The biological properties of an infectious bursal disease (IBD) virus isolated from bursas collec... more The biological properties of an infectious bursal disease (IBD) virus isolated from bursas collected during an outbreak in a village chicken flock in Macedonia are described. The mortality rate was 50%. Two viruses coexisted in the bursas of infected chickens (IBDVwt and IBDVtc). The virus termed IBDVtc grows on chicken embryo fibroblast (CEF) cells from the first passage. Specific pathogen free chickens inoculated with IBDVtc at passage level 4 did not develop any clinical signs of disease. Some discrete bleeding on the leg muscles was seen and the bursa of Fabricius revealed pathological lesions similar to those caused by classical strains. However, the bursa recovered quickly (bursa lesion score 2) by 14 days post infection (PI). We also found evidence of bursal repopulation by means of perinuclear antigen staining. Strong CD3 influx was evident at 4 days PI, and at 33 days PI the CD3+ cell finding was comparable to the control. The mean antibody titre was 9.2 log2at 14 days PI. ...
Background: Rabies virus can cause intensive and lethal infection of the central nervous system (... more Background: Rabies virus can cause intensive and lethal infection of the central nervous system (CNS) in animals and humans. Metabolic examinations are conducted at the cerebrospinal fluid (CSF), and it has been found that many metabolic changes occur during RABV infection. However, although it is a neurotropic virus, it can cause damage to extraneural tissues - lungs, heart, kidneys and liver. This study aimed to determine differences in metabolic, endocrinology and hematologic parameters in blood of mice after application of rabies challenge with virus standard 27 strain (CVS-27).Materials, Methods & Results: This study included 30 survived, and 30 dead mice that were part of the standard procedure of NIH (National Institute of Health) test in Pasteur Institute in Novi Sad. Tests were performed in the following order: two groups of mice were vaccinated in a 7 day period with different dilutions of standard vaccine and the examined vaccine. Seven days after the last vaccination, im...
We induced clinical form of sepsis in rats by cecal ligation and perforation (CLP) with mixed and... more We induced clinical form of sepsis in rats by cecal ligation and perforation (CLP) with mixed and pure culture of bacteria, with the aim to monitor glucose concentration in sepsis. The trial was carried out on 104 male rats, Wistar strain, body mass 190 to 240 g. The rats were divided in four groups consisting of 28 animals, and a control group of 20 animals. The period of observations were 12, 24, 72 and 120 hours after surgical intervention. In the examined sepsis model, a significant hypoglycemic activity was noticed in early sepsis (12 and 24 hours), but in the group with mixed bacteria culture only after 72 hours. Hyperglycemia was registered in late sepsis (72 hours) in the group of rats where sepsis was caused by pure bacterial culture of Escherichia coli and Staphylococcus aureus, without statistic significance compared to the findings in the control group.
On the surface and inside the body of pets there is a large number of different microorganisms. S... more On the surface and inside the body of pets there is a large number of different microorganisms. Some of them are pathogens of humans, and infect both humans and animals, while the others present only an indirect threat for humans. Skin is a large organ that plays an important role. It is in a direct contact with the environment and presents a mean of transmission for the microorganisms that may be transferred to human beings living in the cities. The objective of this paper was to carry out mycological examination of hair from dogs with clinical symptoms of skin diseases. The aim was to determine saprophytic fungi on dog skin and determine how they influence humans. In a one-year period the presence of fungi was analyzed in 72 samples from dogs with skin changes and 32 samples from dogs without lesions. The samples were inoculated on Saburo dextrose agar and incubated at 25oC for 10-21 days. The grown colonies were characterized based on their shape and color, and under a light micr...
The experiment was carried out on a mini farm of Holstein-Friesian cows with 30 animals, aged bet... more The experiment was carried out on a mini farm of Holstein-Friesian cows with 30 animals, aged between 3 and 5 years and the zinc level in serum was measured. The analyses of the blood samples indicated a variation in the concentration of zinc in serum. In 11 out of 15 tested cows (73.33%) the zinc values in serum were below the physiological values for high-milking cows. In the experimental group different thickness of the keratin layer was detected in the teat canal. The statistical analysis showed a positive correlation between the concentration of zinc in cow serum and the thickness of teat canal keratin layer. This indicates that the cows with low values of zinc have thin keratin layer of the teat canal.
In the paper are presented the results of investigating protective effect of attenuated live vacc... more In the paper are presented the results of investigating protective effect of attenuated live vaccine against classical swine fever (CSF) under experimental conditions. The vaccine contains C-strain of CSF. In the Republic of Serbia it is used for systemic immunoprophylaxis. Potency testing was performed according to the guidelines of the European Pharmacopoeia, 5th Edition, 2005 (01/2005:0065) and the guidelines published by the World Organisation for Animal Health (OIE) (Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, 5th Edition, 2008, Vol. 1, Chapter 2.8.3). The experiments were carried out on twelve weaner pigs seven weeks of age randomly assigned to two groups consisting of five, and one group consisting of two pigs. Before introducing the experimental protocol of vaccination and artificial infection, the presence of specific antibodies against CSF was controlled by ELISA test, i.e. by serum neutralisation test that proved no presence of antibodies against bovi...
Numerous studies have shown that a mare can develop a uterine infection that causes signifi cant ... more Numerous studies have shown that a mare can develop a uterine infection that causes signifi cant economic losses in horse breeding. Assessment of the reproductive system in breeding animals in our country has been rarely carried out, however this is a common or compulsory practice in countries that have developed horse breeding. Th is paper describes the importance and diff erent techniques for the use of double protected swabs in the diagnostics of bacterial infection of the mare uterus in order to improve their fertility.
Upravljanje animalnim otpadom iz stočarske proizvodnje prehrambene industrijezahteva bezbedna, ek... more Upravljanje animalnim otpadom iz stočarske proizvodnje prehrambene industrijezahteva bezbedna, ekonomski opravdana i praktična rešenja, koje su usaglašenasa propisima EU. Prema direktivi EC br. 177
The worldwide prevalence of multiple resistant Salmonella spp is described. Clonally distributed ... more The worldwide prevalence of multiple resistant Salmonella spp is described. Clonally distributed Salmonella Enteritidis PT4 and Salmonella Typhimurium DT104 are among the most pathogenic strains for humans. Recently there have been reports on the prevalence of ST ?like? monophasic 4(5),12:i strains in some countries. Vaccination strategy and antimicorbial agent therapy is also briefly discussed. Products of animal origin must be safe and without the risk of antimicrobial resistance. Subsequently, the good management practice at farm level and HACCP in feed factories are required to cope with salmonella infections. Poultry producers in developed countries have been motivated to participate in salmonella control programs, because of public awareness on safe food and risks in the food chain. Export of poultry and poultry products is more successful in the regions where Salmonella Enteritidis and Salmonella Typhimurium have been eradicated.
For over 10 years infectious laringotracheitis has occurred rarely in Vojvodina. In such situatio... more For over 10 years infectious laringotracheitis has occurred rarely in Vojvodina. In such situations morbidity was 40% and mortality was 16%. Drop in egg production was over 30% and after 4 weeks egg production returned to normal. After the disappearance of clinical symptoms and after 30 days of quarantine and disinfection of eggs with formaldehyde and 5% hydrogen peroxide there was no appearance of the disease in hatched chickens. Clinical observation and pathohistology results are valuable proof for diagnosis of ILT. Diagnosis of ILT should be confirmed by virus isolation or applying serological tests such as ELISA on sera from diseased chickens.
Aujeszky's disease (Morbus Aujeszky, pseudorabies) is an acute, contagious viral disease, of ... more Aujeszky's disease (Morbus Aujeszky, pseudorabies) is an acute, contagious viral disease, of wide range of domestic and wild-life species. Morbus Aujeszky (MA) is considered as one of the most important infectious diseases, that affect swine production and became a discriminatory factor at the international pig market. The infection in all other animal species except pigs is acute in course and inevitably fatal. The data for this research were taken from several different sources: Epizootiological Reports of Ministry of Agriculture, Forestry and Water Management, Statistical Year Book of Serbia. Also, historical, clinical and epizootiological data were obtained through questionnaire that was sent to farm owners, practicing veterinarians, veterinary inspectors and regional epizootiologists. Significant information were collected through direct epizootical surveillance on the field that encompassed anamnestic, clinical and pathological investigation as well as laboratory diagnosti...
The aim of the study was to determine whether the bacteria from the environment and from the muco... more The aim of the study was to determine whether the bacteria from the environment and from the mucous membrane of the boar prepuce have antimicrobial resistance and whether the result obtained is similar/same to the bacteria that can be found in native boar semen. The study addresses the problem of the presence of primarily resistant bacterial strains in the boar sperm, which, due to their reduced sensitivity, cannot be suppressed by antibiotics used in the semen dilution agent, as well as to emphasize the importance of microbiological monitoring of the boar mucous membranes and ambient surfaces before and during their exploitation. Such an examination could contribute to the interchangeable design of the dilution agent for the boar semen relative to the antibiotic content.Resistant strains of bacteria from prepuce swabs and swabs taken from the facility, as well as from native boar semen were isolated. The presence of these bacteria affected the quality of the semen. In conclusion, i...
The biological properties of an infectious bursal disease (IBD) virus isolated from bursas collec... more The biological properties of an infectious bursal disease (IBD) virus isolated from bursas collected during an outbreak in a village chicken flock in Macedonia are described. The mortality rate was 50%. Two viruses coexisted in the bursas of infected chickens (IBDVwt and IBDVtc). The virus termed IBDVtc grows on chicken embryo fibroblast (CEF) cells from the first passage. Specific pathogen free chickens inoculated with IBDVtc at passage level 4 did not develop any clinical signs of disease. Some discrete bleeding on the leg muscles was seen and the bursa of Fabricius revealed pathological lesions similar to those caused by classical strains. However, the bursa recovered quickly (bursa lesion score 2) by 14 days post infection (PI). We also found evidence of bursal repopulation by means of perinuclear antigen staining. Strong CD3 influx was evident at 4 days PI, and at 33 days PI the CD3+ cell finding was comparable to the control. The mean antibody titre was 9.2 log2at 14 days PI. ...
Background: Rabies virus can cause intensive and lethal infection of the central nervous system (... more Background: Rabies virus can cause intensive and lethal infection of the central nervous system (CNS) in animals and humans. Metabolic examinations are conducted at the cerebrospinal fluid (CSF), and it has been found that many metabolic changes occur during RABV infection. However, although it is a neurotropic virus, it can cause damage to extraneural tissues - lungs, heart, kidneys and liver. This study aimed to determine differences in metabolic, endocrinology and hematologic parameters in blood of mice after application of rabies challenge with virus standard 27 strain (CVS-27).Materials, Methods & Results: This study included 30 survived, and 30 dead mice that were part of the standard procedure of NIH (National Institute of Health) test in Pasteur Institute in Novi Sad. Tests were performed in the following order: two groups of mice were vaccinated in a 7 day period with different dilutions of standard vaccine and the examined vaccine. Seven days after the last vaccination, im...
We induced clinical form of sepsis in rats by cecal ligation and perforation (CLP) with mixed and... more We induced clinical form of sepsis in rats by cecal ligation and perforation (CLP) with mixed and pure culture of bacteria, with the aim to monitor glucose concentration in sepsis. The trial was carried out on 104 male rats, Wistar strain, body mass 190 to 240 g. The rats were divided in four groups consisting of 28 animals, and a control group of 20 animals. The period of observations were 12, 24, 72 and 120 hours after surgical intervention. In the examined sepsis model, a significant hypoglycemic activity was noticed in early sepsis (12 and 24 hours), but in the group with mixed bacteria culture only after 72 hours. Hyperglycemia was registered in late sepsis (72 hours) in the group of rats where sepsis was caused by pure bacterial culture of Escherichia coli and Staphylococcus aureus, without statistic significance compared to the findings in the control group.
On the surface and inside the body of pets there is a large number of different microorganisms. S... more On the surface and inside the body of pets there is a large number of different microorganisms. Some of them are pathogens of humans, and infect both humans and animals, while the others present only an indirect threat for humans. Skin is a large organ that plays an important role. It is in a direct contact with the environment and presents a mean of transmission for the microorganisms that may be transferred to human beings living in the cities. The objective of this paper was to carry out mycological examination of hair from dogs with clinical symptoms of skin diseases. The aim was to determine saprophytic fungi on dog skin and determine how they influence humans. In a one-year period the presence of fungi was analyzed in 72 samples from dogs with skin changes and 32 samples from dogs without lesions. The samples were inoculated on Saburo dextrose agar and incubated at 25oC for 10-21 days. The grown colonies were characterized based on their shape and color, and under a light micr...
The experiment was carried out on a mini farm of Holstein-Friesian cows with 30 animals, aged bet... more The experiment was carried out on a mini farm of Holstein-Friesian cows with 30 animals, aged between 3 and 5 years and the zinc level in serum was measured. The analyses of the blood samples indicated a variation in the concentration of zinc in serum. In 11 out of 15 tested cows (73.33%) the zinc values in serum were below the physiological values for high-milking cows. In the experimental group different thickness of the keratin layer was detected in the teat canal. The statistical analysis showed a positive correlation between the concentration of zinc in cow serum and the thickness of teat canal keratin layer. This indicates that the cows with low values of zinc have thin keratin layer of the teat canal.
In the paper are presented the results of investigating protective effect of attenuated live vacc... more In the paper are presented the results of investigating protective effect of attenuated live vaccine against classical swine fever (CSF) under experimental conditions. The vaccine contains C-strain of CSF. In the Republic of Serbia it is used for systemic immunoprophylaxis. Potency testing was performed according to the guidelines of the European Pharmacopoeia, 5th Edition, 2005 (01/2005:0065) and the guidelines published by the World Organisation for Animal Health (OIE) (Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, 5th Edition, 2008, Vol. 1, Chapter 2.8.3). The experiments were carried out on twelve weaner pigs seven weeks of age randomly assigned to two groups consisting of five, and one group consisting of two pigs. Before introducing the experimental protocol of vaccination and artificial infection, the presence of specific antibodies against CSF was controlled by ELISA test, i.e. by serum neutralisation test that proved no presence of antibodies against bovi...
Numerous studies have shown that a mare can develop a uterine infection that causes signifi cant ... more Numerous studies have shown that a mare can develop a uterine infection that causes signifi cant economic losses in horse breeding. Assessment of the reproductive system in breeding animals in our country has been rarely carried out, however this is a common or compulsory practice in countries that have developed horse breeding. Th is paper describes the importance and diff erent techniques for the use of double protected swabs in the diagnostics of bacterial infection of the mare uterus in order to improve their fertility.
Upravljanje animalnim otpadom iz stočarske proizvodnje prehrambene industrijezahteva bezbedna, ek... more Upravljanje animalnim otpadom iz stočarske proizvodnje prehrambene industrijezahteva bezbedna, ekonomski opravdana i praktična rešenja, koje su usaglašenasa propisima EU. Prema direktivi EC br. 177
The worldwide prevalence of multiple resistant Salmonella spp is described. Clonally distributed ... more The worldwide prevalence of multiple resistant Salmonella spp is described. Clonally distributed Salmonella Enteritidis PT4 and Salmonella Typhimurium DT104 are among the most pathogenic strains for humans. Recently there have been reports on the prevalence of ST ?like? monophasic 4(5),12:i strains in some countries. Vaccination strategy and antimicorbial agent therapy is also briefly discussed. Products of animal origin must be safe and without the risk of antimicrobial resistance. Subsequently, the good management practice at farm level and HACCP in feed factories are required to cope with salmonella infections. Poultry producers in developed countries have been motivated to participate in salmonella control programs, because of public awareness on safe food and risks in the food chain. Export of poultry and poultry products is more successful in the regions where Salmonella Enteritidis and Salmonella Typhimurium have been eradicated.
For over 10 years infectious laringotracheitis has occurred rarely in Vojvodina. In such situatio... more For over 10 years infectious laringotracheitis has occurred rarely in Vojvodina. In such situations morbidity was 40% and mortality was 16%. Drop in egg production was over 30% and after 4 weeks egg production returned to normal. After the disappearance of clinical symptoms and after 30 days of quarantine and disinfection of eggs with formaldehyde and 5% hydrogen peroxide there was no appearance of the disease in hatched chickens. Clinical observation and pathohistology results are valuable proof for diagnosis of ILT. Diagnosis of ILT should be confirmed by virus isolation or applying serological tests such as ELISA on sera from diseased chickens.
Aujeszky's disease (Morbus Aujeszky, pseudorabies) is an acute, contagious viral disease, of ... more Aujeszky's disease (Morbus Aujeszky, pseudorabies) is an acute, contagious viral disease, of wide range of domestic and wild-life species. Morbus Aujeszky (MA) is considered as one of the most important infectious diseases, that affect swine production and became a discriminatory factor at the international pig market. The infection in all other animal species except pigs is acute in course and inevitably fatal. The data for this research were taken from several different sources: Epizootiological Reports of Ministry of Agriculture, Forestry and Water Management, Statistical Year Book of Serbia. Also, historical, clinical and epizootiological data were obtained through questionnaire that was sent to farm owners, practicing veterinarians, veterinary inspectors and regional epizootiologists. Significant information were collected through direct epizootical surveillance on the field that encompassed anamnestic, clinical and pathological investigation as well as laboratory diagnosti...
The aim of the study was to determine whether the bacteria from the environment and from the muco... more The aim of the study was to determine whether the bacteria from the environment and from the mucous membrane of the boar prepuce have antimicrobial resistance and whether the result obtained is similar/same to the bacteria that can be found in native boar semen. The study addresses the problem of the presence of primarily resistant bacterial strains in the boar sperm, which, due to their reduced sensitivity, cannot be suppressed by antibiotics used in the semen dilution agent, as well as to emphasize the importance of microbiological monitoring of the boar mucous membranes and ambient surfaces before and during their exploitation. Such an examination could contribute to the interchangeable design of the dilution agent for the boar semen relative to the antibiotic content.Resistant strains of bacteria from prepuce swabs and swabs taken from the facility, as well as from native boar semen were isolated. The presence of these bacteria affected the quality of the semen. In conclusion, i...
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Papers by Dragica Stojanovic