Fed-Batch cultivation data used for model calibration, cross calibration, robustness check and va... more Fed-Batch cultivation data used for model calibration, cross calibration, robustness check and validation. (XLSX 25 kb)
Batch cultivation data used for model calibration, cross calibration, robustness check and valida... more Batch cultivation data used for model calibration, cross calibration, robustness check and validation. (XLSX 30 kb)
The infectiousness and presymptomatic transmission of SARS-CoV-2 hinder pandemic control efforts ... more The infectiousness and presymptomatic transmission of SARS-CoV-2 hinder pandemic control efforts worldwide. Therefore, the frequency of testing, accessibility, and immediate results are critical for reopening societies until an effective vaccine becomes available for a substantial proportion of the population. The loss of sense of smell is among the earliest, most discriminant, and prevalent symptoms of COVID-19, with 75-98% prevalence when clinical olfactory tests are used. Frequent screening for olfactory dysfunction could substantially reduce viral spread. However, olfactory dysfunction is generally self-reported, which is problematic as partial olfactory impairment is broadly unrecognized. To address this limitation, we developed a rapid psychophysical olfactory test (KOR) deployed on a web platform for automated reporting and traceability based on a low-cost (about USD 0.50/test), six-odor olfactory identification kit. Based on test results, we defined an anosmia score –a class...
To assess olfactory function in children and to create and validate an odor identification test t... more To assess olfactory function in children and to create and validate an odor identification test to diagnose olfactory dysfunction in children, which we called the Universal Sniff (U-Sniff) test. This is a multicenter study involving 19 countries. The U-Sniff test was developed in 3 phases including 1760 children age 5-7 years. Phase 1: identification of potentially recognizable odors; phase 2: selection of odorants for the odor identification test; and phase 3: evaluation of the test and acquisition of normative data. Test-retest reliability was evaluated in a subgroup of children (n = 27), and the test was validated using children with congenital anosmia (n = 14). Twelve odors were familiar to children and, therefore, included in the U-Sniff test. Children scored a mean ± SD of 9.88 ± 1.80 points out of 12. Normative data was obtained and reported for each country. The U-Sniff test demonstrated a high test-retest reliability (r = 0.83, P < .001) and enabled discrimination betwee...
ABSTRACT Dissolved oxygen control is critical to ensure microorganisms&#39; growth in high-ce... more ABSTRACT Dissolved oxygen control is critical to ensure microorganisms&#39; growth in high-cell-density cultures. Since biological oxygen consumption strongly depends on biomass concentration, high-cell-density cultures demand an increasing and continuous oxygen supply. This is a challenging control problem and one of the major limitations of this type of cultures. The aim of this paper is to provide a simple dissolved oxygen control strategy capable of achieving high biomass levels by manipulating three process variables: agitation, aeration and oxygenation. This strategy was developed using a split-range control scheme specially designed so that the controller action is linear with respect to the maximum transfer rate (kLa•DO*). The controller parameters were tuned through simulation and later implemented and validated in Escherichia coli fed-batch cultivations. The strategy presented here allowed reaching high biomass concentrations while maintaining oxygen concentration at an appropriate level. The implementation of this strategy in high-cell-density cultures will help improve the productivity of aerobic cultures.
In addition to gibberellic acid (GA 3 ), significant amounts of iso-GA 3 , an isomer of GA 3 , ac... more In addition to gibberellic acid (GA 3 ), significant amounts of iso-GA 3 , an isomer of GA 3 , accumulated in cultures media of the fungus Gibberella fujikuroi. It has been reported that iso-GA 3 is an intermediate in the decomposition of GA 3 to gibberellenic acid, a dicarboxylic acid lacking the ...
Journal of the Science of Food and Agriculture, 1986
Disappearance of cell wall components of untreated straw and straw treated with the ligninolytic ... more Disappearance of cell wall components of untreated straw and straw treated with the ligninolytic white‐rot fungi Phanerochaete chrysosporium, Dichomitus squalens and Cyathus stercoreus were determined during the course of rumen digestion of samples in nylon bags. The first fungus degraded hemicelluloses and cellulose non‐selectively, adversely affecting the digestion rate of crude cell walls. Dichomitus squalens and C. stercoreus preferentially degraded hemicelluloses and lignin, affording cell wall degradation rates 1.5 times higher than in native straw. Furthermore, the extent of cell wall digestion was also significantly enhanced. Both strains improved the extent of cellulose digestion, whereas the potentially degradable xylan fraction remained unchanged. Polysaccharide digestion rates were influenced in different ways depending on the strain tested: straw degraded by C. stercoreus showed an increase in cellulose digestion rate by 50%, whereas residual arabinose units were slowly...
ABSTRACTChemical composition and protein value of a tempeh‐related food using lupine as substrate... more ABSTRACTChemical composition and protein value of a tempeh‐related food using lupine as substrate was determined. Bitter lupine grits were slowly degraded as compared to sweet lupine, pointing out the inhibitory action of lupine alkaloids. Any fungal degradation of the latter could be determined. Sweet lupine tempeh was further characterized. Water‐soluble substances increased during the first 17 hr and then only slight changes occurred. The lipid fraction was preferentially metabolized by the fungus, reaching almost 50% loss after 45 hr fermentation. Linolenic and erucic acids were particularly degraded. No significant increase of the NPR values or protein digestibility of fermented lupine were observed. Preliminary results of sensory evaluation of deep‐fat fried lupine tempeh were promising.
... The concentrations of glucose, fructose, ethanol, glycerol, malic acid, citric acid and aceti... more ... The concentrations of glucose, fructose, ethanol, glycerol, malic acid, citric acid and acetic acid were measured by high-performance liquid chromatography (HPLC) using a Bio-Rad HPX-87H column, as described in Molina, Swiegers, Varela, Pretorius, and Agosin (2007). ...
Fed-Batch cultivation data used for model calibration, cross calibration, robustness check and va... more Fed-Batch cultivation data used for model calibration, cross calibration, robustness check and validation. (XLSX 25 kb)
Batch cultivation data used for model calibration, cross calibration, robustness check and valida... more Batch cultivation data used for model calibration, cross calibration, robustness check and validation. (XLSX 30 kb)
The infectiousness and presymptomatic transmission of SARS-CoV-2 hinder pandemic control efforts ... more The infectiousness and presymptomatic transmission of SARS-CoV-2 hinder pandemic control efforts worldwide. Therefore, the frequency of testing, accessibility, and immediate results are critical for reopening societies until an effective vaccine becomes available for a substantial proportion of the population. The loss of sense of smell is among the earliest, most discriminant, and prevalent symptoms of COVID-19, with 75-98% prevalence when clinical olfactory tests are used. Frequent screening for olfactory dysfunction could substantially reduce viral spread. However, olfactory dysfunction is generally self-reported, which is problematic as partial olfactory impairment is broadly unrecognized. To address this limitation, we developed a rapid psychophysical olfactory test (KOR) deployed on a web platform for automated reporting and traceability based on a low-cost (about USD 0.50/test), six-odor olfactory identification kit. Based on test results, we defined an anosmia score –a class...
To assess olfactory function in children and to create and validate an odor identification test t... more To assess olfactory function in children and to create and validate an odor identification test to diagnose olfactory dysfunction in children, which we called the Universal Sniff (U-Sniff) test. This is a multicenter study involving 19 countries. The U-Sniff test was developed in 3 phases including 1760 children age 5-7 years. Phase 1: identification of potentially recognizable odors; phase 2: selection of odorants for the odor identification test; and phase 3: evaluation of the test and acquisition of normative data. Test-retest reliability was evaluated in a subgroup of children (n = 27), and the test was validated using children with congenital anosmia (n = 14). Twelve odors were familiar to children and, therefore, included in the U-Sniff test. Children scored a mean ± SD of 9.88 ± 1.80 points out of 12. Normative data was obtained and reported for each country. The U-Sniff test demonstrated a high test-retest reliability (r = 0.83, P < .001) and enabled discrimination betwee...
ABSTRACT Dissolved oxygen control is critical to ensure microorganisms&#39; growth in high-ce... more ABSTRACT Dissolved oxygen control is critical to ensure microorganisms&#39; growth in high-cell-density cultures. Since biological oxygen consumption strongly depends on biomass concentration, high-cell-density cultures demand an increasing and continuous oxygen supply. This is a challenging control problem and one of the major limitations of this type of cultures. The aim of this paper is to provide a simple dissolved oxygen control strategy capable of achieving high biomass levels by manipulating three process variables: agitation, aeration and oxygenation. This strategy was developed using a split-range control scheme specially designed so that the controller action is linear with respect to the maximum transfer rate (kLa•DO*). The controller parameters were tuned through simulation and later implemented and validated in Escherichia coli fed-batch cultivations. The strategy presented here allowed reaching high biomass concentrations while maintaining oxygen concentration at an appropriate level. The implementation of this strategy in high-cell-density cultures will help improve the productivity of aerobic cultures.
In addition to gibberellic acid (GA 3 ), significant amounts of iso-GA 3 , an isomer of GA 3 , ac... more In addition to gibberellic acid (GA 3 ), significant amounts of iso-GA 3 , an isomer of GA 3 , accumulated in cultures media of the fungus Gibberella fujikuroi. It has been reported that iso-GA 3 is an intermediate in the decomposition of GA 3 to gibberellenic acid, a dicarboxylic acid lacking the ...
Journal of the Science of Food and Agriculture, 1986
Disappearance of cell wall components of untreated straw and straw treated with the ligninolytic ... more Disappearance of cell wall components of untreated straw and straw treated with the ligninolytic white‐rot fungi Phanerochaete chrysosporium, Dichomitus squalens and Cyathus stercoreus were determined during the course of rumen digestion of samples in nylon bags. The first fungus degraded hemicelluloses and cellulose non‐selectively, adversely affecting the digestion rate of crude cell walls. Dichomitus squalens and C. stercoreus preferentially degraded hemicelluloses and lignin, affording cell wall degradation rates 1.5 times higher than in native straw. Furthermore, the extent of cell wall digestion was also significantly enhanced. Both strains improved the extent of cellulose digestion, whereas the potentially degradable xylan fraction remained unchanged. Polysaccharide digestion rates were influenced in different ways depending on the strain tested: straw degraded by C. stercoreus showed an increase in cellulose digestion rate by 50%, whereas residual arabinose units were slowly...
ABSTRACTChemical composition and protein value of a tempeh‐related food using lupine as substrate... more ABSTRACTChemical composition and protein value of a tempeh‐related food using lupine as substrate was determined. Bitter lupine grits were slowly degraded as compared to sweet lupine, pointing out the inhibitory action of lupine alkaloids. Any fungal degradation of the latter could be determined. Sweet lupine tempeh was further characterized. Water‐soluble substances increased during the first 17 hr and then only slight changes occurred. The lipid fraction was preferentially metabolized by the fungus, reaching almost 50% loss after 45 hr fermentation. Linolenic and erucic acids were particularly degraded. No significant increase of the NPR values or protein digestibility of fermented lupine were observed. Preliminary results of sensory evaluation of deep‐fat fried lupine tempeh were promising.
... The concentrations of glucose, fructose, ethanol, glycerol, malic acid, citric acid and aceti... more ... The concentrations of glucose, fructose, ethanol, glycerol, malic acid, citric acid and acetic acid were measured by high-performance liquid chromatography (HPLC) using a Bio-Rad HPX-87H column, as described in Molina, Swiegers, Varela, Pretorius, and Agosin (2007). ...
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Papers by Eduardo Agosín