International Journal of Medicine and Medical Sciences, Oct 30, 2009
on Wistar rats were studied. The rats were fed with different doses of the aqueous extracts, 10, ... more on Wistar rats were studied. The rats were fed with different doses of the aqueous extracts, 10, 100, 1000, 1600, 2900 and 5000 mg/kg for 24 h and then sacrificed through cervical dislocation. Thereafter, the sera were analyzed for random blood sugar, AST, ALT, sodium, potassium, chloride, bicarbonate, urea and bilirubin, and compared with the control rats. The serum values of random blood sugar, AST, sodium, potassium, bicarbonate and urea increased progressively with increase in concentration of the extract administered to the rats. The value of chloride also increased but it was within the reference range. The bilirubin levels did not change remaining at <17 mmol/l, except in rats that died during the period of administration of the dye where the bilirubin level reached between the range of 17 - 21 mmol/l. However, serum ALT decreased with increase in concentration of the extract administered to the rats. Key words:
Background: Aqueous extract of Morinda lucida benth leaf is consumed in Southern Nigeria in the t... more Background: Aqueous extract of Morinda lucida benth leaf is consumed in Southern Nigeria in the treatment of malaria without any regard for its safety. Aim: The aim of the study was to investigate the effects of ingestion of the ethanolic leaf extract of the plant on liver and kidney functions in Wistar albino rats. Materials and Methods: Acute oral toxicity test was performed to determine the LD50; sub-chronic toxicity study was then carried out by oral administration of different doses of the extract on daily basis to different groups of rats for 42 days. The animals were subsequently sacrificed, and liver and kidney functions assessed biochemically using standard techniques. Results: The acute oral toxicity result, LD50, revealed Morinda lucida leaf extract to be non-lethal at 6400mg/kg body weight. The results obtained for liver and kidney function parameters indicated that ingestion of Morinda lucida leaf extract has no toxic effect on liver and kidney functions. Conclusion: Th...
Background: Exposure of animals to xenobiotics may or may not trigger adverse response at cellula... more Background: Exposure of animals to xenobiotics may or may not trigger adverse response at cellular levels. Aqueous extract of unripe Carica papaya is consumed by sickle cell patients as antisickling agent in Western Nigeria. Aim: This study was undertaken to investigate the effects of Carica papaya on certain organs in Wister albino rats exposed to aqueous extract of unripe Carica papaya. Materials and Methods: Different doses of aqueous extract of unripe Carica papaya were administered orally daily for 42 days to six groups of rats. At the end of exposure, the animals were sacrificed and tissue sections were prepared from livers, kidneys, hearts and small intestines using standard techniques. Results: Histopathological results showed that no pathological changes were observed in tissue sections of experimental animals when compared with tissue sections of the same organs in control animals. Conclusion: No pathological changes were elicited in the organs of rats exposed to aqueous e...
Aqueous extract of Morinda lucida benth leaf is consumed in Southern Nigeria in the treatment of ... more Aqueous extract of Morinda lucida benth leaf is consumed in Southern Nigeria in the treatment of malaria without any regard for its safety. The aim of the study was to investigate the effects of ingestion of the ethanolic leaf extract of the plant on liver and kidney functions in Wistar albino rats. Acute oral toxicity test was performed to determine the LD(50); sub-chronic toxicity study was then carried out by oral administration of different doses of the extract on daily basis to different groups of rats for 42 days. The animals were subsequently sacrificed, and liver and kidney functions assessed biochemically using standard techniques. The acute oral toxicity result, LD(50,) revealed Morinda lucida leaf extract to be non-lethal at 6400mg/kg body weight. The results obtained for liver and kidney function parameters indicated that ingestion of Morinda lucida leaf extract has no toxic effect on liver and kidney functions. The results can form the basis for clinical trials in humans.
1Department of Chemical Pathology, Obafemi Awolowo University Teaching Hospital Ile-Ife, Nigeria ... more 1Department of Chemical Pathology, Obafemi Awolowo University Teaching Hospital Ile-Ife, Nigeria 2Department of Chemical Pathology & Immunology, University College Hospital Ibadan, Nigeria 3Department of Chemical Pathology & Immunology, Olabisi Onabanjo University Ago-Iwoye, Nigeria 4Department of Family Medicine Obafemi Awolowo University Teaching Hospital Ile-Ife, Nigeria 5Department of Medical Laboratory Science, Andrews University, Michigan, MI 49104, USA
Aim: Jatropha gossypifolia belongs to the family "Euphorbiacea". The coagulant activity... more Aim: Jatropha gossypifolia belongs to the family "Euphorbiacea". The coagulant activity and the mechanism of action of Jatropha gossypifolia stem latex as a haemostatic agent were investigated. Methods: The mechanism of action was investigated using doubling dilution technique by mixing neat (undiluted) and serial dilutions of the latex with 30% bovine albumin. Results: The results of whole blood clotting time
Indian journal of clinical biochemistry : IJCB, 2014
The extract of Jatropha Gossypifolia stem was obtained by cutting the stem with a sharp knife and... more The extract of Jatropha Gossypifolia stem was obtained by cutting the stem with a sharp knife and the fluid expressed out. The suitability of the stem latex extract as a precipitant for biochemical analysis was determined. The precipitating efficacy of the extract for creatinine and protein estimation was found to be optimum at 1/4 and 1/5 dilutions respectively aqueous solution. Plasma protein was precipitated with stem extract of J. Gossypifolia at the stated dilution. The mean plasma creatinine values obtained from 0.5 % sodium tungstate as a protein precipitant were compared with the values of plasma creatinine obtained when ¼ dilution of stem extract of J. Gossypifolia was used as protein precipitant. Similarly mean cerebrospinal fluid (CSF) and urinary protein values obtained from 3 % Tricholoro-acetic acid as protein precipitant were compared with values obtained from 1/5 dilution of stem extract of J. Gossypifolia as protein precipitant. The values obtained from the stem lat...
Plasma levels of total protein, albumin and globulin were estimated in 25 HIV positive subjects a... more Plasma levels of total protein, albumin and globulin were estimated in 25 HIV positive subjects and 25 age and sex-matched controls. Test subjects were recruited from the Haematology day Clinic and Medical wards of the OAUTHC, lle-Ife. The controls were equally obtained from staff and students within the OAUTHC, lle-lfe who tested negative for H1V in the last six months. The albumin/globulin ratios were also computed for each group. This study was undertaken to determine the plasma levels of these biochemical analytes as well as their profiles in complications of Human Immunodeficiency virus /Acquired immune deficiency syndrome (HIV/AIDs) subjects. The mean plasma levels of total protein and globulin in HIV positive subjects were significantly higher than those of their respective controls (p<0.005). Mean plasma albumin in HIV positive subjects was significantly lower (p<0.005) than for control subjects. The albumin/globulin ratio in HIV/AIDS subjects was the lowest when compared with the ratio of albumin/globulin obtained from HIV-infected subjects and the controls. We concluded that HIV infection (symptomatic and asymptomatic) alters plasma levels of total proteins, albumin, and globulin and albumin/globulin ratio.
International Journal of Medicine and Medical Sciences, Oct 30, 2009
on Wistar rats were studied. The rats were fed with different doses of the aqueous extracts, 10, ... more on Wistar rats were studied. The rats were fed with different doses of the aqueous extracts, 10, 100, 1000, 1600, 2900 and 5000 mg/kg for 24 h and then sacrificed through cervical dislocation. Thereafter, the sera were analyzed for random blood sugar, AST, ALT, sodium, potassium, chloride, bicarbonate, urea and bilirubin, and compared with the control rats. The serum values of random blood sugar, AST, sodium, potassium, bicarbonate and urea increased progressively with increase in concentration of the extract administered to the rats. The value of chloride also increased but it was within the reference range. The bilirubin levels did not change remaining at <17 mmol/l, except in rats that died during the period of administration of the dye where the bilirubin level reached between the range of 17 - 21 mmol/l. However, serum ALT decreased with increase in concentration of the extract administered to the rats. Key words:
Background: Aqueous extract of Morinda lucida benth leaf is consumed in Southern Nigeria in the t... more Background: Aqueous extract of Morinda lucida benth leaf is consumed in Southern Nigeria in the treatment of malaria without any regard for its safety. Aim: The aim of the study was to investigate the effects of ingestion of the ethanolic leaf extract of the plant on liver and kidney functions in Wistar albino rats. Materials and Methods: Acute oral toxicity test was performed to determine the LD50; sub-chronic toxicity study was then carried out by oral administration of different doses of the extract on daily basis to different groups of rats for 42 days. The animals were subsequently sacrificed, and liver and kidney functions assessed biochemically using standard techniques. Results: The acute oral toxicity result, LD50, revealed Morinda lucida leaf extract to be non-lethal at 6400mg/kg body weight. The results obtained for liver and kidney function parameters indicated that ingestion of Morinda lucida leaf extract has no toxic effect on liver and kidney functions. Conclusion: Th...
Background: Exposure of animals to xenobiotics may or may not trigger adverse response at cellula... more Background: Exposure of animals to xenobiotics may or may not trigger adverse response at cellular levels. Aqueous extract of unripe Carica papaya is consumed by sickle cell patients as antisickling agent in Western Nigeria. Aim: This study was undertaken to investigate the effects of Carica papaya on certain organs in Wister albino rats exposed to aqueous extract of unripe Carica papaya. Materials and Methods: Different doses of aqueous extract of unripe Carica papaya were administered orally daily for 42 days to six groups of rats. At the end of exposure, the animals were sacrificed and tissue sections were prepared from livers, kidneys, hearts and small intestines using standard techniques. Results: Histopathological results showed that no pathological changes were observed in tissue sections of experimental animals when compared with tissue sections of the same organs in control animals. Conclusion: No pathological changes were elicited in the organs of rats exposed to aqueous e...
Aqueous extract of Morinda lucida benth leaf is consumed in Southern Nigeria in the treatment of ... more Aqueous extract of Morinda lucida benth leaf is consumed in Southern Nigeria in the treatment of malaria without any regard for its safety. The aim of the study was to investigate the effects of ingestion of the ethanolic leaf extract of the plant on liver and kidney functions in Wistar albino rats. Acute oral toxicity test was performed to determine the LD(50); sub-chronic toxicity study was then carried out by oral administration of different doses of the extract on daily basis to different groups of rats for 42 days. The animals were subsequently sacrificed, and liver and kidney functions assessed biochemically using standard techniques. The acute oral toxicity result, LD(50,) revealed Morinda lucida leaf extract to be non-lethal at 6400mg/kg body weight. The results obtained for liver and kidney function parameters indicated that ingestion of Morinda lucida leaf extract has no toxic effect on liver and kidney functions. The results can form the basis for clinical trials in humans.
1Department of Chemical Pathology, Obafemi Awolowo University Teaching Hospital Ile-Ife, Nigeria ... more 1Department of Chemical Pathology, Obafemi Awolowo University Teaching Hospital Ile-Ife, Nigeria 2Department of Chemical Pathology & Immunology, University College Hospital Ibadan, Nigeria 3Department of Chemical Pathology & Immunology, Olabisi Onabanjo University Ago-Iwoye, Nigeria 4Department of Family Medicine Obafemi Awolowo University Teaching Hospital Ile-Ife, Nigeria 5Department of Medical Laboratory Science, Andrews University, Michigan, MI 49104, USA
Aim: Jatropha gossypifolia belongs to the family "Euphorbiacea". The coagulant activity... more Aim: Jatropha gossypifolia belongs to the family "Euphorbiacea". The coagulant activity and the mechanism of action of Jatropha gossypifolia stem latex as a haemostatic agent were investigated. Methods: The mechanism of action was investigated using doubling dilution technique by mixing neat (undiluted) and serial dilutions of the latex with 30% bovine albumin. Results: The results of whole blood clotting time
Indian journal of clinical biochemistry : IJCB, 2014
The extract of Jatropha Gossypifolia stem was obtained by cutting the stem with a sharp knife and... more The extract of Jatropha Gossypifolia stem was obtained by cutting the stem with a sharp knife and the fluid expressed out. The suitability of the stem latex extract as a precipitant for biochemical analysis was determined. The precipitating efficacy of the extract for creatinine and protein estimation was found to be optimum at 1/4 and 1/5 dilutions respectively aqueous solution. Plasma protein was precipitated with stem extract of J. Gossypifolia at the stated dilution. The mean plasma creatinine values obtained from 0.5 % sodium tungstate as a protein precipitant were compared with the values of plasma creatinine obtained when ¼ dilution of stem extract of J. Gossypifolia was used as protein precipitant. Similarly mean cerebrospinal fluid (CSF) and urinary protein values obtained from 3 % Tricholoro-acetic acid as protein precipitant were compared with values obtained from 1/5 dilution of stem extract of J. Gossypifolia as protein precipitant. The values obtained from the stem lat...
Plasma levels of total protein, albumin and globulin were estimated in 25 HIV positive subjects a... more Plasma levels of total protein, albumin and globulin were estimated in 25 HIV positive subjects and 25 age and sex-matched controls. Test subjects were recruited from the Haematology day Clinic and Medical wards of the OAUTHC, lle-Ife. The controls were equally obtained from staff and students within the OAUTHC, lle-lfe who tested negative for H1V in the last six months. The albumin/globulin ratios were also computed for each group. This study was undertaken to determine the plasma levels of these biochemical analytes as well as their profiles in complications of Human Immunodeficiency virus /Acquired immune deficiency syndrome (HIV/AIDs) subjects. The mean plasma levels of total protein and globulin in HIV positive subjects were significantly higher than those of their respective controls (p<0.005). Mean plasma albumin in HIV positive subjects was significantly lower (p<0.005) than for control subjects. The albumin/globulin ratio in HIV/AIDS subjects was the lowest when compared with the ratio of albumin/globulin obtained from HIV-infected subjects and the controls. We concluded that HIV infection (symptomatic and asymptomatic) alters plasma levels of total proteins, albumin, and globulin and albumin/globulin ratio.
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