PurposeCancer incidence depends on various factors e.g., pesticide exposures which cause epigenet... more PurposeCancer incidence depends on various factors e.g., pesticide exposures which cause epigenetic alterations. The present research aimed to investigate the organochlorine pesticides (OCPs) impacts on promoter methylation of three tumor-suppressor genes and four histone modifications in thyroid nodules in 61 Papillary thyroid carcinoma (PTC) and 70 benign thyroid nodules (BTN) patients.MethodsOCPs were measured by Gas chromatography. To identify promoter methylation of TSHR, ATM, and P16 genes, the nested-methylation-specific PCR (MSP) was utilized, and histone lysine acetylation (H3K9, H4K16, and H3K18) and lysine methylation (H4K20) were detected by performing western blot analysis.ResultsFurther TSHR methylation and less P16 methylation were observed in PTC than in BTN. No substantial difference was detected for ATM methylation between PTC and BTN groups. Also, OCP dramatically increased the odds ratio of TSHR (OR=3.98, P=0.001) and P16 (OR=5.65, P<0.001) methylation while c...
Purpose: This study aimed to investigate the levels of organochlorine pesticides (OCPs) in the se... more Purpose: This study aimed to investigate the levels of organochlorine pesticides (OCPs) in the serum of Alzheimer's disease (AD) patients. Methods: 63 AD patients and 50 healthy individuals participated, and the levels of some OCPs derivatives (including; α-HCH, β-HCH, γ-HCH, 2,4-DDT, 4,4-DDT, 2,4-DDE, and 4,4-DDE), total antioxidant capacity (TAC), protein carbonyl (PC), malondialdehyde (MDA), Nitric oxide (NO) along with the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), paraoxonase 1(PON1), and acetylcholinesterase (AChE) were measured. Results: The mean OCP level of OCPs in AD patients was significantly higher than in the control group. However, the patients' mean levels of TAC, PC, MDA and activity of SOD, GPx, PON1 and AChE were significantly lower than controls. A significant positive correlation was also observed between 2,4-DDE and MDA and between γ-HCH and PC in AD patients. These findings showed that pesticide exposure is associated with an ...
Evidence-Based Complementary and Alternative Medicine
Background. Diabetes impacts various organs in the body and some reports showed that the lung is ... more Background. Diabetes impacts various organs in the body and some reports showed that the lung is also affected by diabetes, and an imbalance of inflammation and oxidative stress may participate to diabetic lung impairments. The present study is conducted to assess the impacts of Citrullus colocynthis (CC) on some aspects of these impairments. Methods. Frothy two male Wistar rats (3-4 months old and weighing 200–250 g) were used in the present research. Animals were divided into 3 groups of control, Diabetes, and Diabetes + Drug. CC was administered to diabetic rats orally. The lung tissue and BALF oxidative stress and inflammatory indices including the MDA, TAC, SOD, Gpx, TNFα, IL-6, IL-17, and IL-10 were evaluated by the ELISA method. Results. Our observations disclosed the ameliorative impacts of CC administration against oxidative stress and inflammation imbalance. Also, it was found that CC improved body weight and fasting blood sugar in rats with diabetes. Conclusion. We can co...
Additional file 2. Table S2-1. Names of the standard compounds and their retention times when inj... more Additional file 2. Table S2-1. Names of the standard compounds and their retention times when injected as separate solutions (alone) and all mixed. Figure S2-1. When the standards in the above Table were injected as a mixture, retention times changed. Fig S2-2. Chromatograms corresponding to CCAE (5 μl, green), a mixture of two ferulic acid and rutin hydrate standards (Fer A/RH, 5 μl, blue), and a combined sample of CCAE and Fer A/RH standards (50/50 v/v, 10 μl, brown). Fig S2-3. The results of spiking either the CCAE or the hydrolyzed CCAE with the standards. GA, gallic acid; RH, rutin hydrate; Fer A, ferulic acid; Cin A, Cinnamic acid; Van A, vanillic acid
Additional file 5. Figure S5. HPLC profiles of CCAE after harsh hydrolysis using 1% (A) and 5% H2... more Additional file 5. Figure S5. HPLC profiles of CCAE after harsh hydrolysis using 1% (A) and 5% H2SO4 (B)
Additional file 1. Fig. S1A. Determination of CCAE dosage. Fig. S1B. Study groups for long-term (... more Additional file 1. Fig. S1A. Determination of CCAE dosage. Fig. S1B. Study groups for long-term (28-day) experimental process. Table S1. Area under curve (AUC) for data in the above graph. Fig. S1C. The status of glucose tolerance in diabetic rats
Background and aims: Pesticides are nowadays known as one of the most important causes of human d... more Background and aims: Pesticides are nowadays known as one of the most important causes of human disorders worldwide. The aim of the present study was to investigate the role of organochlorine pesticides (OCPs) and organophosphorus pesticides (OPPs) in the development of lung cancer. Methods We determined the levels of seven derived OCP residues (α-HCH, β-HCH, γ-HCH, 2,4 DDT, 4,4 DDT, 2,4 DDE, and 4,4 DDE) and enzymatic antioxidant biomarkers including paraoxonase-1 (PON-1), erythrocyte's acetylcholinesterase (AChE), glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), and non-enzymatic antioxidant biomarkers including total antioxidant capacity (TAC), protein carbonyl (PC), malondialdehyde (MDA), and nitric oxide (NO) in the blood samples of 51 lung cancer patients and 51 healthy subjects as controls. Furthermore, the effects of OPP exposure on the development of lung cancer and oxidative stress (OS) are indirectly assessed by measuring AChE and PON-1 enzyme...
BACKGROUND Pesticides used in agriculture are some of the most common pollutants in the world. Th... more BACKGROUND Pesticides used in agriculture are some of the most common pollutants in the world. This study aimed to investigate the effects of Organophosphorus Pesticides (OPPs) and Organochlorine Pesticides (OCPs) on the families of farmworkers in the southeast of Iran. METHODS In the present case-control study, 141 family members of farmworkers (as the case group) and 59 family members of non-farmworkers (as the controls) were recruited. Serum levels of OCPs such as α-HCH, β-HCH, γ-HCH, 2,4-DDE, 4,4-DDE, 2,4-DDT, and 4,4-DDT were determined. In addition, erythrocyte acetylcholinesterase (AChE) activity, malondialdehyde (MDA), total antioxidant capacity (TAC), protein carbonyl (PC), nitric oxide (NO) serum levels, arylesterase activity of paraoxonase 1 (PON-1), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activity were determined in all participants. Furthermore, distance to farmlands, education, crops, type, and the number of consumed fruits were evaluated for each individual separately. RESULTS The erythrocyte AChE activity and serum activities of GPx, SOD, and PON-1 and TAC levels were significantly decreased, whereas the concentration of MDA, PC, NO, and seven OCPs were significantly increased in the farmworkers' families as compared to the controls. Spearman correlation and linear regression suggest that OCPs increase the oxidative stress in farmworkers' family members. Moreover, distance, education, farming precedence, products, and ventilation had significant effects on the OCP levels and increased the odds ratio of OCP levels in farmworkers' families. CONCLUSION With regards to the data obtained in this study, it was revealed that OCPs as illegal pesticides and OPPs were higher than expected in the farmworkers' family members. Furthermore, exposure to OCPs and OPPs, apart from the other effects on the body, leads to oxidative stress (OS) that may cause serious diseases in the exposed populations.
Polycystic ovarian syndrome (PCOS) is a common poignant endocrine disorder affecting women, posin... more Polycystic ovarian syndrome (PCOS) is a common poignant endocrine disorder affecting women, posing a close association with metabolic syndrome and obesity. Existing literature characterizes PCOS with deranged levels of several adipokines and myokines. CTRP15 is a paralogue of adiponectin, mainly expressed by skeletal muscles, and plays a key role in insulin, glucose, and lipid metabolism. In the current study, we aim to determine the circulating levels of CTRP15 and evaluate its association with cardiometabolic and inflammatory parameters in PCOS women. This case-control study included 120 PCOS patients (60 Recurrent pregnancy loss (RPL) and 60 infertile (inf) PCOS) and 60 healthy non-PCOS controls. Serum levels of hs-CRP were measured by commercial kits, while serum levels of adiponectin and CTRP15 were determined using the ELISA technique. Serum levels of CTRP15 were significantly elevated in PCOS-RPL and PCOS-inf subgroups when compared to controls (94.80 ± 27.08 and 87.77 ± 25.4...
Background Environmental factors namely pesticides may contribute to the development of cancer th... more Background Environmental factors namely pesticides may contribute to the development of cancer through epigenetic mechanisms. In order to evaluate the impact of organochlorine pesticides (OCPs) on promoter methylation of O6-methylguanine-DNA methyltransferase (MGMT) in thyroid cancer, this research was designed. Methods In this study, DNA methylation profile of 61 papillary thyroid carcinoma (PTC) and 70 benign thyroid nodules (BTN) subjects was carried out. In order to measure MGMT gene promoter methylation, methylation-specific PCR (MSP) was used. Results Hypermethylation of MGMT marker were found in PTC compare with BTN. Also, OCPs increased the odds ratio of methylation of MGMT (OR=3.66, P=0.001). Logistic regression analysis showed that OCPs enhance the methylation of MGMT. Conclusion The results indicated that OCPs lead to PTC development through MGMT gene promoter hypermethylation. Furthermore, MGMT promoter methylation assessment could forecast PTC incidence.
Background: The extent of metabolic disruption and the usefulness of drugs and supplements, such ... more Background: The extent of metabolic disruption and the usefulness of drugs and supplements, such as medicinal plants, in patients with diabetes may be impacted by the severity of the disease. Method: Nicotinamide + STZ together and STZ alone were used to induce early- and late-stage diabetes type 2 (ET2D and LT2D, respectively) in rats. Glucose tolerance test (GTT) was performed because the stage of disease was considered compatible with the magnitude of deviation from normal glucose tolerance test (GTT), as well as the level of FBS. Three main groups of the study were nondiabetic, early-stage diabetic, and late-stage diabetic rats. Each group was divided into two sub-groups, one of which received Citrullus colocynthis seed aqueous extract (CCAE, 90mg/kg BW) for 28 days. Weekly FBS and body weights were recorded during the study. At the end of 28 days, the serum levels of ALT, AST, ALP, TG, urea, creatinine, uric acid, cholesterol, HDL, LDL, c-peptide, and HbA1c were measured; the h...
PurposeCancer incidence depends on various factors e.g., pesticide exposures which cause epigenet... more PurposeCancer incidence depends on various factors e.g., pesticide exposures which cause epigenetic alterations. The present research aimed to investigate the organochlorine pesticides (OCPs) impacts on promoter methylation of three tumor-suppressor genes and four histone modifications in thyroid nodules in 61 Papillary thyroid carcinoma (PTC) and 70 benign thyroid nodules (BTN) patients.MethodsOCPs were measured by Gas chromatography. To identify promoter methylation of TSHR, ATM, and P16 genes, the nested-methylation-specific PCR (MSP) was utilized, and histone lysine acetylation (H3K9, H4K16, and H3K18) and lysine methylation (H4K20) were detected by performing western blot analysis.ResultsFurther TSHR methylation and less P16 methylation were observed in PTC than in BTN. No substantial difference was detected for ATM methylation between PTC and BTN groups. Also, OCP dramatically increased the odds ratio of TSHR (OR=3.98, P=0.001) and P16 (OR=5.65, P<0.001) methylation while c...
Purpose: This study aimed to investigate the levels of organochlorine pesticides (OCPs) in the se... more Purpose: This study aimed to investigate the levels of organochlorine pesticides (OCPs) in the serum of Alzheimer's disease (AD) patients. Methods: 63 AD patients and 50 healthy individuals participated, and the levels of some OCPs derivatives (including; α-HCH, β-HCH, γ-HCH, 2,4-DDT, 4,4-DDT, 2,4-DDE, and 4,4-DDE), total antioxidant capacity (TAC), protein carbonyl (PC), malondialdehyde (MDA), Nitric oxide (NO) along with the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), paraoxonase 1(PON1), and acetylcholinesterase (AChE) were measured. Results: The mean OCP level of OCPs in AD patients was significantly higher than in the control group. However, the patients' mean levels of TAC, PC, MDA and activity of SOD, GPx, PON1 and AChE were significantly lower than controls. A significant positive correlation was also observed between 2,4-DDE and MDA and between γ-HCH and PC in AD patients. These findings showed that pesticide exposure is associated with an ...
Evidence-Based Complementary and Alternative Medicine
Background. Diabetes impacts various organs in the body and some reports showed that the lung is ... more Background. Diabetes impacts various organs in the body and some reports showed that the lung is also affected by diabetes, and an imbalance of inflammation and oxidative stress may participate to diabetic lung impairments. The present study is conducted to assess the impacts of Citrullus colocynthis (CC) on some aspects of these impairments. Methods. Frothy two male Wistar rats (3-4 months old and weighing 200–250 g) were used in the present research. Animals were divided into 3 groups of control, Diabetes, and Diabetes + Drug. CC was administered to diabetic rats orally. The lung tissue and BALF oxidative stress and inflammatory indices including the MDA, TAC, SOD, Gpx, TNFα, IL-6, IL-17, and IL-10 were evaluated by the ELISA method. Results. Our observations disclosed the ameliorative impacts of CC administration against oxidative stress and inflammation imbalance. Also, it was found that CC improved body weight and fasting blood sugar in rats with diabetes. Conclusion. We can co...
Additional file 2. Table S2-1. Names of the standard compounds and their retention times when inj... more Additional file 2. Table S2-1. Names of the standard compounds and their retention times when injected as separate solutions (alone) and all mixed. Figure S2-1. When the standards in the above Table were injected as a mixture, retention times changed. Fig S2-2. Chromatograms corresponding to CCAE (5 μl, green), a mixture of two ferulic acid and rutin hydrate standards (Fer A/RH, 5 μl, blue), and a combined sample of CCAE and Fer A/RH standards (50/50 v/v, 10 μl, brown). Fig S2-3. The results of spiking either the CCAE or the hydrolyzed CCAE with the standards. GA, gallic acid; RH, rutin hydrate; Fer A, ferulic acid; Cin A, Cinnamic acid; Van A, vanillic acid
Additional file 5. Figure S5. HPLC profiles of CCAE after harsh hydrolysis using 1% (A) and 5% H2... more Additional file 5. Figure S5. HPLC profiles of CCAE after harsh hydrolysis using 1% (A) and 5% H2SO4 (B)
Additional file 1. Fig. S1A. Determination of CCAE dosage. Fig. S1B. Study groups for long-term (... more Additional file 1. Fig. S1A. Determination of CCAE dosage. Fig. S1B. Study groups for long-term (28-day) experimental process. Table S1. Area under curve (AUC) for data in the above graph. Fig. S1C. The status of glucose tolerance in diabetic rats
Background and aims: Pesticides are nowadays known as one of the most important causes of human d... more Background and aims: Pesticides are nowadays known as one of the most important causes of human disorders worldwide. The aim of the present study was to investigate the role of organochlorine pesticides (OCPs) and organophosphorus pesticides (OPPs) in the development of lung cancer. Methods We determined the levels of seven derived OCP residues (α-HCH, β-HCH, γ-HCH, 2,4 DDT, 4,4 DDT, 2,4 DDE, and 4,4 DDE) and enzymatic antioxidant biomarkers including paraoxonase-1 (PON-1), erythrocyte's acetylcholinesterase (AChE), glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), and non-enzymatic antioxidant biomarkers including total antioxidant capacity (TAC), protein carbonyl (PC), malondialdehyde (MDA), and nitric oxide (NO) in the blood samples of 51 lung cancer patients and 51 healthy subjects as controls. Furthermore, the effects of OPP exposure on the development of lung cancer and oxidative stress (OS) are indirectly assessed by measuring AChE and PON-1 enzyme...
BACKGROUND Pesticides used in agriculture are some of the most common pollutants in the world. Th... more BACKGROUND Pesticides used in agriculture are some of the most common pollutants in the world. This study aimed to investigate the effects of Organophosphorus Pesticides (OPPs) and Organochlorine Pesticides (OCPs) on the families of farmworkers in the southeast of Iran. METHODS In the present case-control study, 141 family members of farmworkers (as the case group) and 59 family members of non-farmworkers (as the controls) were recruited. Serum levels of OCPs such as α-HCH, β-HCH, γ-HCH, 2,4-DDE, 4,4-DDE, 2,4-DDT, and 4,4-DDT were determined. In addition, erythrocyte acetylcholinesterase (AChE) activity, malondialdehyde (MDA), total antioxidant capacity (TAC), protein carbonyl (PC), nitric oxide (NO) serum levels, arylesterase activity of paraoxonase 1 (PON-1), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activity were determined in all participants. Furthermore, distance to farmlands, education, crops, type, and the number of consumed fruits were evaluated for each individual separately. RESULTS The erythrocyte AChE activity and serum activities of GPx, SOD, and PON-1 and TAC levels were significantly decreased, whereas the concentration of MDA, PC, NO, and seven OCPs were significantly increased in the farmworkers' families as compared to the controls. Spearman correlation and linear regression suggest that OCPs increase the oxidative stress in farmworkers' family members. Moreover, distance, education, farming precedence, products, and ventilation had significant effects on the OCP levels and increased the odds ratio of OCP levels in farmworkers' families. CONCLUSION With regards to the data obtained in this study, it was revealed that OCPs as illegal pesticides and OPPs were higher than expected in the farmworkers' family members. Furthermore, exposure to OCPs and OPPs, apart from the other effects on the body, leads to oxidative stress (OS) that may cause serious diseases in the exposed populations.
Polycystic ovarian syndrome (PCOS) is a common poignant endocrine disorder affecting women, posin... more Polycystic ovarian syndrome (PCOS) is a common poignant endocrine disorder affecting women, posing a close association with metabolic syndrome and obesity. Existing literature characterizes PCOS with deranged levels of several adipokines and myokines. CTRP15 is a paralogue of adiponectin, mainly expressed by skeletal muscles, and plays a key role in insulin, glucose, and lipid metabolism. In the current study, we aim to determine the circulating levels of CTRP15 and evaluate its association with cardiometabolic and inflammatory parameters in PCOS women. This case-control study included 120 PCOS patients (60 Recurrent pregnancy loss (RPL) and 60 infertile (inf) PCOS) and 60 healthy non-PCOS controls. Serum levels of hs-CRP were measured by commercial kits, while serum levels of adiponectin and CTRP15 were determined using the ELISA technique. Serum levels of CTRP15 were significantly elevated in PCOS-RPL and PCOS-inf subgroups when compared to controls (94.80 ± 27.08 and 87.77 ± 25.4...
Background Environmental factors namely pesticides may contribute to the development of cancer th... more Background Environmental factors namely pesticides may contribute to the development of cancer through epigenetic mechanisms. In order to evaluate the impact of organochlorine pesticides (OCPs) on promoter methylation of O6-methylguanine-DNA methyltransferase (MGMT) in thyroid cancer, this research was designed. Methods In this study, DNA methylation profile of 61 papillary thyroid carcinoma (PTC) and 70 benign thyroid nodules (BTN) subjects was carried out. In order to measure MGMT gene promoter methylation, methylation-specific PCR (MSP) was used. Results Hypermethylation of MGMT marker were found in PTC compare with BTN. Also, OCPs increased the odds ratio of methylation of MGMT (OR=3.66, P=0.001). Logistic regression analysis showed that OCPs enhance the methylation of MGMT. Conclusion The results indicated that OCPs lead to PTC development through MGMT gene promoter hypermethylation. Furthermore, MGMT promoter methylation assessment could forecast PTC incidence.
Background: The extent of metabolic disruption and the usefulness of drugs and supplements, such ... more Background: The extent of metabolic disruption and the usefulness of drugs and supplements, such as medicinal plants, in patients with diabetes may be impacted by the severity of the disease. Method: Nicotinamide + STZ together and STZ alone were used to induce early- and late-stage diabetes type 2 (ET2D and LT2D, respectively) in rats. Glucose tolerance test (GTT) was performed because the stage of disease was considered compatible with the magnitude of deviation from normal glucose tolerance test (GTT), as well as the level of FBS. Three main groups of the study were nondiabetic, early-stage diabetic, and late-stage diabetic rats. Each group was divided into two sub-groups, one of which received Citrullus colocynthis seed aqueous extract (CCAE, 90mg/kg BW) for 28 days. Weekly FBS and body weights were recorded during the study. At the end of 28 days, the serum levels of ALT, AST, ALP, TG, urea, creatinine, uric acid, cholesterol, HDL, LDL, c-peptide, and HbA1c were measured; the h...
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Papers by Fouzieh Salimi