (825:1425, 1237.5:950, 0:1900, 1650:0, 0:0 ml/l) were used with 0.1 mg/l NAA for 8 weeks throughout 2 recultures. There were gradually increasing in the percentage of vitrified embryonic callus differentiation to normal somatic embryos by increasing glutamine levels from 0.0 to 400 mg/l. Glutamine at the lowest level (50mg/l) significantly increased the number of vitrified somatic embryos. Ammonium as the sole source of N resulted in depression in somatic embryos differentiation and escalated the frequency of hyperhydricity whereas, if nitrate was used as the sole N source, somatic embryos good quality were produced and hyperhydricity was eliminated
The present work was carried out at the Central Laboratory of Date Palm Research and Development,... more The present work was carried out at the Central Laboratory of Date Palm Research and Development, Agriculture Research Center, Giza, Egypt. throughout the period from 2010 to 2013. Hyperhydricity is a serious problem during in vitro propagation of date palm which directly affects the production at the commercial level. Thus, this work aimed to reduce the hyperhydricity and improve somatic embryos of date palm cv. Gundila. throughout two experiments. The first experiment was conducted to investigate the effect of different substances of different concentrations 0.2 g/l Silver nitrate (AgNo 3), 0.05g/l Calcium nitrate (CaNo 3), 0.05g/l Calcium Chloride (CaCl 2) and 0.5 g/l Calcium Pantothenate (CaP). The second experiment was conducted in order to investigate the effect of different amino acids to improve somatic embryos. Adding 0.2g/l AgNo 3 to culture medium decreased hyperhydricity percentage to 22.22% compared with control medium (55.55%). It achieved the highest significant value of differentiation percentage (77.77%). Moreover, the superior significant results of differentiation percentage was achieved when 100mg/l Glutamine + 100mg/l Alanine was added to the regeneration medium (100%).
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Papers by Dr. Zeinab Zayed